##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551704_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	863033
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.302469314614854	31.0	31.0	33.0	28.0	34.0
2	31.275961637619883	31.0	31.0	34.0	28.0	34.0
3	31.1267668791344	31.0	31.0	34.0	28.0	34.0
4	34.8993642189812	35.0	35.0	37.0	32.0	37.0
5	35.28693340810838	35.0	35.0	37.0	33.0	37.0
6	35.223310116762626	37.0	35.0	37.0	33.0	37.0
7	35.60114850764687	37.0	35.0	37.0	35.0	37.0
8	35.25627177639789	37.0	35.0	37.0	32.0	37.0
9	37.2549276794746	39.0	37.0	39.0	34.0	39.0
10	36.59134471103654	38.0	35.0	39.0	32.0	39.0
11	36.763462115585384	39.0	37.0	39.0	33.0	39.0
12	36.88454091558492	39.0	37.0	39.0	33.0	39.0
13	36.98857170003928	39.0	37.0	39.0	33.0	39.0
14	37.98646981054027	40.0	37.0	41.0	33.0	41.0
15	38.13190341504902	40.0	37.0	41.0	33.0	41.0
16	38.12819208535479	40.0	37.0	41.0	34.0	41.0
17	37.95196591555595	40.0	37.0	41.0	33.0	41.0
18	37.90149623479056	39.0	37.0	41.0	33.0	41.0
19	37.8186859598648	39.0	37.0	41.0	33.0	41.0
20	37.83411063076383	39.0	36.0	41.0	33.0	41.0
21	37.58299972306968	39.0	36.0	41.0	33.0	41.0
22	37.76733218776107	39.0	36.0	41.0	33.0	41.0
23	37.83200758256057	39.0	36.0	41.0	33.0	41.0
24	37.745545071857045	39.0	36.0	41.0	33.0	41.0
25	37.287139657463854	39.0	35.0	41.0	32.0	41.0
26	37.48379725920098	39.0	35.0	41.0	33.0	41.0
27	37.6098283611403	39.0	36.0	41.0	33.0	41.0
28	37.52013422429965	39.0	36.0	41.0	33.0	41.0
29	37.57922466464203	40.0	36.0	41.0	33.0	41.0
30	37.21635673259308	39.0	35.0	41.0	32.0	41.0
31	37.210681399204894	39.0	35.0	41.0	32.0	41.0
32	37.13335295405854	39.0	35.0	41.0	32.0	41.0
33	37.078450070854764	40.0	35.0	41.0	32.0	41.0
34	36.93831406215058	40.0	35.0	41.0	31.0	41.0
35	36.893585760915286	40.0	35.0	41.0	31.0	41.0
36	36.70597184580427	39.0	35.0	41.0	31.0	41.0
37	36.776204386159044	40.0	35.0	41.0	31.0	41.0
38	36.57782031509803	39.0	35.0	41.0	30.0	41.0
39	36.54529432825859	39.0	35.0	41.0	30.0	41.0
40	36.458936100937045	39.0	35.0	41.0	30.0	41.0
41	36.32918555837378	39.0	35.0	41.0	30.0	41.0
42	36.43860779367649	39.0	35.0	41.0	30.0	41.0
43	36.455954754916675	39.0	35.0	41.0	31.0	41.0
44	36.492246530549814	39.0	35.0	41.0	31.0	41.0
45	36.42257132693651	39.0	35.0	41.0	31.0	41.0
46	36.32998042948532	39.0	35.0	41.0	30.0	41.0
47	36.15688971337133	39.0	35.0	41.0	30.0	41.0
48	36.07513385930781	39.0	35.0	41.0	30.0	41.0
49	36.16761467985581	39.0	35.0	41.0	30.0	41.0
50	36.1114372219834	39.0	35.0	41.0	30.0	41.0
51	35.25077024864635	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	15.0
9	54.0
10	62.0
11	47.0
12	27.0
13	33.0
14	55.0
15	79.0
16	126.0
17	147.0
18	283.0
19	583.0
20	1000.0
21	1576.0
22	2278.0
23	3424.0
24	5120.0
25	7430.0
26	9541.0
27	10374.0
28	10587.0
29	11212.0
30	13020.0
31	16649.0
32	21592.0
33	30767.0
34	54534.0
35	60846.0
36	69659.0
37	109265.0
38	200576.0
39	222035.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.22480484523767	20.90221347271773	22.99390637437966	13.879075307664944
2	34.286637938526106	25.220472450068538	23.990971376529053	16.501918234876303
3	32.139790714839414	24.102438724822804	27.98525664719657	15.772513913141212
4	28.251642752942242	24.37647227858031	29.133648423640814	18.23823654483664
5	26.487979022818365	28.486164491971916	26.222288139619227	18.80356834559049
6	25.843507722184434	33.81666749707137	25.910828438773486	14.428996341970702
7	76.26220550083252	6.351553185104161	12.762432027512274	4.623809286551036
8	77.96445790601287	6.677264948153779	10.776876434620693	4.581400711212665
9	71.55890910312816	7.853581496883664	12.912947708836162	7.674561691152018
10	39.531744440826714	26.774063100715733	19.82033131989159	13.873861138565966
11	27.938213254881333	26.263306269864533	27.736482845963017	18.061997629291117
12	25.90955386410485	23.26782405771274	30.45619344799098	20.36642863019143
13	24.837636567778983	23.37025351290159	32.31185829510575	19.480251624213672
14	21.021096528174475	27.412393268855308	30.51621432784146	21.05029587512876
15	20.702568731439005	26.803957670216548	32.83084192609089	19.662631672253553
16	23.719602842533252	27.184360273593246	29.643362420672208	19.45267446320129
17	23.54023542552834	28.054315420151955	28.667849317465265	19.737599836854443
18	23.030753169345783	27.213791361396378	29.540701224634514	20.21475424462332
19	23.066325389643268	29.033072895242707	27.67483978017063	20.225761934943392
20	24.465460764536235	28.184090295504344	29.349399153914163	18.00104978604526
21	24.61910494731951	28.406445639969736	29.586354171856694	17.388095240854057
22	23.724817011632233	24.04925420001321	30.59153010371562	21.634398684638942
23	23.012677383135987	26.32935241178495	30.65699689351392	20.000973311565144
24	23.01499479162442	25.73389430068143	30.488289555555813	20.76282135213833
25	22.259519624394432	28.74443966800806	27.910056741746843	21.08598396585067
26	21.749921497787454	27.73416543747458	28.878385878639634	21.63752718609833
27	22.545835443140643	27.416332863285646	29.10711409644822	20.930717597125486
28	20.055895892741066	28.340051886776056	29.542555151425265	22.061497069057616
29	21.717941260647045	26.36712617014645	29.127391420722038	22.787541148484472
30	23.13735395981382	27.324447616719176	28.877922396941948	20.660276026525057
31	23.310580244324377	27.506943535183474	26.874059276991723	22.308416943500422
32	23.949026282888372	27.940414792945344	27.363264208900475	20.747294715265813
33	23.477781266765003	27.32410000544591	27.168138414174198	22.029980313614892
34	21.26859575473939	27.19131249905855	28.564840510154305	22.975251236047754
35	21.822572253899907	26.261104731800522	29.184979021659657	22.73134399263991
36	21.291074617077214	29.26064240880708	28.37156864221878	21.076714331896927
37	22.477819504005062	27.931956251962553	28.722656028216765	20.86756821581562
38	21.693376730669627	29.18196639062469	27.712729408956555	21.411927469749127
39	23.148941002255995	26.355886738977535	27.961850821463376	22.53332143730309
40	22.334140177722055	26.04581748322486	29.295403536133612	22.324638802919473
41	20.395280365872452	26.459474898410605	29.53004114558771	23.61520359012923
42	21.30196643697286	26.37222446882101	30.032687046729382	22.29312204747675
43	22.02013132753904	26.148594549686976	28.84559454852827	22.98567957424571
44	22.048635451946797	26.172811468391128	27.958142967881876	23.8204101117802
45	22.64200789541072	25.152688251781797	27.415058288617004	24.790245564190478
46	22.87363287382985	26.529344764336937	27.601957283209334	22.995065078623877
47	20.687273835415333	25.908395159860632	30.645989203193853	22.758341801530186
48	21.139400231509107	25.492072724913186	30.152033583883814	23.216493459693893
49	21.729760043938064	24.733584926648227	30.83694366264094	22.699711366772764
50	20.695384765124857	24.757685974927956	30.127353183481976	24.419576076465212
51	20.45437428232756	24.128046088620017	29.553678712169756	25.86390091688267
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	177.0
1	664.0
2	1151.0
3	5600.0
4	10049.0
5	6892.5
6	3736.0
7	3682.5
8	3629.0
9	3551.0
10	3473.0
11	3466.0
12	3459.0
13	3335.0
14	3211.0
15	3156.5
16	3102.0
17	2925.5
18	2749.0
19	2791.0
20	2833.0
21	3184.5
22	3536.0
23	3691.0
24	3846.0
25	4751.5
26	6886.5
27	8116.0
28	9864.0
29	11612.0
30	13648.5
31	15685.0
32	18023.5
33	20362.0
34	22496.5
35	24631.0
36	27001.5
37	29372.0
38	31972.0
39	34572.0
40	37721.5
41	40871.0
42	43364.0
43	45857.0
44	49988.5
45	54120.0
46	67769.5
47	81419.0
48	80957.0
49	80495.0
50	78303.5
51	76112.0
52	68092.5
53	60073.0
54	54593.0
55	49113.0
56	45114.5
57	41116.0
58	38278.5
59	35441.0
60	33316.5
61	31192.0
62	28091.5
63	24991.0
64	21194.0
65	17397.0
66	13645.5
67	9894.0
68	8526.5
69	7159.0
70	5905.5
71	4652.0
72	4159.5
73	3667.0
74	2816.5
75	1619.5
76	1273.0
77	956.0
78	639.0
79	500.0
80	361.0
81	254.0
82	147.0
83	108.0
84	69.0
85	50.5
86	32.0
87	21.0
88	10.0
89	7.5
90	5.0
91	4.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	863033.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.20103068764295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.24339835617258	26.13444143890531
2	8.8607066048567	6.238120102235225
3	3.215885673873658	3.3960747088193384
4	1.7659971102245553	2.4865967388521333
5	1.3367069426260638	2.352673105388273
6	1.0872670244885414	2.2963751936810035
7	0.8769042955320269	2.160755451200416
8	0.8261534358572931	2.326516195865139
9	0.7212758636820932	2.2850688430556576
>10	6.965722533189778	43.26572202739092
>50	0.06145084465452081	1.4419326261977619
>100	0.032220151204412316	1.9745299387807207
>500	0.003653831579877746	0.8519230848550219
>1k	0.0023251655508312926	2.127703043522761
>5k	3.321665072616132E-4	0.661567501250304
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5658	0.6555948613784178	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTC	4919	0.5699666177307241	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGC	3622	0.4196826772556785	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCG	3274	0.37935976955689993	No Hit
GCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	2543	0.2946584893045805	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTT	1419	0.16442013225450244	No Hit
GAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT	1229	0.1424047516143647	No Hit
CTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCT	1191	0.13800167548633716	TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	937	0.10857058768320561	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1587042442177762E-4	0.0	0.0	0.14252062203878646	0.0
2	1.1587042442177762E-4	0.0	0.0	0.688270321065359	0.0
3	1.1587042442177762E-4	0.0	0.0	0.9619562635495977	0.0
4	1.1587042442177762E-4	0.0	0.0	1.4767685592555557	0.0
5	1.1587042442177762E-4	0.0	0.0	2.9593306397322	0.0
6	1.1587042442177762E-4	0.0	0.0	3.9417959684044526	0.0
7	1.1587042442177762E-4	0.0	0.0	4.627980621830219	0.0
8	1.1587042442177762E-4	0.0	0.0	5.517633740540628	0.0
9	1.1587042442177762E-4	0.0	0.0	5.868141774416506	0.0
10	1.1587042442177762E-4	0.0	0.0	6.913872354823049	0.0
11	1.1587042442177762E-4	0.0	0.0	7.965628197299524	0.0
12	1.1587042442177762E-4	0.0	0.0	9.154111140593697	0.0
13	1.1587042442177762E-4	0.0	0.0	9.528604352324882	0.0
14	1.1587042442177762E-4	0.0	0.0	9.715039865219522	0.0
15	1.1587042442177762E-4	0.0	0.0	10.041330980391248	0.0
16	1.1587042442177762E-4	0.0	0.0	10.570858819998772	0.0
17	1.1587042442177762E-4	0.0	0.0	11.20165741055093	0.0
18	1.1587042442177762E-4	0.0	0.0	11.836743206806693	0.0
19	1.1587042442177762E-4	0.0	0.0	12.396629097612722	0.0
20	1.1587042442177762E-4	0.0	0.0	12.86265994463711	0.0
21	1.1587042442177762E-4	0.0	0.0	13.449543644333415	0.0
22	1.1587042442177762E-4	0.0	0.0	14.08080571658326	0.0
23	1.1587042442177762E-4	0.0	0.0	14.695730058989634	0.0
24	1.1587042442177762E-4	0.0	0.0	15.169755965299125	0.0
25	1.1587042442177762E-4	0.0	0.0	15.605544631549432	0.0
26	1.1587042442177762E-4	0.0	0.0	16.048401393689463	0.0
27	2.3174084884355523E-4	0.0	0.0	16.470517349858	0.0
28	2.3174084884355523E-4	0.0	0.0	16.91383759369572	0.0
29	2.3174084884355523E-4	0.0	0.0	17.380216051993376	0.0
30	2.3174084884355523E-4	0.0	0.0	17.890277660298043	0.0
31	3.4761127326533284E-4	0.0	0.0	18.37056056952631	0.0
32	3.4761127326533284E-4	0.0	0.0	18.844818216684647	0.0
33	3.4761127326533284E-4	0.0	0.0	19.310733193284612	0.0
34	3.4761127326533284E-4	0.0	0.0	19.776995781157847	0.0
35	3.4761127326533284E-4	0.0	0.0	20.248009056432373	0.0
36	3.4761127326533284E-4	0.0	0.0	20.72226670359071	0.0
37	3.4761127326533284E-4	0.0	0.0	21.214252525685577	0.0
38	3.4761127326533284E-4	0.0	0.0	21.693376730669627	0.0
39	3.4761127326533284E-4	0.0	0.0	22.21954432796892	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTAG	25	3.8899958E-5	45.0	1
GTATACG	25	3.8899958E-5	45.0	1
CGGTCTA	40	6.8121153E-9	45.0	31
GACTACG	20	7.0323097E-4	45.0	1
TCGAACG	30	2.164872E-6	44.999996	1
CGTTTTT	3710	0.0	42.99865	1
CTACGAA	70	0.0	41.785717	11
TATAGCG	60	3.6379788E-12	41.249996	1
ACACGAC	180	0.0	40.0	26
CACGACC	180	0.0	40.0	27
TGATGCG	45	1.9274012E-8	40.0	1
AACACGT	195	0.0	39.23077	41
CGAATAT	75	0.0	39.0	14
TCGCGCG	35	6.2464787E-6	38.57143	1
GCGAGAC	200	0.0	38.25	21
TCAAGCG	200	0.0	38.25	17
GCGTAAG	90	0.0	37.5	1
GCCGAAT	30	1.1396864E-4	37.499996	17
CGTTTAC	30	1.1396864E-4	37.499996	33
CGCCCCG	30	1.1396864E-4	37.499996	25
>>END_MODULE