FastQCFastQC Report
Sat 18 Jun 2016
SRR3551703_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551703_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966950
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC61810.6392264336315218No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49310.5099539790061534No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGC47150.48761569884688977No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCG42570.44025027147215473No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC31180.322457210817519No Hit
GAATGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTT16830.17405243290759606No Hit
GAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT14910.15419618387713946No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT11770.12172294327524691No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC11410.1179998965820363No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACC207.03273E-445.00000413
CGTACTC207.03273E-445.00000440
TATCGCG253.8903458E-545.032
TATAGCG302.165143E-644.9999961
CGTTTTT24650.040.8012161
ATAACGG501.0804797E-940.52
CGGTCTA1000.038.2531
GTTAGCG659.094947E-1238.076921
TATTACG301.139788E-437.4999961
TACGGCT5250.037.2857177
CGAATAT1150.037.17391214
TTACACG5400.037.08333234
ACAACGA2800.036.96428313
TCACGAC1050.036.4285725
CTATACG250.002106943936.01
CGGTTAC250.002106943936.045
CCTCGCA250.002106943936.043
CTACGAA1250.036.011
TTGCGCA250.002106943936.019
CTACCGT250.002106943936.020