##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551703_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	966950
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.329728527845287	31.0	31.0	33.0	28.0	34.0
2	31.30821655721599	31.0	31.0	34.0	28.0	34.0
3	31.16126687005533	31.0	31.0	34.0	28.0	34.0
4	34.97292310874399	35.0	35.0	37.0	32.0	37.0
5	35.314159987589846	35.0	35.0	37.0	33.0	37.0
6	35.23150524846166	37.0	35.0	37.0	33.0	37.0
7	35.60185635244842	37.0	35.0	37.0	35.0	37.0
8	35.18792388437872	37.0	35.0	37.0	32.0	37.0
9	37.11301928745023	39.0	37.0	39.0	33.0	39.0
10	36.56238998914112	38.0	35.0	39.0	32.0	39.0
11	36.78150473137184	39.0	37.0	39.0	33.0	39.0
12	36.84185945498733	39.0	35.0	39.0	33.0	39.0
13	36.883674440250275	39.0	37.0	39.0	33.0	39.0
14	37.79478876880914	40.0	37.0	41.0	33.0	41.0
15	37.960467449195924	40.0	37.0	41.0	33.0	41.0
16	37.997352500129274	40.0	37.0	41.0	33.0	41.0
17	37.86643983659962	40.0	37.0	41.0	33.0	41.0
18	37.83081958736233	39.0	37.0	41.0	33.0	41.0
19	37.775392729717154	39.0	37.0	41.0	33.0	41.0
20	37.79682300015513	39.0	36.0	41.0	33.0	41.0
21	37.54473240601892	39.0	36.0	41.0	33.0	41.0
22	37.73863178034024	39.0	36.0	41.0	33.0	41.0
23	37.81391902373442	40.0	36.0	41.0	33.0	41.0
24	37.72980815967733	40.0	36.0	41.0	33.0	41.0
25	37.27148456486892	39.0	35.0	41.0	32.0	41.0
26	37.462828481307206	39.0	35.0	41.0	33.0	41.0
27	37.5739210920937	39.0	36.0	41.0	33.0	41.0
28	37.47473602564766	39.0	35.0	41.0	33.0	41.0
29	37.53972077149801	40.0	36.0	41.0	33.0	41.0
30	37.21986969336574	39.0	35.0	41.0	32.0	41.0
31	37.25575365841046	39.0	35.0	41.0	32.0	41.0
32	37.235350328352034	39.0	35.0	41.0	32.0	41.0
33	37.257237706189564	40.0	35.0	41.0	33.0	41.0
34	37.14312942758157	40.0	35.0	41.0	32.0	41.0
35	37.09476188013858	40.0	35.0	41.0	32.0	41.0
36	36.9229205232949	39.0	35.0	41.0	31.0	41.0
37	36.96832204353896	39.0	35.0	41.0	32.0	41.0
38	36.80794973886964	39.0	35.0	41.0	31.0	41.0
39	36.77745695227261	39.0	35.0	41.0	31.0	41.0
40	36.7069424479032	39.0	35.0	41.0	31.0	41.0
41	36.49807125497699	39.0	35.0	41.0	30.0	41.0
42	36.626993122705414	39.0	35.0	41.0	31.0	41.0
43	36.637130151507314	39.0	35.0	41.0	31.0	41.0
44	36.6972470138063	39.0	35.0	41.0	31.0	41.0
45	36.647502973266455	39.0	35.0	41.0	31.0	41.0
46	36.56347174104142	39.0	35.0	41.0	31.0	41.0
47	36.40910181498526	39.0	35.0	41.0	31.0	41.0
48	36.33468638502508	39.0	35.0	41.0	31.0	41.0
49	36.422618542840894	39.0	35.0	41.0	31.0	41.0
50	36.36676043228709	39.0	35.0	41.0	31.0	41.0
51	35.50147887688092	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	31.0
9	50.0
10	72.0
11	57.0
12	64.0
13	43.0
14	52.0
15	72.0
16	112.0
17	176.0
18	354.0
19	601.0
20	1058.0
21	1675.0
22	2490.0
23	3477.0
24	4905.0
25	6543.0
26	8428.0
27	9438.0
28	10426.0
29	12143.0
30	14626.0
31	18923.0
32	25497.0
33	37868.0
34	67395.0
35	69197.0
36	78730.0
37	121609.0
38	219895.0
39	250893.0
40	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.09519623558612	20.384094317182893	21.750452453591187	12.770256993639794
2	33.611458710377995	25.5094885981695	23.456021510936452	17.423031180516055
3	32.90180464346657	25.050312839340194	25.913852836237655	16.13402968095558
4	29.329334505403587	24.49775065928952	27.1219814881845	19.050933347122395
5	27.519933812503233	28.36744402502715	24.29701639174725	19.815605770722375
6	26.741506799731113	34.78339107502973	23.92295361704328	14.552148508195872
7	76.1411655204509	6.819380526397436	12.049330368685041	4.990123584466622
8	76.50126687005533	7.752003723046694	10.454108278607995	5.2926211282899835
9	69.79275040074461	8.355757795129014	12.69838150886809	9.153110295258287
10	41.4398883085992	23.59718703138735	19.938673147525726	15.02425151248772
11	30.987848389265217	25.271834117586224	25.08495785717979	18.655359635968768
12	28.08459589430684	22.974610889911578	28.579140596721654	20.36165261905993
13	26.12182636123895	23.0175293448472	31.169760587414036	19.690883706499818
14	21.05631108123481	26.580691866177155	30.17291483530689	22.190082217281144
15	20.52991364600031	26.513780443663066	32.81121050726511	20.145095403071515
16	24.148404777909924	26.366306427426444	29.402657841667097	20.082630952996535
17	24.055535446507058	27.453746315735046	27.861626764569003	20.629091473188893
18	24.05894823930917	26.49630280779772	28.77491080200631	20.66983815088681
19	24.04571073995553	27.51610734784632	27.36129065618698	21.07689125601117
20	25.557371115362738	27.800299912094733	28.46227829774032	18.180050674802214
21	24.832411189823674	27.73690470034645	29.436165261905995	17.994518847923885
22	23.811365634210663	24.38254304772739	30.05491493872486	21.751176379337092
23	23.49190754434045	26.309323129427582	30.137028801902893	20.061740524329075
24	23.414654325456333	25.5073168209318	29.24628988055225	21.83173897305962
25	22.867986969336574	27.991519726976577	27.22033197166348	21.920161332023373
26	21.72883809917783	26.566730441077613	27.801954599513934	23.902476860230625
27	22.49878483892652	27.4753606701484	28.555147629143185	21.470706861781892
28	19.779823155282074	27.840012410155644	29.251460778737265	23.128703655825017
29	21.377010186669423	26.959822121102434	28.226485340503643	23.436682351724496
30	22.099901752934485	26.862816071151556	29.30492786597032	21.732354309943638
31	22.80479859351569	25.856249030456592	28.71865142975335	22.62030094627437
32	23.268317906820414	26.633745281555406	28.31066756295569	21.787269248668494
33	23.18909974662599	25.73545684885465	27.478980298877914	23.596463105641448
34	20.871710016029784	25.17472464967165	30.795284140855266	23.1582811934433
35	21.013392626299186	26.183256631676922	28.398779668028336	24.404571073995555
36	22.241584363203888	27.794818760018615	27.060758053673922	22.902838823103572
37	21.135736077356636	27.76100108588862	29.352500129272453	21.75076270748229
38	21.335022493407106	26.72568385128497	28.274884947515382	23.664408707792546
39	22.402709550648947	24.127410931278764	29.149180412637676	24.320699105434613
40	22.537566575314134	23.718289466880396	29.970629298309113	23.773514659496357
41	20.696313149594083	25.534412327421276	28.77884068462692	24.990433838357724
42	21.503904028129686	25.418273954185842	29.071823775789856	24.005998241894616
43	22.590619990692385	24.75453746315735	28.826723201820155	23.82811934433011
44	22.06018925487357	24.39660789079063	28.795284140855266	24.747918713480534
45	21.795025595946015	23.6141475774342	28.146336418635915	26.444490407983867
46	21.981798438388747	25.903200785976527	27.814881844976476	24.300118930658254
47	20.211696571694503	25.31516624437665	31.323232845545274	23.14990433838358
48	20.690831997517968	25.116190082217283	30.19525311546616	23.997724804798594
49	21.717462123170794	24.001861523346605	30.661874967681886	23.618801385800715
50	20.265784166709757	24.50860954547805	30.481410621024875	24.744195666787324
51	19.929158694865297	23.584880293707016	29.218470448316875	27.26749056311081
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	245.0
1	848.0
2	1451.0
3	4906.5
4	8362.0
5	5596.5
6	2831.0
7	2692.5
8	2554.0
9	2533.0
10	2512.0
11	2438.0
12	2364.0
13	2359.5
14	2355.0
15	2363.0
16	2371.0
17	2316.0
18	2261.0
19	2368.5
20	2476.0
21	2994.5
22	3513.0
23	3964.0
24	4415.0
25	5353.5
26	7686.0
27	9080.0
28	10812.5
29	12545.0
30	14950.0
31	17355.0
32	19355.5
33	21356.0
34	24358.0
35	27360.0
36	29147.5
37	30935.0
38	34261.0
39	37587.0
40	40165.0
41	42743.0
42	46460.5
43	50178.0
44	53053.5
45	55929.0
46	73367.5
47	90806.0
48	88839.5
49	86873.0
50	85962.5
51	85052.0
52	76701.0
53	68350.0
54	62515.5
55	56681.0
56	53607.5
57	50534.0
58	47707.0
59	44880.0
60	41985.0
61	39090.0
62	35345.5
63	31601.0
64	27636.5
65	23672.0
66	19843.5
67	16015.0
68	12830.5
69	9646.0
70	8048.0
71	6450.0
72	5019.0
73	3588.0
74	2868.5
75	1726.5
76	1304.0
77	978.0
78	652.0
79	432.0
80	212.0
81	222.0
82	232.0
83	138.0
84	44.0
85	37.0
86	30.0
87	19.5
88	9.0
89	8.0
90	7.0
91	4.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	966950.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.80007470209633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.13320826247049	22.525082776880595
2	9.265353169987863	5.707471395538624
3	3.246695426944257	2.99995385054513
4	1.7307088388034575	2.132238460908995
5	1.184591354003898	1.8242751097388747
6	0.9206313850931809	1.7013309260378628
7	0.7698408634780913	1.6597809272696087
8	0.6959648751724187	1.7148616116276514
9	0.6131715382330167	1.699715626449861
>10	8.299462216436766	49.5580529690562
>50	0.08442670371716623	1.7625975988566496
>100	0.04848598357149846	2.747073304233192
>500	0.004407816688312031	0.9710050402106288
>1k	0.0027125025774227883	2.351068667483533
>5k	3.3906282217784854E-4	0.6454917351626045
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC	6181	0.6392264336315218	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4931	0.5099539790061534	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGC	4715	0.48761569884688977	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCG	4257	0.44025027147215473	No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	3118	0.322457210817519	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTT	1683	0.17405243290759606	No Hit
GAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT	1491	0.15419618387713946	No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT	1177	0.12172294327524691	No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	1141	0.1179998965820363	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0341796370029474E-4	0.0	0.0	0.1258596618232587	0.0
2	1.0341796370029474E-4	0.0	0.0	0.6878328765706603	0.0
3	1.0341796370029474E-4	0.0	0.0	0.9524794456797145	0.0
4	1.0341796370029474E-4	0.0	0.0	1.4561249289001499	0.0
5	1.0341796370029474E-4	0.0	0.0	3.0285950669631316	0.0
6	1.0341796370029474E-4	0.0	0.0	3.9830394539531517	0.0
7	1.0341796370029474E-4	0.0	0.0	4.657221159315373	0.0
8	1.0341796370029474E-4	0.0	0.0	5.541755002843994	0.0
9	1.0341796370029474E-4	0.0	0.0	5.903924711722426	0.0
10	1.0341796370029474E-4	0.0	0.0	7.020631883758209	0.0
11	1.0341796370029474E-4	0.0	0.0	8.082320699105434	0.0
12	1.0341796370029474E-4	0.0	0.0	9.311443197683438	0.0
13	1.0341796370029474E-4	0.0	0.0	9.676612027509178	0.0
14	1.0341796370029474E-4	0.0	0.0	9.859248151403898	0.0
15	1.0341796370029474E-4	0.0	0.0	10.209317958529397	0.0
16	1.0341796370029474E-4	0.0	0.0	10.722271058482859	0.0
17	1.0341796370029474E-4	0.0	0.0	11.286933140286468	0.0
18	1.0341796370029474E-4	0.0	0.0	11.884999224365272	0.0
19	1.0341796370029474E-4	0.0	0.0	12.384921660892497	0.0
20	1.0341796370029474E-4	0.0	0.0	12.825999276074254	0.0
21	1.0341796370029474E-4	0.0	0.0	13.376389678887223	0.0
22	1.0341796370029474E-4	0.0	0.0	13.928745023010498	0.0
23	1.0341796370029474E-4	0.0	0.0	14.427323026009617	0.0
24	1.0341796370029474E-4	0.0	0.0	14.863333160970061	0.0
25	1.0341796370029474E-4	0.0	0.0	15.286209214540566	0.0
26	1.0341796370029474E-4	0.0	0.0	15.682610269403796	0.0
27	1.0341796370029474E-4	0.0	0.0	16.078390816484823	0.0
28	1.0341796370029474E-4	0.0	0.0	16.4570039815916	0.0
29	1.0341796370029474E-4	0.0	0.0	16.857748590930246	0.0
30	1.0341796370029474E-4	0.0	0.0	17.36522053880759	0.0
31	1.0341796370029474E-4	0.0	0.0	17.78602823310409	0.0
32	1.0341796370029474E-4	0.0	0.0	18.212317079476705	0.0
33	1.0341796370029474E-4	0.0	0.0	18.64553492941724	0.0
34	1.0341796370029474E-4	0.0	0.0	19.071306685971354	0.0
35	1.0341796370029474E-4	0.0	0.0	19.520761156212835	0.0
36	1.0341796370029474E-4	0.0	0.0	19.945085061275144	0.0
37	1.0341796370029474E-4	0.0	0.0	20.382853301618493	0.0
38	2.0683592740058948E-4	0.0	0.0	20.87367495734009	0.0
39	2.0683592740058948E-4	0.0	0.0	21.55137287346812	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACC	20	7.03273E-4	45.000004	13
CGTACTC	20	7.03273E-4	45.000004	40
TATCGCG	25	3.8903458E-5	45.0	32
TATAGCG	30	2.165143E-6	44.999996	1
CGTTTTT	2465	0.0	40.801216	1
ATAACGG	50	1.0804797E-9	40.5	2
CGGTCTA	100	0.0	38.25	31
GTTAGCG	65	9.094947E-12	38.07692	1
TATTACG	30	1.139788E-4	37.499996	1
TACGGCT	525	0.0	37.285717	7
CGAATAT	115	0.0	37.173912	14
TTACACG	540	0.0	37.083332	34
ACAACGA	280	0.0	36.964283	13
TCACGAC	105	0.0	36.42857	25
CTATACG	25	0.0021069439	36.0	1
CGGTTAC	25	0.0021069439	36.0	45
CCTCGCA	25	0.0021069439	36.0	43
CTACGAA	125	0.0	36.0	11
TTGCGCA	25	0.0021069439	36.0	19
CTACCGT	25	0.0021069439	36.0	20
>>END_MODULE