##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551701_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	454670
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.304425187498627	31.0	31.0	33.0	28.0	34.0
2	31.30088415774078	31.0	31.0	34.0	28.0	34.0
3	31.161789869575735	31.0	31.0	34.0	28.0	34.0
4	35.01429828227066	35.0	35.0	37.0	32.0	37.0
5	35.29626322387666	35.0	35.0	37.0	33.0	37.0
6	35.22408120175072	37.0	35.0	37.0	33.0	37.0
7	35.611687597598255	37.0	35.0	37.0	35.0	37.0
8	35.04786768425451	37.0	35.0	37.0	32.0	37.0
9	37.023067279565396	39.0	37.0	39.0	32.0	39.0
10	36.55440209382629	38.0	35.0	39.0	32.0	39.0
11	36.80031011502848	39.0	37.0	39.0	33.0	39.0
12	36.90315613521895	39.0	37.0	39.0	33.0	39.0
13	36.9652539204258	39.0	37.0	39.0	33.0	39.0
14	37.979785338817166	40.0	37.0	41.0	33.0	41.0
15	38.10906371654167	40.0	37.0	41.0	33.0	41.0
16	38.13866980447357	40.0	37.0	41.0	34.0	41.0
17	37.97973695207513	40.0	37.0	41.0	33.0	41.0
18	37.980757472452545	39.0	37.0	41.0	33.0	41.0
19	37.93404007301999	39.0	37.0	41.0	34.0	41.0
20	37.93712582752326	40.0	37.0	41.0	34.0	41.0
21	37.72483339564959	39.0	36.0	41.0	33.0	41.0
22	37.86193283040447	40.0	36.0	41.0	33.0	41.0
23	37.93917346647019	40.0	37.0	41.0	34.0	41.0
24	37.844656564101435	40.0	36.0	41.0	33.0	41.0
25	37.42280775067631	39.0	36.0	41.0	32.0	41.0
26	37.592423076077154	39.0	36.0	41.0	33.0	41.0
27	37.69696923043086	40.0	36.0	41.0	33.0	41.0
28	37.60182769921042	40.0	36.0	41.0	33.0	41.0
29	37.68404557151341	40.0	36.0	41.0	33.0	41.0
30	37.3898849715178	39.0	36.0	41.0	33.0	41.0
31	37.44132667649064	39.0	36.0	41.0	33.0	41.0
32	37.36185585149669	39.0	36.0	41.0	33.0	41.0
33	37.42008269734093	40.0	36.0	41.0	33.0	41.0
34	37.334882442210834	40.0	36.0	41.0	33.0	41.0
35	37.300908351111794	40.0	36.0	41.0	33.0	41.0
36	37.125513009435416	40.0	35.0	41.0	32.0	41.0
37	37.156834627312115	40.0	35.0	41.0	32.0	41.0
38	37.02922999098247	39.0	35.0	41.0	32.0	41.0
39	37.00241273890954	39.0	35.0	41.0	32.0	41.0
40	36.911964721666266	39.0	35.0	41.0	31.0	41.0
41	36.71934589922361	39.0	35.0	41.0	31.0	41.0
42	36.81240680053665	39.0	35.0	41.0	31.0	41.0
43	36.821474915873054	39.0	35.0	41.0	31.0	41.0
44	36.88449424857589	39.0	35.0	41.0	32.0	41.0
45	36.854250335408096	39.0	35.0	41.0	32.0	41.0
46	36.77369300811578	39.0	35.0	41.0	31.0	41.0
47	36.61822640596476	39.0	35.0	41.0	31.0	41.0
48	36.5657333890514	39.0	35.0	41.0	31.0	41.0
49	36.645094244177095	39.0	35.0	41.0	31.0	41.0
50	36.577849869135854	39.0	35.0	41.0	31.0	41.0
51	35.722937515120854	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	12.0
9	20.0
10	27.0
11	29.0
12	33.0
13	25.0
14	29.0
15	35.0
16	59.0
17	71.0
18	130.0
19	214.0
20	399.0
21	773.0
22	1131.0
23	1590.0
24	2192.0
25	2788.0
26	3594.0
27	3959.0
28	4561.0
29	5290.0
30	6544.0
31	8523.0
32	11259.0
33	16397.0
34	29304.0
35	32523.0
36	36927.0
37	57646.0
38	105756.0
39	122812.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.08397299140036	20.571403435458684	21.39617744737942	11.948446125761542
2	32.732311346691006	26.481404095277895	22.932236567180592	17.854047990850507
3	33.63230474849891	25.811907537334772	24.620713924384717	15.935073789781601
4	29.793476587415046	24.64578705434711	26.293795500032992	19.26694085820485
5	28.15052675566895	29.556821430927926	22.850858864671082	19.441792948732047
6	26.701563771526605	36.2614643587657	22.700640024633252	14.336331845074449
7	75.57921129610486	7.385136472606506	12.110101832098005	4.925550399190621
8	74.70956957793564	9.680207623111267	9.962610244792927	5.64761255416016
9	68.08542459366134	9.989882772120438	12.105263157894736	9.819429476323489
10	40.99676688587327	25.177161457760572	19.380869641718167	14.445202014647988
11	31.23100270525876	25.912200057184332	24.024457298700156	18.832339938856755
12	28.39421998372446	23.934282006730157	26.92392284514043	20.747575164404953
13	26.29797435502672	24.218884025776937	29.29267380737678	20.190467811819563
14	20.865242923438977	28.847075901203073	27.700749994501507	22.586931180856446
15	20.478148987177512	27.533595794752237	32.08216948556096	19.906085732509293
16	22.67468713572481	26.656256185805088	29.37295181120373	21.29610486726637
17	22.33488024281347	28.111157542833265	27.427144962280337	22.126817252072932
18	21.692436272461347	28.53234213825412	27.80478148987178	21.970440099412762
19	24.241097939164668	28.13293157674797	26.213957375679065	21.412013108408296
20	26.14533617788726	27.522158928453607	28.199573316911163	18.13293157674797
21	25.162425495414258	28.458882266259046	27.162557459256163	19.216134779070533
22	23.133041546616226	25.279653374975258	28.88028680141641	22.707018276992105
23	23.79110123826072	27.60199705280753	27.95631996832868	20.650581740603073
24	22.898585787494227	26.75171003145138	28.12523368597005	22.224470495084347
25	22.37007060065542	28.99465546440275	26.608089383508922	22.027184551432907
26	22.066333824532077	28.52178503090153	27.933446235731406	21.478434908834977
27	24.077242835463082	28.30602414938307	27.41240899993402	20.20432401521983
28	20.24545274594761	28.925814326874438	29.05579871115314	21.772934216024808
29	22.446829568698178	27.58176259704841	28.27391294785229	21.697494886401124
30	22.842281214947104	27.463215079068338	27.48674863087514	22.20775507510942
31	22.903644401434008	27.873842567136602	25.364770053005476	23.857742978423914
32	23.213319550443178	30.43394110013856	25.742406580596917	20.610332768821344
33	23.070578661446763	28.245540721842215	24.80238414674379	23.881496469967228
34	21.152264279587392	28.81826379571997	27.528097301339432	22.5013746233532
35	23.61009083511118	27.566146875756043	26.24980755273055	22.57395473640223
36	23.050784085160668	29.157190929685267	25.535883168011964	22.2561418171421
37	22.719114962500274	29.990982470803	26.317548991576306	20.972353575120415
38	21.554094178195175	28.293707524138384	24.927309917082717	25.22488838058372
39	23.467569885851276	27.74517782127697	26.499219213935383	22.28803307893637
40	22.30694789627642	26.581916554863966	28.95836540787824	22.15277014098137
41	20.845668286889392	28.710713264565506	28.02362152770141	22.419996920843687
42	21.842435172762663	27.93608551256956	27.779928299645896	22.441551015021886
43	23.89095387863725	26.344381639430797	26.945476939318624	22.819187542613324
44	22.265379286075614	27.191149625002748	26.881474475993578	23.661996612928057
45	22.041040754833176	26.0432841401456	26.07429564299382	25.841379462027405
46	22.317505003629005	28.6997162777399	26.65735588448765	22.325422834143442
47	21.029537906613587	27.04664921811424	29.65953328787912	22.264279587393055
48	21.0477929047441	26.896210438339896	27.733521015241823	24.32247564167418
49	20.800800580640903	26.268282490597578	30.032771020740316	22.898145908021203
50	20.863263465810366	26.213297556469527	28.42655112499175	24.496887852728353
51	20.609672949611806	25.731849473244335	27.225680163635165	26.432797413508695
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	100.0
1	492.0
2	884.0
3	1767.0
4	2650.0
5	1927.5
6	1205.0
7	1149.0
8	1093.0
9	1107.5
10	1122.0
11	1113.0
12	1104.0
13	1049.0
14	994.0
15	984.0
16	974.0
17	1025.5
18	1077.0
19	1048.0
20	1019.0
21	1700.0
22	2381.0
23	2251.0
24	2121.0
25	2590.0
26	3767.5
27	4476.0
28	5662.0
29	6848.0
30	8127.0
31	9406.0
32	10154.5
33	10903.0
34	11698.5
35	12494.0
36	13661.0
37	14828.0
38	16643.5
39	18459.0
40	20162.5
41	21866.0
42	22854.5
43	23843.0
44	25776.0
45	27709.0
46	35761.0
47	43813.0
48	42091.5
49	40370.0
50	39472.5
51	38575.0
52	34611.0
53	30647.0
54	27615.5
55	24584.0
56	23072.5
57	21561.0
58	21093.5
59	20626.0
60	20055.0
61	19484.0
62	17595.0
63	15706.0
64	12955.0
65	10204.0
66	8398.0
67	6592.0
68	5591.0
69	4590.0
70	3702.5
71	2815.0
72	2447.0
73	2079.0
74	1598.0
75	896.0
76	675.0
77	506.5
78	338.0
79	235.5
80	133.0
81	102.5
82	72.0
83	70.5
84	69.0
85	35.5
86	2.0
87	2.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	454670.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.664944991203
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.00463483145909	22.386831112095557
2	8.800935414640648	5.397604007221717
3	3.0915071488556625	2.8440268997870914
4	1.6925800607551496	2.0761149782505477
5	1.1588525990253977	1.7768075601013227
6	0.9570465191948577	1.7608667319079574
7	0.885707193894532	1.9012113665362016
8	0.7743002998703775	1.8995100881757692
9	0.69133039722329	1.9079647741438839
>10	8.806295382467418	50.2491615459344
>50	0.08064598469855802	1.6827472054374797
>100	0.0482435801321731	2.784338310007429
>500	0.0043203206088513225	0.7959983828371754
>1k	0.0036002671740427684	2.5368170375634698
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGC	3021	0.664437944003343	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTC	2734	0.601315239624343	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCG	2570	0.5652451228363429	No Hit
GCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	1811	0.39831086282358635	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1353	0.2975784635010007	No Hit
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT	769	0.16913365737787844	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACAAGT	643	0.1414212505773418	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCT	577	0.1269052279675369	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	543	0.11942727692612223	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	538	0.11832757824356127	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	535	0.11766775903402468	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCT	495	0.10887016957353685	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	464	0.10205203774165877	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTA	458	0.1007323993225856	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.398794730243913E-4	0.0	0.0	0.17859106604790287	0.0
2	4.398794730243913E-4	0.0	0.0	0.8540259968768558	0.0
3	4.398794730243913E-4	0.0	0.0	1.0920007917830514	0.0
4	4.398794730243913E-4	0.0	0.0	1.5375986979567597	0.0
5	4.398794730243913E-4	0.0	0.0	3.0254910154617636	0.0
6	4.398794730243913E-4	0.0	0.0	3.5762201156883013	0.0
7	4.398794730243913E-4	0.0	0.0	4.175995777157059	0.0
8	4.398794730243913E-4	0.0	0.0	4.948864011260914	0.0
9	4.398794730243913E-4	0.0	0.0	5.189258143268744	0.0
10	4.398794730243913E-4	0.0	0.0	6.32876591813843	0.0
11	4.398794730243913E-4	0.0	0.0	7.268788351991554	0.0
12	4.398794730243913E-4	0.0	0.0	8.662106582796314	0.0
13	4.398794730243913E-4	0.0	0.0	9.007411969120461	0.0
14	4.398794730243913E-4	0.0	0.0	9.17962478280951	0.0
15	4.398794730243913E-4	0.0	0.0	9.565179140915388	0.0
16	4.398794730243913E-4	0.0	0.0	10.10029251984956	0.0
17	4.398794730243913E-4	0.0	0.0	10.696329205797612	0.0
18	4.398794730243913E-4	0.0	0.0	11.27828974860888	0.0
19	4.398794730243913E-4	0.0	0.0	11.836716739613346	0.0
20	4.398794730243913E-4	0.0	0.0	12.268678382123298	0.0
21	4.398794730243913E-4	0.0	0.0	12.772340378736226	0.0
22	4.398794730243913E-4	0.0	0.0	13.354960740757033	0.0
23	4.398794730243913E-4	0.0	0.0	13.849385268436448	0.0
24	4.398794730243913E-4	0.0	0.0	14.275628477797083	0.0
25	4.398794730243913E-4	0.0	0.0	14.663602173004596	0.0
26	4.398794730243913E-4	0.0	0.0	15.025842919040183	0.0
27	4.398794730243913E-4	0.0	0.0	15.400400290320452	0.0
28	4.398794730243913E-4	0.0	0.0	15.756262783997185	0.0
29	4.398794730243913E-4	0.0	0.0	16.118943409505796	0.0
30	4.398794730243913E-4	0.0	0.0	16.505377526557723	0.0
31	4.398794730243913E-4	0.0	0.0	16.91952405041019	0.0
32	4.398794730243913E-4	0.0	0.0	17.30551828798909	0.0
33	4.398794730243913E-4	0.0	0.0	17.706248487914312	0.0
34	4.398794730243913E-4	0.0	0.0	18.071568390261067	0.0
35	4.398794730243913E-4	0.0	0.0	18.445905821804825	0.0
36	6.598192095365869E-4	0.0	0.0	18.845536323047487	0.0
37	6.598192095365869E-4	0.0	0.0	19.238128752721753	0.0
38	8.797589460487826E-4	0.0	0.0	19.621043833989486	0.0
39	8.797589460487826E-4	0.0	0.0	20.00813777025095	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGTT	20	7.028782E-4	45.000004	4
GCGGTAA	20	7.028782E-4	45.000004	41
CGGCATG	20	7.028782E-4	45.000004	2
TATTGCG	20	7.028782E-4	45.000004	1
CATCGGT	20	7.028782E-4	45.000004	12
ATCGGTC	20	7.028782E-4	45.000004	13
TATACGG	30	2.1625965E-6	45.000004	2
CAATCCG	20	7.028782E-4	45.000004	1
CTGGACG	20	7.028782E-4	45.000004	41
GCGAATG	35	1.2097553E-7	45.0	1
ACGGGAA	70	0.0	41.785713	5
AACGGGT	50	1.0786607E-9	40.5	4
CGTTTTT	750	0.0	39.899998	1
CGATGAA	255	0.0	39.705883	19
TTGCGGG	165	0.0	39.545452	3
TTGGACG	40	3.452078E-7	39.375004	1
CCGATGA	270	0.0	39.166664	18
TACGGCT	355	0.0	38.661972	7
GGCCGAT	35	6.2399613E-6	38.571426	8
CGAACAC	30	1.1388367E-4	37.500004	34
>>END_MODULE