FastQCFastQC Report
Sat 18 Jun 2016
SRR3551696_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551696_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541887
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45340.8367058076683885No Hit
GCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC11130.20539337537161806No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9540.17605146460424406No Hit
CCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC8740.16128823906091122No Hit
CTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGCT7360.13582167499866207No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGA207.029981E-445.00000417
ACGGGTA207.029981E-445.0000045
TGGCACG253.8880673E-545.01
TATAGCG253.8880673E-545.01
TAGTCGG253.8880673E-545.02
ACACGAC850.042.3529426
CGTTTTA4550.042.032971
CGTTTTT21950.041.8223271
AGGCGAT1400.041.7857177
GCGTACG451.9250365E-840.01
GACCGTT356.242177E-638.571439
GCAAACG356.242177E-638.571431
TAGGCGA356.242177E-638.571436
CACGACC950.037.89473727
CAAACGG601.546141E-1037.5000042
CTCGTAC301.1391256E-437.50000429
CGTGCTA301.1391256E-437.50000438
GTCTACG301.1391256E-437.5000041
TACGGGT301.1391256E-437.5000044
TAACGCC301.1391256E-437.50000412