##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551696_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	541887
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.879676021015452	33.0	31.0	34.0	30.0	34.0
2	31.96068922118449	33.0	31.0	34.0	30.0	34.0
3	31.676183410932538	33.0	31.0	34.0	30.0	34.0
4	35.60015095398118	37.0	35.0	37.0	33.0	37.0
5	35.65575295218376	37.0	35.0	37.0	35.0	37.0
6	35.632499026549816	37.0	35.0	37.0	33.0	37.0
7	30.06322166798613	37.0	35.0	37.0	0.0	37.0
8	31.99211643755986	37.0	33.0	37.0	17.0	37.0
9	36.02634313057889	39.0	34.0	39.0	32.0	39.0
10	36.38115511167457	38.0	35.0	39.0	32.0	39.0
11	36.71253231762341	39.0	35.0	39.0	33.0	39.0
12	37.07477758278017	39.0	37.0	39.0	34.0	39.0
13	37.16496243681801	39.0	37.0	39.0	34.0	39.0
14	38.22684987829566	40.0	38.0	41.0	33.0	41.0
15	38.39859601725083	40.0	38.0	41.0	34.0	41.0
16	38.25001153376996	40.0	37.0	41.0	34.0	41.0
17	38.129174532697775	40.0	37.0	41.0	33.0	41.0
18	37.98928374365873	39.0	37.0	41.0	33.0	41.0
19	37.82529014351701	39.0	37.0	41.0	33.0	41.0
20	37.886791895727335	39.0	36.0	41.0	34.0	41.0
21	37.82645090212535	39.0	36.0	41.0	33.0	41.0
22	37.93777669514124	39.0	36.0	41.0	34.0	41.0
23	37.89507591066034	40.0	36.0	41.0	34.0	41.0
24	37.84298017852431	40.0	36.0	41.0	34.0	41.0
25	37.48271134018716	39.0	35.0	41.0	33.0	41.0
26	37.549042512553356	39.0	36.0	41.0	33.0	41.0
27	37.60284708804603	39.0	36.0	41.0	33.0	41.0
28	37.52207194488888	39.0	36.0	41.0	33.0	41.0
29	37.52817838405424	39.0	36.0	41.0	33.0	41.0
30	37.3568400792047	39.0	36.0	41.0	33.0	41.0
31	37.227779961504886	39.0	35.0	41.0	32.0	41.0
32	37.150514775220664	39.0	35.0	41.0	32.0	41.0
33	37.03701325184033	39.0	35.0	41.0	32.0	41.0
34	36.92132123487	40.0	35.0	41.0	31.0	41.0
35	36.85478891355578	40.0	35.0	41.0	31.0	41.0
36	36.71960205725548	40.0	35.0	41.0	31.0	41.0
37	36.61471856678607	39.0	35.0	41.0	30.0	41.0
38	36.485854061824696	39.0	35.0	41.0	30.0	41.0
39	36.39924744457793	39.0	35.0	41.0	30.0	41.0
40	36.34164872012062	39.0	35.0	41.0	30.0	41.0
41	36.152823374615004	39.0	35.0	41.0	29.0	41.0
42	36.088826637287845	39.0	35.0	41.0	29.0	41.0
43	36.013506505968955	39.0	35.0	41.0	29.0	41.0
44	35.9288763155418	39.0	35.0	40.0	28.0	41.0
45	35.868003845820255	39.0	35.0	40.0	28.0	41.0
46	35.72997506860286	39.0	35.0	40.0	27.0	41.0
47	35.65876649559779	39.0	35.0	40.0	27.0	41.0
48	35.606408716208364	39.0	35.0	40.0	27.0	41.0
49	35.486167780367495	39.0	35.0	40.0	26.0	41.0
50	35.325866832014796	38.0	35.0	40.0	26.0	41.0
51	33.494841175374205	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	23.0
10	37.0
11	24.0
12	14.0
13	15.0
14	15.0
15	25.0
16	41.0
17	85.0
18	170.0
19	294.0
20	521.0
21	798.0
22	1299.0
23	2072.0
24	3076.0
25	4736.0
26	6519.0
27	7412.0
28	7647.0
29	8067.0
30	9136.0
31	11421.0
32	15043.0
33	21159.0
34	33746.0
35	43175.0
36	53447.0
37	84333.0
38	115064.0
39	112451.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.80368600833753	20.901405643612044	24.29602481698214	14.998883531068286
2	35.435063029746054	23.016975863971638	24.429078387191424	17.11888271909088
3	31.40562515801265	23.003319880344055	29.543982416998375	16.047072544644916
4	28.389498179509747	24.33846909041922	28.407767671119625	18.864265058951403
5	23.819172631932485	29.062332183647143	26.953774495420628	20.164720688999736
6	24.937856047478533	33.3386850025928	26.99197434151401	14.731484608414668
7	65.36824098013054	21.212540621937784	9.691688488559423	3.727529909372249
8	78.29344494331843	6.259054009415248	10.956343296665171	4.49115775060114
9	72.87515662859599	7.805870965717945	13.709500320177453	5.609472085508602
10	35.601702938066424	31.012185197282825	20.17320954368715	13.212902320963597
11	25.86996920022071	25.02182189275624	31.18473039582053	17.92347851120252
12	24.50437083746242	22.583121573316948	34.240718083290425	18.671789505930203
13	22.488452389520326	23.112014128406845	34.924624506585324	19.47490897548751
14	19.465312878884344	26.931076036147754	33.07922131366872	20.52438977129918
15	18.7581543753587	26.448872181838652	35.63215209074955	19.160821352053105
16	21.708215919555187	25.63228126897305	33.635794916652365	19.0237078948194
17	21.785907393976974	23.949827177990983	31.752560958280966	22.511704469751074
18	21.368108111100653	25.194551631613233	33.08641838612109	20.35092187116502
19	22.114204621996837	26.542065043080935	30.872488175579043	20.471242159343184
20	23.617839143585286	26.221149427832742	30.955531319260288	19.205480109321684
21	23.163131796850635	26.180735097907863	31.903884020100133	18.75224908514137
22	22.018612736603757	24.613803246802377	30.92157590051062	22.446008116083245
23	20.434887716442727	26.134230937446368	31.99726142166171	21.433619924449193
24	21.37899598993886	24.75626837329554	32.44329537338965	21.421440263375942
25	20.772411960427174	26.208785226440202	30.902383707304292	22.116419105828335
26	19.780876824873083	26.494638181022978	31.924552535860794	21.79993245824314
27	20.728306824116466	26.049896011530077	31.644051250537476	21.577745913815978
28	18.808533882525325	27.266201255981414	32.3316484802182	21.593616381275062
29	20.07189690839603	25.905400941524707	31.49457359191123	22.52812855816803
30	20.86763476518167	24.671564366740668	31.31999844986132	23.140802418216346
31	21.084469640349372	26.728450765565515	29.686078462852954	22.501001131232158
32	20.797324903531546	26.564025341076647	29.96897877232707	22.669670983064734
33	20.19738432551436	25.687643364760547	29.71320588978883	24.401766419936262
34	18.962255968495274	25.925515836327502	32.037491211267294	23.07473698390993
35	19.142182779804646	24.344743461275137	30.763978467835546	25.74909529108467
36	19.746921406123416	26.696156209689477	30.200576873038106	23.356345511149005
37	19.758362905919498	27.20585657157304	30.41814990948297	22.617630613024488
38	19.58507954610463	27.082768178605505	28.785521704709655	24.546630570580213
39	20.470319457746726	24.63299544000871	30.3467328059171	24.54995229632746
40	20.873540055399005	23.664896925004662	32.09506779088629	23.366495228710043
41	18.757969835039408	25.16447155956869	31.38551026320986	24.69204834218204
42	19.65280584328467	25.400314087623432	30.99428478631154	23.952595282780358
43	20.117109286622487	25.154690922646232	30.6521470343448	24.076052756386478
44	20.0394178122007	26.005975415538664	29.56741903754842	24.387187734712217
45	20.452788127414017	24.04080555540177	29.436949031809217	26.069457285374998
46	20.22838709915536	25.093608076960695	29.675559664653335	25.002445159230614
47	19.34609983262193	25.046365755222027	32.16906845892225	23.43846595323379
48	19.124466909152645	24.520425845240798	31.254302096193488	25.10080514941307
49	19.93275350765012	24.170168319225226	32.00205946996329	23.895018703161362
50	19.16137497301098	23.446401186963335	32.10392572621229	25.288298113813397
51	18.591699007357622	23.354500107956085	30.50100851284493	27.552792371841363
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	171.0
1	540.0
2	909.0
3	5017.0
4	9125.0
5	6096.5
6	3068.0
7	2828.5
8	2589.0
9	2540.5
10	2492.0
11	2442.5
12	2393.0
13	2379.5
14	2366.0
15	2203.5
16	2041.0
17	1982.5
18	1924.0
19	1998.5
20	2073.0
21	2354.5
22	2636.0
23	2669.5
24	2703.0
25	3412.5
26	4816.5
27	5511.0
28	6425.5
29	7340.0
30	8870.5
31	10401.0
32	12090.5
33	13780.0
34	14909.5
35	16039.0
36	17828.0
37	19617.0
38	21197.5
39	22778.0
40	25228.0
41	27678.0
42	29581.0
43	31484.0
44	34553.0
45	37622.0
46	42135.5
47	46649.0
48	49747.0
49	52845.0
50	50929.5
51	49014.0
52	42769.5
53	36525.0
54	32279.0
55	28033.0
56	25650.0
57	23267.0
58	21755.0
59	20243.0
60	18824.0
61	17405.0
62	15308.0
63	13211.0
64	10874.0
65	8537.0
66	6916.0
67	5295.0
68	4492.5
69	3690.0
70	2981.0
71	2272.0
72	1975.0
73	1678.0
74	1358.5
75	819.5
76	600.0
77	499.0
78	398.0
79	285.0
80	172.0
81	120.5
82	69.0
83	56.5
84	44.0
85	32.0
86	20.0
87	12.0
88	4.0
89	6.5
90	9.0
91	6.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	541887.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.76523390975366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.68682785143983	33.72294912688895
2	9.943394208118455	9.101235235828618
3	4.178646041487264	5.7371014054419245
4	2.5190050668950947	4.6113142442523465
5	1.7525709465207824	4.010340965548099
6	1.2890097805107972	3.539510047022211
7	1.0454010147949166	3.3490115380108243
8	0.9159813205586408	3.3536079513864996
9	0.7591490466180527	3.12683703217575
>10	3.8547019423134405	25.44884361713863
>50	0.036073552694926375	1.0980416280536638
>100	0.017235141792847873	1.3950228135280784
>500	0.0012024517529893863	0.4703272181309655
>1k	8.016345019929243E-4	1.035857176593433
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4534	0.8367058076683885	No Hit
GCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC	1113	0.20539337537161806	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	954	0.17605146460424406	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC	874	0.16128823906091122	No Hit
CTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGCT	736	0.13582167499866207	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17642054524282738	0.0
2	0.0	0.0	0.0	0.8001668244486396	0.0
3	0.0	0.0	0.0	1.1400900925838782	0.0
4	0.0	0.0	0.0	1.472816288266742	0.0
5	0.0	0.0	0.0	2.423198932618793	0.0
6	0.0	0.0	0.0	3.0687209695010216	0.0
7	0.0	0.0	0.0	3.668292466879626	0.0
8	0.0	0.0	0.0	4.822592164048962	0.0
9	0.0	0.0	0.0	5.277114970464322	0.0
10	0.0	0.0	0.0	6.221961405237623	0.0
11	0.0	0.0	0.0	7.544377333281662	0.0
12	0.0	0.0	0.0	8.632611596144583	0.0
13	0.0	0.0	0.0	9.083997217131985	0.0
14	0.0	0.0	0.0	9.269829318658687	0.0
15	0.0	0.0	0.0	9.60347821593801	0.0
16	0.0	0.0	0.0	10.296427114878194	0.0
17	0.0	0.0	0.0	11.108589060080792	0.0
18	0.0	0.0	0.0	12.005455011838261	0.0
19	0.0	0.0	0.0	12.548372631194326	0.0
20	0.0	0.0	0.0	13.079295129796433	0.0
21	0.0	0.0	0.0	13.705440433153038	0.0
22	1.8454031929166043E-4	0.0	0.0	14.374583630904597	0.0
23	1.8454031929166043E-4	0.0	0.0	15.043173207698285	0.0
24	1.8454031929166043E-4	0.0	0.0	15.558040698522017	0.0
25	1.8454031929166043E-4	0.0	0.0	16.022528682179125	0.0
26	1.8454031929166043E-4	0.0	0.0	16.489046609348442	0.0
27	1.8454031929166043E-4	0.0	0.0	16.920686416171637	0.0
28	1.8454031929166043E-4	0.0	0.0	17.390526069088203	0.0
29	1.8454031929166043E-4	0.0	0.0	17.87918883457252	0.0
30	1.8454031929166043E-4	0.0	0.0	18.412325817006128	0.0
31	3.6908063858332086E-4	0.0	0.0	18.942879234969652	0.0
32	3.6908063858332086E-4	0.0	0.0	19.432095621411843	0.0
33	3.6908063858332086E-4	0.0	0.0	19.896952685707536	0.0
34	3.6908063858332086E-4	0.0	0.0	20.369375903094188	0.0
35	3.6908063858332086E-4	0.0	0.0	20.868557466778128	0.0
36	3.6908063858332086E-4	0.0	0.0	21.36663178854632	0.0
37	3.6908063858332086E-4	0.0	0.0	21.840900409125886	0.0
38	3.6908063858332086E-4	0.0	0.0	22.31461540874758	0.0
39	3.6908063858332086E-4	0.0	0.0	22.830774681806353	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGGA	20	7.029981E-4	45.000004	17
ACGGGTA	20	7.029981E-4	45.000004	5
TGGCACG	25	3.8880673E-5	45.0	1
TATAGCG	25	3.8880673E-5	45.0	1
TAGTCGG	25	3.8880673E-5	45.0	2
ACACGAC	85	0.0	42.35294	26
CGTTTTA	455	0.0	42.03297	1
CGTTTTT	2195	0.0	41.822327	1
AGGCGAT	140	0.0	41.785717	7
GCGTACG	45	1.9250365E-8	40.0	1
GACCGTT	35	6.242177E-6	38.57143	9
GCAAACG	35	6.242177E-6	38.57143	1
TAGGCGA	35	6.242177E-6	38.57143	6
CACGACC	95	0.0	37.894737	27
CAAACGG	60	1.546141E-10	37.500004	2
CTCGTAC	30	1.1391256E-4	37.500004	29
CGTGCTA	30	1.1391256E-4	37.500004	38
GTCTACG	30	1.1391256E-4	37.500004	1
TACGGGT	30	1.1391256E-4	37.500004	4
TAACGCC	30	1.1391256E-4	37.500004	12
>>END_MODULE