FastQCFastQC Report
Sat 18 Jun 2016
SRR3551695_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551695_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542906
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45200.8325566488489721No Hit
GCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC14250.26247637712605865No Hit
CCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC12280.2261901692005614No Hit
CTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGCT10510.19358784025227202No Hit
CGCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTG6140.1130950846002807No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAT600.045.00000414
GCATACG302.1633768E-645.0000041
GTTTACG253.8880775E-545.01
CGCATCG253.8880775E-545.021
ATTGTCG207.029994E-445.011
GTCAACG253.8880775E-545.01
GACGATG207.029994E-445.01
CGTTTTT22300.042.2757871
GTTAGCG650.041.538461
TCAACGG650.041.538462
CGGTCTA451.9250365E-840.00000431
CCCGATT900.040.00000442
CCGCGAG403.4537334E-739.37516
ATCGAGG403.4537334E-739.3752
CTCGATC700.038.5714316
AAGGGCG1350.038.3333325
CAAGGGC2350.038.297874
TCGATCA659.094947E-1238.0769217
CTACGAA659.094947E-1238.0769211
AGGGCGC659.094947E-1238.076926