##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551695_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	542906
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.323450100017315	31.0	31.0	34.0	28.0	34.0
2	31.39140477357038	31.0	31.0	34.0	28.0	34.0
3	31.328828563324038	31.0	31.0	34.0	28.0	34.0
4	35.20915407087046	37.0	35.0	37.0	32.0	37.0
5	32.88391360567022	37.0	35.0	37.0	28.0	37.0
6	34.00747827432373	37.0	35.0	37.0	28.0	37.0
7	35.28192725812572	37.0	35.0	37.0	32.0	37.0
8	35.1516413522783	37.0	35.0	37.0	33.0	37.0
9	37.115710638674095	39.0	37.0	39.0	33.0	39.0
10	36.34847284797	38.0	35.0	39.0	32.0	39.0
11	36.53220815389773	39.0	35.0	39.0	32.0	39.0
12	36.735060949777676	39.0	35.0	39.0	33.0	39.0
13	36.85856299248857	39.0	37.0	39.0	33.0	39.0
14	37.80835172202923	40.0	37.0	41.0	33.0	41.0
15	37.99449260092907	40.0	37.0	41.0	33.0	41.0
16	38.03653855363544	40.0	37.0	41.0	33.0	41.0
17	38.018318088214166	40.0	37.0	41.0	33.0	41.0
18	37.910686196137085	39.0	37.0	41.0	33.0	41.0
19	37.7855337756444	39.0	37.0	41.0	33.0	41.0
20	37.87841173241777	39.0	36.0	41.0	34.0	41.0
21	37.77420216391051	39.0	36.0	41.0	33.0	41.0
22	37.96184053961459	40.0	36.0	41.0	34.0	41.0
23	38.00876579002627	40.0	36.0	41.0	34.0	41.0
24	37.87521228352606	40.0	36.0	41.0	34.0	41.0
25	37.44329589284333	39.0	35.0	41.0	33.0	41.0
26	37.63408582701241	39.0	36.0	41.0	33.0	41.0
27	37.60868179758558	39.0	36.0	41.0	33.0	41.0
28	37.57515297307453	39.0	36.0	41.0	33.0	41.0
29	37.69554397998917	40.0	36.0	41.0	33.0	41.0
30	37.318408711637005	39.0	35.0	41.0	33.0	41.0
31	37.21249719104228	39.0	35.0	41.0	32.0	41.0
32	37.196918066847665	39.0	35.0	41.0	33.0	41.0
33	37.212318522911886	40.0	36.0	41.0	33.0	41.0
34	37.12875709607188	40.0	36.0	41.0	32.0	41.0
35	37.112693541791764	40.0	36.0	41.0	32.0	41.0
36	36.98101144581198	40.0	35.0	41.0	32.0	41.0
37	37.02688679071515	40.0	36.0	41.0	32.0	41.0
38	36.79646200263029	39.0	35.0	41.0	31.0	41.0
39	36.781976990491906	39.0	35.0	41.0	31.0	41.0
40	36.61137655505741	39.0	35.0	41.0	31.0	41.0
41	36.46147583559585	39.0	35.0	41.0	30.0	41.0
42	36.632323090921815	39.0	35.0	41.0	31.0	41.0
43	36.62024917757402	39.0	35.0	41.0	31.0	41.0
44	36.660292942056266	39.0	35.0	41.0	31.0	41.0
45	36.65052697888769	39.0	35.0	41.0	31.0	41.0
46	36.592953476292394	39.0	35.0	41.0	31.0	41.0
47	36.5542911664266	39.0	35.0	41.0	31.0	41.0
48	36.50581316102603	39.0	35.0	41.0	31.0	41.0
49	36.504148047728336	39.0	35.0	41.0	31.0	41.0
50	36.444443421144726	39.0	35.0	41.0	31.0	41.0
51	35.546807366284405	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	17.0
9	42.0
10	29.0
11	31.0
12	24.0
13	33.0
14	43.0
15	55.0
16	79.0
17	120.0
18	203.0
19	319.0
20	500.0
21	745.0
22	1134.0
23	1715.0
24	2616.0
25	4154.0
26	5595.0
27	6483.0
28	6503.0
29	6883.0
30	8082.0
31	10348.0
32	13888.0
33	20323.0
34	33780.0
35	39854.0
36	48288.0
37	73900.0
38	121912.0
39	135189.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.76213193444169	21.135334661985684	23.871167384409087	15.23136601916354
2	35.56950927048145	22.822367039598017	24.1789554729548	17.42916821696574
3	31.642310086829028	22.742426865792606	28.978497198410036	16.63676584896833
4	28.54822013387216	24.483059682523308	27.61675133448516	19.35196884911937
5	22.55289129241526	33.69717778031556	24.725090531325865	19.02484039594331
6	25.41360751216601	33.09928422231473	26.304553642803725	15.182554622715536
7	78.41025886617572	5.750166695523719	11.317244605880207	4.522329832420345
8	79.80958029566813	4.537617930175758	10.705352307765985	4.947449466390131
9	73.0818963135423	7.886816502304267	13.325879618202782	5.705407565950643
10	35.88577028067474	30.988237374425776	19.761800385333743	13.364191959565744
11	25.92677185369106	25.11760783634736	30.74565394377664	18.209966366184936
12	24.566315347408207	22.521946709006716	33.79608993085359	19.115648012731487
13	22.837102555506846	23.03584782632721	34.51978795592608	19.60726166223987
14	19.734908068800124	26.825085742283193	32.752815404508326	20.68719078440835
15	19.017104250091176	26.25961031928179	35.14549480020482	19.57779063042221
16	21.887030167284944	25.729868522359308	33.15767370410347	19.22542760625228
17	21.961076134726824	24.161272853864205	31.33231167089699	22.545339340511987
18	21.750174063281673	24.85457887737472	32.75649928348554	20.638747775858068
19	22.093695777906305	26.500536004391183	30.87532648377436	20.530441733928157
20	23.896770343300684	26.158672035306296	30.806622140849427	19.137935480543593
21	23.353582388111384	26.141726192011138	31.536582760183162	18.96810865969431
22	21.97710100827768	24.58989217286234	31.048837183600845	22.384169635259145
23	20.778919370940827	26.011869458064563	31.72372381222532	21.48548735876929
24	21.3863910142824	25.055166087683688	32.16026347102445	21.398179427009463
25	20.913565147557772	26.264951943798742	30.579879389802283	22.2416035188412
26	19.868632875672766	26.82361219069231	31.46769422330938	21.840060710325545
27	20.792365529207636	26.10304546275046	31.478745860241	21.625843147800907
28	19.091886993328494	27.560940567980463	31.76829874784954	21.578873690841508
29	20.27312278737019	25.773154100341493	31.329180373766363	22.624542738521956
30	21.012108910198084	24.992540145071153	30.924506268120076	23.070844676610683
31	21.229641963802205	26.56076742566853	29.380776782721135	22.82881382780813
32	21.036238317498793	26.797825037851858	29.522421929394778	22.643514715254575
33	20.420846334356224	25.686398750428253	29.355542211727258	24.537212703488265
34	19.23905795846795	25.943901890935077	31.608418400238712	23.208621750358258
35	19.33944366059686	24.411039848518897	30.55924966752992	25.690266823354317
36	20.03072355066991	26.76153882992636	29.77384666958921	23.43389094981452
37	20.087271092970056	27.07448434904017	30.123262590577376	22.714981967412406
38	19.766405234055252	27.014252927762815	28.517828132310196	24.701513705871736
39	20.557886632308357	24.585103130191968	30.255882233756857	24.60112800374282
40	21.052631578947366	23.529487609273062	31.87144735921136	23.546433452568216
41	18.84525129580443	24.988303684247366	31.370071430413365	24.796373589534838
42	19.72311965607306	25.29995984571915	30.79612308576439	24.180797412443404
43	20.206076189985005	25.139526916261744	30.403053198896306	24.251343694856935
44	20.092981105384723	25.9354289692875	29.488161854906743	24.48342807042103
45	20.56507019631391	23.91426876844242	29.403801026328686	26.116860008914987
46	20.305172534471897	25.011143733906056	29.665172239761578	25.01851149186047
47	19.323787174943728	25.204363186260604	31.814715622962353	23.65713401583331
48	19.21234983588319	24.60499607666889	31.170957771695285	25.011696315752634
49	20.161501254360793	24.0743333100021	31.89281385727916	23.871351578357945
50	19.29671066446125	23.41105090015583	31.605471297056948	25.686767138325973
51	18.91966565114403	23.39299989316751	30.320718503755717	27.36661595193275
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	135.0
1	470.5
2	806.0
3	4262.5
4	7719.0
5	5147.5
6	2576.0
7	2504.5
8	2433.0
9	2398.5
10	2364.0
11	2351.0
12	2338.0
13	2235.0
14	2132.0
15	2063.5
16	1995.0
17	1921.5
18	1848.0
19	1879.0
20	1910.0
21	2287.0
22	2664.0
23	2570.5
24	2477.0
25	3161.5
26	4618.0
27	5390.0
28	6062.0
29	6734.0
30	8320.0
31	9906.0
32	11659.0
33	13412.0
34	14627.0
35	15842.0
36	17485.5
37	19129.0
38	20667.5
39	22206.0
40	24605.5
41	27005.0
42	28777.5
43	30550.0
44	33636.0
45	36722.0
46	41038.5
47	45355.0
48	49083.5
49	52812.0
50	52020.0
51	51228.0
52	44659.5
53	38091.0
54	33469.5
55	28848.0
56	26624.0
57	24400.0
58	22711.0
59	21022.0
60	19622.5
61	18223.0
62	16238.5
63	14254.0
64	11977.5
65	9701.0
66	7730.5
67	5760.0
68	4913.0
69	4066.0
70	3252.0
71	2438.0
72	2173.0
73	1908.0
74	1566.5
75	983.5
76	742.0
77	585.0
78	428.0
79	294.5
80	161.0
81	114.0
82	67.0
83	47.0
84	27.0
85	16.5
86	6.0
87	4.0
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	542906.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.9751053055905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.60239343994087	30.158658219450814
2	9.166673526748735	7.512108261209963
3	3.53936577222981	4.350776556963573
4	2.144847325412043	3.5154138009269045
5	1.6413243671524378	3.3626719392351423
6	1.3592816606359766	3.3418025510710243
7	1.173296191014512	3.365315448702751
8	0.987969103737019	3.238571045143538
9	0.8740501649370994	3.223286779559975
>10	5.438314682242322	33.190485101860915
>50	0.04666142433123104	1.311639473464745
>100	0.023557223932144974	1.788700532897526
>500	4.5302353715663405E-4	0.11397489904515162
>1k	0.0018120941486265362	1.5265953904679592
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4520	0.8325566488489721	No Hit
GCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC	1425	0.26247637712605865	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC	1228	0.2261901692005614	No Hit
CTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGCT	1051	0.19358784025227202	No Hit
CGCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTG	614	0.1130950846002807	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8419394886039204E-4	0.0	0.0	0.20021882241124614	0.0
2	1.8419394886039204E-4	0.0	0.0	0.8741844812914206	0.0
3	1.8419394886039204E-4	0.0	0.0	1.2250739538704674	0.0
4	1.8419394886039204E-4	0.0	0.0	1.6151967375567777	0.0
5	1.8419394886039204E-4	0.0	0.0	2.6126069706358006	0.0
6	1.8419394886039204E-4	0.0	0.0	3.37351217337808	0.0
7	1.8419394886039204E-4	0.0	0.0	4.085605979672356	0.0
8	1.8419394886039204E-4	0.0	0.0	5.218582959112627	0.0
9	1.8419394886039204E-4	0.0	0.0	5.65659616950264	0.0
10	1.8419394886039204E-4	0.0	0.0	6.582170762526109	0.0
11	1.8419394886039204E-4	0.0	0.0	7.8619503192081135	0.0
12	3.6838789772078407E-4	0.0	0.0	8.966008848677303	0.0
13	3.6838789772078407E-4	0.0	0.0	9.430914375600933	0.0
14	3.6838789772078407E-4	0.0	0.0	9.624502215853205	0.0
15	3.6838789772078407E-4	0.0	0.0	9.949420341642936	0.0
16	3.6838789772078407E-4	0.0	0.0	10.622096642881088	0.0
17	3.6838789772078407E-4	0.0	0.0	11.432918405764534	0.0
18	3.6838789772078407E-4	0.0	0.0	12.276158303647408	0.0
19	5.525818465811762E-4	0.0	0.0	12.820451422529867	0.0
20	5.525818465811762E-4	0.0	0.0	13.319248636043808	0.0
21	5.525818465811762E-4	0.0	0.0	13.947350001657746	0.0
22	5.525818465811762E-4	0.0	0.0	14.610264023606296	0.0
23	5.525818465811762E-4	0.0	0.0	15.253285099077925	0.0
24	5.525818465811762E-4	0.0	0.0	15.759634264495142	0.0
25	5.525818465811762E-4	0.0	0.0	16.216251063720055	0.0
26	5.525818465811762E-4	0.0	0.0	16.65942170467816	0.0
27	5.525818465811762E-4	0.0	0.0	17.072384538023158	0.0
28	5.525818465811762E-4	0.0	0.0	17.539868780230833	0.0
29	5.525818465811762E-4	0.0	0.0	18.029456296301753	0.0
30	5.525818465811762E-4	0.0	0.0	18.5593822871731	0.0
31	5.525818465811762E-4	0.0	0.0	19.063705319152856	0.0
32	5.525818465811762E-4	0.0	0.0	19.55900284763845	0.0
33	5.525818465811762E-4	0.0	0.0	20.041406799703815	0.0
34	5.525818465811762E-4	0.0	0.0	20.505207162934283	0.0
35	5.525818465811762E-4	0.0	0.0	21.00639889778341	0.0
36	5.525818465811762E-4	0.0	0.0	21.49948609888268	0.0
37	5.525818465811762E-4	0.0	0.0	21.963286462113146	0.0
38	5.525818465811762E-4	0.0	0.0	22.458952378496463	0.0
39	5.525818465811762E-4	0.0	0.0	22.982431581157694	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	60	0.0	45.000004	14
GCATACG	30	2.1633768E-6	45.000004	1
GTTTACG	25	3.8880775E-5	45.0	1
CGCATCG	25	3.8880775E-5	45.0	21
ATTGTCG	20	7.029994E-4	45.0	11
GTCAACG	25	3.8880775E-5	45.0	1
GACGATG	20	7.029994E-4	45.0	1
CGTTTTT	2230	0.0	42.275787	1
GTTAGCG	65	0.0	41.53846	1
TCAACGG	65	0.0	41.53846	2
CGGTCTA	45	1.9250365E-8	40.000004	31
CCCGATT	90	0.0	40.000004	42
CCGCGAG	40	3.4537334E-7	39.375	16
ATCGAGG	40	3.4537334E-7	39.375	2
CTCGATC	70	0.0	38.57143	16
AAGGGCG	135	0.0	38.333332	5
CAAGGGC	235	0.0	38.29787	4
TCGATCA	65	9.094947E-12	38.07692	17
CTACGAA	65	9.094947E-12	38.07692	11
AGGGCGC	65	9.094947E-12	38.07692	6
>>END_MODULE