##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551694_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	153307
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.779599105063696	31.0	31.0	34.0	30.0	34.0
2	31.879835884858487	33.0	31.0	34.0	30.0	34.0
3	31.276823628405747	31.0	31.0	34.0	28.0	34.0
4	35.35105376792971	37.0	35.0	37.0	33.0	37.0
5	35.547913663433505	37.0	35.0	37.0	33.0	37.0
6	35.5512272759887	37.0	35.0	37.0	33.0	37.0
7	30.051935006229332	37.0	35.0	37.0	0.0	37.0
8	31.97782227817386	37.0	33.0	37.0	17.0	37.0
9	36.01879888067733	39.0	34.0	39.0	32.0	39.0
10	36.527829779462124	38.0	35.0	39.0	32.0	39.0
11	36.88361262042829	39.0	37.0	39.0	33.0	39.0
12	37.06190845819173	39.0	37.0	39.0	34.0	39.0
13	36.941959597409124	39.0	37.0	39.0	33.0	39.0
14	37.95111116909208	40.0	37.0	41.0	33.0	41.0
15	38.18181818181818	40.0	37.0	41.0	33.0	41.0
16	38.1262629886437	40.0	37.0	41.0	33.0	41.0
17	38.00974515188478	40.0	37.0	41.0	33.0	41.0
18	37.761576444650274	39.0	36.0	40.0	33.0	41.0
19	37.408239675944344	39.0	36.0	40.0	33.0	41.0
20	37.253530497628944	39.0	35.0	40.0	33.0	41.0
21	37.11425440456078	39.0	35.0	40.0	33.0	41.0
22	37.1697704605791	39.0	35.0	40.0	33.0	41.0
23	37.120927289686705	38.0	35.0	40.0	33.0	41.0
24	37.008512331465624	38.0	35.0	40.0	33.0	41.0
25	36.659271918438165	38.0	35.0	40.0	32.0	41.0
26	36.74915692042764	38.0	35.0	40.0	33.0	41.0
27	36.79677379376023	38.0	35.0	40.0	33.0	41.0
28	36.71309855388208	38.0	35.0	40.0	33.0	41.0
29	36.70088776115898	38.0	35.0	40.0	33.0	41.0
30	36.40733952135258	38.0	35.0	40.0	32.0	41.0
31	36.114932781934286	38.0	35.0	40.0	31.0	41.0
32	35.71200923636886	38.0	35.0	40.0	29.0	41.0
33	35.21556093329072	38.0	35.0	40.0	24.0	41.0
34	34.7131115996008	38.0	35.0	40.0	21.0	41.0
35	34.393217530836814	38.0	34.0	40.0	18.0	41.0
36	34.143072397216045	38.0	34.0	40.0	16.0	41.0
37	33.98124677933819	38.0	34.0	40.0	15.0	41.0
38	33.84582569615217	38.0	34.0	40.0	15.0	41.0
39	33.758001917720655	38.0	33.0	40.0	15.0	41.0
40	33.69319078711344	38.0	33.0	40.0	15.0	41.0
41	33.49159529571383	38.0	33.0	40.0	12.0	41.0
42	33.41538220694424	38.0	33.0	40.0	12.0	41.0
43	33.341204250295156	38.0	33.0	40.0	10.0	41.0
44	33.20811182790088	38.0	33.0	40.0	10.0	41.0
45	33.125597656988916	37.0	33.0	40.0	10.0	41.0
46	32.99448818384027	37.0	33.0	40.0	10.0	41.0
47	32.92128213323593	37.0	33.0	40.0	10.0	41.0
48	32.872875993920694	37.0	32.0	40.0	10.0	41.0
49	32.79166639488086	37.0	32.0	40.0	10.0	41.0
50	32.59054707221458	36.0	32.0	40.0	10.0	41.0
51	31.028067863828788	35.0	28.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	3.0
10	9.0
11	5.0
12	5.0
13	4.0
14	2.0
15	10.0
16	11.0
17	53.0
18	83.0
19	144.0
20	243.0
21	378.0
22	669.0
23	1059.0
24	1924.0
25	3174.0
26	4477.0
27	5068.0
28	4381.0
29	3799.0
30	3628.0
31	3901.0
32	4705.0
33	6553.0
34	10863.0
35	14349.0
36	13433.0
37	20104.0
38	26876.0
39	23388.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.106590044812044	17.04618836713262	19.81188073603945	26.035340852015892
2	45.086003900669894	18.582974032496885	20.847058516571323	15.483963550261892
3	27.970673224314613	19.331798287097133	37.71517282315876	14.982355665429498
4	25.274775450566512	21.32192267802514	37.40794614727312	15.995355724135232
5	21.14189175967177	23.809741238169163	37.69495195914081	17.35341504301826
6	21.867886006509814	27.131833510537678	38.261136151643434	12.73914433130907
7	54.312588466280076	20.8685839524614	21.094274886339175	3.724552694919345
8	63.51177702257562	6.217589542551873	24.951241626279298	5.319391808593215
9	59.52370080948685	6.747898008571037	27.602131670439057	6.126269511503062
10	33.812546067694235	19.757741003346226	33.69187316952259	12.737839759436948
11	27.017683471726667	20.811835076023925	37.08376003704984	15.086721415199566
12	24.463331746104224	19.794921301701816	40.903546478634375	14.83820047355959
13	20.437422948723803	24.285257685559042	41.309268330865514	13.968051034851637
14	16.79505828174839	25.876835369552598	40.85527731936572	16.4728290293333
15	15.495704697111027	21.2116863548305	48.03759776135467	15.255011186703804
16	17.144031257542057	21.061008303599966	44.93793499318362	16.857025445674363
17	17.79892633734924	20.13671913219879	40.820706164754384	21.243648365697588
18	18.816492397607416	19.99778222781739	43.371144174760445	17.81458119981475
19	19.98995479658463	21.26843523126798	40.49521548265898	18.246394489488413
20	22.055092070159876	21.189508633004365	40.55587807471283	16.19952122212293
21	19.465516903990032	23.121579575622768	41.40058836191433	16.012315158472866
22	18.31227536903077	21.65393621948117	39.83640668723542	20.197381724252644
23	17.107503245122533	23.998904159627415	40.144285649057124	18.749306946192934
24	18.494263145192328	20.817053363512432	42.30530895523362	18.383374536061627
25	16.44869444969897	22.179678683947895	41.60997214739053	19.761654718962603
26	15.70117476697085	23.740598928946493	40.97007964411279	19.588146659969865
27	17.104894101378278	23.443156542101796	41.57931470839557	17.872634648124354
28	14.063284781516824	22.123582093446483	44.64571089382742	19.167422231209272
29	15.739007351262499	20.73682219337669	43.26090785156581	20.263262603795
30	17.87720064967679	20.5887532858904	42.489253589203365	19.044792475229443
31	17.955474962004345	21.656545363225423	39.66355091417874	20.724428760591493
32	18.950211014500315	21.959206037558623	38.61076141337317	20.479821534567893
33	18.79366238984521	22.115102376277665	38.91929266113093	20.17194257274619
34	15.651601035830067	22.29839472431135	39.898373851161395	22.15163038869719
35	16.149947490982147	23.075919560098367	38.473781366799955	22.30035158211954
36	20.667679884154015	23.52664914191785	37.128767766638184	18.676903207289946
37	17.873939219996476	24.496598328843433	37.13268148225456	20.49678096890553
38	17.439516786578565	26.327564951372086	35.78962473990098	20.44329352214837
39	19.309620565270993	23.90497498483435	35.39499174858291	21.39041270131175
40	20.23195287886398	22.490819075449913	36.23252689048771	21.044701155198393
41	17.793708049860737	22.546915665951325	35.481093492143216	24.17828279204472
42	20.796832499494478	22.354491314812762	34.942305308955234	21.906370876737526
43	21.23973465008121	22.235122988513247	34.36111853992316	22.164023821482385
44	18.500786004552953	24.02042959551749	35.20974254274103	22.26904185718852
45	18.87519813185308	23.693634341549963	33.8588583691547	23.572309157442255
46	19.681423548826864	23.72885778209736	34.984703894799324	21.60501477427645
47	17.126419537268355	23.89192926611309	37.30358039750305	21.678070799115503
48	18.5686237419035	21.612842205509207	37.378593280150284	22.439940772437005
49	19.70947184407757	20.292615470917834	38.08828037858676	21.90963230641784
50	17.725870312510192	22.41906762248299	36.96047799513394	22.89458406987287
51	17.297970738452907	21.892672872080205	34.29197623070049	26.517380158766397
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	100.0
1	183.5
2	267.0
3	4600.5
4	8934.0
5	5787.0
6	2640.0
7	2569.0
8	2498.0
9	2428.0
10	2358.0
11	2258.0
12	2158.0
13	2098.0
14	2038.0
15	1910.0
16	1782.0
17	1593.0
18	1404.0
19	1326.0
20	1248.0
21	1133.0
22	1018.0
23	1041.0
24	1064.0
25	1163.5
26	1377.5
27	1492.0
28	1695.0
29	1898.0
30	2062.5
31	2227.0
32	2310.0
33	2393.0
34	3013.5
35	3634.0
36	3884.0
37	4134.0
38	4484.5
39	4835.0
40	5438.5
41	6042.0
42	7418.0
43	8794.0
44	9689.0
45	10584.0
46	11568.0
47	12552.0
48	13581.5
49	14611.0
50	14558.5
51	14506.0
52	12347.5
53	10189.0
54	8859.5
55	7530.0
56	6293.0
57	5056.0
58	4601.5
59	4147.0
60	3508.5
61	2870.0
62	2693.0
63	2516.0
64	2100.0
65	1684.0
66	1371.5
67	1059.0
68	881.0
69	703.0
70	562.5
71	422.0
72	358.5
73	295.0
74	237.5
75	136.5
76	93.0
77	67.0
78	41.0
79	29.5
80	18.0
81	14.5
82	11.0
83	9.5
84	8.0
85	6.0
86	4.0
87	5.0
88	6.0
89	3.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	153307.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.490845166887354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.69544996511898	39.73399779527354
2	8.707335348680981	8.79281441812833
3	3.35116140867633	5.076089154441741
4	1.8848668061907345	3.806740722863274
5	1.2893057385732372	3.2549068209540337
6	0.9004469937730926	2.727859784615184
7	0.7660904839418132	2.707638920597233
8	0.6588636539803117	2.661326619136765
9	0.6433609797690101	2.923545565434064
>10	3.0617781567320366	22.266432713444264
>50	0.025837790352169085	0.8740631543243296
>100	0.011627005658476087	1.0475712133170696
>500	0.0025837790352169084	1.0423529258285662
>1k	0.0012918895176084542	3.084660191641608
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4729	3.084660191641608	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	965	0.6294559283007299	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	633	0.4128969975278363	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	344	0.2243863620056488	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT	262	0.17089891524848833	TruSeq Adapter, Index 13 (95% over 22bp)
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	215	0.14024147625353048	No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	203	0.1324140450207753	TruSeq Adapter, Index 16 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20351321205163495	0.0
2	0.0	0.0	0.0	1.19890155048367	0.0
3	0.0	0.0	0.0	1.6398468432622124	0.0
4	0.0	0.0	0.0	2.1747213108338173	0.0
5	0.0	0.0	0.0	3.384059436294494	0.0
6	0.0	0.0	0.0	4.027213369252546	0.0
7	0.0	0.0	0.0	4.738205039561142	0.0
8	0.0	0.0	0.0	6.038210910134566	0.0
9	0.0	0.0	0.0	6.468067342000039	0.0
10	0.0	0.0	0.0	7.536511705271122	0.0
11	0.0	0.0	0.0	9.106563953374602	0.0
12	0.0	0.0	0.0	10.283287782032131	0.0
13	0.0	0.0	0.0	10.825989680836491	0.0
14	0.0	0.0	0.0	11.047114613161826	0.0
15	0.0	0.0	0.0	11.45283646539297	0.0
16	0.0	0.0	0.0	12.290371607297775	0.0
17	0.0	0.0	0.0	13.275975656688866	0.0
18	0.0	0.0	0.0	14.418128330735062	0.0
19	0.0	0.0	0.0	14.947784510818162	0.0
20	0.0	0.0	0.0	15.483963550261892	0.0
21	0.0	0.0	0.0	16.15320892066246	0.0
22	0.0	0.0	0.0	16.793101423940197	0.0
23	0.0	0.0	0.0	17.463651366212893	0.0
24	0.0	0.0	0.0	17.992655260359932	0.0
25	0.0	0.0	0.0	18.47730371085469	0.0
26	0.0	0.0	0.0	18.95542930198882	0.0
27	0.0	0.0	0.0	19.448557469652396	0.0
28	0.0	0.0	0.0	19.879066187453933	0.0
29	0.0	0.0	0.0	20.32392519584885	0.0
30	0.0	0.0	0.0	20.85488594780408	0.0
31	0.0	0.0	0.0	21.350623259211908	0.0
32	0.0	0.0	0.0	21.79548226760683	0.0
33	0.0	0.0	0.0	22.25469156659513	0.0
34	0.0	0.0	0.0	22.668893135995095	0.0
35	0.0	0.0	0.0	23.133973008407967	0.0
36	0.0	0.0	0.0	23.554045151232494	0.0
37	0.0	0.0	0.0	23.972160436248835	0.0
38	0.0	0.0	0.0	24.406582869666746	0.0
39	0.0	0.0	0.0	24.93232533413347	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAC	35	1.202734E-7	45.0	26
GAGCTAC	20	7.0141506E-4	45.0	21
CAACGAG	50	2.1827873E-11	45.0	14
CGTGCAG	25	3.8749527E-5	45.0	2
GACACGA	35	1.202734E-7	45.0	25
GGGTACA	20	7.0141506E-4	45.0	7
TGAACGG	25	3.8749527E-5	45.0	2
CGACCAA	35	1.202734E-7	45.0	29
TTGTAGG	40	6.7484507E-9	45.0	2
CACGACC	35	1.202734E-7	45.0	27
AGGCGAT	20	7.0141506E-4	45.0	7
ACGGGTG	25	3.8749527E-5	45.0	5
ATCGTGG	20	7.0141506E-4	45.0	2
CAGGGTA	20	7.0141506E-4	45.0	5
CAGGGCG	20	7.0141506E-4	45.0	5
ACCTAGG	20	7.0141506E-4	45.0	3
TCGTGGG	25	3.8749527E-5	45.0	3
GTCACTT	35	1.202734E-7	45.0	4
ATGACGG	20	7.0141506E-4	45.0	2
TGCTCAC	20	7.0141506E-4	45.0	45
>>END_MODULE