##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551693_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	152607
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.297882796988343	31.0	31.0	34.0	28.0	34.0
2	31.45702359655848	31.0	31.0	34.0	28.0	34.0
3	31.358142155995466	31.0	31.0	34.0	28.0	34.0
4	35.173891105912574	37.0	35.0	37.0	32.0	37.0
5	32.877010884166516	37.0	35.0	37.0	28.0	37.0
6	34.028622540250446	37.0	35.0	37.0	28.0	37.0
7	35.263310333077776	37.0	35.0	37.0	32.0	37.0
8	35.06875831383881	37.0	35.0	37.0	33.0	37.0
9	37.018406757226074	39.0	37.0	39.0	32.0	39.0
10	36.477114418080426	38.0	35.0	39.0	32.0	39.0
11	36.66813448924361	39.0	35.0	39.0	32.0	39.0
12	36.695918273735806	39.0	35.0	39.0	33.0	39.0
13	36.599972478326684	39.0	35.0	39.0	32.0	39.0
14	37.500737187678155	40.0	36.0	41.0	32.0	41.0
15	37.81091955152778	40.0	36.0	41.0	33.0	41.0
16	37.93051432765208	40.0	36.0	41.0	33.0	41.0
17	37.92128801431127	40.0	36.0	41.0	33.0	41.0
18	37.679169369688154	39.0	36.0	40.0	33.0	41.0
19	37.344715511084026	38.0	36.0	40.0	33.0	41.0
20	37.17514268677059	39.0	35.0	40.0	33.0	41.0
21	36.991238933993856	38.0	35.0	40.0	33.0	41.0
22	37.13783771386634	38.0	35.0	40.0	33.0	41.0
23	37.204774355042694	39.0	35.0	40.0	33.0	41.0
24	37.05289403500495	38.0	35.0	40.0	33.0	41.0
25	36.65864606472835	38.0	35.0	40.0	32.0	41.0
26	36.81841593111719	38.0	35.0	40.0	33.0	41.0
27	36.78731644026814	38.0	35.0	40.0	33.0	41.0
28	36.73611957511778	38.0	35.0	40.0	33.0	41.0
29	36.792473477625535	38.0	35.0	40.0	33.0	41.0
30	36.32585005930265	38.0	35.0	40.0	31.0	41.0
31	36.026512545296086	38.0	35.0	40.0	31.0	41.0
32	35.65101207677236	38.0	35.0	40.0	29.0	41.0
33	35.26314651359374	38.0	35.0	40.0	24.0	41.0
34	34.8670572123166	38.0	35.0	40.0	21.0	41.0
35	34.67040175090265	38.0	35.0	41.0	18.0	41.0
36	34.4396521784715	38.0	35.0	40.0	18.0	41.0
37	34.4317822904585	38.0	34.0	40.0	18.0	41.0
38	34.23614906262491	38.0	34.0	40.0	18.0	41.0
39	34.17998519071865	38.0	34.0	40.0	17.0	41.0
40	33.992962314965894	38.0	34.0	40.0	15.0	41.0
41	33.81981167312115	38.0	34.0	40.0	15.0	41.0
42	33.93051432765208	38.0	34.0	40.0	15.0	41.0
43	33.88708250604494	38.0	34.0	40.0	15.0	41.0
44	33.87448151133303	38.0	34.0	40.0	15.0	41.0
45	33.82822544182115	38.0	34.0	40.0	15.0	41.0
46	33.76238311479814	38.0	34.0	40.0	15.0	41.0
47	33.736158891793956	38.0	34.0	40.0	15.0	41.0
48	33.71002640770082	37.0	34.0	40.0	15.0	41.0
49	33.716579187062194	37.0	34.0	40.0	14.0	41.0
50	33.64192992457751	37.0	34.0	40.0	13.0	41.0
51	32.84537406540984	36.0	32.0	40.0	11.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	8.0
10	12.0
11	7.0
12	5.0
13	9.0
14	7.0
15	15.0
16	26.0
17	45.0
18	91.0
19	167.0
20	256.0
21	449.0
22	637.0
23	1059.0
24	1699.0
25	2863.0
26	4045.0
27	4511.0
28	4015.0
29	3493.0
30	3299.0
31	3743.0
32	4497.0
33	6360.0
34	11115.0
35	13046.0
36	12671.0
37	17346.0
38	27788.0
39	29311.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.50941962033196	17.212840826436533	19.73172921294567	25.54601034028583
2	44.61918522741421	18.37333805133447	20.58883275341236	16.418643967838957
3	27.84079367263625	19.299245775095507	37.38491681246601	15.475043739802238
4	25.855301526142316	21.56781799000046	35.88367506077702	16.693205423080197
5	20.196976547602667	29.15986815807925	34.31428440372984	16.32887089058824
6	22.24996232151867	26.980413742488878	37.152948423073646	13.616675512918805
7	65.81480534968908	5.529890503056872	24.247249470863068	4.408054676390991
8	65.66998892580288	4.34252688277733	24.181721677249403	5.805762514170385
9	60.20890260604035	6.824064426926681	26.561691141297583	6.405341825735386
10	34.26120689090278	19.818225900515703	32.81304265204086	13.107524556540657
11	27.601617225946384	21.072427870281178	35.970827026283196	15.355127877489236
12	24.750502925815983	20.0239831724626	39.76488627651418	15.460627625207232
13	20.848322816122458	24.619447338588664	40.29631668272098	14.235913162567904
14	17.075232459847843	26.053195462855573	39.886112694699456	16.985459382597128
15	15.599546547668194	21.36075016218129	47.05550859397013	15.984194696180385
16	17.253468058477004	21.0960178759821	44.32889710170569	17.32161696383521
17	18.143335495750524	20.284128513108833	39.82189545695807	21.750640534182576
18	19.10331767219066	20.21532432981449	42.55637028445615	18.124987713538697
19	20.23629322377086	21.143197887383934	39.776681279364645	18.843827609480563
20	22.371188739703946	21.21527846035896	39.79502906157647	16.618503738360626
21	19.790704227197967	23.073646687242395	40.67637788568021	16.45927119987943
22	18.54436559266613	21.893491124260358	39.25180365251922	20.3103396305543
23	17.360933640003406	24.097190823487782	39.31077866677151	19.2310968697373
24	18.535191701560215	21.348299881394695	41.71433813652061	18.402170280524484
25	16.977596047363487	22.50224432693127	40.57677563938745	19.943383986317798
26	15.753536862660297	24.188274456610774	40.184919433577754	19.87326924715118
27	17.636150373180783	23.491058732561417	40.90506988539189	17.96772100886591
28	14.629735202186007	22.45768542727398	43.64478693637906	19.26779243416095
29	15.795474650573041	21.08553342900391	42.30212244523515	20.8168694751879
30	18.254732744893747	20.91581644354453	41.53479198201918	19.29465882954255
31	18.512256973795434	21.905286127110816	38.673848512846725	20.908608386247028
32	19.07055377538383	22.554011283886062	37.551357408244705	20.824077532485404
33	19.07645127680906	22.331872063535748	37.652925488345886	20.93875117130931
34	16.4533736984542	22.874442194656865	38.28133702910089	22.39084707778804
35	16.658475692464958	23.145071982281284	37.29121206759847	22.905240257655286
36	21.001657853178425	23.680434056104897	36.309605719265825	19.00830237145085
37	18.129574659091656	24.401895063791308	36.68770108841665	20.780829188700388
38	17.726578728367638	26.3061327462043	35.20218600719495	20.765102518233107
39	19.815604788771157	23.710576841167182	34.86013092453164	21.613687445530022
40	20.601938312135093	22.57956712339539	35.475436906563914	21.3430576579056
41	17.8995721035077	22.682445759368836	35.17007738832426	24.247904748799204
42	20.984620626838872	22.470135708060575	34.02334100008519	22.521902665015368
43	21.462318242282464	22.014717542445627	33.87131651890149	22.651647696370418
44	18.858899002011704	23.778070468589252	34.55280557248357	22.810224956915476
45	19.003060147961758	23.76299907605811	33.29008498954832	23.943855786431815
46	19.679306978054743	23.483195397327776	34.65240781877633	22.185089805841148
47	17.341930579855447	23.994967465450472	36.873144744343314	21.78995721035077
48	18.698355907658236	21.488529359727927	36.8639708532374	22.94914387937644
49	19.809052009409793	20.482022449822093	37.535630737777424	22.17329480299069
50	17.997208515992057	22.185745083777284	36.434763805067924	23.38228259516274
51	17.32816974319658	21.945913359151284	34.08231601433748	26.643600883314654
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	68.0
1	157.0
2	246.0
3	3996.5
4	7747.0
5	5009.0
6	2271.0
7	2174.0
8	2077.0
9	2141.0
10	2205.0
11	2219.0
12	2233.0
13	2093.5
14	1954.0
15	1825.5
16	1697.0
17	1528.5
18	1360.0
19	1298.5
20	1237.0
21	1133.0
22	1029.0
23	1070.0
24	1111.0
25	1127.0
26	1278.5
27	1414.0
28	1590.5
29	1767.0
30	1977.0
31	2187.0
32	2258.0
33	2329.0
34	2908.0
35	3487.0
36	3805.0
37	4123.0
38	4493.5
39	4864.0
40	5210.5
41	5557.0
42	7071.5
43	8586.0
44	9297.5
45	10009.0
46	11315.5
47	12622.0
48	13693.5
49	14765.0
50	15023.5
51	15282.0
52	12922.0
53	10562.0
54	9405.0
55	8248.0
56	6812.0
57	5376.0
58	4874.0
59	4372.0
60	3758.5
61	3145.0
62	2927.0
63	2709.0
64	2285.5
65	1862.0
66	1506.5
67	1151.0
68	952.5
69	754.0
70	578.5
71	403.0
72	358.0
73	313.0
74	241.5
75	128.0
76	86.0
77	60.5
78	35.0
79	27.5
80	20.0
81	15.0
82	10.0
83	9.0
84	8.0
85	8.0
86	8.0
87	5.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	152607.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.40870340154776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2326086055094	37.56315241109517
2	8.233700534907186	7.806981331131599
3	2.837634245117417	4.035856808665395
4	1.5107326984478016	2.8648751367892693
5	1.044934968002322	2.4769505985963947
6	0.9219201371131598	2.6224223004187226
7	0.7560574437794578	2.509059217467089
8	0.7477643091127728	2.8360429075992584
9	0.6040166415568978	2.5772081228252963
>10	4.066400364897926	28.76866723020569
>50	0.03040816044451202	0.9357368928030824
>100	0.01105751288891346	1.2902422562529898
>500	0.0013821891111141826	0.5242223489092899
>1k	0.0013821891111141826	3.188582437240756
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4866	3.188582437240756	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	800	0.5242223489092899	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	485	0.317809799026257	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT	341	0.22344977622258483	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	322	0.2109994954359892	No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	229	0.1500586473752842	TruSeq Adapter, Index 16 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	172	0.11270780501549732	TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTG	171	0.11205252707936073	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0013105558722732247	0.0	0.0	0.23393422320077062	0.0
2	0.0013105558722732247	0.0	0.0	1.3924656142903011	0.0
3	0.0013105558722732247	0.0	0.0	1.8564023930750229	0.0
4	0.0013105558722732247	0.0	0.0	2.4520500370232035	0.0
5	0.0013105558722732247	0.0	0.0	3.7586742416796084	0.0
6	0.0013105558722732247	0.0	0.0	4.5279705387039915	0.0
7	0.0013105558722732247	0.0	0.0	5.298577391600648	0.0
8	0.0013105558722732247	0.0	0.0	6.559987418663626	0.0
9	0.0013105558722732247	0.0	0.0	7.033753366490397	0.0
10	0.0013105558722732247	0.0	0.0	8.078266396692158	0.0
11	0.0013105558722732247	0.0	0.0	9.663383724206621	0.0
12	0.0013105558722732247	0.0	0.0	10.789151218489323	0.0
13	0.0013105558722732247	0.0	0.0	11.311407733590203	0.0
14	0.0013105558722732247	0.0	0.0	11.526994174579148	0.0
15	0.0013105558722732247	0.0	0.0	11.972583171152044	0.0
16	0.0013105558722732247	0.0	0.0	12.814615319087592	0.0
17	0.0013105558722732247	0.0	0.0	13.827019730418657	0.0
18	0.0013105558722732247	0.0	0.0	14.92526555138362	0.0
19	0.0013105558722732247	0.0	0.0	15.471767350121555	0.0
20	0.0013105558722732247	0.0	0.0	16.037927486943587	0.0
21	0.0013105558722732247	0.0	0.0	16.778391554777958	0.0
22	0.0013105558722732247	0.0	0.0	17.448740883445712	0.0
23	0.0013105558722732247	0.0	0.0	18.140059106069838	0.0
24	0.0013105558722732247	0.0	0.0	18.62562005674707	0.0
25	0.0013105558722732247	0.0	0.0	19.134771013125217	0.0
26	0.0013105558722732247	0.0	0.0	19.616400296185628	0.0
27	0.0013105558722732247	0.0	0.0	20.084924020523307	0.0
28	0.0013105558722732247	0.0	0.0	20.514131068692787	0.0
29	0.0013105558722732247	0.0	0.0	21.021316191262525	0.0
30	0.0013105558722732247	0.0	0.0	21.553401875405452	0.0
31	0.0013105558722732247	0.0	0.0	22.059931720039053	0.0
32	0.0013105558722732247	0.0	0.0	22.504865438675814	0.0
33	0.0013105558722732247	0.0	0.0	22.991736945225316	0.0
34	0.0013105558722732247	0.0	0.0	23.41504649196957	0.0
35	0.0013105558722732247	0.0	0.0	23.842942984266777	0.0
36	0.0013105558722732247	0.0	0.0	24.27149475450012	0.0
37	0.0013105558722732247	0.0	0.0	24.721015418689838	0.0
38	0.0013105558722732247	0.0	0.0	25.157430524156823	0.0
39	0.0013105558722732247	0.0	0.0	25.665926202598833	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	20	7.01405E-4	45.0	13
ATGGGTC	25	3.8748687E-5	45.0	5
CAACGAC	20	7.01405E-4	45.0	12
GGCCGAT	20	7.01405E-4	45.0	8
CGATTGG	20	7.01405E-4	45.0	10
TGACGGG	40	6.7484507E-9	45.0	3
ACGGGTG	25	3.8748687E-5	45.0	5
CGTTATT	205	0.0	45.0	1
CTTGCGG	20	7.01405E-4	45.0	2
TGCGACG	20	7.01405E-4	45.0	1
GCGGGTG	25	3.8748687E-5	45.0	5
CAGGCCG	20	7.01405E-4	45.0	26
GACGGGT	25	3.8748687E-5	45.0	4
CGTAAGG	20	7.01405E-4	45.0	2
CTCGACG	20	7.01405E-4	45.0	1
CACAACG	20	7.01405E-4	45.0	12
ACATATC	20	7.01405E-4	45.0	36
CGGGTAT	30	2.1531032E-6	44.999996	6
ATAGCGG	30	2.1531032E-6	44.999996	2
GTAGGGC	45	3.8016879E-10	44.999996	4
>>END_MODULE