FastQCFastQC Report
Sat 18 Jun 2016
SRR3551691_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551691_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183391
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24011.3092245530042368No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGC22021.2007132302021364No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTC21441.169086814511072No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCG20711.1292811533826632No Hit
GCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC10080.5496452933895338No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATTCCGAT7830.42695661182937No Hit
GAACTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCT4540.24755849523695272No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCT4510.2459226461494839No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATTCCGATCGTA4400.23992453282876477No Hit
CCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC3890.21211509834179432No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTC3550.19357547535048067No Hit
CTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGCT2630.14340943666810257Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAC453.8016879E-1045.00000434
CGAATGC900.045.00000445
TAATAGG207.0177665E-445.02
ATAGGGC551.8189894E-1245.04
CGCATGG207.0177665E-445.02
TAAACGG207.0177665E-445.02
CGATTGT207.0177665E-445.010
TACGGGA207.0177665E-445.04
ACGATGG253.8779475E-545.02
CTACGAA207.0177665E-445.011
AGTTAGG207.0177665E-445.02
GCTACGA207.0177665E-445.010
CGATCAC253.8779475E-545.034
CGATGAA5300.044.15094419
CGTTTTT12800.043.417971
CCGATGA5400.043.33333218
ATGAATG5900.043.0932221
CGATCGA1000.042.7541
TCGAATG1000.042.7544
GATGAAT5950.042.7310920