##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551691_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	183391
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21248589080162	31.0	31.0	33.0	28.0	34.0
2	31.267570382406987	31.0	31.0	34.0	28.0	34.0
3	31.253218533079593	31.0	31.0	34.0	28.0	34.0
4	35.148682323560045	37.0	35.0	37.0	33.0	37.0
5	32.90139646983767	35.0	35.0	37.0	28.0	37.0
6	33.983025339302365	36.0	35.0	37.0	28.0	37.0
7	35.16833977676113	37.0	35.0	37.0	32.0	37.0
8	34.53539704783768	36.0	35.0	37.0	31.0	37.0
9	36.30027645849578	39.0	35.0	39.0	32.0	39.0
10	36.01446090593323	37.0	35.0	39.0	31.0	39.0
11	36.37535102595002	38.0	35.0	39.0	32.0	39.0
12	36.52707602881276	39.0	35.0	39.0	32.0	39.0
13	36.53424104781587	39.0	35.0	39.0	32.0	39.0
14	37.50658429257706	39.0	36.0	41.0	32.0	41.0
15	37.640538521519595	39.0	36.0	41.0	32.0	41.0
16	37.80802220392495	39.0	37.0	41.0	33.0	41.0
17	37.62278955892056	39.0	36.0	41.0	32.0	41.0
18	37.55739376523385	39.0	36.0	40.0	33.0	41.0
19	37.374156856116166	39.0	36.0	40.0	32.0	41.0
20	37.10711539824746	39.0	35.0	40.0	32.0	41.0
21	37.20424121140078	39.0	35.0	40.0	32.0	41.0
22	37.354881101035495	39.0	35.0	41.0	33.0	41.0
23	37.26494757103675	39.0	35.0	41.0	32.0	41.0
24	37.19545124897078	39.0	35.0	41.0	32.0	41.0
25	36.9468730744693	39.0	35.0	40.0	32.0	41.0
26	36.99033758472335	39.0	35.0	40.0	32.0	41.0
27	36.79773816599506	39.0	35.0	41.0	31.0	41.0
28	36.71825225883495	39.0	35.0	41.0	31.0	41.0
29	36.82033469472329	39.0	35.0	41.0	31.0	41.0
30	36.443173329116476	39.0	35.0	40.0	30.0	41.0
31	36.34230687438315	39.0	35.0	40.0	30.0	41.0
32	36.00703415107612	39.0	35.0	40.0	29.0	41.0
33	35.82313744949316	39.0	35.0	41.0	27.0	41.0
34	35.63385335158214	39.0	35.0	41.0	26.0	41.0
35	35.4478736688278	39.0	35.0	41.0	24.0	41.0
36	35.26352438233065	39.0	35.0	41.0	23.0	41.0
37	35.164402833290616	39.0	35.0	41.0	23.0	41.0
38	35.06897830318827	39.0	35.0	40.0	23.0	41.0
39	34.94386856497865	39.0	35.0	40.0	22.0	41.0
40	34.90923218696665	38.0	35.0	40.0	22.0	41.0
41	34.78301007137755	38.0	34.0	40.0	23.0	41.0
42	34.81724293994798	38.0	35.0	40.0	22.0	41.0
43	34.785867354450325	38.0	34.0	40.0	22.0	41.0
44	34.92801718732108	38.0	35.0	40.0	22.0	41.0
45	34.88856596016162	38.0	35.0	40.0	23.0	41.0
46	34.7642359766837	38.0	34.0	40.0	22.0	41.0
47	34.60445169065003	38.0	34.0	40.0	22.0	41.0
48	34.61699320032063	38.0	34.0	40.0	22.0	41.0
49	34.65199491796217	38.0	34.0	40.0	22.0	41.0
50	34.4904002922717	37.0	34.0	40.0	22.0	41.0
51	33.61885806828034	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	9.0
10	8.0
11	3.0
12	12.0
13	10.0
14	15.0
15	23.0
16	38.0
17	52.0
18	76.0
19	192.0
20	389.0
21	815.0
22	1379.0
23	1839.0
24	2176.0
25	2758.0
26	3258.0
27	3420.0
28	3303.0
29	3331.0
30	3663.0
31	4476.0
32	5588.0
33	7598.0
34	12314.0
35	14624.0
36	16496.0
37	23581.0
38	35416.0
39	36520.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.94014428188951	14.707919145432435	18.17319279572062	20.178743776957432
2	33.47874214110834	29.081034510962915	21.320021156981532	16.12020219094721
3	28.955074131227814	28.564106199322758	28.13387788931845	14.346941780130976
4	26.015998604075445	21.008664547333293	35.39813840373846	17.5771984448528
5	27.474630707068503	29.100119416983382	25.48870991488132	17.936539961066792
6	22.361511742670032	36.401459177386016	27.538428821479787	13.698600258464156
7	68.11293902099885	5.301786892486545	22.09541362444177	4.489860462072839
8	67.5060390095479	11.758483240726099	15.094524813104243	5.640952936621754
9	62.09246909608432	7.041785038524247	16.402658800050165	14.463087065341265
10	38.95338375383743	22.152123059474018	23.44717025372019	15.447322932968358
11	31.907781734109093	21.46724757485373	28.456685442578973	18.16828524845821
12	24.70950046621699	19.171060739076616	32.570845897563125	23.548592897143262
13	26.46640238615853	20.509185292626135	35.826730864655296	17.197681456560026
14	19.621464521159705	28.87491752594184	31.359227006777868	20.144390946120584
15	17.802945618923502	22.395319290477723	37.40696108315021	22.394774007448568
16	20.06914188809702	22.066513623896483	32.11498928518848	25.749355202818023
17	20.329241893004564	25.32730613825106	32.65154778587826	21.691904182866118
18	20.560987180395983	21.597570218822078	33.01470628329635	24.82673631748559
19	21.107906058639735	23.413362705912505	31.072408133441666	24.4063231020061
20	26.116330681440203	22.314617402162593	33.44766100844643	18.121390907950772
21	22.586168350682424	28.19822128675889	30.692345862119737	18.523264500438952
22	20.356506044462378	21.305298515194313	35.23400821196242	23.10418722838089
23	23.069289114514888	24.547006123528416	31.828170411852273	20.55553435010442
24	21.56976078433511	24.88017405434291	31.227268513722045	22.322796647599937
25	20.054419246309795	28.65298733307523	29.84279490269424	21.449798517920726
26	20.236543778047995	22.741028730962807	34.71707990032226	22.305347590666933
27	24.145132531040236	24.46848536732991	30.16124019172151	21.225141909908338
28	18.502543745331014	26.83283258175156	33.840264789438955	20.82435888347847
29	22.064877774809013	22.87789477128103	33.56598742577334	21.491240028136605
30	20.321607930596375	27.804526939708058	31.562072293624006	20.31179283607156
31	22.475475895763697	22.29444193008381	29.647038295227134	25.583043878925356
32	25.72754388165177	24.660424993592926	30.101259058514323	19.510772066240982
33	22.781924958149528	23.511513651160634	28.102796756656545	25.603764634033293
34	22.431307970402038	23.031664585503105	29.413657158748247	25.123370285346606
35	24.30326460949556	24.439040083755472	27.874323167440057	23.383372139308907
36	22.83154571380275	28.77949299583949	27.388475988461813	21.00048530189595
37	20.692400390422648	26.681243899646113	30.7414213347438	21.88493437518744
38	20.4235758570486	25.280957080772776	28.305096760473525	25.990370301705102
39	21.530500406235856	24.740581598878897	29.49108734888844	24.237830645996805
40	22.95641552747954	22.46457023518057	28.87437224291268	25.704641994427206
41	20.179289059986587	25.822423128724964	26.96969862206979	27.028589189218664
42	23.20124760757071	24.385602346898157	29.84224961966509	22.570900425866043
43	22.265541929538525	25.878041997698904	28.357443931272524	23.49897214149004
44	21.550130595285484	23.542594783822544	30.853749638749996	24.05352498214198
45	21.598115501851236	21.77206078815209	29.540708104541665	27.08911560545501
46	24.570453293782137	24.772208014569962	28.572285444760105	22.0850532468878
47	19.46551357482101	25.214432551215708	33.05669307654138	22.263360797421903
48	20.93886831960129	25.141909908337922	29.494904330092535	24.424317441968256
49	21.439438140366757	22.306983439754404	33.430211951513435	22.823366468365407
50	21.513596632332014	23.069834397544046	31.124210021211514	24.292358948912433
51	20.835264544061598	22.56435702951617	29.38530244123212	27.21507598519011
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	46.0
1	191.5
2	337.0
3	2324.0
4	4311.0
5	2903.0
6	1495.0
7	1442.0
8	1389.0
9	1335.0
10	1281.0
11	1269.5
12	1258.0
13	1166.0
14	1074.0
15	1032.5
16	991.0
17	893.5
18	796.0
19	835.0
20	874.0
21	851.0
22	828.0
23	877.0
24	926.0
25	1074.5
26	1226.0
27	1229.0
28	1435.0
29	1641.0
30	1979.0
31	2317.0
32	2625.5
33	2934.0
34	3298.5
35	3663.0
36	3928.0
37	4193.0
38	4512.0
39	4831.0
40	5627.0
41	6423.0
42	7130.0
43	7837.0
44	8884.5
45	9932.0
46	15362.5
47	20793.0
48	18482.5
49	16172.0
50	16062.5
51	15953.0
52	14572.5
53	13192.0
54	12190.5
55	11189.0
56	10112.0
57	9035.0
58	8607.5
59	8180.0
60	7629.5
61	7079.0
62	6403.0
63	5727.0
64	5139.0
65	4551.0
66	3814.0
67	3077.0
68	2585.0
69	2093.0
70	1812.0
71	1531.0
72	1313.5
73	1096.0
74	936.5
75	625.0
76	473.0
77	382.0
78	291.0
79	243.5
80	196.0
81	151.5
82	107.0
83	70.0
84	33.0
85	21.5
86	10.0
87	7.0
88	4.0
89	2.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	183391.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.573861312714364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.21189255780189	36.789700694145296
2	9.16140528411466	9.083324699685372
3	4.749543524649669	7.063596359690498
4	3.311921156257562	6.567388803158279
5	2.548562377631608	6.317103892775545
6	1.8654992630397957	5.548800104694342
7	1.2726312779109927	4.416247253136741
8	0.9151505818685792	3.6294038420642236
9	0.5785687572871065	2.5813698600258466
>10	1.3309281298809865	9.09095866209356
>50	0.024198693270563388	0.8097452982970811
>100	0.023098752667355962	2.3174528739142053
>500	0.0010999406032074267	0.42695661182937
>1k	0.005499703016037134	5.357951044489642
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2401	1.3092245530042368	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGC	2202	1.2007132302021364	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTC	2144	1.169086814511072	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCG	2071	1.1292811533826632	No Hit
GCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC	1008	0.5496452933895338	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATTCCGAT	783	0.42695661182937	No Hit
GAACTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCT	454	0.24755849523695272	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCT	451	0.2459226461494839	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATTCCGATCGTA	440	0.23992453282876477	No Hit
CCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC	389	0.21211509834179432	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTC	355	0.19357547535048067	No Hit
CTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGCT	263	0.14340943666810257	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15213396513460312	0.0
2	0.0	0.0	0.0	1.0676641710880033	0.0
3	0.0	0.0	0.0	1.277052854284016	0.0
4	0.0	0.0	0.0	1.7743509768745467	0.0
5	0.0	0.0	0.0	3.6326755402391613	0.0
6	0.0	0.0	0.0	4.282652910993451	0.0
7	0.0	0.0	0.0	5.037869906374904	0.0
8	0.0	0.0	0.0	5.982300112873587	0.0
9	0.0	0.0	0.0	6.2778435146762925	0.0
10	0.0	0.0	0.0	8.178700154315097	0.0
11	0.0	0.0	0.0	9.137853002601	0.0
12	0.0	0.0	0.0	11.329345496780103	0.0
13	0.0	0.0	0.0	11.743760598938879	0.0
14	0.0	0.0	0.0	11.958056829397298	0.0
15	0.0	0.0	0.0	12.47880212224155	0.0
16	0.0	0.0	0.0	13.081339869459242	5.452830291562835E-4
17	0.0	0.0	0.0	13.701326673609938	5.452830291562835E-4
18	0.0	0.0	0.0	14.321313477760631	5.452830291562835E-4
19	0.0	0.0	0.0	15.198128588643936	5.452830291562835E-4
20	0.0	0.0	0.0	15.635445578027275	5.452830291562835E-4
21	0.0	0.0	0.0	16.098936152810115	5.452830291562835E-4
22	0.0	0.0	0.0	16.572787105146926	5.452830291562835E-4
23	0.0	0.0	0.0	17.051545604746142	5.452830291562835E-4
24	0.0	0.0	0.0	17.434334291213855	5.452830291562835E-4
25	0.0	0.0	0.0	17.76968335414497	5.452830291562835E-4
26	0.0	0.0	0.0	18.099034303755364	5.452830291562835E-4
27	0.0	0.0	0.0	18.506906009564265	5.452830291562835E-4
28	0.0	0.0	0.0	18.83789280826213	5.452830291562835E-4
29	0.0	0.0	0.0	19.17814941845565	5.452830291562835E-4
30	0.0	0.0	0.0	19.575115463681424	5.452830291562835E-4
31	0.0	0.0	0.0	19.956813584090824	5.452830291562835E-4
32	0.0	0.0	0.0	20.327606043917097	5.452830291562835E-4
33	0.0	0.0	0.0	20.679858880752054	5.452830291562835E-4
34	0.0	0.0	0.0	21.037564547878578	5.452830291562835E-4
35	0.0	0.0	0.0	21.409447573763163	5.452830291562835E-4
36	0.0	0.0	0.0	21.75188531607331	5.452830291562835E-4
37	0.0	0.0	0.0	22.125949474074517	5.452830291562835E-4
38	0.0	0.0	0.0	22.48365514120104	5.452830291562835E-4
39	0.0	0.0	0.0	22.90461363970969	5.452830291562835E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAC	45	3.8016879E-10	45.000004	34
CGAATGC	90	0.0	45.000004	45
TAATAGG	20	7.0177665E-4	45.0	2
ATAGGGC	55	1.8189894E-12	45.0	4
CGCATGG	20	7.0177665E-4	45.0	2
TAAACGG	20	7.0177665E-4	45.0	2
CGATTGT	20	7.0177665E-4	45.0	10
TACGGGA	20	7.0177665E-4	45.0	4
ACGATGG	25	3.8779475E-5	45.0	2
CTACGAA	20	7.0177665E-4	45.0	11
AGTTAGG	20	7.0177665E-4	45.0	2
GCTACGA	20	7.0177665E-4	45.0	10
CGATCAC	25	3.8779475E-5	45.0	34
CGATGAA	530	0.0	44.150944	19
CGTTTTT	1280	0.0	43.41797	1
CCGATGA	540	0.0	43.333332	18
ATGAATG	590	0.0	43.09322	21
CGATCGA	100	0.0	42.75	41
TCGAATG	100	0.0	42.75	44
GATGAAT	595	0.0	42.73109	20
>>END_MODULE