FastQCFastQC Report
Sat 18 Jun 2016
SRR3551690_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551690_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229841
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC30861.3426673221922982TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC30621.3322253209827664No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG29041.2634821463533488No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27561.1990898055612358No Hit
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC13480.5864924012687032TruSeq Adapter, Index 13 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCT6620.2880252000295857No Hit
GAATCTATCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC6500.28280419942481977RNA PCR Primer, Index 13 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT5840.2540886960986073RNA PCR Primer, Index 13 (95% over 23bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG5340.23233452691208267No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC5210.2266784429235863No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5170.22493810938866432No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTC5090.22145744231882042No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTA4830.2101452743418276No Hit
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC3550.15445460122432464TruSeq Adapter, Index 13 (96% over 25bp)
GCTGTCACTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC2770.12051809729334627TruSeq Adapter, Index 13 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT2670.11616726345604134Illumina PCR Primer Index 4 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGAT207.021496E-445.00000413
CGGGTAT351.2062628E-745.0000046
GGTACCT351.2062628E-745.0000048
CTCGTTC207.021496E-445.00000415
TTTCGCA207.021496E-445.00000414
TAGCATA406.7739165E-945.00000430
AATGCGG207.021496E-445.0000042
CCCCGAT207.021496E-445.00000440
CCTCAAT207.021496E-445.00000438
TTCGCAA207.021496E-445.00000415
GGGTACC502.1827873E-1145.07
TGAAACG502.1827873E-1145.04
CGTTTTA2250.045.01
TGCGGGC650.044.9999964
CGTTAGG302.1578999E-644.9999962
CGTTTTT15200.043.0756571
TACGGCT4100.042.804887
ACGGCTG4700.042.127668
CGGTTTT603.6379788E-1241.2499961
GTCAAGC1150.041.08695616
Produced by FastQC (version 0.11.5)