##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551690_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	229841
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7507755361315	31.0	31.0	34.0	30.0	34.0
2	31.80392097145418	31.0	31.0	34.0	30.0	34.0
3	31.460666286693844	31.0	31.0	34.0	28.0	34.0
4	35.42582045849087	37.0	35.0	37.0	33.0	37.0
5	35.51710095239753	37.0	35.0	37.0	33.0	37.0
6	35.45156869314004	37.0	35.0	37.0	33.0	37.0
7	30.070261615638636	37.0	35.0	37.0	0.0	37.0
8	31.813614629243695	37.0	32.0	37.0	17.0	37.0
9	35.7496791260045	39.0	33.0	39.0	31.0	39.0
10	36.27140936560492	37.0	35.0	39.0	32.0	39.0
11	36.549266666956726	38.0	35.0	39.0	32.0	39.0
12	36.75763680109293	39.0	35.0	39.0	33.0	39.0
13	36.65357355737227	39.0	35.0	39.0	33.0	39.0
14	37.745954812239766	40.0	36.0	41.0	33.0	41.0
15	37.79805604744149	40.0	36.0	41.0	33.0	41.0
16	37.790037460679336	40.0	36.0	41.0	33.0	41.0
17	37.540451877602344	39.0	36.0	41.0	32.0	41.0
18	37.370273362889996	39.0	36.0	40.0	32.0	41.0
19	37.1480371213143	39.0	36.0	40.0	32.0	41.0
20	37.133348706279556	39.0	35.0	40.0	32.0	41.0
21	37.16946062712919	39.0	35.0	40.0	32.0	41.0
22	37.34324598309266	39.0	35.0	40.0	33.0	41.0
23	37.33868195839732	39.0	35.0	40.0	33.0	41.0
24	37.19320312737936	39.0	35.0	40.0	33.0	41.0
25	36.91071218799083	38.0	35.0	40.0	32.0	41.0
26	36.945910433734625	39.0	35.0	40.0	32.0	41.0
27	37.04968217158819	39.0	35.0	40.0	33.0	41.0
28	36.92366897115832	39.0	35.0	40.0	32.0	41.0
29	36.84625458469116	39.0	35.0	40.0	32.0	41.0
30	36.63623983536445	38.0	35.0	40.0	31.0	41.0
31	36.55816412215401	39.0	35.0	40.0	31.0	41.0
32	36.26983871458965	38.0	35.0	40.0	30.0	41.0
33	36.04530523274786	38.0	35.0	40.0	30.0	41.0
34	35.84725962730757	39.0	35.0	40.0	29.0	41.0
35	35.604805060889916	39.0	35.0	40.0	26.0	41.0
36	35.491309209409984	39.0	35.0	40.0	25.0	41.0
37	35.33835129502569	38.0	35.0	40.0	25.0	41.0
38	35.18334848873786	38.0	35.0	40.0	24.0	41.0
39	34.88941920719106	38.0	34.0	40.0	23.0	41.0
40	34.66847951409888	38.0	34.0	40.0	22.0	41.0
41	34.51468188878398	38.0	34.0	40.0	21.0	41.0
42	34.438855556667434	38.0	34.0	40.0	21.0	41.0
43	34.37652986194804	38.0	34.0	40.0	21.0	41.0
44	34.25323158183266	38.0	34.0	40.0	20.0	41.0
45	34.222140523231275	37.0	33.0	40.0	20.0	41.0
46	33.850957836069284	37.0	33.0	40.0	19.0	41.0
47	33.614359491996645	37.0	33.0	40.0	18.0	41.0
48	33.5678316749405	37.0	33.0	40.0	18.0	41.0
49	33.47565055842952	37.0	33.0	40.0	17.0	41.0
50	33.31889436610526	36.0	33.0	40.0	17.0	41.0
51	31.312437728690703	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	7.0
10	9.0
11	6.0
12	7.0
13	6.0
14	7.0
15	21.0
16	29.0
17	52.0
18	124.0
19	213.0
20	361.0
21	500.0
22	829.0
23	1263.0
24	1948.0
25	2940.0
26	4269.0
27	4947.0
28	5158.0
29	5315.0
30	5851.0
31	6681.0
32	8530.0
33	11572.0
34	18201.0
35	21140.0
36	22759.0
37	32637.0
38	40708.0
39	33740.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.34464695158827	14.999064570724979	18.40359204841608	20.25269642927067
2	34.37419781501125	27.779203884424454	21.293415883154008	16.553182417410298
3	28.760751998120437	27.70610987595773	29.21149838366523	14.321639742256604
4	25.237446756670916	21.66802267654596	35.75210689128572	17.342423675497407
5	26.48918165166354	24.830208709499175	28.53624897211551	20.144360666721777
6	22.292802415582948	33.88559917508191	29.946789302169762	13.87480910716539
7	58.856774900909755	19.789767708981426	17.434661352848273	3.9187960372605413
8	67.20950570176775	10.341061864506333	16.392201565430014	6.057230868295909
9	63.70490904581864	5.880151931117599	17.727037386715164	12.687901636348606
10	38.090244995453375	21.10197919431259	25.114753242458914	15.693022567775113
11	31.16850344368498	20.745645903037317	29.690090105768768	18.395760547508928
12	27.082200303688204	19.078841459965805	34.16274729051823	19.67621094582777
13	23.62067690272841	21.66845775992969	38.085894161616075	16.62497117572583
14	20.988857514542662	25.99579709451316	32.06085946371623	20.954485927227953
15	18.397935964427585	21.346496055969126	42.26182447866134	17.993743500941957
16	21.878167950887786	21.983893213134298	33.54579905238839	22.592139783589523
17	21.934728790772752	21.30690346804965	34.03265735878281	22.725710382394787
18	22.48075843735452	22.036538302565685	34.69224376851824	20.79045949156156
19	22.070474806496666	24.64007727080895	30.8548083240153	22.434639598679087
20	23.070296422309337	24.281568562615025	34.630026844644775	18.018108170430864
21	22.388955843387386	27.183139648713677	32.24141906796438	18.186485439934565
22	20.70300773143173	21.91515003850488	32.59688219247219	24.784960037591205
23	20.26922959785243	26.71586009458713	33.341309862035054	19.673600445525384
24	23.390082709351248	21.49268407290257	32.08348379967021	23.033749418075974
25	20.16654991929203	27.016937796128627	30.415809189831233	22.40070309474811
26	19.227204893817902	22.21492248989519	32.74002462571952	25.817847990567394
27	21.655840341801504	23.017651332877946	32.219229815394115	23.107278509926427
28	16.809446530427557	23.0041637479823	34.92066254497674	25.2657271766134
29	20.65079772538407	23.793840089453145	30.579835625497626	24.97552655966516
30	21.098063443859015	23.963087525724305	35.058583977619314	19.88026505279737
31	24.724483447252666	21.69195226265114	28.287381276621666	25.296183013474533
32	23.15426751536932	26.7254319290292	29.73229319399063	20.388007361610853
33	21.316040219107993	23.829516926919045	28.78903241806292	26.065410435910042
34	23.626332986716907	22.712657880882873	30.394055020644707	23.266954111755517
35	18.5737096514547	23.830387093686507	33.59496347474993	24.000939780108858
36	20.876606001540196	25.823504074555885	26.672786839597805	26.627103084306107
37	21.249037378013497	26.66365008853947	31.65927750053298	20.428035032914057
38	20.434996367053746	28.207325934015255	25.96795175795441	25.389725940976586
39	25.235706423135994	25.229615255763765	26.938622787057138	22.5960555340431
40	22.95935015945806	23.59587714985577	31.762827345860835	21.681945344825333
41	20.920114339913244	23.846920262268263	27.63780178471204	27.59516361310645
42	20.27009976461989	22.122684812544325	33.194251678334155	24.412963744501635
43	22.00477721555336	22.124860229462975	28.18209109775889	27.68827145722478
44	21.660626259022543	22.4376851823652	29.998129141449958	25.9035594171623
45	20.779147323584564	21.549679996171268	29.47689924774083	28.19427343250334
46	24.995975478700494	24.181934467740742	27.966724822812296	22.85536523074647
47	19.210671725236143	22.135302230672508	35.3922929329406	23.26173311115075
48	20.98755226439147	23.52756905861008	29.72707219338586	25.757806483612583
49	20.661239726593603	20.675162394872977	34.965476133500985	23.698121745032434
50	21.28862996593297	20.944914092785883	32.253166319325096	25.513289621956048
51	20.11956091384914	20.65297314230272	29.381615986703853	29.84584995714429
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	56.0
1	309.5
2	563.0
3	3396.0
4	6229.0
5	4168.5
6	2108.0
7	1870.0
8	1632.0
9	1643.5
10	1655.0
11	1580.5
12	1506.0
13	1451.5
14	1397.0
15	1314.0
16	1231.0
17	1193.0
18	1155.0
19	1123.5
20	1092.0
21	1052.5
22	1013.0
23	955.5
24	898.0
25	1031.5
26	1207.0
27	1249.0
28	1437.5
29	1626.0
30	1956.0
31	2286.0
32	2612.0
33	2938.0
34	3415.5
35	3893.0
36	4148.0
37	4403.0
38	4877.5
39	5352.0
40	6395.0
41	7438.0
42	8725.0
43	10012.0
44	12111.5
45	14211.0
46	21922.0
47	29633.0
48	25123.0
49	20613.0
50	20836.5
51	21060.0
52	18731.5
53	16403.0
54	14912.0
55	13421.0
56	12458.5
57	11496.0
58	10714.0
59	9932.0
60	9482.5
61	9033.0
62	8319.5
63	7606.0
64	6402.0
65	5198.0
66	4311.5
67	3425.0
68	2874.5
69	2324.0
70	2008.5
71	1693.0
72	1426.0
73	1159.0
74	924.0
75	577.5
76	466.0
77	372.5
78	279.0
79	213.0
80	147.0
81	105.0
82	63.0
83	53.0
84	43.0
85	32.0
86	21.0
87	18.5
88	16.0
89	9.0
90	2.0
91	3.0
92	4.0
93	3.0
94	2.0
95	3.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	229841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.75134301237724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.92117557529659	33.81993059623557
2	9.8906296735048	9.050191816018295
3	4.385100093343067	6.018726555424384
4	2.7707267384772862	5.070578776225583
5	1.8995616794419603	4.345374898465826
6	1.4353510116562411	3.9401541886468423
7	1.17083368514789	3.7497049477752773
8	0.880370561495531	3.2222508429584953
9	0.7885404606983535	3.2469106586893512
>10	2.793828762899736	17.302699921226054
>50	0.037929793835389435	1.1754638439427252
>100	0.01397413457093295	1.2339173684278337
>500	0.006987067285466475	1.8161692724787175
>1k	0.004990762346761767	6.007926313485041
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC	3086	1.3426673221922982	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	3062	1.3322253209827664	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG	2904	1.2634821463533488	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2756	1.1990898055612358	No Hit
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	1348	0.5864924012687032	TruSeq Adapter, Index 13 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCT	662	0.2880252000295857	No Hit
GAATCTATCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC	650	0.28280419942481977	RNA PCR Primer, Index 13 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT	584	0.2540886960986073	RNA PCR Primer, Index 13 (95% over 23bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG	534	0.23233452691208267	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	521	0.2266784429235863	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	517	0.22493810938866432	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTC	509	0.22145744231882042	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTA	483	0.2101452743418276	No Hit
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	355	0.15445460122432464	TruSeq Adapter, Index 13 (96% over 25bp)
GCTGTCACTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	277	0.12051809729334627	TruSeq Adapter, Index 13 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	267	0.11616726345604134	Illumina PCR Primer Index 4 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16620185258504794	0.0
2	0.0	0.0	0.0	1.3339656545176883	0.0
3	0.0	0.0	0.0	1.6028471856631323	0.0
4	0.0	0.0	0.0	2.1989114213739063	0.0
5	0.0	0.0	0.0	4.817243224664007	0.0
6	0.0	0.0	0.0	5.408956626537476	0.0
7	0.0	0.0	0.0	6.258674474963127	0.0
8	0.0	0.0	0.0	7.507798869653369	0.0
9	0.0	0.0	0.0	7.894152914406046	0.0
10	0.0	0.0	0.0	10.585578726162868	0.0
11	0.0	0.0	0.0	11.698086938361737	0.0
12	0.0	0.0	0.0	14.54831818518019	0.0
13	0.0	0.0	0.0	15.056930660761134	0.0
14	0.0	0.0	0.0	15.276647769545033	0.0
15	0.0	0.0	0.0	15.858789336976432	0.0
16	0.0	0.0	0.0	16.500537327978908	0.0
17	0.0	0.0	0.0	17.243659747390588	0.0
18	0.0	0.0	0.0	18.018543253814595	0.0
19	0.0	0.0	0.0	18.493654308848292	0.0
20	0.0	0.0	0.0	18.945705944544272	0.0
21	0.0	0.0	0.0	19.461714837648636	0.0
22	0.0	0.0	0.0	19.979899147671652	0.0
23	0.0	0.0	0.0	20.4671925374498	0.0
24	0.0	0.0	0.0	20.877911251691387	0.0
25	0.0	0.0	0.0	21.239900626955155	0.0
26	0.0	0.0	0.0	21.582746333334782	0.0
27	0.0	0.0	0.0	21.966924961168807	0.0
28	0.0	0.0	0.0	22.334135337037342	0.0
29	0.0	0.0	0.0	22.674370543114588	0.0
30	0.0	0.0	0.0	23.106843426542696	0.0
31	0.0	0.0	0.0	23.50059388881879	0.0
32	0.0	0.0	0.0	23.902610935385766	0.0
33	0.0	0.0	0.0	24.301147314882897	0.0
34	0.0	0.0	0.0	24.677494441809774	0.0
35	0.0	0.0	0.0	25.08125182191167	0.0
36	0.0	0.0	0.0	25.459774365757198	0.0
37	0.0	0.0	0.0	25.848303827428527	0.0
38	0.0	0.0	0.0	26.238573622634778	0.0
39	4.3508338373049194E-4	0.0	0.0	26.72717226256412	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTGAT	20	7.021496E-4	45.000004	13
CGGGTAT	35	1.2062628E-7	45.000004	6
GGTACCT	35	1.2062628E-7	45.000004	8
CTCGTTC	20	7.021496E-4	45.000004	15
TTTCGCA	20	7.021496E-4	45.000004	14
TAGCATA	40	6.7739165E-9	45.000004	30
AATGCGG	20	7.021496E-4	45.000004	2
CCCCGAT	20	7.021496E-4	45.000004	40
CCTCAAT	20	7.021496E-4	45.000004	38
TTCGCAA	20	7.021496E-4	45.000004	15
GGGTACC	50	2.1827873E-11	45.0	7
TGAAACG	50	2.1827873E-11	45.0	4
CGTTTTA	225	0.0	45.0	1
TGCGGGC	65	0.0	44.999996	4
CGTTAGG	30	2.1578999E-6	44.999996	2
CGTTTTT	1520	0.0	43.075657	1
TACGGCT	410	0.0	42.80488	7
ACGGCTG	470	0.0	42.12766	8
CGGTTTT	60	3.6379788E-12	41.249996	1
GTCAAGC	115	0.0	41.086956	16
>>END_MODULE