FastQCFastQC Report
Sat 18 Jun 2016
SRR3551686_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551686_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203198
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTC22701.117136979694682No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20421.0049311508971546No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGC18610.9158554710184156No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCG17550.8636896032441265No Hit
GCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC7560.37205090601285445TruSeq Adapter, Index 22 (95% over 21bp)
GAATCTATCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTC4330.21309264854969043No Hit
GAACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT4120.2027579011604445No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.1998051161920885No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCT3690.18159627555389324No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCG3080.15157629504227405No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTC2840.13976515516885007No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGC2830.13927302434079075No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATCATCCTCGTA2260.11122156714140888No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG351.2053351E-745.0000042
TACGGGA406.7666406E-945.04
ATCACGG207.019566E-445.02
GTTGTAG207.019566E-445.01
TACGAAA253.8794366E-545.020
GGGTTAC207.019566E-445.07
ATACGAA253.8794366E-545.019
ACATACG253.8794366E-545.017
TATCGGG207.019566E-445.03
AAGGACC253.8794366E-545.06
TACACGG253.8794366E-545.02
CGAAAAG302.1566557E-644.99999622
ACGGGAC302.1566557E-644.9999965
CGTTTTA2450.043.1632651
CGTTTTT11000.042.5454561
GTAGGGC451.9148501E-840.0000044
AGGCGAT451.9148501E-840.0000047
TAGGGCG403.439509E-739.3755
GGCGACT403.439509E-739.37510
CAATCTT403.439509E-739.37517
Produced by FastQC (version 0.11.5)