##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551686_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	203198
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.831140070276284	31.0	31.0	34.0	30.0	34.0
2	31.873561747654996	33.0	31.0	34.0	30.0	34.0
3	31.55714623175425	31.0	31.0	34.0	30.0	34.0
4	35.510472544021106	37.0	35.0	37.0	33.0	37.0
5	35.57011387907361	37.0	35.0	37.0	33.0	37.0
6	35.501186035295625	37.0	35.0	37.0	33.0	37.0
7	30.086708530595775	37.0	35.0	37.0	0.0	37.0
8	31.851932597761788	37.0	32.0	37.0	17.0	37.0
9	35.84279864959301	39.0	34.0	39.0	32.0	39.0
10	36.33143042746484	37.0	35.0	39.0	32.0	39.0
11	36.63144814417465	38.0	35.0	39.0	32.0	39.0
12	36.90404925245327	39.0	37.0	39.0	33.0	39.0
13	36.86552032992451	39.0	37.0	39.0	33.0	39.0
14	38.00621561235839	40.0	37.0	41.0	33.0	41.0
15	38.132988513666476	40.0	37.0	41.0	33.0	41.0
16	38.060625596708626	40.0	37.0	41.0	33.0	41.0
17	37.85974271400309	40.0	37.0	41.0	33.0	41.0
18	37.69566137461983	39.0	36.0	41.0	33.0	41.0
19	37.49874506638845	39.0	36.0	41.0	33.0	41.0
20	37.4921898837587	39.0	35.0	41.0	33.0	41.0
21	37.49025580960443	39.0	35.0	41.0	33.0	41.0
22	37.640961033081034	39.0	35.0	41.0	33.0	41.0
23	37.620744298664356	39.0	35.0	41.0	33.0	41.0
24	37.49365151231803	39.0	35.0	41.0	33.0	41.0
25	37.150838098800186	39.0	35.0	41.0	32.0	41.0
26	37.18837291705627	39.0	35.0	41.0	33.0	41.0
27	37.23796494059981	39.0	35.0	41.0	33.0	41.0
28	37.13873660173821	39.0	35.0	41.0	32.0	41.0
29	37.129204027598696	39.0	35.0	41.0	32.0	41.0
30	36.8853285957539	39.0	35.0	41.0	32.0	41.0
31	36.80931898936013	39.0	35.0	41.0	31.0	41.0
32	36.66218171438695	39.0	35.0	41.0	31.0	41.0
33	36.4309737300564	39.0	35.0	41.0	30.0	41.0
34	36.28325574070611	39.0	35.0	41.0	30.0	41.0
35	36.09570960344098	39.0	35.0	41.0	29.0	41.0
36	35.960831307394756	39.0	35.0	41.0	28.0	41.0
37	35.826489433951124	39.0	35.0	41.0	27.0	41.0
38	35.696842488607174	39.0	35.0	41.0	26.0	41.0
39	35.61686138643097	39.0	35.0	41.0	26.0	41.0
40	35.44338526954005	39.0	35.0	40.0	25.0	41.0
41	35.26107540428548	39.0	35.0	40.0	24.0	41.0
42	35.25507140818315	38.0	35.0	40.0	24.0	41.0
43	35.24814220612408	38.0	35.0	40.0	24.0	41.0
44	35.15702910461717	38.0	35.0	40.0	24.0	41.0
45	35.11303752989695	38.0	35.0	40.0	23.0	41.0
46	34.91314383015581	38.0	34.0	40.0	23.0	41.0
47	34.79351174716287	38.0	34.0	40.0	23.0	41.0
48	34.755780076575554	38.0	34.0	40.0	23.0	41.0
49	34.69434246400063	38.0	34.0	40.0	23.0	41.0
50	34.55570428842804	38.0	34.0	40.0	23.0	41.0
51	32.8101900609258	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	9.0
10	13.0
11	8.0
12	5.0
13	4.0
14	8.0
15	12.0
16	28.0
17	51.0
18	104.0
19	175.0
20	320.0
21	488.0
22	680.0
23	978.0
24	1472.0
25	2286.0
26	3169.0
27	3744.0
28	3902.0
29	3837.0
30	4069.0
31	4985.0
32	6342.0
33	8881.0
34	13925.0
35	17073.0
36	19604.0
37	29721.0
38	39113.0
39	38184.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.63773265484897	16.31807399679131	20.112402681128753	19.931790667230977
2	33.39304520713787	27.033730646955185	22.72512524729574	16.84809889861121
3	30.2778570655223	26.154784988041218	28.76258624592762	14.804771700508862
4	26.88609139853739	22.547466018366322	32.49982775421018	18.06661482888611
5	26.29405801238201	26.32752290869005	27.44367562672861	19.93474345219933
6	23.280248821346667	34.72130631207	28.278821641945296	13.719623224638038
7	61.19154716089725	20.9007962676798	14.268841228752253	3.6388153426706955
8	71.75464325436273	9.260917922420496	14.147777045049656	4.836661778167108
9	67.43078179903345	6.989734150926682	15.460289963483891	10.11919408655597
10	38.14210769791041	24.231537711985354	22.846189430998336	14.780165159105895
11	29.360033071191644	23.15721611433183	29.26062264392366	18.22212817055286
12	26.704987253811552	20.387503814013918	33.11056211183181	19.79694682034272
13	23.163613815096607	22.220691148534925	36.90931997362179	17.70637506274668
14	20.970186714436164	26.535202118131085	31.915176330475692	20.579434836957056
15	19.068101063986852	23.55682634671601	38.53581236035788	18.83926022893926
16	21.327473695607242	23.57306666404197	33.70702467543972	21.39243496491107
17	21.922951997559032	21.973641472849142	33.5111566058721	22.59224992371972
18	22.35209007962677	23.044518154706246	33.09629031781809	21.507101447848896
19	22.641463006525655	24.481540172639495	30.904831740469884	21.972165080364963
20	25.025344737645057	23.794033405840608	32.340377365918954	18.840244490595378
21	23.891967440624416	25.695134794633805	31.157294855264322	19.255602909477457
22	22.000216537564345	22.254648175671022	32.33693245012254	23.408202836642094
23	22.01153554660971	26.322601600409453	32.06872114883021	19.59714170415063
24	23.51253457219067	22.56173781238004	31.26753216074961	22.65819545467967
25	20.695085581550998	27.038651955235782	30.704534493449735	21.56172796976348
26	20.842232699140737	23.838817311194006	31.37973798954714	23.93921200011811
27	22.028760125591788	25.80340357680686	31.031309363281135	21.13652693432022
28	19.16406657545842	24.298959635429483	34.42701207689052	22.109961712221576
29	21.238398015728503	23.709386903414405	30.448626462858886	24.60358861799821
30	20.902764790992038	26.761582299038377	31.84726227620351	20.48839063376608
31	22.676896426145927	23.95496018661601	31.055423773856045	22.312719613382022
32	23.208889851278062	25.23745312453863	28.885618952942448	22.668038071240858
33	21.201488203624052	26.11590665262453	28.66612860362799	24.016476540123428
34	21.314678294077698	24.388035315308223	31.576098189942815	22.721188200671268
35	19.80678943690391	25.50172737920649	28.796543273063712	25.894939910825894
36	21.148338074193642	26.505674268447528	29.013572968237877	23.332414689120956
37	20.529729623323064	27.43334087933936	29.494384787251843	22.54254471008573
38	20.602564985875844	27.586393566865812	27.790627860510437	24.020413586747903
39	22.322070099115148	24.3895117077924	29.168594179076567	24.119824014015887
40	21.5784604179175	25.39542712034567	30.383665193555053	22.642447268181773
41	18.97361194499946	26.007637870451482	29.564759495664326	25.45399068888473
42	20.600596462563608	24.452012322955934	30.264569533164696	24.68282168131576
43	22.63112825913641	24.36736582052973	27.786198683057904	25.215307237275958
44	21.187216409610333	24.688235120424416	29.780312798354313	24.344235671610942
45	20.423429364462248	24.304373074538134	28.84772487918188	26.424472681817733
46	23.417061191547162	25.547495546216005	28.02685065797892	23.008592604257917
47	20.050886327621335	24.608509926278803	33.46588056969065	21.874723176409216
48	21.133082018523805	24.61835254283999	29.35068258545852	24.897882853177688
49	19.949015246213055	23.657221035640113	32.80888591423144	23.584877803915393
50	20.51348930599711	23.885569739859644	31.60710243211055	23.9938385220327
51	20.019882085453595	24.09570960344098	28.72518430299511	27.159224008110318
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	75.0
1	266.0
2	457.0
3	2541.5
4	4626.0
5	3157.0
6	1688.0
7	1584.0
8	1480.0
9	1451.0
10	1422.0
11	1349.5
12	1277.0
13	1188.5
14	1100.0
15	1054.0
16	1008.0
17	1005.0
18	1002.0
19	937.5
20	873.0
21	899.0
22	925.0
23	945.0
24	965.0
25	1099.5
26	1427.5
27	1621.0
28	1944.5
29	2268.0
30	2664.0
31	3060.0
32	3416.5
33	3773.0
34	4000.0
35	4227.0
36	4831.0
37	5435.0
38	5958.5
39	6482.0
40	7273.5
41	8065.0
42	8927.5
43	9790.0
44	11213.5
45	12637.0
46	17497.0
47	22357.0
48	19433.5
49	16510.0
50	16327.5
51	16145.0
52	14663.0
53	13181.0
54	12242.5
55	11304.0
56	10712.5
57	10121.0
58	9646.0
59	9171.0
60	8617.5
61	8064.0
62	7370.0
63	6676.0
64	5741.5
65	4807.0
66	3911.0
67	3015.0
68	2632.5
69	2250.0
70	1906.0
71	1562.0
72	1289.5
73	1017.0
74	853.5
75	523.5
76	357.0
77	301.0
78	245.0
79	180.0
80	115.0
81	78.5
82	42.0
83	36.0
84	30.0
85	27.0
86	24.0
87	18.0
88	12.0
89	10.0
90	8.0
91	4.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	203198.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.57428714849556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.16333919520017	34.80693707615232
2	9.726905968759699	9.255012352483783
3	4.693286438398676	6.698392700715558
4	3.0567911451329266	5.816986387661296
5	2.142339919313127	5.096014724554376
6	1.6447708699700012	4.694928099686021
7	1.2361642701975795	4.116674376716306
8	1.04375711182373	3.972480044094922
9	0.7696286334953968	3.2953080246852826
>10	2.4785352229233477	15.505565999665352
>50	0.026895624288817626	0.8523705941987617
>100	0.012413365056377365	1.6156655085187845
>500	0.0010344470880314471	0.37205090601285445
>1k	0.0041377883521257885	3.901613204854378
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTC	2270	1.117136979694682	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2042	1.0049311508971546	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGC	1861	0.9158554710184156	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCG	1755	0.8636896032441265	No Hit
GCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC	756	0.37205090601285445	TruSeq Adapter, Index 22 (95% over 21bp)
GAATCTATCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTC	433	0.21309264854969043	No Hit
GAACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT	412	0.2027579011604445	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	406	0.1998051161920885	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCT	369	0.18159627555389324	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCG	308	0.15157629504227405	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTC	284	0.13976515516885007	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGC	283	0.13927302434079075	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATCATCCTCGTA	226	0.11122156714140888	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08710715656650164	0.0
2	0.0	0.0	0.0	0.7534522977588362	0.0
3	0.0	0.0	0.0	0.9143790785342375	0.0
4	0.0	0.0	0.0	1.3061152176694653	0.0
5	0.0	0.0	0.0	3.040876386578608	0.0
6	0.0	0.0	0.0	3.4532820204923276	0.0
7	0.0	0.0	0.0	3.948365633520015	0.0
8	0.0	0.0	0.0	4.670321558283054	0.0
9	0.0	0.0	0.0	4.906544355751533	0.0
10	0.0	0.0	0.0	6.526146910894792	0.0
11	0.0	0.0	0.0	7.177728127245347	0.0
12	0.0	0.0	0.0	8.958257463164008	0.0
13	0.0	0.0	0.0	9.28109528637093	0.0
14	0.0	0.0	0.0	9.418399787399482	0.0
15	0.0	0.0	0.0	9.76682841366549	0.0
16	0.0	0.0	0.0	10.15659602948848	0.0
17	0.0	0.0	0.0	10.59754525142964	0.0
18	0.0	0.0	0.0	11.10493213515881	0.0
19	0.0	0.0	0.0	11.423832911741258	0.0
20	0.0	0.0	0.0	11.690075689721356	0.0
21	0.0	0.0	0.0	12.014882036240515	0.0
22	0.0	0.0	0.0	12.364294924162639	0.0
23	0.0	0.0	0.0	12.689101270681798	0.0
24	0.0	0.0	0.0	12.951899132865481	0.0
25	0.0	0.0	0.0	13.214696995049163	0.0
26	0.0	0.0	0.0	13.445506353408991	0.0
27	0.0	0.0	0.0	13.709288477248792	0.0
28	0.0	0.0	0.0	13.977499778541127	0.0
29	0.0	0.0	0.0	14.23291567830392	0.0
30	0.0	0.0	0.0	14.54984793157413	0.0
31	0.0	0.0	0.0	14.815598578726169	0.0
32	0.0	0.0	0.0	15.099558066516403	0.0
33	0.0	0.0	0.0	15.378104115197985	0.0
34	0.0	0.0	0.0	15.642870500693904	0.0
35	0.0	0.0	0.0	15.923877203515783	0.0
36	0.0	0.0	0.0	16.207344560477956	0.0
37	0.0	0.0	0.0	16.480477170050886	0.0
38	0.0	0.0	0.0	16.762468134528884	0.0
39	0.0	0.0	0.0	17.097117097609228	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	35	1.2053351E-7	45.000004	2
TACGGGA	40	6.7666406E-9	45.0	4
ATCACGG	20	7.019566E-4	45.0	2
GTTGTAG	20	7.019566E-4	45.0	1
TACGAAA	25	3.8794366E-5	45.0	20
GGGTTAC	20	7.019566E-4	45.0	7
ATACGAA	25	3.8794366E-5	45.0	19
ACATACG	25	3.8794366E-5	45.0	17
TATCGGG	20	7.019566E-4	45.0	3
AAGGACC	25	3.8794366E-5	45.0	6
TACACGG	25	3.8794366E-5	45.0	2
CGAAAAG	30	2.1566557E-6	44.999996	22
ACGGGAC	30	2.1566557E-6	44.999996	5
CGTTTTA	245	0.0	43.163265	1
CGTTTTT	1100	0.0	42.545456	1
GTAGGGC	45	1.9148501E-8	40.000004	4
AGGCGAT	45	1.9148501E-8	40.000004	7
TAGGGCG	40	3.439509E-7	39.375	5
GGCGACT	40	3.439509E-7	39.375	10
CAATCTT	40	3.439509E-7	39.375	17
>>END_MODULE