##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551685_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	211813
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.173025262849777	31.0	31.0	33.0	28.0	34.0
2	31.211323195460146	31.0	31.0	34.0	28.0	34.0
3	31.200842252364115	31.0	31.0	34.0	28.0	34.0
4	35.13282942973283	37.0	35.0	37.0	32.0	37.0
5	32.901361106258825	35.0	35.0	37.0	28.0	37.0
6	33.918734921841434	35.0	35.0	37.0	28.0	37.0
7	35.09235977017463	37.0	35.0	37.0	32.0	37.0
8	34.48490885828537	36.0	35.0	37.0	31.0	37.0
9	36.17159947689707	39.0	35.0	39.0	31.0	39.0
10	35.892135987876095	37.0	35.0	39.0	31.0	39.0
11	36.26244848049931	37.0	35.0	39.0	32.0	39.0
12	36.38560900416877	38.0	35.0	39.0	32.0	39.0
13	36.3715541538999	39.0	35.0	39.0	32.0	39.0
14	37.31443301402653	39.0	36.0	41.0	32.0	41.0
15	37.52394801074533	39.0	36.0	41.0	32.0	41.0
16	37.66872665983674	39.0	36.0	41.0	33.0	41.0
17	37.48221780532828	39.0	36.0	41.0	32.0	41.0
18	37.44455250622011	39.0	36.0	40.0	32.0	41.0
19	37.276484446186025	39.0	36.0	40.0	32.0	41.0
20	36.99102510233083	39.0	35.0	40.0	31.0	41.0
21	37.09571178350715	39.0	35.0	40.0	32.0	41.0
22	37.18757583340022	39.0	35.0	41.0	32.0	41.0
23	37.08729870215709	39.0	35.0	41.0	31.0	41.0
24	37.06647845033119	39.0	35.0	41.0	32.0	41.0
25	36.848248218947845	39.0	35.0	40.0	31.0	41.0
26	36.846203962929565	39.0	35.0	40.0	31.0	41.0
27	36.63809114643577	39.0	35.0	40.0	31.0	41.0
28	36.558266017666526	39.0	35.0	40.0	30.0	41.0
29	36.686416792170455	39.0	35.0	41.0	31.0	41.0
30	36.31939493798775	39.0	35.0	40.0	30.0	41.0
31	36.2676370194464	38.0	35.0	40.0	30.0	41.0
32	35.861727089460985	39.0	35.0	40.0	27.0	41.0
33	35.74126706103969	39.0	35.0	41.0	26.0	41.0
34	35.59701245910308	39.0	35.0	41.0	25.0	41.0
35	35.32159027066327	39.0	35.0	41.0	23.0	41.0
36	35.142824094838375	39.0	35.0	41.0	23.0	41.0
37	35.08300718086237	39.0	35.0	41.0	22.0	41.0
38	34.946046748783125	39.0	35.0	40.0	21.0	41.0
39	34.820181008719956	39.0	35.0	40.0	21.0	41.0
40	34.74201772318035	38.0	34.0	40.0	21.0	41.0
41	34.65678688276923	38.0	34.0	40.0	21.0	41.0
42	34.70092959355658	38.0	34.0	40.0	21.0	41.0
43	34.649657953005715	38.0	34.0	40.0	20.0	41.0
44	34.79019229225779	38.0	35.0	40.0	21.0	41.0
45	34.79596625325169	38.0	35.0	40.0	21.0	41.0
46	34.64009763328974	38.0	34.0	40.0	20.0	41.0
47	34.547973920392046	38.0	34.0	40.0	20.0	41.0
48	34.58447781769769	38.0	34.0	40.0	20.0	41.0
49	34.52338147328068	38.0	34.0	40.0	20.0	41.0
50	34.39854494294496	38.0	34.0	40.0	20.0	41.0
51	33.69287059812193	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	8.0
10	6.0
11	13.0
12	13.0
13	15.0
14	18.0
15	24.0
16	40.0
17	89.0
18	106.0
19	289.0
20	593.0
21	1172.0
22	1873.0
23	2332.0
24	3051.0
25	3546.0
26	4003.0
27	3858.0
28	3748.0
29	3669.0
30	4136.0
31	4933.0
32	6432.0
33	8756.0
34	14329.0
35	16244.0
36	18516.0
37	26750.0
38	40932.0
39	42312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.48723166189044	15.711028123863974	19.000250220713554	18.80148999353203
2	32.15383380623474	29.771543767379715	21.622374452937258	16.452247973448276
3	29.191787095220782	29.395740582494938	26.798166307072748	14.61430601521153
4	26.06119548847332	21.962296931727515	34.491745077025485	17.484762502773673
5	28.53696420899567	29.888156062186926	23.90693677914009	17.66794294967731
6	22.670468762540544	38.06423590620028	25.656121201248272	13.609174130010906
7	69.7147955980039	5.786235972296318	20.384017978122213	4.114950451577571
8	70.17510728803236	12.19471892660035	12.661640220383074	4.968533564984208
9	64.75995335508208	6.947637774829685	14.094980005948642	14.197428864139594
10	41.696213169163364	23.1482486910624	20.959525619296265	14.196012520477968
11	33.33175961815375	22.277197339162374	27.02383706382517	17.36720597885871
12	25.61032608952236	19.732027779220353	30.441946433882716	24.21569969737457
13	27.97562000443788	21.08321963241161	33.87421924055653	17.066941122593988
14	20.249465330267736	30.09683069499983	29.823004253752128	19.8306997209803
15	18.562600029271103	22.573685278996095	35.39206753126579	23.471647160467015
16	20.65123481561566	22.302691525071644	31.552359864597545	25.493713794715152
17	21.20172038543432	26.31755369122764	31.23887580082431	21.24185012251373
18	21.630400400353142	21.816413534580033	30.730880540854432	25.822305524212396
19	21.93019314206399	23.427268392402734	29.104918017307718	25.537620448225557
20	28.527521917918165	22.545358405763576	30.707746927714542	18.21937274860372
21	23.23983891451422	28.919849112188583	29.360331990954286	18.479979982342915
22	21.547780353424955	21.35326915722831	35.14137470315797	21.95757578618876
23	25.556977144934446	24.989495451176275	30.113826818939348	19.33970058494993
24	21.944356578680253	26.234933644299453	30.179922856481895	21.6407869205384
25	20.12813188992177	29.93489540302059	29.033628719672542	20.9033439873851
26	19.881215978244963	22.892834717415834	34.515822919273134	22.710126385066072
27	25.446030224773743	24.502745346130787	29.723388082884433	20.327836346211043
28	18.547492363547093	28.413742310434202	32.00653406542563	21.032231260593072
29	20.628573317029645	22.628922681799512	33.63485716174173	23.10764683942912
30	20.132853035460524	30.161038274326884	29.998158753239885	19.707949936972707
31	21.66014361724729	23.23747834174484	28.991610524377638	26.110767516630233
32	26.679193439496157	24.381884020338696	27.533720782010544	21.4052017581546
33	24.878548531015564	24.953614745081744	26.82271626387427	23.345120460028422
34	20.519986969638314	24.0542365199492	29.297068640734985	26.1287078696775
35	23.23228508165221	24.293126484210127	27.96004022415999	24.51454820997767
36	20.574752257887855	29.384881947755805	27.080490810290208	22.959874984066133
37	19.942590870248758	26.76228560097822	28.54687861462705	24.748244914145967
38	20.352858417566438	26.453050568189866	26.29111527621062	26.902975738033074
39	21.25270875725286	25.823249753320145	27.66024748244914	25.263794006977854
40	20.62054736961376	24.8242553573199	28.54074112542667	26.014456147639663
41	18.544187561669965	24.7572150906696	27.875059604462425	28.823537743198006
42	20.662093450354792	24.167544012879286	28.536492094441794	26.633870442324124
43	21.59593603792024	24.674595043741412	26.647089649832633	27.08237926850571
44	20.731966404328347	24.559399092595825	30.014682762625522	24.693951740450302
45	20.386850665445465	23.347953147351674	29.170541940296395	27.09465424690647
46	23.634526681554014	25.64431833740139	27.03894472954918	23.682210251495423
47	19.289656442239146	27.73153678008432	31.557553124690173	21.421253652986362
48	20.70505587475745	24.78837465122537	29.594972924230333	24.91159654978684
49	20.58561089262699	23.039190229117192	32.336542138584505	24.038656739671314
50	20.857076761105315	24.495663627822655	30.015626991733274	24.631632619338756
51	19.649879846846037	23.67654487684892	28.914655852095954	27.75891942420909
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	72.0
1	213.5
2	355.0
3	2160.5
4	3966.0
5	2743.5
6	1521.0
7	1404.0
8	1287.0
9	1290.5
10	1294.0
11	1232.0
12	1170.0
13	1130.5
14	1091.0
15	1013.5
16	936.0
17	937.5
18	939.0
19	883.0
20	827.0
21	906.0
22	985.0
23	961.0
24	937.0
25	1071.5
26	1334.0
27	1462.0
28	1841.5
29	2221.0
30	2565.5
31	2910.0
32	3261.5
33	3613.0
34	3830.0
35	4047.0
36	4692.0
37	5337.0
38	5761.5
39	6186.0
40	6981.0
41	7776.0
42	8610.0
43	9444.0
44	10744.0
45	12044.0
46	16683.5
47	21323.0
48	19569.0
49	17815.0
50	17699.0
51	17583.0
52	16237.5
53	14892.0
54	14185.0
55	13478.0
56	13073.0
57	12668.0
58	11798.5
59	10929.0
60	10036.5
61	9144.0
62	8091.0
63	7038.0
64	6201.5
65	5365.0
66	4346.5
67	3328.0
68	2820.5
69	2313.0
70	1934.0
71	1555.0
72	1346.0
73	1137.0
74	918.5
75	560.0
76	420.0
77	343.5
78	267.0
79	188.5
80	110.0
81	89.0
82	68.0
83	47.5
84	27.0
85	22.5
86	18.0
87	12.0
88	6.0
89	3.0
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	211813.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.23382889624338
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.82195041251748	32.65427523334262
2	8.32292700628649	7.363098582239995
3	3.980019852070059	5.281545514203566
4	2.684298720288602	4.749472411986044
5	2.1698526037163925	4.799044440142956
6	1.826176982271888	4.846728010084367
7	1.4526165241800348	4.497835354770482
8	1.272240188701397	4.502084385755359
9	0.9990073964970703	3.9770930018459683
>10	3.412207955770442	20.224915373466217
>50	0.03415409902554086	1.1259932109927153
>100	0.018144365107318584	1.5636434024351669
>500	0.002134631189096304	0.7275285275219179
>1k	0.004269262378192608	3.686742551212626
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTC	2124	1.0027713124312483	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2025	0.9560319715975883	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGC	1875	0.8852147885162855	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCG	1785	0.8427244786675038	No Hit
GCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC	829	0.3913829651626671	TruSeq Adapter, Index 22 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATCATCCT	712	0.33614556235925086	No Hit
GAACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT	427	0.20159291450477543	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATCATCCTCGTA	330	0.15579780277886626	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCT	323	0.1524930009017388	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTC	283	0.1336084187467247	No Hit
CCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC	274	0.12935938776184652	TruSeq Adapter, Index 22 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCCCTCGAATGC	228	0.10764211828358033	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.442291077507046E-4	0.0	0.0	0.07837101594330849	0.0
2	9.442291077507046E-4	0.0	0.0	0.7176141218905355	0.0
3	9.442291077507046E-4	0.0	0.0	0.8639696335918947	0.0
4	9.442291077507046E-4	0.0	0.0	1.2397728184766752	0.0
5	9.442291077507046E-4	0.0	0.0	2.72032405942978	0.0
6	9.442291077507046E-4	0.0	0.0	3.1990482170593872	0.0
7	9.442291077507046E-4	0.0	0.0	3.6909915821975043	0.0
8	9.442291077507046E-4	0.0	0.0	4.3212645116210995	0.0
9	9.442291077507046E-4	0.0	0.0	4.539853550065388	0.0
10	9.442291077507046E-4	0.0	0.0	5.929758796674425	0.0
11	9.442291077507046E-4	0.0	0.0	6.592135515761544	0.0
12	9.442291077507046E-4	0.0	0.0	8.143503939795952	0.0
13	9.442291077507046E-4	0.0	0.0	8.478705273047453	0.0
14	9.442291077507046E-4	0.0	0.0	8.620811753763933	0.0
15	9.442291077507046E-4	0.0	0.0	8.978202471047576	0.0
16	9.442291077507046E-4	0.0	0.0	9.404521913197017	0.0
17	9.442291077507046E-4	0.0	0.0	9.835562500885215	0.0
18	9.442291077507046E-4	0.0	0.0	10.286431899836176	0.0
19	9.442291077507046E-4	0.0	0.0	10.96061148277018	0.0
20	9.442291077507046E-4	0.0	0.0	11.280705150297669	0.0
21	9.442291077507046E-4	0.0	0.0	11.614962254441417	0.0
22	9.442291077507046E-4	0.0	0.0	11.95394050412392	0.0
23	9.442291077507046E-4	0.0	0.0	12.289141837375421	0.0
24	9.442291077507046E-4	0.0	0.0	12.553525987545617	0.0
25	9.442291077507046E-4	0.0	0.0	12.785334233498416	0.0
26	9.442291077507046E-4	0.0	0.0	13.026584770528721	0.0
27	9.442291077507046E-4	0.0	0.0	13.344317865286833	0.0
28	9.442291077507046E-4	0.0	0.0	13.588873204194266	0.0
29	9.442291077507046E-4	0.0	0.0	13.846175636056334	0.0
30	9.442291077507046E-4	0.0	0.0	14.158715470721816	0.0
31	9.442291077507046E-4	0.0	0.0	14.416962131691633	0.0
32	9.442291077507046E-4	0.0	0.0	14.697398176693593	0.0
33	9.442291077507046E-4	0.0	0.0	14.932983339077394	0.0
34	9.442291077507046E-4	0.0	0.0	15.20256074934022	0.0
35	9.442291077507046E-4	0.0	0.0	15.489134283542558	0.0
36	9.442291077507046E-4	0.0	0.0	15.76532129755964	0.0
37	9.442291077507046E-4	0.0	0.0	16.044340998899973	0.0
38	9.442291077507046E-4	0.0	0.0	16.31863955470155	0.0
39	9.442291077507046E-4	0.0	0.0	16.63778899312129	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGTCC	20	7.020245E-4	45.000004	6
ATCGTGA	20	7.020245E-4	45.000004	30
AAACGAT	20	7.020245E-4	45.000004	16
ACGGCTA	20	7.020245E-4	45.000004	30
CGAATGC	170	0.0	45.000004	45
ATACGGT	20	7.020245E-4	45.000004	28
ACGACCT	20	7.020245E-4	45.000004	23
CGGCGAT	50	2.1827873E-11	45.0	31
AACGATG	25	3.8800004E-5	45.0	17
CCGACGT	25	3.8800004E-5	45.0	44
CGTAAGC	50	2.1827873E-11	45.0	43
GCTACGA	35	1.2056626E-7	45.0	10
AATACGG	55	1.8189894E-12	45.0	5
CCCCTCG	125	0.0	44.999996	40
CCCTCGA	185	0.0	43.783783	41
TCGAATG	185	0.0	43.783783	44
CGTTTTT	1045	0.0	43.708134	1
ACGGGAG	100	0.0	42.75	5
CCGATGA	955	0.0	42.643974	18
CTCGAAT	190	0.0	42.63158	43
>>END_MODULE