FastQCFastQC Report
Sat 18 Jun 2016
SRR3551683_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551683_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411831
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC18150.4407147592094815No Hit
GCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC16930.41109095721303157No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTC13630.3309610009931258No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13560.32926127464906724No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCC12590.30570792388139795No Hit
CTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGCT12580.30546510583224673No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCG10590.25714431405115207No Hit
TCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC10040.24378932134783443No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGC8760.21270861105647706No Hit
ACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC8020.19474007541928606No Hit
TGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGCTT7720.18745553394474918Illumina Single End Adapter 2 (95% over 21bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA5560.1350068353280836No Hit
CGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG5520.13403556313147869No Hit
ACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG5520.13403556313147869No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5320.1291792021484541No Hit
GCCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG4730.11485293724853154No Hit
AGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG4310.10465457918417992No Hit
GGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG4220.10246921674181884No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGG207.028007E-445.0000042
TGACGGT207.028007E-445.0000043
ATCGTGA207.028007E-445.00000429
GGTATCC207.028007E-445.0000047
TTCGAAG207.028007E-445.0000041
TCGCGGA207.028007E-445.0000043
AATCGTT351.2093915E-745.00000421
TCACGTC207.028007E-445.00000438
CCCGTAT253.8864306E-545.05
ATAAGCG253.8864306E-545.01
GTATACG253.8864306E-545.01
GCAATCG253.8864306E-545.045
CGGTATG253.8864306E-545.06
CGGACCC253.8864306E-545.06
CACGGTT302.162098E-644.9999964
TACGGAA302.162098E-644.9999964
GTATGCG302.162098E-644.9999961
TGATTAT302.162098E-644.99999627
CGAATAT302.162098E-644.99999614
ACGGTAA302.162098E-644.9999965
Produced by FastQC (version 0.11.5)