##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551683_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	411831
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41849447953165	34.0	31.0	34.0	30.0	34.0
2	32.556980897504076	34.0	31.0	34.0	31.0	34.0
3	32.6044639670156	34.0	31.0	34.0	31.0	34.0
4	36.06853539437294	37.0	35.0	37.0	35.0	37.0
5	36.07770906026987	37.0	35.0	37.0	35.0	37.0
6	36.11582906580612	37.0	36.0	37.0	35.0	37.0
7	36.22430317290345	37.0	37.0	37.0	35.0	37.0
8	35.91768225315724	37.0	36.0	37.0	35.0	37.0
9	37.63978185226464	39.0	37.0	39.0	35.0	39.0
10	37.711104797841834	39.0	37.0	39.0	35.0	39.0
11	37.75571775801239	39.0	37.0	39.0	35.0	39.0
12	37.73995401026149	39.0	37.0	39.0	35.0	39.0
13	37.68805893679689	39.0	37.0	39.0	35.0	39.0
14	39.07579565404256	40.0	39.0	41.0	35.0	41.0
15	39.0926059475853	40.0	39.0	41.0	35.0	41.0
16	39.109510940167205	40.0	39.0	41.0	35.0	41.0
17	39.07853221345649	40.0	38.0	41.0	35.0	41.0
18	38.99398782510301	40.0	38.0	41.0	35.0	41.0
19	38.988735670699874	40.0	38.0	41.0	35.0	41.0
20	38.86774672134929	40.0	38.0	41.0	35.0	41.0
21	38.87029873904587	40.0	38.0	41.0	35.0	41.0
22	38.846480716604624	40.0	38.0	41.0	35.0	41.0
23	38.8615937119838	40.0	38.0	41.0	35.0	41.0
24	38.800670663451754	40.0	38.0	41.0	35.0	41.0
25	38.77014115013197	40.0	38.0	41.0	35.0	41.0
26	38.720795666183456	40.0	38.0	41.0	35.0	41.0
27	38.6183653003295	40.0	38.0	41.0	35.0	41.0
28	38.548212737749225	40.0	38.0	41.0	35.0	41.0
29	38.51301383334426	40.0	38.0	41.0	35.0	41.0
30	38.356957101335254	40.0	38.0	41.0	34.0	41.0
31	38.30113566001588	40.0	38.0	41.0	34.0	41.0
32	38.16828747714475	40.0	38.0	41.0	34.0	41.0
33	38.078379238085525	40.0	38.0	41.0	34.0	41.0
34	37.98797322202554	40.0	38.0	41.0	34.0	41.0
35	37.92641399020472	40.0	38.0	41.0	34.0	41.0
36	37.8367340001117	40.0	37.0	41.0	33.0	41.0
37	37.7884132083306	40.0	37.0	41.0	33.0	41.0
38	37.76153810665054	40.0	37.0	41.0	33.0	41.0
39	37.713212458508465	40.0	37.0	41.0	33.0	41.0
40	37.646787152982654	40.0	37.0	41.0	33.0	41.0
41	37.597849603356714	40.0	37.0	41.0	33.0	41.0
42	37.50225942194735	40.0	37.0	41.0	33.0	41.0
43	37.481202240724954	40.0	36.0	41.0	33.0	41.0
44	37.40829854964779	40.0	36.0	41.0	33.0	41.0
45	37.3642100764634	40.0	36.0	41.0	33.0	41.0
46	37.27944715186569	40.0	36.0	41.0	33.0	41.0
47	37.17292530188354	40.0	35.0	41.0	33.0	41.0
48	37.16515026794972	40.0	35.0	41.0	33.0	41.0
49	37.11556925049353	40.0	35.0	41.0	33.0	41.0
50	37.00681104627869	39.0	35.0	41.0	32.0	41.0
51	36.30812153528996	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	4.0
13	5.0
14	4.0
15	3.0
16	12.0
17	35.0
18	64.0
19	131.0
20	282.0
21	453.0
22	872.0
23	1245.0
24	1843.0
25	2422.0
26	2666.0
27	2855.0
28	2912.0
29	3149.0
30	3864.0
31	4818.0
32	6453.0
33	8883.0
34	15976.0
35	21907.0
36	25398.0
37	38449.0
38	72135.0
39	194436.0
40	554.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.771318817670355	23.859787145698114	24.232998487243552	14.135895549387978
2	32.89261857412385	27.49623024978693	21.7239595853639	17.887191590725322
3	31.49714324565174	27.239328753784925	24.270392466812844	16.993135533750493
4	29.130395720574704	28.525778778188137	22.72752658250593	19.616298918731225
5	29.12165427080526	31.168367607100972	18.855307152691275	20.8546709694025
6	39.93992681463999	27.30926035194048	16.77484210756354	15.975970725855996
7	76.39929971274626	5.698453977481054	11.242475675701927	6.659770634070772
8	66.54598609623851	8.452496290954299	15.47940781534173	9.522109797465465
9	34.1759119638881	25.923497745434414	19.493675803909856	20.40691448676763
10	25.857936872163584	26.613101005023903	28.687495598922858	18.841466523889654
11	24.84222897256399	19.61289946604311	32.53616167796985	23.008709883423055
12	24.285690003909373	20.346938428627276	33.91148310836241	21.45588845910094
13	20.00990697640537	26.73888075448424	32.13672598711608	21.114486281994314
14	17.281117740043854	24.634376722490536	36.85905140700918	21.225454130456427
15	18.751623845703698	24.346637334246328	34.00448242118733	22.897256398862638
16	18.54207186928619	24.176421881791317	29.801059172330397	27.480447076592096
17	20.216787954282218	23.8080669012289	29.593206922256943	26.381938222231938
18	20.891336494824333	26.902540119612173	31.540850494498958	20.665272891064536
19	23.42635692796317	22.606117557930318	29.1792021484541	24.788323365652413
20	19.705170324720577	28.83731433524917	29.790860814266047	21.66665452576421
21	18.942236014287413	26.798856812624592	28.55515976213544	25.70374741095255
22	17.956637552782574	27.99255034225204	28.215457311372873	25.83535479359252
23	18.49909307458642	24.087307657752817	30.179612510957167	27.233986756703597
24	20.361993147674653	26.417632475457165	28.559287668971013	24.66108670789717
25	18.130252457925703	27.499386884425892	28.763497648307197	25.606863009341215
26	19.912051302597426	27.387447763767174	27.616182366067633	25.084318567567763
27	22.25306011446443	24.798036087618463	31.486702069538232	21.462201728378876
28	19.548552683018034	25.892174217093906	28.179034604000186	26.380238495887877
29	22.93683574087429	23.117978005541108	27.68417141982998	26.26101483375462
30	22.497577889959715	25.345105152356183	27.96462626659965	24.19269069108445
31	25.157406800362285	23.900580577955523	26.769961464775598	24.172051156906598
32	21.78126464496359	25.095002561730418	26.843778151717572	26.27995464158842
33	21.33496507062363	21.247307754880033	32.75639764855001	24.661329525946325
34	23.14371671875114	20.336740070562925	29.89915766418749	26.620385546498444
35	23.010895245865413	20.412984937996413	30.783258181147122	25.792861634991056
36	24.24101148286554	21.901459579293448	31.653275251255973	22.204253686585034
37	21.08000611901484	22.0396230492605	33.251746468818524	23.628624362906145
38	20.986035533993313	21.189759877231197	29.331934701370223	28.492269887405268
39	25.329807615259657	19.574534214277215	31.83101806323468	23.264640107228452
40	21.88786176854098	19.18651097173355	32.778008454924475	26.147618804801
41	24.270635284861992	21.69069351263018	28.570700117281117	25.467971085226708
42	19.978826266114012	19.744264030633925	34.66251933438716	25.614390368864896
43	23.20150741444913	20.24689739237697	33.238391476115204	23.3132037170587
44	20.07862448431517	20.92266002316484	31.240727385748034	27.757988106771954
45	23.208306319825365	19.975183995376746	30.08054274690346	26.73596693789443
46	24.952711184927796	19.663405620266566	31.15404134220105	24.22984185260459
47	20.717478771632052	19.607557468961783	33.584650014204854	26.09031374520131
48	20.048757864269568	19.567006854753526	32.44704745393135	27.937187827045562
49	21.36458887262008	18.09771483933944	34.734879113034225	25.802817175006254
50	21.501781070390525	17.935512382506417	31.351452416160996	29.21125413094206
51	21.36313196432517	17.261692296111754	31.639434622454353	29.735741117108716
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	310.0
1	494.5
2	679.0
3	1542.5
4	2406.0
5	1686.0
6	966.0
7	995.5
8	1025.0
9	1105.0
10	1185.0
11	1183.0
12	1181.0
13	1229.0
14	1277.0
15	1253.5
16	1230.0
17	1217.0
18	1204.0
19	1221.5
20	1239.0
21	1327.5
22	1416.0
23	1503.5
24	1591.0
25	2324.5
26	3649.5
27	4241.0
28	4460.0
29	4679.0
30	5064.5
31	5450.0
32	6247.5
33	7045.0
34	7473.5
35	7902.0
36	8119.5
37	8337.0
38	9493.0
39	10649.0
40	12612.0
41	14575.0
42	17101.5
43	19628.0
44	23034.5
45	26441.0
46	35570.5
47	44700.0
48	48436.0
49	52172.0
50	50437.0
51	48702.0
52	40365.0
53	32028.0
54	26927.5
55	21827.0
56	19221.0
57	16615.0
58	15500.5
59	14386.0
60	13875.0
61	13364.0
62	12285.5
63	11207.0
64	9982.0
65	8757.0
66	7430.0
67	6103.0
68	5043.5
69	3984.0
70	3760.5
71	3537.0
72	3130.0
73	2723.0
74	2297.5
75	1415.5
76	959.0
77	791.0
78	623.0
79	465.0
80	307.0
81	215.0
82	123.0
83	100.5
84	78.0
85	52.0
86	26.0
87	21.5
88	17.0
89	12.0
90	7.0
91	3.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	411831.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.197551163493806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.50724081396775	27.6371117920228
2	11.191543280519795	8.7736218067326
3	4.4713913563697485	5.258027743899215
4	2.488481552297086	3.9016953186230205
5	1.802004669625863	3.531708511725726
6	1.5518668910461308	3.649762911642767
7	1.1858263010327923	3.2537040974024634
8	1.0374647675369184	3.253286264468046
9	0.9388823355898412	3.312169954720503
>10	4.702013349769725	27.791194070410175
>50	0.05947401756510187	1.640850063975986
>100	0.05451784943467672	4.24527761113337
>500	0.004336647114122012	1.1272496665645295
>1k	0.004956168130425156	2.6243401866787743
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	1815	0.4407147592094815	No Hit
GCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	1693	0.41109095721303157	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTC	1363	0.3309610009931258	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1356	0.32926127464906724	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCC	1259	0.30570792388139795	No Hit
CTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGCT	1258	0.30546510583224673	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCG	1059	0.25714431405115207	No Hit
TCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	1004	0.24378932134783443	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGC	876	0.21270861105647706	No Hit
ACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	802	0.19474007541928606	No Hit
TGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGCTT	772	0.18745553394474918	Illumina Single End Adapter 2 (95% over 21bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	556	0.1350068353280836	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG	552	0.13403556313147869	No Hit
ACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG	552	0.13403556313147869	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	532	0.1291792021484541	No Hit
GCCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG	473	0.11485293724853154	No Hit
AGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG	431	0.10465457918417992	No Hit
GGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG	422	0.10246921674181884	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.428180491512295E-4	0.0	0.0	0.3144493736508422	0.0
2	2.428180491512295E-4	0.0	0.0	1.6278522015098427	0.0
3	2.428180491512295E-4	0.0	0.0	2.482814552571322	0.0
4	2.428180491512295E-4	0.0	0.0	3.493423273138739	0.0
5	2.428180491512295E-4	0.0	0.0	6.525735070939293	0.0
6	2.428180491512295E-4	0.0	0.0	7.807085916310331	0.0
7	2.428180491512295E-4	0.0	0.0	10.376100876330339	0.0
8	2.428180491512295E-4	0.0	0.0	12.653005723221419	0.0
9	2.428180491512295E-4	0.0	0.0	14.86532096903827	0.0
10	2.428180491512295E-4	0.0	0.0	16.748132122156903	0.0
11	2.428180491512295E-4	0.0	0.0	19.827307803443645	0.0
12	2.428180491512295E-4	0.0	0.0	21.98620307844723	0.0
13	2.428180491512295E-4	0.0	0.0	22.744281027897365	0.0
14	2.428180491512295E-4	0.0	0.0	23.192280328581383	0.0
15	2.428180491512295E-4	0.0	0.0	24.242225573111302	0.0
16	2.428180491512295E-4	0.0	0.0	25.801603084760497	0.0
17	2.428180491512295E-4	0.0	0.0	28.627276722733352	0.0
18	2.428180491512295E-4	0.0	0.0	29.599277373485727	0.0
19	2.428180491512295E-4	0.0	0.0	30.877228766168646	0.0
20	2.428180491512295E-4	0.0	0.0	31.75841546653846	0.0
21	2.428180491512295E-4	0.0	0.0	32.75226974171444	0.0
22	2.428180491512295E-4	0.0	0.0	33.65846670114683	0.0
23	2.428180491512295E-4	0.0	0.0	34.37477994614296	0.0
24	2.428180491512295E-4	0.0	0.0	34.99955078660907	0.0
25	2.428180491512295E-4	0.0	0.0	35.60465336509393	0.0
26	2.428180491512295E-4	0.0	0.0	36.279687541734354	0.0
27	2.428180491512295E-4	0.0	0.0	36.85710886261597	0.0
28	4.85636098302459E-4	0.0	0.0	37.45249871913479	0.0
29	4.85636098302459E-4	0.0	0.0	38.08795355376356	0.0
30	4.85636098302459E-4	0.0	0.0	38.68844258931455	0.0
31	7.284541474536885E-4	0.0	0.0	39.253723007738614	0.0
32	7.284541474536885E-4	0.0	0.0	39.78913680611707	0.0
33	7.284541474536885E-4	0.0	0.0	40.3182373352176	0.0
34	7.284541474536885E-4	0.0	0.0	40.891045113165355	0.0
35	7.284541474536885E-4	0.0	0.0	41.42621609349466	0.0
36	7.284541474536885E-4	0.0	0.0	41.99271060216448	0.0
37	7.284541474536885E-4	0.0	0.0	42.5094274107583	0.0
38	7.284541474536885E-4	0.0	0.0	43.08684873163992	0.0
39	7.284541474536885E-4	0.0	0.0	43.60283708608628	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGG	20	7.028007E-4	45.000004	2
TGACGGT	20	7.028007E-4	45.000004	3
ATCGTGA	20	7.028007E-4	45.000004	29
GGTATCC	20	7.028007E-4	45.000004	7
TTCGAAG	20	7.028007E-4	45.000004	1
TCGCGGA	20	7.028007E-4	45.000004	3
AATCGTT	35	1.2093915E-7	45.000004	21
TCACGTC	20	7.028007E-4	45.000004	38
CCCGTAT	25	3.8864306E-5	45.0	5
ATAAGCG	25	3.8864306E-5	45.0	1
GTATACG	25	3.8864306E-5	45.0	1
GCAATCG	25	3.8864306E-5	45.0	45
CGGTATG	25	3.8864306E-5	45.0	6
CGGACCC	25	3.8864306E-5	45.0	6
CACGGTT	30	2.162098E-6	44.999996	4
TACGGAA	30	2.162098E-6	44.999996	4
GTATGCG	30	2.162098E-6	44.999996	1
TGATTAT	30	2.162098E-6	44.999996	27
CGAATAT	30	2.162098E-6	44.999996	14
ACGGTAA	30	2.162098E-6	44.999996	5
>>END_MODULE