FastQCFastQC Report
Sat 18 Jun 2016
SRR3551680_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551680_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419240
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27360.652609483827879No Hit
GAATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTC21990.5245205610151703No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCC17840.425531914893617No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCG17580.4193302165823871No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGC16200.38641351016124414No Hit
GCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC12530.29887415322965366No Hit
CCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC9000.2146741723118023No Hit
CTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGCT8420.2008396145405973No Hit
TCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC7740.18461978818814997No Hit
TGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGCTT7300.1741246064306841Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC6240.14884075946951628No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA6140.14645549088827403No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCG5860.13977673886079572No Hit
GAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCT5450.12999713767770252No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5240.12498807365709377No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGC5140.12260280507585153No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4840.1154469993321248No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCT4610.10996088159526762No Hit
AGCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTG4590.10948382787901918No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTC4230.10089686098654709No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGA253.8865513E-545.03
GAATGCG207.0281536E-445.01
TTTCGCA253.8865513E-545.013
CCGGATG253.8865513E-545.05
CCGTCAG253.8865513E-545.029
GTGCTAG253.8865513E-545.01
CGGATAT253.8865513E-545.06
GTATGCG207.0281536E-445.01
TGCGAAG406.7975634E-945.01
ACGGATA207.0281536E-445.05
ATCCATG207.0281536E-445.025
GAACGGT207.0281536E-445.03
GCTAGCG253.8865513E-545.01
TGCTCGG207.0281536E-445.02
AGTAGCG406.7975634E-945.01
TTCGCAA253.8865513E-545.014
CAATCGA207.0281536E-445.041
TCGACGG207.0281536E-445.02
GCGAACA302.1621909E-644.99999618
GCGATCT302.1621909E-644.9999968
Produced by FastQC (version 0.11.5)