##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551680_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 419240 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.40464888846484 34.0 31.0 34.0 30.0 34.0 2 32.55251168781605 34.0 31.0 34.0 31.0 34.0 3 32.57496422097128 34.0 31.0 34.0 31.0 34.0 4 36.05799780555291 37.0 35.0 37.0 35.0 37.0 5 36.06700935025284 37.0 35.0 37.0 35.0 37.0 6 36.095281938746304 37.0 35.0 37.0 35.0 37.0 7 36.208780173647554 37.0 36.0 37.0 35.0 37.0 8 35.87842763095124 37.0 35.0 37.0 35.0 37.0 9 37.56849775784753 39.0 37.0 39.0 35.0 39.0 10 37.67506678752027 39.0 37.0 39.0 35.0 39.0 11 37.73733183856502 39.0 37.0 39.0 35.0 39.0 12 37.71331218395191 39.0 37.0 39.0 35.0 39.0 13 37.68245396431638 39.0 37.0 39.0 35.0 39.0 14 39.05270966510829 40.0 38.0 41.0 35.0 41.0 15 39.0600968419044 40.0 39.0 41.0 35.0 41.0 16 39.070694590210856 40.0 39.0 41.0 35.0 41.0 17 39.0360247113825 40.0 38.0 41.0 35.0 41.0 18 38.92008396145406 40.0 38.0 41.0 35.0 41.0 19 38.87571081003721 40.0 38.0 41.0 35.0 41.0 20 38.70380450338708 40.0 38.0 41.0 35.0 41.0 21 38.703291670642116 40.0 38.0 41.0 35.0 41.0 22 38.67601850968419 40.0 38.0 41.0 35.0 41.0 23 38.67780030531438 40.0 38.0 41.0 35.0 41.0 24 38.61892472092357 40.0 38.0 41.0 35.0 41.0 25 38.61453821200267 40.0 38.0 41.0 35.0 41.0 26 38.55207756893426 40.0 38.0 41.0 35.0 41.0 27 38.44396527048946 40.0 38.0 41.0 35.0 41.0 28 38.38524711382502 40.0 38.0 41.0 35.0 41.0 29 38.311912031294725 40.0 38.0 41.0 34.0 41.0 30 38.18356788474382 40.0 38.0 41.0 34.0 41.0 31 38.057704417517414 40.0 38.0 41.0 34.0 41.0 32 37.86473141875775 40.0 37.0 41.0 33.0 41.0 33 37.671751264192345 40.0 37.0 41.0 33.0 41.0 34 37.55483493941418 40.0 37.0 41.0 33.0 41.0 35 37.43241102948192 40.0 37.0 41.0 33.0 41.0 36 37.373339853067456 40.0 37.0 41.0 33.0 41.0 37 37.304937505963174 40.0 37.0 41.0 32.0 41.0 38 37.24502432973953 40.0 37.0 41.0 32.0 41.0 39 37.207558916133955 40.0 37.0 41.0 32.0 41.0 40 37.12802452056101 40.0 37.0 41.0 32.0 41.0 41 37.093786375345864 40.0 36.0 41.0 32.0 41.0 42 37.02708711000859 40.0 36.0 41.0 32.0 41.0 43 36.99054241007538 40.0 36.0 41.0 31.0 41.0 44 36.94627659574468 40.0 36.0 41.0 31.0 41.0 45 36.883894666539454 40.0 36.0 41.0 31.0 41.0 46 36.78390659288236 40.0 35.0 41.0 31.0 41.0 47 36.67467321820437 40.0 35.0 41.0 31.0 41.0 48 36.68210571510352 40.0 35.0 41.0 31.0 41.0 49 36.62178942848965 40.0 35.0 41.0 31.0 41.0 50 36.52534586394428 39.0 35.0 41.0 31.0 41.0 51 35.86301879591642 39.0 35.0 41.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 3.0 11 2.0 12 2.0 13 0.0 14 4.0 15 5.0 16 13.0 17 28.0 18 83.0 19 189.0 20 367.0 21 824.0 22 1218.0 23 1732.0 24 2566.0 25 3400.0 26 4242.0 27 4368.0 28 4050.0 29 4148.0 30 4333.0 31 5164.0 32 6585.0 33 8984.0 34 15514.0 35 22063.0 36 25235.0 37 37608.0 38 73105.0 39 192864.0 40 540.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.11215532869001 23.554765766625323 24.5844862131476 14.748592691537066 2 34.2536494609293 27.858744394618835 21.222688674744777 16.66491746970709 3 29.98139490506631 27.59803453868906 25.626848583150462 16.79372197309417 4 28.174553954775305 28.163820246159716 25.64569220494228 18.0159335941227 5 28.751311897719685 30.82816525140731 21.076948764430874 19.343574086442132 6 37.790764240053434 28.464602614254364 18.862465413605573 14.882167732086632 7 76.15971758419998 5.506631046655854 12.802690582959642 5.530960786184524 8 67.15151226028051 9.111010399771015 15.490888274019653 8.246589065928823 9 30.96555672168686 27.903587443946186 21.311182139108865 19.819673695258086 10 24.675126419234804 28.211048564068314 29.880975097796014 17.232849918900868 11 23.889657475431733 20.866329548707185 34.2142925293388 21.02972044652228 12 23.329596412556054 21.416133956683524 35.04603568361798 20.20823394714245 13 20.61778456254174 27.19230035301975 33.20770918805457 18.982205896383935 14 17.643116114874534 25.65547180612537 37.7234996660624 18.977912412937698 15 18.932115256177845 25.529529625035778 34.26295200839615 21.27540311039023 16 18.291909168972424 24.71138250166969 31.31881499856884 25.677893330789047 17 21.0466558534491 24.344289667016504 30.832458734853546 23.77659574468085 18 20.66620551474096 27.63500620169831 32.004102661959735 19.694685621600993 19 21.94280125942181 23.95596794199027 31.159956111058108 22.941274687529816 20 19.96398244442324 29.376013739147027 31.498664249594505 19.161339566835224 21 18.84553000667875 27.816763667588972 29.95921190726076 23.378494418471522 22 17.453964316382027 28.176939223356552 30.45057723499666 23.918519225264763 23 17.837515504245776 25.335607289380786 31.47648125178895 25.35039595458449 24 19.206659669878828 28.199122221162103 30.46488884648411 22.129329262474958 25 17.569411315714152 28.417135769487643 29.90005724644595 24.113395668352254 26 17.657189199503865 28.863658047896195 29.209521992176317 24.269630760423624 27 21.533966224596888 26.08171930159336 31.476719778647077 20.907594695162675 28 19.116496517507873 27.98277836084343 27.523375632096176 25.377349489552525 29 19.535826734090257 27.23070317717775 28.09464745730369 25.138822631428297 30 20.992271729796773 28.573132334700883 25.085631142066596 25.34896479343574 31 23.016649174697072 28.23394714244824 24.065451769869288 24.683951912985403 32 19.445902108577425 30.227077568934263 23.581480774735237 26.74553954775308 33 20.206802785993702 27.95892567503101 25.816477435359218 26.017794103616065 34 19.80989409407499 26.318337944852587 25.092309894094072 28.779458066978343 35 20.357074706611964 29.836370575326782 25.160528575517603 24.64602614254365 36 20.676462169640303 28.824062589447575 25.086108195782845 25.41336704512928 37 20.384743822154373 27.99565881118214 26.930159335941227 24.68943803072226 38 19.915084438507776 27.269583055052 25.187482110485643 27.627850395954585 39 21.643211525617783 28.07580383551188 27.253124701841426 23.02785993702891 40 17.330168877015552 26.453821200267154 29.279648888464845 26.93636103425246 41 19.60356836179754 28.96240816715962 26.623413796393475 24.810609674649363 42 18.923528289285375 25.124511019940844 30.153372769773878 25.798587920999903 43 22.766911554241005 24.664392710619214 29.333317431542795 23.235378303596985 44 18.71004675126419 24.971853830741342 28.310514263906118 28.00758515408835 45 21.003482492128615 26.394666539452345 25.901392996851445 26.700457971567598 46 21.292099990458926 24.782463505390705 28.39161339566835 25.533823108482018 47 19.324491937792196 26.276595744680854 30.7236904875489 23.675221829978057 48 18.64158954298254 23.65113061730751 30.207995420284323 27.499284419425628 49 19.9005343001622 22.520274782940557 32.049422765003335 25.529768151893904 50 20.086585249499095 22.10929300639252 29.68657570842477 28.117546035683617 51 19.326638679515312 21.543984352638105 30.062971090544792 29.066405877301783 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 293.0 1 574.0 2 855.0 3 2873.5 4 4892.0 5 3310.5 6 1729.0 7 1802.0 8 1875.0 9 1909.0 10 1943.0 11 1966.0 12 1989.0 13 1984.5 14 1980.0 15 1906.0 16 1832.0 17 1775.0 18 1718.0 19 1687.5 20 1657.0 21 1802.5 22 1948.0 23 2151.0 24 2354.0 25 3044.5 26 4453.0 27 5171.0 28 5590.0 29 6009.0 30 6629.5 31 7250.0 32 7930.5 33 8611.0 34 9412.0 35 10213.0 36 11097.5 37 11982.0 38 12831.5 39 13681.0 40 15272.5 41 16864.0 42 18991.0 43 21118.0 44 24798.0 45 28478.0 46 38146.0 47 47814.0 48 48956.5 49 50099.0 50 47012.5 51 43926.0 52 36693.5 53 29461.0 54 24840.5 55 20220.0 56 17549.0 57 14878.0 58 13589.5 59 12301.0 60 11556.5 61 10812.0 62 9894.5 63 8977.0 64 7718.5 65 6460.0 66 5646.5 67 4833.0 68 4110.0 69 3387.0 70 3067.5 71 2748.0 72 2424.0 73 2100.0 74 1702.5 75 1001.0 76 697.0 77 592.0 78 487.0 79 384.5 80 282.0 81 233.0 82 184.0 83 118.5 84 53.0 85 41.5 86 30.0 87 19.5 88 9.0 89 4.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 419240.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.6680785179016 #Duplication Level Percentage of deduplicated Percentage of total 1 72.7779797382744 31.780745335852178 2 10.417467156378352 9.09821547484782 3 4.340515781383504 5.686259518489375 4 2.6648517741693545 4.65475826051987 5 1.925086633623601 4.203241713541913 6 1.472600682024057 3.858338532488518 7 1.206164126488398 3.686960884668009 8 1.0146318657290225 3.5445619183535957 9 0.7837817488791757 3.080361865585867 >10 3.2957983291561015 21.138366792475196 >50 0.056300448005866074 1.7243143633819094 >100 0.0360760152270593 3.2466949018162343 >500 0.005466062913190802 1.5880209661655202 >1k 0.0032796377479144814 2.709159471814017 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2736 0.652609483827879 No Hit GAATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTC 2199 0.5245205610151703 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCC 1784 0.425531914893617 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCG 1758 0.4193302165823871 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGC 1620 0.38641351016124414 No Hit GCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC 1253 0.29887415322965366 No Hit CCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC 900 0.2146741723118023 No Hit CTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGCT 842 0.2008396145405973 No Hit TCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC 774 0.18461978818814997 No Hit TGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGCTT 730 0.1741246064306841 Illumina Single End Adapter 1 (95% over 21bp) ACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC 624 0.14884075946951628 No Hit GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA 614 0.14645549088827403 No Hit CGTTTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCG 586 0.13977673886079572 No Hit GAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCT 545 0.12999713767770252 No Hit GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 524 0.12498807365709377 No Hit CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGC 514 0.12260280507585153 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 484 0.1154469993321248 No Hit GAATGACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCT 461 0.10996088159526762 No Hit AGCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTG 459 0.10948382787901918 No Hit GAATGATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTC 423 0.10089686098654709 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.7705371624844956E-4 0.0 0.0 0.20417899055433641 0.0 2 4.7705371624844956E-4 0.0 0.0 1.075040549565881 0.0 3 7.155805743726744E-4 0.0 0.0 1.710476099608816 0.0 4 7.155805743726744E-4 0.0 0.0 2.5865852494990937 0.0 5 7.155805743726744E-4 0.0 0.0 5.185573895620647 0.0 6 7.155805743726744E-4 0.0 0.0 6.322154374582578 0.0 7 7.155805743726744E-4 0.0 0.0 8.426915370670738 0.0 8 7.155805743726744E-4 0.0 0.0 10.490888274019655 0.0 9 7.155805743726744E-4 0.0 0.0 12.754746684476672 0.0 10 7.155805743726744E-4 0.0 0.0 14.579715675985115 0.0 11 7.155805743726744E-4 0.0 0.0 17.488312183951912 0.0 12 7.155805743726744E-4 0.0 0.0 19.724978532582767 0.0 13 7.155805743726744E-4 0.0 0.0 20.42624749546799 0.0 14 7.155805743726744E-4 0.0 0.0 20.863467226409693 0.0 15 7.155805743726744E-4 0.0 0.0 21.839757656712145 0.0 16 7.155805743726744E-4 0.0 0.0 23.350109722354738 0.0 17 7.155805743726744E-4 0.0 0.0 25.884934643640875 0.0 18 7.155805743726744E-4 0.0 0.0 26.77344719015361 0.0 19 7.155805743726744E-4 0.0 0.0 28.130187959164203 0.0 20 7.155805743726744E-4 0.0 0.0 28.98816906783704 0.0 21 7.155805743726744E-4 0.0 0.0 29.931304264860223 0.0 22 7.155805743726744E-4 0.0 0.0 30.803119931304266 0.0 23 7.155805743726744E-4 0.0 0.0 31.47528861749833 0.0 24 7.155805743726744E-4 0.0 0.0 32.09569697547944 0.0 25 7.155805743726744E-4 0.0 0.0 32.66959259612632 0.0 26 7.155805743726744E-4 0.0 0.0 33.334366949718536 0.0 27 7.155805743726744E-4 0.0 0.0 33.935454632191586 0.0 28 7.155805743726744E-4 0.0 0.0 34.54202843240149 0.0 29 7.155805743726744E-4 0.0 0.0 35.18557389562065 0.0 30 7.155805743726744E-4 0.0 0.0 35.80073466272302 0.0 31 7.155805743726744E-4 0.0 0.0 36.413510161244155 0.0 32 7.155805743726744E-4 0.0 0.0 36.97166300925484 0.0 33 7.155805743726744E-4 0.0 0.0 37.49260566739815 0.0 34 7.155805743726744E-4 0.0 0.0 38.094647457303694 0.0 35 7.155805743726744E-4 0.0 0.0 38.631094361225074 0.0 36 7.155805743726744E-4 0.0 0.0 39.20856788474382 0.0 37 7.155805743726744E-4 0.0 0.0 39.7528861749833 0.0 38 7.155805743726744E-4 0.0 0.0 40.31437839900773 0.0 39 7.155805743726744E-4 0.0 0.0 40.86561396813281 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAACGGA 25 3.8865513E-5 45.0 3 GAATGCG 20 7.0281536E-4 45.0 1 TTTCGCA 25 3.8865513E-5 45.0 13 CCGGATG 25 3.8865513E-5 45.0 5 CCGTCAG 25 3.8865513E-5 45.0 29 GTGCTAG 25 3.8865513E-5 45.0 1 CGGATAT 25 3.8865513E-5 45.0 6 GTATGCG 20 7.0281536E-4 45.0 1 TGCGAAG 40 6.7975634E-9 45.0 1 ACGGATA 20 7.0281536E-4 45.0 5 ATCCATG 20 7.0281536E-4 45.0 25 GAACGGT 20 7.0281536E-4 45.0 3 GCTAGCG 25 3.8865513E-5 45.0 1 TGCTCGG 20 7.0281536E-4 45.0 2 AGTAGCG 40 6.7975634E-9 45.0 1 TTCGCAA 25 3.8865513E-5 45.0 14 CAATCGA 20 7.0281536E-4 45.0 41 TCGACGG 20 7.0281536E-4 45.0 2 GCGAACA 30 2.1621909E-6 44.999996 18 GCGATCT 30 2.1621909E-6 44.999996 8 >>END_MODULE