FastQCFastQC Report
Sat 18 Jun 2016
SRR3551679_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551679_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391295
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC24650.6299594934767886TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT14820.3787423810679922TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13430.3432193102390779No Hit
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC12360.31587421255063314TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCC11840.3025850061973703No Hit
TCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC9720.24840593414176007TruSeq Adapter, Index 16 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC7650.19550467038934818No Hit
ACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC7460.19064899883719447TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG7240.18502664230312169No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6930.17710423082329188No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC6150.15717042129339756No Hit
TGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCTT6120.15640373631147855TruSeq Adapter, Index 16 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA6060.15487036634764054No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5370.13723661176350324No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC4680.11960285717936596No Hit
ACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG4060.10375803421970636No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG3940.1006912942920303No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCG302.161818E-645.0000041
CCCTCGT950.045.014
CGTTGAT207.027576E-445.024
GCTTGCG207.027576E-445.01
TCGGCGT453.8380676E-1045.04
AGCGGAT502.1827873E-1145.04
ACGGACC351.2091732E-745.05
CGAATAT1050.045.014
GCGATAT351.2091732E-745.08
CGTGTAC950.045.018
TATAGCG207.027576E-445.01
ACTCCGA207.027576E-445.035
AATCGTT207.027576E-445.021
TAATCGT207.027576E-445.020
CGGACCA207.027576E-445.06
GCTACGA1100.042.95454810
CGCGGAA650.041.5384644
TGACCCA603.6379788E-1241.25000410
TAGTGGT603.6379788E-1241.25000414
CTACGAA1150.041.0869611
Produced by FastQC (version 0.11.5)