##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551679_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	391295
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.454235295620954	34.0	31.0	34.0	31.0	34.0
2	32.581251996575475	34.0	31.0	34.0	31.0	34.0
3	32.632558555565495	34.0	31.0	34.0	31.0	34.0
4	36.083686221393066	37.0	35.0	37.0	35.0	37.0
5	36.103034794720095	37.0	35.0	37.0	35.0	37.0
6	36.138910540640694	37.0	36.0	37.0	35.0	37.0
7	36.24427861332243	37.0	37.0	37.0	35.0	37.0
8	35.90309357390204	37.0	36.0	37.0	35.0	37.0
9	37.65174101381311	39.0	37.0	39.0	35.0	39.0
10	37.732973843264034	39.0	37.0	39.0	35.0	39.0
11	37.76339845896319	39.0	37.0	39.0	35.0	39.0
12	37.719786861575024	39.0	37.0	39.0	35.0	39.0
13	37.679295672063276	39.0	37.0	39.0	35.0	39.0
14	39.0547745307249	40.0	38.0	41.0	35.0	41.0
15	39.07891999642214	40.0	38.0	41.0	35.0	41.0
16	39.09407480289807	40.0	39.0	41.0	35.0	41.0
17	39.05085677046729	40.0	38.0	41.0	35.0	41.0
18	38.98571665878685	40.0	38.0	41.0	35.0	41.0
19	38.994589759644256	40.0	38.0	41.0	35.0	41.0
20	38.880090468827866	40.0	38.0	41.0	35.0	41.0
21	38.85469275099349	40.0	38.0	41.0	35.0	41.0
22	38.84427094647261	40.0	38.0	41.0	35.0	41.0
23	38.852719814973355	40.0	38.0	41.0	35.0	41.0
24	38.792307594014744	40.0	38.0	41.0	35.0	41.0
25	38.75961870200233	40.0	38.0	41.0	35.0	41.0
26	38.72570822525205	40.0	38.0	41.0	35.0	41.0
27	38.66025888396223	40.0	38.0	41.0	35.0	41.0
28	38.58719124956874	40.0	38.0	41.0	35.0	41.0
29	38.55026770083952	40.0	38.0	41.0	35.0	41.0
30	38.41851033108013	40.0	38.0	41.0	35.0	41.0
31	38.36685109699843	40.0	38.0	41.0	35.0	41.0
32	38.25207835520515	40.0	38.0	41.0	34.0	41.0
33	38.154060235883414	40.0	38.0	41.0	34.0	41.0
34	38.06492799550212	40.0	38.0	41.0	34.0	41.0
35	38.0132662058038	40.0	38.0	41.0	34.0	41.0
36	37.94000434454823	40.0	37.0	41.0	34.0	41.0
37	37.860570669188206	40.0	37.0	41.0	34.0	41.0
38	37.79567589670198	40.0	37.0	41.0	34.0	41.0
39	37.72006286816852	40.0	37.0	41.0	33.0	41.0
40	37.65268148072426	40.0	37.0	41.0	33.0	41.0
41	37.65105099732938	40.0	37.0	41.0	33.0	41.0
42	37.598895973626036	40.0	37.0	41.0	33.0	41.0
43	37.55199274204884	40.0	36.0	41.0	33.0	41.0
44	37.43841858444396	40.0	36.0	41.0	33.0	41.0
45	37.36091439962177	40.0	36.0	41.0	33.0	41.0
46	37.27970712633691	40.0	36.0	41.0	33.0	41.0
47	37.21408911435107	40.0	35.0	41.0	33.0	41.0
48	37.20554568803588	40.0	35.0	41.0	33.0	41.0
49	37.14431566976322	39.0	35.0	41.0	33.0	41.0
50	37.02083338657535	39.0	35.0	41.0	32.0	41.0
51	36.33773495700175	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	0.0
13	0.0
14	5.0
15	5.0
16	10.0
17	32.0
18	68.0
19	108.0
20	247.0
21	438.0
22	712.0
23	1080.0
24	1562.0
25	2051.0
26	2510.0
27	2735.0
28	2753.0
29	2933.0
30	3555.0
31	4678.0
32	6013.0
33	8259.0
34	15521.0
35	22143.0
36	22908.0
37	36544.0
38	69044.0
39	184918.0
40	459.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.35804188655618	22.825745281692843	24.072375062293155	12.743837769457825
2	32.744604454439745	26.80790707778019	22.567372442786134	17.88011602499393
3	32.15757931995042	27.011845282970647	24.303913926832696	16.526661470246236
4	29.333622969882057	29.135307121225672	22.689530916571897	18.841538992320373
5	28.7412310405193	32.122056249121506	19.415019358795792	19.7216933515634
6	39.26986033555246	28.29987605259459	16.60741895500837	15.82284465684458
7	77.01989547528079	6.072400618459219	10.677877304846726	6.229826601413256
8	66.8459857652155	9.184119398407852	14.620682605195567	9.349212231181077
9	34.25982954037236	27.129148085204257	19.16916904126043	19.44185333316296
10	26.359907486678846	27.18792726715138	27.75450746878953	18.697657777380236
11	24.894772486231613	21.185806105368073	32.247025901174304	21.672395507226007
12	24.375982315133086	21.330709566950766	33.66181525447553	20.631492863440627
13	20.17735979248393	27.831687090302715	31.468585082865868	20.522368034347487
14	17.598231513308374	24.570720300540515	36.58850739212103	21.24254079403008
15	19.606179480954268	24.9236509538839	33.19464853882621	22.27552102633563
16	19.21593682515749	24.026118401717376	29.410035906413317	27.34790886671182
17	21.00103502472559	23.915460202660395	29.780344752680204	25.303160019933806
18	21.514713962611328	26.02537727290152	31.68427912444575	20.7756296400414
19	24.525741448267933	22.721731685812493	29.437125442441126	23.31540142347845
20	20.779207503290355	27.78619711470885	30.546263049617295	20.8883323323835
21	20.0618459218748	27.07215783488161	28.257452816928403	24.608543426315187
22	18.141811165488953	27.6847391354349	28.924468751197946	25.248980947878195
23	18.903129352534535	23.716122107361453	30.546007487956654	26.834741052147358
24	20.941489157796546	26.12402407390843	29.694220473044634	23.240266295250386
25	18.412450964106363	26.74018323771068	28.86032277437739	25.98704302380557
26	19.297716556562182	27.502523671398816	28.111782670363795	25.087977101675207
27	21.831354860143882	24.770058395839456	30.68963314123615	22.70895360278051
28	20.00715572649791	26.00416565506843	27.885866162358326	26.10281245607534
29	23.574796509027713	23.527262040148734	27.65688291442518	25.24105853639837
30	22.44700290062485	26.17615865267893	26.032021876078147	25.34481657061808
31	24.312347461633806	24.646110990429214	25.96813146091823	25.073410087018743
32	22.99109367612671	26.137824403582975	25.6522572483676	25.218824671922718
33	21.271930385003643	21.652717259356752	31.39549444792292	25.679857907716684
34	21.44443450593542	21.824199133645973	28.83578885495598	27.895577505462633
35	23.744745013353096	22.00181448779054	30.344624899372597	23.908815599483766
36	24.222389757088642	23.06495099605157	28.860578336038028	23.85208091082176
37	20.601592149145787	23.295723175609197	33.04616721399455	23.056517461250465
38	21.503213687882543	23.253044378282368	29.958215668485416	25.285526265349674
39	25.14854521524681	20.44978852272582	32.62653496722422	21.775131294803156
40	19.747505079288004	20.067468278408874	32.256481682617974	27.928544959685148
41	22.25814283341213	21.982647363242567	29.86825796394025	25.89095183940505
42	19.96166575090405	20.506778773048467	33.00476622497093	26.526789251076554
43	23.912904586054	20.331974597170934	33.07913466821707	22.675986148557996
44	21.890645165412284	21.41248929835546	30.300923855403212	26.395941680829043
45	22.346056044672178	20.53463499405819	30.505117622254307	26.61419133901532
46	22.209586117890595	20.568880256583906	31.792381706896332	25.429151918629167
47	20.041656550684266	20.291851416450505	35.728286842407904	23.938205190457328
48	19.455909224498143	20.14950357147421	33.53914565736848	26.85544154665917
49	21.23487394421089	18.735480903154908	35.212307849576405	24.817337303057798
50	20.897532552166524	19.06029977382793	31.410317024240026	28.63185064976552
51	21.185039420386154	18.116510561085626	31.496185742214955	29.202264276313265
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	353.0
1	551.5
2	750.0
3	1544.0
4	2338.0
5	1620.5
6	903.0
7	933.0
8	963.0
9	999.0
10	1035.0
11	1057.0
12	1079.0
13	1158.5
14	1238.0
15	1168.0
16	1098.0
17	1219.0
18	1340.0
19	1362.0
20	1384.0
21	1420.0
22	1456.0
23	1633.5
24	1811.0
25	2497.5
26	3596.5
27	4009.0
28	4492.5
29	4976.0
30	5390.0
31	5804.0
32	6214.5
33	6625.0
34	7523.5
35	8422.0
36	8818.5
37	9215.0
38	10040.5
39	10866.0
40	12365.5
41	13865.0
42	16486.0
43	19107.0
44	22748.5
45	26390.0
46	32966.5
47	39543.0
48	44077.5
49	48612.0
50	46085.0
51	43558.0
52	36613.5
53	29669.0
54	25370.5
55	21072.0
56	18761.0
57	16450.0
58	15997.0
59	15544.0
60	14303.0
61	13062.0
62	11812.5
63	10563.0
64	9293.5
65	8024.0
66	6759.0
67	5494.0
68	4621.5
69	3749.0
70	3338.5
71	2928.0
72	2469.0
73	2010.0
74	1718.0
75	1065.0
76	704.0
77	524.0
78	344.0
79	270.0
80	196.0
81	145.0
82	94.0
83	59.0
84	24.0
85	14.0
86	4.0
87	8.5
88	13.0
89	7.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	391295.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.56398541354282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.96844454686995	27.368260603273146
2	11.057719922670536	8.528594996098168
3	4.3334906817847765	5.013500143262155
4	2.334210830031179	3.600658896058242
5	1.6666085883837798	3.213553464625863
6	1.3296273178389473	3.076543709435353
7	1.1242045918491772	3.0347666637336665
8	0.9563255235882031	2.9503778833803325
9	0.8654053652686509	3.0036131894719755
>10	5.243342161819664	31.388691850322093
>50	0.06031023494755928	1.5380969867257788
>100	0.05103173726331939	3.710294775057848
>500	0.005964748511297071	1.6025038396096098
>1k	0.0033137491729428176	1.9705429989457883
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	2465	0.6299594934767886	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT	1482	0.3787423810679922	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1343	0.3432193102390779	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	1236	0.31587421255063314	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCC	1184	0.3025850061973703	No Hit
TCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	972	0.24840593414176007	TruSeq Adapter, Index 16 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC	765	0.19550467038934818	No Hit
ACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	746	0.19064899883719447	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG	724	0.18502664230312169	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	693	0.17710423082329188	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	615	0.15717042129339756	No Hit
TGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCTT	612	0.15640373631147855	TruSeq Adapter, Index 16 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	606	0.15487036634764054	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	537	0.13723661176350324	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	468	0.11960285717936596	No Hit
ACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	406	0.10375803421970636	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG	394	0.1006912942920303	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5556166063967086E-4	0.0	0.0	0.38538698424462364	0.0
2	2.5556166063967086E-4	0.0	0.0	1.8093765573288696	0.0
3	2.5556166063967086E-4	0.0	0.0	2.5282715087082637	0.0
4	2.5556166063967086E-4	0.0	0.0	3.38491419517244	0.0
5	2.5556166063967086E-4	0.0	0.0	5.785660435221508	0.0
6	2.5556166063967086E-4	0.0	0.0	6.825796394024969	0.0
7	2.5556166063967086E-4	0.0	0.0	8.821988525281437	0.0
8	2.5556166063967086E-4	0.0	0.0	10.786491010618587	0.0
9	2.5556166063967086E-4	0.0	0.0	12.59203414303786	0.0
10	2.5556166063967086E-4	0.0	0.0	14.205906029977383	0.0
11	2.5556166063967086E-4	0.0	0.0	17.097841781775898	0.0
12	2.5556166063967086E-4	0.0	0.0	19.025287826320294	0.0
13	2.5556166063967086E-4	0.0	0.0	19.73012688636451	0.0
14	2.5556166063967086E-4	0.0	0.0	20.16918181934346	0.0
15	2.5556166063967086E-4	0.0	0.0	21.18836172197447	0.0
16	2.5556166063967086E-4	0.0	0.0	22.759810373247806	0.0
17	5.111233212793417E-4	0.0	0.0	25.663501961435745	0.0
18	5.111233212793417E-4	0.0	0.0	26.602946625947176	0.0
19	5.111233212793417E-4	0.0	0.0	27.87998824416361	0.0
20	5.111233212793417E-4	0.0	0.0	28.76576495994071	0.0
21	5.111233212793417E-4	0.0	0.0	29.754021901634317	0.0
22	5.111233212793417E-4	0.0	0.0	30.701133415964936	0.0
23	5.111233212793417E-4	0.0	0.0	31.486985522431926	0.0
24	5.111233212793417E-4	0.0	0.0	32.15706819662914	0.0
25	5.111233212793417E-4	0.0	0.0	32.74971568765254	0.0
26	7.666849819190125E-4	0.0	0.0	33.420053923510395	0.0
27	7.666849819190125E-4	0.0	0.0	33.99532322161029	0.0
28	7.666849819190125E-4	0.0	0.0	34.65313893609681	0.0
29	7.666849819190125E-4	0.0	0.0	35.29434314264174	0.0
30	7.666849819190125E-4	0.0	0.0	35.91868027958446	0.0
31	7.666849819190125E-4	0.0	0.0	36.502638674146105	0.0
32	7.666849819190125E-4	0.0	0.0	37.07995246553112	0.0
33	7.666849819190125E-4	0.0	0.0	37.62327655605106	0.0
34	7.666849819190125E-4	0.0	0.0	38.22691319848197	0.0
35	7.666849819190125E-4	0.0	0.0	38.760014822576316	0.0
36	7.666849819190125E-4	0.0	0.0	39.31918373605592	0.0
37	0.0010222466425586834	0.0	0.0	39.84589631863428	0.0
38	0.0010222466425586834	0.0	0.0	40.393053834063814	0.0
39	0.0010222466425586834	0.0	0.0	40.92973332140712	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTAGCG	30	2.161818E-6	45.000004	1
CCCTCGT	95	0.0	45.0	14
CGTTGAT	20	7.027576E-4	45.0	24
GCTTGCG	20	7.027576E-4	45.0	1
TCGGCGT	45	3.8380676E-10	45.0	4
AGCGGAT	50	2.1827873E-11	45.0	4
ACGGACC	35	1.2091732E-7	45.0	5
CGAATAT	105	0.0	45.0	14
GCGATAT	35	1.2091732E-7	45.0	8
CGTGTAC	95	0.0	45.0	18
TATAGCG	20	7.027576E-4	45.0	1
ACTCCGA	20	7.027576E-4	45.0	35
AATCGTT	20	7.027576E-4	45.0	21
TAATCGT	20	7.027576E-4	45.0	20
CGGACCA	20	7.027576E-4	45.0	6
GCTACGA	110	0.0	42.954548	10
CGCGGAA	65	0.0	41.538464	4
TGACCCA	60	3.6379788E-12	41.250004	10
TAGTGGT	60	3.6379788E-12	41.250004	14
CTACGAA	115	0.0	41.08696	11
>>END_MODULE