FastQCFastQC Report
Sat 18 Jun 2016
SRR3551678_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551678_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635799
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC34380.5407369310112158Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27610.4342567383717181No Hit
CTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCT21890.34429119894809523Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC18310.28798409560254107Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCC16550.2603023911645032No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTC15200.23906926560123562No Hit
TCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC14620.2299468857296095Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCTT11800.1855932456641171Illumina Single End Adapter 1 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC10790.16970772209456134Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCG10700.1682921803903435No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGC8480.1333754850196367No Hit
AGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG6690.10522193334685961No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG207.030906E-445.01
TAGAACG207.030906E-445.01
CACGCGT207.030906E-445.038
ATAGACG207.030906E-445.01
ATACTCG207.030906E-445.043
TGTTGCG207.030906E-445.01
TCGCTTA207.030906E-445.028
CACACGG253.888832E-545.02
TAAGACG253.888832E-545.01
ACGGTCA207.030906E-445.05
TATCGCG556.002665E-1140.90909232
TACACGG556.002665E-1140.9090922
TCGACAC501.0804797E-940.534
CGTTTTT16100.040.2484471
CGGAATC1200.039.3750046
ACAACGG403.4549703E-739.3752
GTATGCG403.4549703E-739.3751
TGTAGCG403.4549703E-739.3751
GCGATTC356.243883E-638.571438
GCACGGA1000.038.253