##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551678_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	635799
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43281445865753	34.0	31.0	34.0	30.0	34.0
2	32.568649840594276	34.0	31.0	34.0	31.0	34.0
3	32.6132456955736	34.0	31.0	34.0	31.0	34.0
4	36.06875443339798	37.0	35.0	37.0	35.0	37.0
5	36.08424989658681	37.0	35.0	37.0	35.0	37.0
6	36.12058999778232	37.0	36.0	37.0	35.0	37.0
7	36.23875784642631	37.0	37.0	37.0	35.0	37.0
8	35.93636510909894	37.0	36.0	37.0	35.0	37.0
9	37.59458099179143	39.0	37.0	39.0	35.0	39.0
10	37.70829932101183	39.0	37.0	39.0	35.0	39.0
11	37.77566652353967	39.0	37.0	39.0	35.0	39.0
12	37.76627990921659	39.0	38.0	39.0	35.0	39.0
13	37.73334811788002	39.0	38.0	39.0	35.0	39.0
14	39.122562319223526	40.0	39.0	41.0	35.0	41.0
15	39.133955857118366	40.0	39.0	41.0	35.0	41.0
16	39.148364498843186	40.0	39.0	41.0	35.0	41.0
17	39.126701992296304	40.0	39.0	41.0	35.0	41.0
18	39.051124647884	40.0	38.0	41.0	35.0	41.0
19	39.05064179088045	40.0	39.0	41.0	35.0	41.0
20	38.943189592937394	40.0	38.0	41.0	35.0	41.0
21	38.91894922766472	40.0	38.0	41.0	35.0	41.0
22	38.90536474577657	40.0	38.0	41.0	35.0	41.0
23	38.92557396284046	40.0	38.0	41.0	35.0	41.0
24	38.86564149990799	40.0	38.0	41.0	35.0	41.0
25	38.848282240141934	40.0	38.0	41.0	35.0	41.0
26	38.80039131864001	40.0	38.0	41.0	35.0	41.0
27	38.72608481611327	40.0	38.0	41.0	35.0	41.0
28	38.67842509975637	40.0	38.0	41.0	35.0	41.0
29	38.65121524255307	40.0	38.0	41.0	35.0	41.0
30	38.55607668461259	40.0	38.0	41.0	35.0	41.0
31	38.47719798238123	40.0	38.0	41.0	35.0	41.0
32	38.386941470496176	40.0	38.0	41.0	35.0	41.0
33	38.279895061175	40.0	38.0	41.0	34.0	41.0
34	38.194591372430594	40.0	38.0	41.0	34.0	41.0
35	38.14176021038095	40.0	38.0	41.0	34.0	41.0
36	38.07041533566426	40.0	38.0	41.0	34.0	41.0
37	38.01143757697008	40.0	38.0	41.0	34.0	41.0
38	37.953880078452464	40.0	38.0	41.0	34.0	41.0
39	37.89290955160357	40.0	38.0	41.0	34.0	41.0
40	37.82460966437506	40.0	37.0	41.0	33.0	41.0
41	37.797017610911624	40.0	37.0	41.0	33.0	41.0
42	37.75063345491264	40.0	37.0	41.0	33.0	41.0
43	37.725745086104254	40.0	37.0	41.0	33.0	41.0
44	37.68740435263346	40.0	37.0	41.0	33.0	41.0
45	37.650056071179726	40.0	37.0	41.0	33.0	41.0
46	37.55038620696163	40.0	37.0	41.0	33.0	41.0
47	37.46185036465927	40.0	36.0	41.0	33.0	41.0
48	37.42857255201722	40.0	36.0	41.0	33.0	41.0
49	37.40605757479958	40.0	36.0	41.0	33.0	41.0
50	37.30861482952946	40.0	36.0	41.0	33.0	41.0
51	36.6353973504205	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	2.0
13	5.0
14	7.0
15	13.0
16	19.0
17	31.0
18	82.0
19	199.0
20	345.0
21	670.0
22	1054.0
23	1519.0
24	2407.0
25	3276.0
26	4084.0
27	4567.0
28	4651.0
29	4792.0
30	5626.0
31	7158.0
32	9348.0
33	12704.0
34	22504.0
35	31181.0
36	36460.0
37	56702.0
38	109619.0
39	315908.0
40	864.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.985121083864556	22.83174399456432	24.154174511126943	13.028960410444181
2	33.599927020961026	26.091264692143273	22.480060522271973	17.828747764623724
3	31.741478045734578	26.275756960926334	24.778428402687013	17.204336590652076
4	28.661259297356555	28.28346694474197	23.583239357092413	19.47203440080906
5	27.901742531837893	31.061860745298436	20.310035089706023	20.72636163315765
6	37.89609609326218	27.73219209215491	17.97675051392028	16.39496130066263
7	77.10723042974273	5.405481921173201	10.944811174600778	6.542476474483289
8	67.62357285871794	8.178056272501214	14.868220931457898	9.33014993732296
9	31.53716819309247	26.640180308556634	21.50475228806588	20.317899210285013
10	24.552256294835317	27.725743513280136	28.77182883269713	18.950171359187415
11	23.283616363032973	21.512459126233292	32.58230981803998	22.621614692693758
12	22.804691419772603	21.163292172526223	34.809900613244125	21.222115794457054
13	20.166436247933703	26.855342647597745	31.830657173100303	21.147563931368246
14	17.941833818549576	24.745241813843684	37.116919026296046	20.196005341310695
15	19.093770200959735	24.679969613038082	33.54739469549339	22.67886549050879
16	18.696474829309263	24.981479996036484	30.05273679260269	26.26930838205156
17	20.172727544396892	24.5632660636459	30.1148633451767	25.14914304678051
18	20.726676197980808	27.410077713239563	31.2051450222476	20.658101066532033
19	22.698840356779424	23.482735896092947	29.839933689735275	23.978490057392353
20	20.06955028240057	28.6305892270985	30.3223188460504	20.977541644450525
21	19.255299237652153	26.462923030706246	29.13499392103479	25.14678381060681
22	17.80515540288676	27.94232139402547	29.149306620488552	25.10321658259922
23	17.87231499263132	24.118785968521497	30.826251692751956	27.18264734609523
24	20.33236919215035	26.766006237820445	28.837258315914305	24.0643662541149
25	18.10650850347358	26.82561627180917	29.225116742869993	25.842758481847248
26	18.283608498912393	27.177928873747838	27.998785779782605	26.53967684755717
27	20.83299910820873	25.052571646070536	30.886176291563842	23.228252954156893
28	18.694430157958724	25.67934205621588	28.14961961248759	27.4766081733378
29	19.18357845797178	23.39497231043144	28.305329199951558	29.11612003164522
30	20.451746542539386	25.404412400774458	26.870441759109404	27.273399297576752
31	20.93381713403135	25.6683322874053	26.369025430993126	27.028825147570224
32	19.08543423314601	26.631844340742905	25.222436650576675	29.060284775534406
33	18.87499036645229	23.364616804996547	30.21174931070983	27.54864351784133
34	18.65856976811854	24.693181335610785	27.60746713977216	29.040781756498518
35	20.48257389520902	23.917149916876244	28.568148109701337	27.032128078213397
36	20.73170923515136	27.571764032343555	26.108565757417047	25.587960975088038
37	19.76033306123476	25.98808743014695	30.242576663379467	24.009002845238825
38	19.872789985514288	26.8339522396229	26.580098427333166	26.71315934752964
39	23.219287856696848	24.211739873765136	29.349841695252742	23.21913057428527
40	18.73154880709155	25.230929900801986	29.783626586389722	26.253894705716746
41	21.74272057678606	25.612025184059746	26.274498701633693	26.37075553752051
42	19.757187413003166	24.782045898153346	28.816968884820515	26.643797804022967
43	21.72054375675331	23.497205877958287	30.11533519241144	24.666915172876962
44	18.17288168116024	23.18688767991142	29.911654469415648	28.728576169512692
45	21.097390842074304	22.941684400258573	28.4868331029146	27.474091654752524
46	21.9390090264376	23.647567863428538	29.130747295922138	25.282675814211725
47	21.082920860208965	23.385063518501916	30.637040951621504	24.894974669667615
48	18.661558133938556	24.430047861037842	29.225116742869993	27.683277262153606
49	20.603681352125435	20.6793341920953	32.73330093315655	25.983683522622712
50	20.37593642015794	21.479429819801542	29.379096223806577	28.765537536233936
51	20.145674969605174	20.386317059322206	30.318229503349333	29.14977846772329
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	291.0
1	577.0
2	863.0
3	2802.0
4	4741.0
5	3188.0
6	1635.0
7	1657.5
8	1680.0
9	1667.0
10	1654.0
11	1688.0
12	1722.0
13	1730.0
14	1738.0
15	1653.5
16	1569.0
17	1626.0
18	1683.0
19	1721.5
20	1760.0
21	1887.0
22	2014.0
23	2326.0
24	2638.0
25	3485.5
26	5398.0
27	6463.0
28	7080.0
29	7697.0
30	8756.0
31	9815.0
32	10715.0
33	11615.0
34	13234.0
35	14853.0
36	15758.0
37	16663.0
38	18095.0
39	19527.0
40	21978.0
41	24429.0
42	28639.5
43	32850.0
44	38519.5
45	44189.0
46	56539.0
47	68889.0
48	74445.5
49	80002.0
50	76447.0
51	72892.0
52	60302.5
53	47713.0
54	41006.5
55	34300.0
56	29963.5
57	25627.0
58	23381.5
59	21136.0
60	20542.5
61	19949.0
62	17609.5
63	15270.0
64	13050.0
65	10830.0
66	9032.5
67	7235.0
68	6381.5
69	5528.0
70	4683.0
71	3838.0
72	3204.5
73	2571.0
74	2143.0
75	1336.0
76	957.0
77	739.5
78	522.0
79	392.0
80	262.0
81	175.5
82	89.0
83	66.0
84	43.0
85	25.5
86	8.0
87	4.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	635799.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.310638260276065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.97280866690183	27.190135991534614
2	11.24988583574769	8.619806134454665
3	4.276865565608314	4.915483487155533
4	2.3803514528992733	3.6477113377738646
5	1.6149886146653654	3.093562230545459
6	1.3381077069302292	3.0758256188094903
7	1.1013166648914456	2.9534501051068895
8	0.9464248542439074	2.900651218517827
9	0.8339942205887861	2.8755765806524076
>10	5.163239900024668	31.453182209806773
>50	0.06737612424194434	1.7531850876407855
>100	0.046834622791307436	3.7975446344644266
>500	0.003697470220366376	0.8617190348844801
>1k	0.004108300244851529	2.862166328652835
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	3438	0.5407369310112158	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2761	0.4342567383717181	No Hit
CTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCT	2189	0.34429119894809523	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	1831	0.28798409560254107	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCC	1655	0.2603023911645032	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTC	1520	0.23906926560123562	No Hit
TCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	1462	0.2299468857296095	Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCTT	1180	0.1855932456641171	Illumina Single End Adapter 1 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	1079	0.16970772209456134	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCG	1070	0.1682921803903435	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGC	848	0.1333754850196367	No Hit
AGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	669	0.10522193334685961	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.351683472292344	0.0
2	0.0	0.0	0.0	1.6058534222293523	0.0
3	0.0	0.0	0.0	2.2934921256560643	0.0
4	0.0	0.0	0.0	3.165623097865835	0.0
5	0.0	0.0	0.0	5.690163086132567	0.0
6	0.0	0.0	0.0	6.8930589698945735	0.0
7	0.0	0.0	0.0	9.194887063364366	0.0
8	0.0	0.0	0.0	11.200395093417889	0.0
9	0.0	0.0	0.0	13.303103653827703	0.0
10	0.0	0.0	0.0	14.78800690155222	0.0
11	0.0	0.0	0.0	17.549571484069652	0.0
12	0.0	0.0	0.0	19.455362465181604	0.0
13	0.0	0.0	0.0	20.162976034878948	0.0
14	0.0	0.0	0.0	20.576314212510557	0.0
15	0.0	0.0	0.0	21.506954241827998	0.0
16	0.0	0.0	0.0	22.969680669519768	0.0
17	0.0	0.0	0.0	25.490603162320166	0.0
18	0.0	0.0	0.0	26.376417704337378	0.0
19	0.0	0.0	0.0	27.584975754916254	0.0
20	0.0	0.0	0.0	28.450972713074414	0.0
21	0.0	0.0	0.0	29.49060945361663	0.0
22	1.5728241157976028E-4	0.0	0.0	30.517820883644045	0.0
23	1.5728241157976028E-4	0.0	0.0	31.292122195851203	0.0
24	1.5728241157976028E-4	0.0	0.0	31.993287186673776	0.0
25	1.5728241157976028E-4	0.0	0.0	32.65560342183615	0.0
26	1.5728241157976028E-4	0.0	0.0	33.36919372317352	0.0
27	1.5728241157976028E-4	0.0	0.0	34.01499530512002	0.0
28	1.5728241157976028E-4	0.0	0.0	34.68769217944665	0.0
29	1.5728241157976028E-4	0.0	0.0	35.36384926682804	0.0
30	1.5728241157976028E-4	0.0	0.0	36.04582580343788	0.0
31	1.5728241157976028E-4	0.0	0.0	36.69162738538437	0.0
32	1.5728241157976028E-4	0.0	0.0	37.28615490115587	0.0
33	1.5728241157976028E-4	0.0	0.0	37.88178339380842	0.0
34	1.5728241157976028E-4	0.0	0.0	38.52491117475806	0.0
35	1.5728241157976028E-4	0.0	0.0	39.113304676477945	0.0
36	1.5728241157976028E-4	0.0	0.0	39.69509231691148	0.0
37	1.5728241157976028E-4	0.0	0.0	40.25265846596173	0.0
38	1.5728241157976028E-4	0.0	0.0	40.8854056077471	0.0
39	1.5728241157976028E-4	0.0	0.0	41.462317493421665	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.030906E-4	45.0	1
TAGAACG	20	7.030906E-4	45.0	1
CACGCGT	20	7.030906E-4	45.0	38
ATAGACG	20	7.030906E-4	45.0	1
ATACTCG	20	7.030906E-4	45.0	43
TGTTGCG	20	7.030906E-4	45.0	1
TCGCTTA	20	7.030906E-4	45.0	28
CACACGG	25	3.888832E-5	45.0	2
TAAGACG	25	3.888832E-5	45.0	1
ACGGTCA	20	7.030906E-4	45.0	5
TATCGCG	55	6.002665E-11	40.909092	32
TACACGG	55	6.002665E-11	40.909092	2
TCGACAC	50	1.0804797E-9	40.5	34
CGTTTTT	1610	0.0	40.248447	1
CGGAATC	120	0.0	39.375004	6
ACAACGG	40	3.4549703E-7	39.375	2
GTATGCG	40	3.4549703E-7	39.375	1
TGTAGCG	40	3.4549703E-7	39.375	1
GCGATTC	35	6.243883E-6	38.57143	8
GCACGGA	100	0.0	38.25	3
>>END_MODULE