SRR3551676_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551676_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	404379
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGC	1855	0.4587280744054464	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1800	0.44512697246889676	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCG	1703	0.4211395745080729	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTC	1653	0.4087749363839368	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	1504	0.3719283147740115	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCC	1160	0.2868596044799557	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCT	1139	0.28166645646781857	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	954	0.2359172954085153	No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	862	0.213166361260105	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	794	0.19635045341128	No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	580	0.14342980223997784	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTAGCCAGT	570	0.14095687461515063	No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	486	0.12018428256660213	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	473	0.11696947665432676	No Hit
GAACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCT	442	0.10930340101736243	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	441	0.10905610825487971	No Hit
TGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCTT	427	0.10559400958012162	Illumina Single End Adapter 2 (95% over 21bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	424	0.10485213129267346	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCT	421	0.10411025300522529	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	407	0.10064815433046721	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	20	7.0278556E-4	45.000004	1
GGCGTAA	20	7.0278556E-4	45.000004	7
TAACGGC	20	7.0278556E-4	45.000004	3
GCGCAAA	20	7.0278556E-4	45.000004	10
CATACGA	65	0.0	45.000004	17
TTAGTCA	40	6.7957444E-9	45.000004	32
CGACAGG	35	1.2093187E-7	45.0	2
TAACGGA	35	1.2093187E-7	45.0	3
CCTAACG	25	3.886305E-5	45.0	1
GCCGATT	25	3.886305E-5	45.0	8
TACGAAT	50	2.1827873E-11	45.0	12
TTAGTAG	25	3.886305E-5	45.0	1
TATCGCG	25	3.886305E-5	45.0	32
AAGCACG	30	2.1619999E-6	44.999996	1
CGAATAT	55	6.002665E-11	40.909092	14
GGCCGAT	105	0.0	40.714287	7
ACGGAGT	40	3.4508048E-7	39.375004	5
GTCGTTT	40	3.4508048E-7	39.375004	8
TTTAGCG	35	6.2382496E-6	38.571426	1
CGATTAG	35	6.2382496E-6	38.571426	9

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content