##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551676_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	404379
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52910017582516	34.0	31.0	34.0	31.0	34.0
2	32.66218819473811	34.0	31.0	34.0	31.0	34.0
3	32.69259283988536	34.0	31.0	34.0	31.0	34.0
4	36.135093563216685	37.0	37.0	37.0	35.0	37.0
5	36.14983468478828	37.0	35.0	37.0	35.0	37.0
6	36.17916607934636	37.0	37.0	37.0	35.0	37.0
7	36.258453084853564	37.0	37.0	37.0	35.0	37.0
8	35.918242539795585	37.0	37.0	37.0	35.0	37.0
9	37.70577107119806	39.0	37.0	39.0	35.0	39.0
10	37.777293083963315	39.0	37.0	39.0	35.0	39.0
11	37.81506705343255	39.0	38.0	39.0	35.0	39.0
12	37.796831685127074	39.0	38.0	39.0	35.0	39.0
13	37.745273617077046	39.0	38.0	39.0	35.0	39.0
14	39.16142282363822	40.0	39.0	41.0	35.0	41.0
15	39.1677955581274	40.0	39.0	41.0	35.0	41.0
16	39.18446803617398	41.0	39.0	41.0	35.0	41.0
17	39.144438756710905	40.0	39.0	41.0	35.0	41.0
18	39.06787939037388	40.0	39.0	41.0	35.0	41.0
19	39.037603337463125	40.0	39.0	41.0	35.0	41.0
20	38.91799277410548	40.0	38.0	41.0	35.0	41.0
21	38.917569903481635	40.0	38.0	41.0	35.0	41.0
22	38.89811043600187	40.0	38.0	41.0	35.0	41.0
23	38.886361556856315	40.0	38.0	41.0	35.0	41.0
24	38.82325491679835	40.0	38.0	41.0	35.0	41.0
25	38.80013551643384	40.0	38.0	41.0	35.0	41.0
26	38.73360139868787	40.0	38.0	41.0	35.0	41.0
27	38.63035914327895	40.0	38.0	41.0	35.0	41.0
28	38.58248573738992	40.0	38.0	41.0	35.0	41.0
29	38.558656606797086	40.0	38.0	41.0	35.0	41.0
30	38.44284693319881	40.0	38.0	41.0	35.0	41.0
31	38.36007804559584	40.0	38.0	41.0	35.0	41.0
32	38.204026420758744	40.0	38.0	41.0	34.0	41.0
33	38.08213581813101	40.0	38.0	41.0	34.0	41.0
34	37.973997166024944	40.0	38.0	41.0	34.0	41.0
35	37.89072380118651	40.0	38.0	41.0	34.0	41.0
36	37.8428825433566	40.0	37.0	41.0	34.0	41.0
37	37.7283662109061	40.0	37.0	41.0	34.0	41.0
38	37.66847932261567	40.0	37.0	41.0	33.0	41.0
39	37.571656787320805	40.0	37.0	41.0	33.0	41.0
40	37.504694853095735	40.0	37.0	41.0	33.0	41.0
41	37.48592285949567	40.0	37.0	41.0	33.0	41.0
42	37.41232111459794	40.0	36.0	41.0	33.0	41.0
43	37.34018581578173	40.0	36.0	41.0	33.0	41.0
44	37.29953583148482	40.0	36.0	41.0	33.0	41.0
45	37.270926531793194	40.0	36.0	41.0	33.0	41.0
46	37.18919874672028	40.0	35.0	41.0	33.0	41.0
47	37.08197755076302	40.0	35.0	41.0	33.0	41.0
48	37.06705343254719	40.0	35.0	41.0	33.0	41.0
49	36.98971262108072	39.0	35.0	41.0	33.0	41.0
50	36.891547286085576	39.0	35.0	41.0	32.0	41.0
51	36.26220451606043	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	0.0
14	8.0
15	4.0
16	10.0
17	34.0
18	50.0
19	134.0
20	306.0
21	617.0
22	966.0
23	1341.0
24	1929.0
25	2468.0
26	2852.0
27	2987.0
28	3146.0
29	3154.0
30	3580.0
31	4536.0
32	5895.0
33	8172.0
34	15272.0
35	21242.0
36	23993.0
37	36946.0
38	69451.0
39	194643.0
40	638.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.79959641821163	22.384940859935853	23.477232991822028	13.33822973003049
2	33.12560741284785	27.971284364420505	22.008808568199633	16.89429965453201
3	32.48685020735498	27.980434196632366	23.37633754472908	16.156378051283575
4	29.471856847165657	28.32540760029576	23.78288684625067	18.419848706287915
5	30.154632164380445	31.605498801866567	18.394130258989712	19.84573877476328
6	39.243383063907864	29.831915109340496	16.061664923252692	14.863036903498944
7	76.85092450399254	6.206553752791317	11.537196540868838	5.405325202347303
8	68.19295759671002	9.935234025505775	13.614703038486173	8.257105339298034
9	34.54210035634887	27.187613600112766	18.203467539115533	20.06681850442283
10	27.38544781009894	28.18717094606792	26.324561859048075	18.102819384785064
11	27.60430190489615	21.084428222039225	30.84853565590696	20.462734217157667
12	25.362098427465323	21.30105668197409	31.67498806812421	21.66185682243638
13	21.01271332091924	27.79447003924536	30.662571498519952	20.530245141315447
14	18.73316863635352	26.236031050079255	34.86481741138882	20.165982902178403
15	20.545329999826894	24.487176633801457	32.74329280204956	22.224200564322082
16	20.344528276690927	23.842237109246522	28.997302035961315	26.815932578101236
17	22.43266836309502	24.58906125194434	28.73294607286729	24.24532431209336
18	22.837733908041713	26.4615620494635	29.737696566834575	20.96300747566021
19	25.281728279658438	23.108766775722774	28.216351492040882	23.393153452577902
20	22.976217855032036	27.20344033691166	29.018321920772344	20.802019887283958
21	20.567091762925376	27.991809663706572	27.392619300210942	24.04847927315711
22	19.83955645570121	27.13691858380381	28.676810615783708	24.346714344711273
23	20.688759802066873	24.482230778551802	29.15087084146308	25.67813857791824
24	21.478612885436682	27.011046567700102	27.81450075300646	23.695839793856756
25	19.54503077558429	27.70865945066386	28.317741524658796	24.428568249093054
26	19.020028240833476	27.680220782978342	28.820240418023683	24.479510558164495
27	22.62258920468175	26.244933589528635	30.835923725020344	20.296553480769276
28	20.489194542743316	28.101113064723936	27.32461379052819	24.085078602004558
29	22.139131854028026	24.467887798327805	28.945370555839943	24.447609791804222
30	21.158121465259075	27.81548992405639	27.36863190225012	23.657756708434412
31	24.212434374683156	26.966781163215696	25.28271745070837	23.538067011392776
32	22.703453938013595	28.562314066754208	26.147994826635408	22.58623716859679
33	21.749893045880224	24.887790909023465	28.142163663296067	25.220152381800244
34	21.78649237472767	25.39424648658808	27.056548435996923	25.76271270268733
35	22.075577614069967	27.098093620094023	27.19404321193732	23.632285553898694
36	24.238647407506324	26.949223377079424	26.467002490238116	22.345126725176133
37	22.296162758204556	27.548413740575057	27.394597642310803	22.760825858909588
38	23.647864997935105	26.829286387275303	23.489597629946164	26.033250984843427
39	26.040422474955427	24.03562004950801	27.116640577280226	22.80731689825634
40	19.890251472010167	23.788821872550255	28.851893891621472	27.469032763818102
41	21.802319111526565	26.32629290838545	27.238803201946688	24.632584778141297
42	21.732582552506436	25.51888203887937	30.082917263260455	22.66561814535374
43	24.830666280889957	24.61527428476751	28.366705491630377	22.18735394271216
44	21.661609529673896	23.965141612200437	28.574431412115864	25.79881744600981
45	21.4640226124502	24.163470407711575	26.5817463320301	27.79076064780812
46	23.440139077449622	24.21070332534578	28.402810234952852	23.94634736225175
47	21.96305940714033	24.875673563661813	29.768608162144915	23.392658867052937
48	21.69944532233375	24.100658046040966	27.989584028844227	26.210312602781055
49	21.523125582683573	22.568679382460513	31.09508654010223	24.81310849475368
50	21.69944532233375	22.32361225484014	28.10259682129883	27.874345601527278
51	21.571594964130185	21.512491993896816	29.429075199256143	27.486837842716856
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	277.0
1	510.5
2	744.0
3	2008.0
4	3272.0
5	2199.0
6	1126.0
7	1170.5
8	1215.0
9	1211.5
10	1208.0
11	1261.5
12	1315.0
13	1328.5
14	1342.0
15	1347.5
16	1353.0
17	1310.5
18	1268.0
19	1316.5
20	1365.0
21	1487.5
22	1610.0
23	1707.5
24	1805.0
25	2577.5
26	4080.5
27	4811.0
28	5323.5
29	5836.0
30	6439.0
31	7042.0
32	7598.0
33	8154.0
34	8685.0
35	9216.0
36	9815.5
37	10415.0
38	11417.0
39	12419.0
40	13391.0
41	14363.0
42	16564.0
43	18765.0
44	21786.0
45	24807.0
46	32956.5
47	41106.0
48	42276.5
49	43447.0
50	41391.5
51	39336.0
52	33603.0
53	27870.0
54	24874.0
55	21878.0
56	19614.0
57	17350.0
58	16944.5
59	16539.0
60	16454.0
61	16369.0
62	14727.0
63	13085.0
64	11103.0
65	9121.0
66	7973.5
67	6826.0
68	5834.0
69	4842.0
70	4142.5
71	3443.0
72	2923.0
73	2403.0
74	2065.5
75	1363.5
76	999.0
77	781.0
78	563.0
79	413.0
80	263.0
81	158.5
82	54.0
83	54.5
84	55.0
85	37.5
86	20.0
87	11.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	404379.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.242138145081256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.75904187710516	27.059770544778456
2	10.229731984083628	7.824136474449647
3	4.130450481392795	4.738717737325218
4	2.592423363104771	3.965592495295552
5	1.9137046219908884	3.6592078261528034
6	1.5836379229454898	3.633702013264258
7	1.3659871872373093	3.6566789503118104
8	1.169903902551157	3.579170132626482
9	0.988272105465761	3.4014274543936747
>10	5.149501101267993	29.56254661396892
>50	0.06548005932793623	1.712703500426654
>100	0.04408558449801647	3.593007980339183
>500	0.0032415870954423877	0.9322184657794181
>1k	0.004538221933619343	2.6811198108879326
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGC	1855	0.4587280744054464	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1800	0.44512697246889676	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCG	1703	0.4211395745080729	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTC	1653	0.4087749363839368	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	1504	0.3719283147740115	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCC	1160	0.2868596044799557	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCT	1139	0.28166645646781857	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	954	0.2359172954085153	No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	862	0.213166361260105	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	794	0.19635045341128	No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	580	0.14342980223997784	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTAGCCAGT	570	0.14095687461515063	No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	486	0.12018428256660213	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	473	0.11696947665432676	No Hit
GAACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCT	442	0.10930340101736243	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	441	0.10905610825487971	No Hit
TGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCTT	427	0.10559400958012162	Illumina Single End Adapter 2 (95% over 21bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	424	0.10485213129267346	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCT	421	0.10411025300522529	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	407	0.10064815433046721	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.28735419000492113	0.0
2	0.0	0.0	0.0	1.1518896876445117	0.0
3	0.0	0.0	0.0	1.6039408574629246	0.0
4	0.0	0.0	0.0	2.269900266828891	0.0
5	0.0	0.0	0.0	4.250220708790516	0.0
6	0.0	0.0	0.0	5.101896982781005	0.0
7	0.0	0.0	0.0	6.761973297327507	0.0
8	0.0	0.0	0.0	8.299145108920097	0.0
9	0.0	0.0	0.0	9.849670729686753	0.0
10	0.0	0.0	0.0	11.37002663343052	0.0
11	0.0	0.0	0.0	13.557578410352665	0.0
12	0.0	0.0	0.0	15.453819313070165	0.0
13	0.0	0.0	0.0	16.03619376871697	0.0
14	0.0	0.0	0.0	16.400208715091534	0.0
15	0.0	0.0	0.0	17.239025765432924	0.0
16	0.0	0.0	0.0	18.46139389038501	0.0
17	0.0	0.0	0.0	20.569070105025236	0.0
18	0.0	0.0	0.0	21.319850931922776	0.0
19	0.0	0.0	0.0	22.52095187930135	0.0
20	0.0	0.0	0.0	23.243541331275857	0.0
21	0.0	0.0	0.0	24.066778937580835	0.0
22	0.0	0.0	0.0	24.848471359788714	0.0
23	0.0	0.0	0.0	25.44444691737207	0.0
24	0.0	0.0	0.0	25.94521476139958	0.0
25	0.0	0.0	0.0	26.4343598455904	0.0
26	0.0	0.0	0.0	27.050860702459822	0.0
27	0.0	0.0	0.0	27.5434678853254	0.0
28	0.0	0.0	0.0	28.060062466151802	0.0
29	0.0	0.0	0.0	28.592731076539582	0.0
30	0.0	0.0	0.0	29.14048454543881	0.0
31	0.0	0.0	0.0	29.66425061637721	0.0
32	0.0	0.0	0.0	30.171942657754236	0.0
33	0.0	0.0	0.0	30.652679788020645	0.0
34	0.0	0.0	0.0	31.158146194535323	0.0
35	0.0	0.0	0.0	31.65965591685028	0.0
36	0.0	0.0	0.0	32.178228839776544	0.0
37	0.0	0.0	0.0	32.662428068717716	0.0
38	0.0	0.0	0.0	33.19460209358053	0.0
39	0.0	0.0	0.0	33.693638888270655	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	20	7.0278556E-4	45.000004	1
GGCGTAA	20	7.0278556E-4	45.000004	7
TAACGGC	20	7.0278556E-4	45.000004	3
GCGCAAA	20	7.0278556E-4	45.000004	10
CATACGA	65	0.0	45.000004	17
TTAGTCA	40	6.7957444E-9	45.000004	32
CGACAGG	35	1.2093187E-7	45.0	2
TAACGGA	35	1.2093187E-7	45.0	3
CCTAACG	25	3.886305E-5	45.0	1
GCCGATT	25	3.886305E-5	45.0	8
TACGAAT	50	2.1827873E-11	45.0	12
TTAGTAG	25	3.886305E-5	45.0	1
TATCGCG	25	3.886305E-5	45.0	32
AAGCACG	30	2.1619999E-6	44.999996	1
CGAATAT	55	6.002665E-11	40.909092	14
GGCCGAT	105	0.0	40.714287	7
ACGGAGT	40	3.4508048E-7	39.375004	5
GTCGTTT	40	3.4508048E-7	39.375004	8
TTTAGCG	35	6.2382496E-6	38.571426	1
CGATTAG	35	6.2382496E-6	38.571426	9
>>END_MODULE