##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551674_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	473186
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.521069938671054	34.0	31.0	34.0	31.0	34.0
2	32.656338099605655	34.0	31.0	34.0	31.0	34.0
3	32.70010524402666	34.0	31.0	34.0	31.0	34.0
4	36.13862836178585	37.0	37.0	37.0	35.0	37.0
5	36.151771185115365	37.0	35.0	37.0	35.0	37.0
6	36.182425092881026	37.0	37.0	37.0	35.0	37.0
7	36.2721910622884	37.0	37.0	37.0	35.0	37.0
8	35.95650124898032	37.0	37.0	37.0	35.0	37.0
9	37.700785737532385	39.0	37.0	39.0	35.0	39.0
10	37.77082373527534	39.0	37.0	39.0	35.0	39.0
11	37.800423089440514	39.0	38.0	39.0	35.0	39.0
12	37.766172287430315	39.0	38.0	39.0	35.0	39.0
13	37.74018673418064	39.0	38.0	39.0	35.0	39.0
14	39.144321683228156	40.0	39.0	41.0	35.0	41.0
15	39.16223218776549	40.0	39.0	41.0	35.0	41.0
16	39.149953717988275	40.0	39.0	41.0	35.0	41.0
17	39.12157798413309	40.0	39.0	41.0	35.0	41.0
18	39.043477617681	40.0	38.0	41.0	35.0	41.0
19	39.0310427611975	40.0	38.0	41.0	35.0	41.0
20	38.91429374495441	40.0	38.0	41.0	35.0	41.0
21	38.89058425228134	40.0	38.0	41.0	35.0	41.0
22	38.87146069410338	40.0	38.0	41.0	35.0	41.0
23	38.87580148186971	40.0	38.0	41.0	35.0	41.0
24	38.80602765086034	40.0	38.0	41.0	35.0	41.0
25	38.770958988642946	40.0	38.0	41.0	35.0	41.0
26	38.727764557700354	40.0	38.0	41.0	35.0	41.0
27	38.63189739341401	40.0	38.0	41.0	35.0	41.0
28	38.58929046928692	40.0	38.0	41.0	35.0	41.0
29	38.54848621894984	40.0	38.0	41.0	35.0	41.0
30	38.42926037541263	40.0	38.0	41.0	35.0	41.0
31	38.351832049130785	40.0	38.0	41.0	35.0	41.0
32	38.228836017971794	40.0	38.0	41.0	34.0	41.0
33	38.11495056912081	40.0	38.0	41.0	34.0	41.0
34	37.99871931967556	40.0	38.0	41.0	34.0	41.0
35	37.906558942994934	40.0	37.0	41.0	34.0	41.0
36	37.85856301750263	40.0	37.0	41.0	34.0	41.0
37	37.77367250933037	40.0	37.0	41.0	33.0	41.0
38	37.72431348349275	40.0	37.0	41.0	33.0	41.0
39	37.60302502609967	40.0	37.0	41.0	33.0	41.0
40	37.52992058091321	40.0	37.0	41.0	33.0	41.0
41	37.498028259500494	40.0	36.0	41.0	33.0	41.0
42	37.442232018698775	40.0	36.0	41.0	33.0	41.0
43	37.369702400324606	40.0	36.0	41.0	33.0	41.0
44	37.30025824939876	40.0	36.0	41.0	33.0	41.0
45	37.264623213704546	40.0	35.0	41.0	33.0	41.0
46	37.178893711986404	40.0	35.0	41.0	33.0	41.0
47	37.09070428964509	40.0	35.0	41.0	33.0	41.0
48	37.03452553541314	39.0	35.0	41.0	33.0	41.0
49	36.94102953172748	39.0	35.0	41.0	32.0	41.0
50	36.84400637381495	39.0	35.0	41.0	32.0	41.0
51	36.1617651409805	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	4.0
15	8.0
16	16.0
17	39.0
18	86.0
19	147.0
20	323.0
21	556.0
22	882.0
23	1312.0
24	1982.0
25	2639.0
26	3228.0
27	3554.0
28	3748.0
29	3957.0
30	4384.0
31	5573.0
32	7152.0
33	9718.0
34	18284.0
35	26324.0
36	28333.0
37	43407.0
38	83155.0
39	223700.0
40	674.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.528853347309514	22.568292383967403	24.167452122421203	13.735402146301878
2	33.53755182951313	26.66224275443483	22.166758948912264	17.63344646713977
3	32.31667885355864	26.22034464248731	24.445778192930476	17.01719831102357
4	29.461142130156006	29.229732071532126	22.74285376152295	18.566272036788916
5	29.142874049528096	32.492508231435416	18.594802044016518	19.769815675019974
6	40.0398574767639	28.790158626840185	15.733981985942103	15.436001910453816
7	78.5896032426995	5.800467469451759	9.884696504123113	5.725232783725639
8	69.70874032621421	8.827606902993749	13.289277366616933	8.174375404175102
9	35.75338239085688	26.93866682446226	18.640450055580683	18.667500729100183
10	27.071172858030458	27.261161572827596	27.10773353395916	18.559932035182783
11	27.066734856906166	20.86114128482246	30.873694488002602	21.198429370268773
12	24.7076202592638	20.978853981309676	32.125844805214015	22.187680954212507
13	20.892629959466255	27.11513020249965	30.064076282899325	21.928163555134766
14	18.232999285693154	25.25645306496811	35.86433241896421	20.646215230374526
15	20.930669969103057	24.059249428343186	32.9420143453103	22.06806625724345
16	20.47228785297959	24.014658083713382	29.58794216227868	25.925111901028348
17	21.583901467921702	25.089499689339924	28.71724860836965	24.609350234368726
18	23.216029214727403	26.66583542201164	29.543139484262003	20.574995878998955
19	25.801059202935	23.106558520328157	28.60862324751789	22.483759029218955
20	22.895225133457036	27.582810987645452	28.0054777613877	21.516486117509817
21	20.924329967496924	27.32223692163335	27.645788336933048	24.107644773936677
22	20.49088519102425	27.40655894299493	27.87825506249128	24.224300803489538
23	20.644947230053297	24.0913721031476	29.496857472537226	25.766823194261878
24	21.967894231866538	26.31692400028741	28.10543845337774	23.609743314468307
25	19.95177372111601	27.25904823895889	27.753357030850445	25.035821009074656
26	20.710037913209607	27.206637559014844	27.7683617013183	24.314962826457247
27	22.632115066802484	25.434184443326725	29.46896146547024	22.464739024400554
28	21.12974601953566	26.718880102116294	26.307202664491342	25.844171213856708
29	23.1274805256284	24.449793527281027	27.462350957128912	24.960374989961664
30	22.72510175702578	26.90083814821233	25.042372344067658	25.331687750694233
31	25.02926967408165	25.422349773661946	24.990595664284236	24.557784887972172
32	22.888462465077158	27.635433000976363	24.93903031788768	24.5370742160588
33	22.79315110759828	25.15543570604371	26.702396097940344	25.349017088417664
34	21.509723449129943	23.971968739565412	28.30028783607292	26.218019975231726
35	22.348505661621434	27.330267590334458	25.946879239876075	24.374347508168036
36	24.054388760445153	26.93084748914803	26.020845925280966	22.99391782512585
37	21.516908784283558	26.640475415587105	27.89114639909042	23.951469401038917
38	21.95162156107746	26.614904075775698	27.134150207318054	24.299324155828785
39	24.48614286982286	25.589514482677	29.170981389981954	20.753361257518186
40	20.9310926358768	24.86738829973837	30.496675725824517	23.704843338560313
41	23.36248325182909	26.047262598639858	26.266837987598958	24.323416161932094
42	22.28172431137016	24.660281580604668	27.765614367288972	25.292379740736198
43	24.410485517322996	23.200179210712065	28.401939195157926	23.987396076807006
44	20.753572590905055	24.3270088295089	28.988389343725302	25.93102923586074
45	21.775158183040073	25.916869898940377	26.809753458470876	25.498218459548678
46	22.803295110168094	24.515518210597946	29.852531562641328	22.828655116592632
47	21.86138220488349	24.11081477473974	29.620487503856836	24.40731551651993
48	21.238371380387417	23.82826203649305	29.221067402670407	25.71229918044913
49	21.26373138681195	22.793996441145765	30.820861141284823	25.12141103075746
50	21.264154053585692	22.801604443073124	28.585587908348938	27.348653594992246
51	21.423499427286522	21.9074528832214	29.6346468407772	27.034400848714878
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	290.0
1	576.5
2	863.0
3	2440.5
4	4018.0
5	2707.5
6	1397.0
7	1358.0
8	1319.0
9	1367.5
10	1416.0
11	1444.5
12	1473.0
13	1469.0
14	1465.0
15	1497.0
16	1529.0
17	1484.5
18	1440.0
19	1519.5
20	1599.0
21	1583.0
22	1567.0
23	1850.5
24	2134.0
25	3013.5
26	4837.5
27	5782.0
28	6220.0
29	6658.0
30	7545.5
31	8433.0
32	8667.5
33	8902.0
34	10037.0
35	11172.0
36	11780.0
37	12388.0
38	13147.5
39	13907.0
40	15352.0
41	16797.0
42	19931.5
43	23066.0
44	25959.0
45	28852.0
46	35054.0
47	41256.0
48	45246.0
49	49236.0
50	47290.0
51	45344.0
52	38865.5
53	32387.0
54	29198.5
55	26010.0
56	23972.0
57	21934.0
58	21719.5
59	21505.0
60	20922.5
61	20340.0
62	18428.0
63	16516.0
64	14250.0
65	11984.0
66	10041.5
67	8099.0
68	7023.5
69	5948.0
70	5051.0
71	4154.0
72	3754.0
73	3354.0
74	2745.5
75	1668.0
76	1199.0
77	952.0
78	705.0
79	540.5
80	376.0
81	294.0
82	212.0
83	156.5
84	101.0
85	59.0
86	17.0
87	12.0
88	7.0
89	5.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	473186.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.95742871999135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.39004884454259	26.014352127688923
2	9.937202922421394	7.345069373629568
3	3.9618945172086715	4.392643026475921
4	2.4294719981733626	3.5914815279882792
5	1.9295737613269839	3.56560423721038
6	1.5774248532402564	3.4978539944861753
7	1.3180227310320936	3.4097511793412845
8	1.2466042767204861	3.685703095914699
9	1.0693456159927803	3.5568237942079577
>10	6.037915429081806	33.87777147058085
>50	0.05611460852202198	1.4571982066056317
>100	0.03779147104544329	2.6852146447311687
>500	0.006298578507573881	1.5759156323834043
>1k	0.0022903921845723206	1.3446176887557608
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2275	0.48078345513180865	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	1656	0.3499680886585825	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCC	1311	0.2770580701880444	No Hit
CTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGCT	1112	0.23500272620069063	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTC	842	0.17794271174548698	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	787	0.16631937546757514	No Hit
CCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	755	0.15955670708769915	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	732	0.15469603918966326	No Hit
TCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	724	0.15300537209469428	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCG	688	0.14539737016733378	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	673	0.14222736936426691	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC	652	0.1377893682399733	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	553	0.11686736293973195	No Hit
TGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGCTT	532	0.11242936181543833	Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	509	0.10756869391740245	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1133338687112467E-4	0.0	0.0	0.240708727646211	0.0
2	2.1133338687112467E-4	0.0	0.0	1.0268689268067948	0.0
3	2.1133338687112467E-4	0.0	0.0	1.4905343776020423	0.0
4	2.1133338687112467E-4	0.0	0.0	2.0839585279361605	0.0
5	2.1133338687112467E-4	0.0	0.0	3.7659609540434418	0.0
6	2.1133338687112467E-4	0.0	0.0	4.5916404965489255	0.0
7	2.1133338687112467E-4	0.0	0.0	6.233700912537564	0.0
8	2.1133338687112467E-4	0.0	0.0	7.818489980684128	0.0
9	2.1133338687112467E-4	0.0	0.0	9.500069740017668	0.0
10	2.1133338687112467E-4	0.0	0.0	10.749261389812885	0.0
11	2.1133338687112467E-4	0.0	0.0	12.888800598496152	0.0
12	2.1133338687112467E-4	0.0	0.0	14.32016162777428	0.0
13	2.1133338687112467E-4	0.0	0.0	14.818908420790134	0.0
14	2.1133338687112467E-4	0.0	0.0	15.140135168834243	0.0
15	2.1133338687112467E-4	0.0	0.0	15.856555350327355	0.0
16	2.1133338687112467E-4	0.0	0.0	16.949994293998554	0.0
17	2.1133338687112467E-4	0.0	0.0	18.76577075399526	0.0
18	2.1133338687112467E-4	0.0	0.0	19.458098929385063	0.0
19	2.1133338687112467E-4	0.0	0.0	20.35964715777728	0.0
20	2.1133338687112467E-4	0.0	0.0	20.973359313251027	0.0
21	2.1133338687112467E-4	0.0	0.0	21.772410849010747	0.0
22	2.1133338687112467E-4	0.0	0.0	22.50362436758484	0.0
23	2.1133338687112467E-4	0.0	0.0	23.0784511798743	0.0
24	2.1133338687112467E-4	0.0	0.0	23.607629980599594	0.0
25	2.1133338687112467E-4	0.0	0.0	24.073831432037295	0.0
26	2.1133338687112467E-4	0.0	0.0	24.59878356502517	0.0
27	4.2266677374224934E-4	0.0	0.0	25.084216354668143	0.0
28	4.2266677374224934E-4	0.0	0.0	25.594163817188168	0.0
29	4.2266677374224934E-4	0.0	0.0	26.133275287096406	0.0
30	4.2266677374224934E-4	0.0	0.0	26.67111875668342	0.0
31	4.2266677374224934E-4	0.0	0.0	27.202833558051168	0.0
32	4.2266677374224934E-4	0.0	0.0	27.679813012219295	0.0
33	4.2266677374224934E-4	0.0	0.0	28.177291804913924	0.0
34	4.2266677374224934E-4	0.0	0.0	28.67477059760855	0.0
35	4.2266677374224934E-4	0.0	0.0	29.166332055470786	0.0
36	4.2266677374224934E-4	0.0	0.0	29.664867515099772	0.0
37	4.2266677374224934E-4	0.0	0.0	30.149877637969002	0.0
38	4.2266677374224934E-4	0.0	0.0	30.691947775293436	0.0
39	6.340001606133741E-4	0.0	0.0	31.186045233798126	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACCGG	25	3.887314E-5	45.000004	2
TAGGCAC	25	3.887314E-5	45.000004	5
TAGTGCG	25	3.887314E-5	45.000004	1
CGTTCGC	20	7.029074E-4	45.0	45
GTATGCG	35	1.2099008E-7	45.0	1
TCTCGTA	35	1.2099008E-7	45.0	27
CAATGCG	20	7.029074E-4	45.0	1
TCGAAAG	20	7.029074E-4	45.0	1
CGAAAGG	60	0.0	44.999996	2
TAAACGG	30	2.1627839E-6	44.999996	2
ACGGGAT	65	0.0	41.538464	4
GTACGGA	65	0.0	41.538464	3
CGAATAT	165	0.0	40.90909	14
CTACGAA	175	0.0	39.857143	11
GCTACGA	175	0.0	39.857143	10
CGTACGG	40	3.4524783E-7	39.375	30
TCGTACG	40	3.4524783E-7	39.375	29
CGTTTTT	1320	0.0	39.375	1
TACACGG	40	3.4524783E-7	39.375	2
GGACTAA	225	0.0	39.0	7
>>END_MODULE