##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551673_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	401200
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.571348454636095	34.0	31.0	34.0	31.0	34.0
2	32.706084247258225	34.0	31.0	34.0	31.0	34.0
3	32.74152043868395	34.0	31.0	34.0	31.0	34.0
4	36.16621635094716	37.0	37.0	37.0	35.0	37.0
5	36.17687437686939	37.0	37.0	37.0	35.0	37.0
6	36.20635343968096	37.0	37.0	37.0	35.0	37.0
7	36.288870887337985	37.0	37.0	37.0	35.0	37.0
8	35.9939556331007	37.0	37.0	37.0	35.0	37.0
9	37.7109072781655	39.0	37.0	39.0	35.0	39.0
10	37.780421236291126	39.0	37.0	39.0	35.0	39.0
11	37.81025672981057	39.0	38.0	39.0	35.0	39.0
12	37.784710867397806	39.0	38.0	39.0	35.0	39.0
13	37.76293369890329	39.0	38.0	39.0	35.0	39.0
14	39.19701395812562	41.0	39.0	41.0	35.0	41.0
15	39.18482801595214	41.0	39.0	41.0	35.0	41.0
16	39.19005732801595	41.0	39.0	41.0	35.0	41.0
17	39.16008723828514	41.0	39.0	41.0	35.0	41.0
18	39.07429710867398	40.0	39.0	41.0	35.0	41.0
19	39.02080508474576	40.0	38.0	41.0	35.0	41.0
20	38.869007976071785	40.0	38.0	41.0	35.0	41.0
21	38.84958624127617	40.0	38.0	41.0	35.0	41.0
22	38.837639581256234	40.0	38.0	41.0	35.0	41.0
23	38.815665503489534	40.0	38.0	41.0	35.0	41.0
24	38.764489032901295	40.0	38.0	41.0	35.0	41.0
25	38.738908275174474	40.0	38.0	41.0	35.0	41.0
26	38.64733549351944	40.0	38.0	41.0	35.0	41.0
27	38.55571784646062	40.0	38.0	41.0	35.0	41.0
28	38.50521186440678	40.0	38.0	41.0	35.0	41.0
29	38.43535144566301	40.0	38.0	41.0	35.0	41.0
30	38.32892821535394	40.0	38.0	41.0	35.0	41.0
31	38.19184945164506	40.0	38.0	41.0	34.0	41.0
32	37.98570787637089	40.0	37.0	41.0	34.0	41.0
33	37.82578265204387	40.0	37.0	41.0	33.0	41.0
34	37.657215852442675	40.0	37.0	41.0	33.0	41.0
35	37.56050099700897	40.0	37.0	41.0	33.0	41.0
36	37.49823778664008	40.0	37.0	41.0	33.0	41.0
37	37.366168993020935	40.0	37.0	41.0	33.0	41.0
38	37.31324277168495	40.0	37.0	41.0	33.0	41.0
39	37.23382103688933	40.0	36.0	41.0	32.0	41.0
40	37.15163509471585	40.0	36.0	41.0	32.0	41.0
41	37.11569042871386	40.0	36.0	41.0	32.0	41.0
42	37.03541375872383	40.0	36.0	41.0	32.0	41.0
43	36.924486540378865	40.0	35.0	41.0	31.0	41.0
44	36.887853938185444	40.0	35.0	41.0	31.0	41.0
45	36.83913010967099	40.0	35.0	41.0	31.0	41.0
46	36.77566799601196	40.0	35.0	41.0	31.0	41.0
47	36.68421236291127	39.0	35.0	41.0	31.0	41.0
48	36.62108923230309	39.0	35.0	41.0	31.0	41.0
49	36.543786141575275	39.0	35.0	41.0	31.0	41.0
50	36.44416749750748	39.0	35.0	41.0	31.0	41.0
51	35.74864406779661	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	2.0
11	2.0
12	1.0
13	2.0
14	5.0
15	7.0
16	18.0
17	27.0
18	73.0
19	170.0
20	325.0
21	623.0
22	1025.0
23	1529.0
24	2183.0
25	3038.0
26	3869.0
27	4062.0
28	3869.0
29	3626.0
30	4056.0
31	4693.0
32	6001.0
33	8220.0
34	14777.0
35	21822.0
36	23493.0
37	36054.0
38	68683.0
39	188271.0
40	673.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.632103688933206	21.943918245264207	22.215353938185444	14.208624127617147
2	34.5024925224327	26.418993020937187	22.119641076769692	16.958873379860417
3	32.01370887337986	27.24476570289133	24.913509471585243	15.82801595214357
4	28.13384845463609	28.531156530408776	24.941924227318047	18.39307078763709
5	29.450398803589234	31.889332003988034	19.811066799601196	18.849202392821535
6	38.96385842472582	29.188434695912264	16.964855433698904	14.882851445663011
7	79.16500498504486	5.209122632103689	10.858175473579262	4.767696909272184
8	71.26869391824526	8.4481555333998	13.077766699900298	7.205383848454636
9	34.90528414755733	25.314307078763708	19.97457627118644	19.805832502492525
10	28.126620139581256	27.920737786640082	25.67073778664008	18.281904287138584
11	26.66475573280159	22.295363908275174	30.876121635094716	20.163758723828515
12	25.064307078763708	22.13359920239282	31.78614157527418	21.01595214356929
13	21.795613160518446	26.83898305084746	31.212113659022933	20.153290129611168
14	19.92622133599202	25.827018943170486	34.774177467597205	19.47258225324028
15	21.452143569292122	24.278165503489532	32.621385842472584	21.64830508474576
16	21.253489531405783	23.771684945164505	29.14581256231306	25.82901296111665
17	22.67447657028913	24.85169491525424	29.096211365902292	23.377617148554336
18	24.15927218344965	25.874875373878364	29.700648055832502	20.265204386839482
19	25.679461615154537	23.11714855433699	28.798105682951146	22.405284147557328
20	24.080259222333	25.956879361914257	29.534147557328016	20.428713858424725
21	21.613659022931206	27.033898305084747	28.778664007976072	22.573778664007975
22	20.588733798604185	26.691425722831507	29.477567298105683	23.242273180458625
23	21.345463609172484	24.493519441674973	29.56455633100698	24.596460618145564
24	21.92173479561316	26.680458624127617	28.595962113659024	22.801844466600198
25	20.144317048853438	27.907527417746756	28.285393818544367	23.662761714855435
26	20.44940179461615	27.480807577268195	28.798354935194414	23.271435692921237
27	23.11764705882353	26.652043868394813	29.75199401794616	20.478315054835495
28	21.014705882352942	28.30059820538385	27.455633100697906	23.229062811565303
29	21.86714855433699	26.45239282153539	28.573280159521435	23.107178464606182
30	23.280159521435692	27.52367896311067	26.46261216350947	22.73354935194417
31	23.48978065802592	28.20962113659023	25.609670987038886	22.690927218344964
32	23.634596211365903	29.738035892323033	24.84172482552343	21.785643070787636
33	23.27941176470588	27.253988035892323	27.238534396809573	22.228065802592223
34	22.558325024925225	27.30259222333001	26.058075772681953	24.08100697906281
35	23.85219341974078	28.714855433698904	25.690428713858427	21.742522432701893
36	25.434695912263212	29.489531405782653	24.561814556331008	20.51395812562313
37	23.799351944167498	28.109920239282154	26.44740777666999	21.643320039880358
38	23.782652043868392	27.120139581256232	25.060319042871388	24.036889332003987
39	24.914755732801595	25.58998005982054	28.19416749750748	21.30109670987039
40	22.166001994017943	24.8160518444666	28.4314556331007	24.586490528414757
41	24.72731804586241	26.770189431704882	25.156281156530408	23.346211365902292
42	25.032153539381852	25.669990029910267	26.776919242273177	22.520937188434694
43	24.18519441674975	27.122632103688932	27.415254237288135	21.27691924227318
44	22.279910269192424	26.30333998005982	27.402791625124628	24.01395812562313
45	22.128364905284148	25.42123629112662	27.454386839481554	24.99601196410768
46	23.814057826520436	25.456380857427718	27.84322033898305	22.886340977068794
47	23.559820538384844	24.844217347956132	29.24127617148554	22.35468594217348
48	22.3703888334995	25.198654037886342	28.112911266201397	24.318045862412763
49	22.576021934197406	23.806081754735793	29.674227318045865	23.943668993020935
50	22.293868394815554	23.913260219341975	27.632602193419743	26.16026919242273
51	22.225074775672983	23.44790628115653	28.494017946161517	25.833000997008977
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	193.0
1	437.0
2	681.0
3	2616.0
4	4551.0
5	3119.5
6	1688.0
7	1673.0
8	1658.0
9	1646.0
10	1634.0
11	1657.5
12	1681.0
13	1638.0
14	1595.0
15	1542.0
16	1489.0
17	1490.0
18	1491.0
19	1497.5
20	1504.0
21	1627.5
22	1751.0
23	1873.5
24	1996.0
25	2289.0
26	3190.5
27	3799.0
28	4646.5
29	5494.0
30	5740.0
31	5986.0
32	6790.0
33	7594.0
34	8561.5
35	9529.0
36	10324.0
37	11119.0
38	12032.5
39	12946.0
40	14609.5
41	16273.0
42	18333.0
43	20393.0
44	22546.0
45	24699.0
46	29837.5
47	34976.0
48	36854.5
49	38733.0
50	38219.5
51	37706.0
52	32997.0
53	28288.0
54	25750.0
55	23212.0
56	20809.0
57	18406.0
58	17886.0
59	17366.0
60	16951.0
61	16536.0
62	14948.5
63	13361.0
64	11468.0
65	9575.0
66	8177.0
67	6779.0
68	5714.0
69	4649.0
70	3940.5
71	3232.0
72	2887.5
73	2543.0
74	2072.0
75	1241.0
76	881.0
77	703.5
78	526.0
79	394.0
80	262.0
81	218.0
82	174.0
83	111.0
84	48.0
85	28.5
86	9.0
87	8.0
88	7.0
89	4.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	401200.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.58285359019819
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.38299867609723	29.375086376915633
2	9.209498333850638	7.474954450436693
3	3.838363536119369	4.673152362368634
4	2.421077915726397	3.930170023375462
5	1.8436380520384825	3.741004656959797
6	1.6242022275685182	3.9548856721372165
7	1.3695958605572403	3.8907475800715186
8	1.1912481956025938	3.8675400889382274
9	1.0560341233646928	3.857119039328636
>10	4.9950272021311415	29.68244062771388
>50	0.03877387610321	1.0919968945803264
>100	0.024618334033784127	2.073095659884883
>500	0.0018463750525338095	0.610189481578165
>1k	0.003077291754223016	1.7776170857109295
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2535	0.6318544366899302	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCG	1181	0.2943668993020937	No Hit
GCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	1165	0.29037886340977065	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGC	1119	0.278913260219342	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTC	1117	0.2784147557328016	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCC	922	0.22981056829511465	No Hit
CTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGCT	911	0.22706879361914256	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	610	0.15204386839481554	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAATCGGT	431	0.10742771684945165	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2330508474576271	0.0
2	0.0	0.0	0.0	0.848703888334995	0.0
3	0.0	0.0	0.0	1.1807078763708874	0.0
4	0.0	0.0	0.0	1.6221335992023929	0.0
5	0.0	0.0	0.0	2.935692921236291	0.0
6	0.0	0.0	0.0	3.527417746759721	0.0
7	0.0	0.0	0.0	4.715852442671984	0.0
8	0.0	0.0	0.0	5.807078763708874	0.0
9	2.492522432701894E-4	0.0	0.0	7.030408773678963	0.0
10	2.492522432701894E-4	0.0	0.0	8.26370887337986	0.0
11	2.492522432701894E-4	0.0	0.0	10.071286141575275	0.0
12	2.492522432701894E-4	0.0	0.0	11.548853439680958	0.0
13	2.492522432701894E-4	0.0	0.0	12.038384845463609	0.0
14	2.492522432701894E-4	0.0	0.0	12.330508474576272	0.0
15	2.492522432701894E-4	0.0	0.0	13.01395812562313	0.0
16	2.492522432701894E-4	0.0	0.0	14.0259222333001	0.0
17	2.492522432701894E-4	0.0	0.0	15.691425722831505	0.0
18	2.492522432701894E-4	0.0	0.0	16.3327517447657	0.0
19	2.492522432701894E-4	0.0	0.0	17.246011964107677	0.0
20	2.492522432701894E-4	0.0	0.0	17.863659022931206	0.0
21	2.492522432701894E-4	0.0	0.0	18.60219341974078	0.0
22	2.492522432701894E-4	0.0	0.0	19.337487537387837	0.0
23	2.492522432701894E-4	0.0	0.0	19.890578265204386	0.0
24	2.492522432701894E-4	0.0	0.0	20.389830508474578	0.0
25	2.492522432701894E-4	0.0	0.0	20.837986041874377	0.0
26	2.492522432701894E-4	0.0	0.0	21.403290129611168	0.0
27	2.492522432701894E-4	0.0	0.0	21.878115653040876	0.0
28	2.492522432701894E-4	0.0	0.0	22.385593220338983	0.0
29	2.492522432701894E-4	0.0	0.0	22.876121635094716	0.0
30	2.492522432701894E-4	0.0	0.0	23.387836490528414	0.0
31	2.492522432701894E-4	0.0	0.0	23.884097706879363	0.0
32	2.492522432701894E-4	0.0	0.0	24.360667996011966	0.0
33	2.492522432701894E-4	0.0	0.0	24.807328015952145	0.0
34	2.492522432701894E-4	0.0	0.0	25.283649052841476	0.0
35	2.492522432701894E-4	0.0	0.0	25.76296111665005	0.0
36	2.492522432701894E-4	0.0	0.0	26.231306081754735	0.0
37	2.492522432701894E-4	0.0	0.0	26.708873379860417	0.0
38	2.492522432701894E-4	0.0	0.0	27.230558325024926	0.0
39	2.492522432701894E-4	0.0	0.0	27.720837487537388	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGG	35	1.2092823E-7	45.000004	2
GTCGAAT	25	3.8862512E-5	45.0	43
CGACAGG	25	3.8862512E-5	45.0	2
ATCGTTG	20	7.0277904E-4	45.0	22
CGTTTTT	1570	0.0	41.273888	1
TAAACGG	40	3.4507138E-7	39.375	2
ATTGACG	40	3.4507138E-7	39.375	1
CGAAAGG	35	6.238126E-6	38.57143	2
CGCGAGG	35	6.238126E-6	38.57143	2
TTATACG	35	6.238126E-6	38.57143	1
TATTAGT	35	6.238126E-6	38.57143	30
TTACGGC	35	6.238126E-6	38.57143	3
CGGCACA	30	1.1385975E-4	37.500004	6
ATCGTGA	30	1.1385975E-4	37.500004	2
ACGGCAT	30	1.1385975E-4	37.500004	5
CGGATAA	30	1.1385975E-4	37.500004	6
ATGCGGT	30	1.1385975E-4	37.500004	3
GGACCGA	205	0.0	37.317074	7
GCGATTG	145	0.0	37.241383	8
CGGACAG	55	2.739398E-9	36.81818	6
>>END_MODULE