FastQCFastQC Report
Sat 18 Jun 2016
SRR3551669_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551669_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465543
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49741.0684297691083315No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC16970.36452057060250076Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCC16530.3550692417241802No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTC11400.24487533912012424No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT10770.23134275458980158Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGC8720.18730815413398977No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCG8280.17785682525566918No Hit
TCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC7230.15530251770513143Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC7210.15487291184702595Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCG7040.15122126205312936No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCT5880.12630412228301144No Hit
TGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTT5700.12243766956006212Illumina Single End Adapter 1 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC5270.11320114361079428Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTC5040.10826067624258125No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5000.10740146452637028No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGC4840.10396461766152643No Hit
CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG4800.10310540594531546No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGT4730.10160178544194629No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG502.1827873E-1145.01
TCGCATC207.0289575E-445.034
TTAATCG351.2098462E-745.019
GGCACGT207.0289575E-445.07
AACGGTA207.0289575E-445.04
TAGCACG207.0289575E-445.01
CGAGGTT207.0289575E-445.04
ATAAGCG351.2098462E-745.01
CGACTTA207.0289575E-445.011
CGGTATT207.0289575E-445.06
GACTGCG207.0289575E-445.01
GCGTCAT207.0289575E-445.036
TACAACG207.0289575E-445.01
TACCGGA207.0289575E-445.03
CGTTTTT34150.043.1551971
CGGACTG700.041.7857136
GTACGAG603.6379788E-1241.2499961
CATACGA556.002665E-1140.90909217
ACGGATG501.0786607E-940.55
TTGTGCG451.924127E-840.01
Produced by FastQC (version 0.11.5)