##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551669_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 465543 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47097045815317 34.0 31.0 34.0 31.0 34.0 2 32.62675198639008 34.0 31.0 34.0 31.0 34.0 3 32.64067766028057 34.0 31.0 34.0 31.0 34.0 4 36.11209920458475 37.0 37.0 37.0 35.0 37.0 5 36.1261473161448 37.0 35.0 37.0 35.0 37.0 6 36.164841915784365 37.0 37.0 37.0 35.0 37.0 7 36.29458073690293 37.0 37.0 37.0 35.0 37.0 8 36.03018840364907 37.0 37.0 37.0 35.0 37.0 9 37.6460241051847 39.0 37.0 39.0 35.0 39.0 10 37.74526091037777 39.0 37.0 39.0 35.0 39.0 11 37.816386456245716 39.0 38.0 39.0 35.0 39.0 12 37.8067998015221 39.0 38.0 39.0 35.0 39.0 13 37.77970241202209 39.0 38.0 39.0 35.0 39.0 14 39.188569047327526 40.0 39.0 41.0 36.0 41.0 15 39.20018344170141 41.0 39.0 41.0 36.0 41.0 16 39.20694543790799 41.0 39.0 41.0 35.0 41.0 17 39.173569358791774 41.0 39.0 41.0 35.0 41.0 18 39.0377559108396 40.0 39.0 41.0 35.0 41.0 19 38.951905624185095 40.0 38.0 41.0 35.0 41.0 20 38.765042112114244 40.0 38.0 41.0 35.0 41.0 21 38.75721254535027 40.0 38.0 41.0 35.0 41.0 22 38.7166835286966 40.0 38.0 41.0 35.0 41.0 23 38.72564295886739 40.0 38.0 41.0 35.0 41.0 24 38.665036312435156 40.0 38.0 41.0 35.0 41.0 25 38.6363193088501 40.0 38.0 41.0 35.0 41.0 26 38.59393869094799 40.0 38.0 41.0 35.0 41.0 27 38.51592011908674 40.0 38.0 41.0 35.0 41.0 28 38.4456559329643 40.0 38.0 41.0 35.0 41.0 29 38.380882109708445 40.0 38.0 41.0 35.0 41.0 30 38.23032029264751 40.0 37.0 41.0 34.0 41.0 31 38.049065285054226 40.0 37.0 41.0 34.0 41.0 32 37.82770871863609 40.0 37.0 41.0 33.0 41.0 33 37.539593549897646 40.0 37.0 41.0 33.0 41.0 34 37.306177947042485 40.0 37.0 41.0 32.0 41.0 35 37.16454333971298 40.0 37.0 41.0 32.0 41.0 36 37.03677426145383 40.0 37.0 41.0 31.0 41.0 37 36.96854425906952 40.0 37.0 41.0 31.0 41.0 38 36.92627748671981 40.0 37.0 41.0 31.0 41.0 39 36.906470508631855 40.0 36.0 41.0 31.0 41.0 40 36.82761850140589 40.0 36.0 41.0 31.0 41.0 41 36.79773726594536 40.0 36.0 41.0 31.0 41.0 42 36.717353713835244 40.0 36.0 41.0 30.0 41.0 43 36.66895646589037 40.0 36.0 41.0 30.0 41.0 44 36.666327278038764 40.0 36.0 41.0 30.0 41.0 45 36.6569296498927 40.0 36.0 41.0 30.0 41.0 46 36.559583110475295 40.0 35.0 41.0 30.0 41.0 47 36.48382211739839 40.0 35.0 41.0 30.0 41.0 48 36.41694107740853 40.0 35.0 41.0 30.0 41.0 49 36.378749116622956 40.0 35.0 41.0 30.0 41.0 50 36.26380162519896 39.0 35.0 41.0 29.0 41.0 51 35.637571609926475 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 0.0 11 1.0 12 2.0 13 0.0 14 3.0 15 4.0 16 15.0 17 37.0 18 68.0 19 168.0 20 337.0 21 610.0 22 1018.0 23 1663.0 24 2726.0 25 4341.0 26 6172.0 27 6515.0 28 5779.0 29 5135.0 30 5274.0 31 5879.0 32 7063.0 33 9474.0 34 16386.0 35 22606.0 36 26341.0 37 40239.0 38 79888.0 39 217191.0 40 606.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.76063650403937 22.916250485991625 23.96577759734332 15.357335412625686 2 36.212122188498164 25.12743183766054 22.307069379198055 16.353376594643247 3 29.824957093114925 25.91511417849694 28.14756102014207 16.11236770824607 4 27.158823137712307 27.598524733483266 27.354508606079353 17.888143522725073 5 27.587999389959684 31.14105463942106 23.133631050193003 18.137314920426256 6 36.1988044068969 28.262910193043396 20.66533059244796 14.872954807611757 7 76.7213769726964 4.639958070468249 13.574256298558888 5.064408658276464 8 68.9109276694097 7.359148349346892 16.45089712443319 7.279026856810219 9 30.62982366827554 26.386606607767703 23.843984336570415 19.139585387386344 10 24.495266817458322 28.393295570978406 30.308263683483588 16.803173928079683 11 22.879089579265504 22.61703000582116 34.25140105210475 20.252479362808593 12 22.706602827236154 22.815937518123995 35.559550890035936 18.917908764603915 13 20.31262418294336 27.52055127023712 33.31099382871185 18.855830718107672 14 18.08060694715633 26.059032140962273 37.85429058110636 18.00607033077503 15 19.456204904810082 25.50999585430347 34.68014769849402 20.35365154239243 16 19.189419666926575 25.231611258251117 32.60644022141886 22.972528853403446 17 20.892162485527653 24.814034364172592 32.43631630160909 21.85748684869067 18 21.370528608528105 27.9733558446803 32.10143853521586 18.55467701157573 19 21.82161475953886 24.60610512884954 32.0017699761354 21.570510135476205 20 19.896336106439147 29.34422813789489 31.840882582274894 18.91855317339107 21 19.247846063628923 27.171281707597366 30.98188566899298 22.59898655978073 22 17.709427485753196 28.70261178881435 30.854507532064705 22.733453193367744 23 17.738211078246263 25.8889082211525 32.27929536047153 24.093585340129696 24 19.310783321841377 28.039515146828542 31.28712063117693 21.362580900153155 25 17.750454845202267 27.641485319293814 31.269721593923656 23.33833824158026 26 17.59558193335524 28.330358312765952 30.430271747185543 23.64378800669326 27 19.95862895586444 26.959700822480414 32.65240804823615 20.429262173418998 28 18.6229843430145 28.38427384795819 28.925362426242046 24.067379382785266 29 18.8029891975607 27.21918276077613 29.460436522512424 24.517391519150756 30 20.252479362808593 29.576859710059004 27.211020249472124 22.959640677660282 31 20.34892587795327 30.49965309326958 25.97096294005065 23.180458088726496 32 18.788597401314163 32.1349477921481 25.449851034168702 23.62660377236904 33 18.432883750802826 29.432726944664616 27.321858560863337 24.81253074366922 34 18.192304470263757 31.479584055608186 26.113592084941672 24.21451938918639 35 20.021995819935 30.107852550677382 25.6025759167252 24.267575712662417 36 19.35825476916203 32.993515099571894 25.71577706033599 21.932453070930073 37 19.606137349288897 31.961172222544427 27.359019467589462 21.073670960577218 38 19.565109989839822 30.656459231478077 26.628904311739195 23.149526466942906 39 22.28086342185362 27.195984044438433 28.41456106095463 22.10859147275332 40 18.611599787774708 26.71718831557987 30.17981153191005 24.491400364735373 41 21.3582848415721 28.02039768614285 27.39360273916695 23.227714733118102 42 19.19135289328805 28.24357792942865 30.35036505757793 22.214704119705374 43 20.470074729939018 27.905692922028685 29.66471410804158 21.95951823999072 44 18.10487967813929 28.288686544529725 28.839870860479056 24.766562916851935 45 20.30102482477451 27.68444590510436 26.47037975009827 25.544149520022856 46 21.12479405769177 27.45138472708214 29.548505723424046 21.87531549180205 47 20.74008201175831 26.269968617292065 30.0468485188264 22.94310085212322 48 18.33450400929667 25.984710327510026 30.378933847141944 25.301851816051364 49 20.4528904956148 24.296359305155484 31.13203291640085 24.118717282828868 50 19.66864500164324 23.96835523249195 29.234893447007043 27.128106318857764 51 18.83993530135777 24.02484840283282 30.457766522104297 26.677449773705114 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 192.0 1 365.0 2 538.0 3 4286.0 4 8034.0 5 5367.5 6 2701.0 7 2769.0 8 2837.0 9 2876.5 10 2916.0 11 2844.0 12 2772.0 13 2666.5 14 2561.0 15 2499.0 16 2437.0 17 2421.0 18 2405.0 19 2430.5 20 2456.0 21 2476.0 22 2496.0 23 2633.5 24 2771.0 25 3340.5 26 4723.0 27 5536.0 28 5989.5 29 6443.0 30 7357.5 31 8272.0 32 9240.5 33 10209.0 34 11424.5 35 12640.0 36 13756.5 37 14873.0 38 16836.0 39 18799.0 40 21641.5 41 24484.0 42 28320.5 43 32157.0 44 40688.0 45 49219.0 46 51964.0 47 54709.0 48 51562.0 49 48415.0 50 40674.0 51 32933.0 52 27850.0 53 22767.0 54 20127.0 55 17487.0 56 15992.0 57 14497.0 58 13585.5 59 12674.0 60 12003.0 61 11332.0 62 10145.0 63 8958.0 64 7613.0 65 6268.0 66 5260.0 67 4252.0 68 3735.0 69 3218.0 70 2785.5 71 2353.0 72 2000.0 73 1647.0 74 1331.5 75 809.5 76 603.0 77 501.5 78 400.0 79 291.0 80 182.0 81 151.5 82 121.0 83 78.5 84 36.0 85 25.0 86 14.0 87 8.0 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 465543.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.69669313814434 #Duplication Level Percentage of deduplicated Percentage of total 1 75.33498195799541 35.178945350601516 2 9.836940206894736 9.187051565192753 3 3.9269552093942313 5.501274671409593 4 2.2719341457914703 4.243672465443855 5 1.5998535841941477 3.7353935943537238 6 1.225741517970545 3.4342845318812265 7 1.0193671839430407 3.3320753608577753 8 0.8299790185116858 3.100582043083073 9 0.7136590024126311 2.999296389684316 >10 3.163278970930119 21.73181888681873 >50 0.04371651301450853 1.3967587217324657 >100 0.027150255450713468 2.596467020692359 >500 0.0041415643907868 1.2972665235388374 >1k 0.0023008691059926666 2.26511287470977 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4974 1.0684297691083315 No Hit GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC 1697 0.36452057060250076 Illumina Single End Adapter 1 (95% over 21bp) CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCC 1653 0.3550692417241802 No Hit GAATCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTC 1140 0.24487533912012424 No Hit CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT 1077 0.23134275458980158 Illumina Single End Adapter 1 (95% over 22bp) GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGC 872 0.18730815413398977 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCG 828 0.17785682525566918 No Hit TCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC 723 0.15530251770513143 Illumina Single End Adapter 1 (95% over 21bp) CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC 721 0.15487291184702595 Illumina Single End Adapter 1 (95% over 21bp) CGTTTTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCG 704 0.15122126205312936 No Hit CGTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCT 588 0.12630412228301144 No Hit TGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTT 570 0.12243766956006212 Illumina Single End Adapter 1 (95% over 23bp) ACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC 527 0.11320114361079428 Illumina Single End Adapter 1 (95% over 21bp) CGTTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTC 504 0.10826067624258125 No Hit GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 500 0.10740146452637028 No Hit CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGC 484 0.10396461766152643 No Hit CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG 480 0.10310540594531546 No Hit CGTTTTTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGT 473 0.10160178544194629 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.1480292905274058E-4 0.0 0.0 0.25196383577886466 0.0 2 2.1480292905274058E-4 0.0 0.0 1.0950653323108714 0.0 3 2.1480292905274058E-4 0.0 0.0 1.6483976775507312 0.0 4 2.1480292905274058E-4 0.0 0.0 2.348870029191718 0.0 5 2.1480292905274058E-4 0.0 0.0 4.331930670206619 0.0 6 2.1480292905274058E-4 0.0 0.0 5.41131538869664 0.0 7 2.1480292905274058E-4 0.0 0.0 7.40210893515744 0.0 8 2.1480292905274058E-4 0.0 0.0 9.293019119608715 0.0 9 2.1480292905274058E-4 0.0 0.0 11.482720178372352 0.0 10 2.1480292905274058E-4 0.0 0.0 12.982903834876693 0.0 11 2.1480292905274058E-4 0.0 0.0 15.57944164126622 0.0 12 2.1480292905274058E-4 0.0 0.0 17.371327675424183 0.0 13 2.1480292905274058E-4 0.0 0.0 18.03420951448094 0.0 14 2.1480292905274058E-4 0.0 0.0 18.4270840717184 0.0 15 4.2960585810548115E-4 0.0 0.0 19.27963689712873 0.0 16 4.2960585810548115E-4 0.0 0.0 20.612489071900985 0.0 17 4.2960585810548115E-4 0.0 0.0 22.80347894823894 0.0 18 4.2960585810548115E-4 0.0 0.0 23.602760647244185 0.0 19 4.2960585810548115E-4 0.0 0.0 24.705344082071903 0.0 20 4.2960585810548115E-4 0.0 0.0 25.49818169320557 0.0 21 4.2960585810548115E-4 0.0 0.0 26.39713195129129 0.0 22 4.2960585810548115E-4 0.0 0.0 27.347849715278716 0.0 23 4.2960585810548115E-4 0.0 0.0 28.03844113218328 0.0 24 4.2960585810548115E-4 0.0 0.0 28.657288370784222 0.0 25 4.2960585810548115E-4 0.0 0.0 29.25658854284137 0.0 26 4.2960585810548115E-4 0.0 0.0 29.91517432331707 0.0 27 4.2960585810548115E-4 0.0 0.0 30.521133386174853 0.0 28 4.2960585810548115E-4 0.0 0.0 31.12752205489074 0.0 29 4.2960585810548115E-4 0.0 0.0 31.775367688913807 0.0 30 4.2960585810548115E-4 0.0 0.0 32.42085049071729 0.0 31 4.2960585810548115E-4 0.0 0.0 33.0644000661593 0.0 32 4.2960585810548115E-4 0.0 0.0 33.69076540727709 0.0 33 4.2960585810548115E-4 0.0 0.0 34.27674779773297 0.0 34 4.2960585810548115E-4 0.0 0.0 34.894735824617705 0.0 35 4.2960585810548115E-4 0.0 0.0 35.47341491548579 0.0 36 4.2960585810548115E-4 0.0 0.0 36.087536489647576 0.0 37 4.2960585810548115E-4 0.0 0.0 36.69779161108641 0.0 38 4.2960585810548115E-4 0.0 0.0 37.349288894903374 0.0 39 4.2960585810548115E-4 0.0 0.0 37.938493329295035 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTAGCG 50 2.1827873E-11 45.0 1 TCGCATC 20 7.0289575E-4 45.0 34 TTAATCG 35 1.2098462E-7 45.0 19 GGCACGT 20 7.0289575E-4 45.0 7 AACGGTA 20 7.0289575E-4 45.0 4 TAGCACG 20 7.0289575E-4 45.0 1 CGAGGTT 20 7.0289575E-4 45.0 4 ATAAGCG 35 1.2098462E-7 45.0 1 CGACTTA 20 7.0289575E-4 45.0 11 CGGTATT 20 7.0289575E-4 45.0 6 GACTGCG 20 7.0289575E-4 45.0 1 GCGTCAT 20 7.0289575E-4 45.0 36 TACAACG 20 7.0289575E-4 45.0 1 TACCGGA 20 7.0289575E-4 45.0 3 CGTTTTT 3415 0.0 43.155197 1 CGGACTG 70 0.0 41.785713 6 GTACGAG 60 3.6379788E-12 41.249996 1 CATACGA 55 6.002665E-11 40.909092 17 ACGGATG 50 1.0786607E-9 40.5 5 TTGTGCG 45 1.924127E-8 40.0 1 >>END_MODULE