SRR3551668_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551668_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	370619
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	3642	0.9826803266966885	Illumina PCR Primer Index 7 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	3255	0.8782604237775181	RNA PCR Primer, Index 16 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG	3165	0.8539767254242226	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGC	3045	0.8215984609531622	No Hit
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	2814	0.7592703018463706	RNA PCR Primer, Index 16 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	2489	0.6715791689039148	RNA PCR Primer, Index 16 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1500	0.4047283058882572	Illumina PCR Primer Index 7 (95% over 22bp)
TGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCTT	1164	0.3140691653692876	Illumina PCR Primer Index 7 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCC	1150	0.3102917011809972	No Hit
ACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1116	0.30111785958086335	Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1063	0.2868174594394783	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTCCACCT	813	0.21936274179143542	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT	757	0.20425288503827382	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	723	0.19507904343813998	RNA PCR Primer, Index 16 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	641	0.17295389604958192	Illumina PCR Primer Index 7 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	632	0.17052552621425238	Illumina PCR Primer Index 7 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTA	626	0.16890661299069934	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT	535	0.14435309576681174	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTC	531	0.14327382028444305	No Hit
GGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	461	0.12438649934299105	RNA PCR Primer, Index 16 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	438	0.1181806653193711	RNA PCR Primer, Index 16 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	414	0.111705012425159	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG	403	0.1087370048486451	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAC	20	7.0270937E-4	45.000004	4
CATGCCC	20	7.0270937E-4	45.000004	40
AGCCCAT	20	7.0270937E-4	45.000004	11
TTCACGG	40	6.7939254E-9	45.000004	2
CATTACA	20	7.0270937E-4	45.000004	7
ACTAGTA	20	7.0270937E-4	45.000004	21
CTAACGG	20	7.0270937E-4	45.000004	2
AATTGCG	20	7.0270937E-4	45.000004	1
CGAATAT	20	7.0270937E-4	45.000004	14
AGCGAAC	20	7.0270937E-4	45.000004	24
CTCGAAT	20	7.0270937E-4	45.000004	43
AACCTAT	20	7.0270937E-4	45.000004	30
GCAATCG	20	7.0270937E-4	45.000004	45
ATTATCG	20	7.0270937E-4	45.000004	30
TTCGCTT	20	7.0270937E-4	45.000004	3
AAGGCGT	20	7.0270937E-4	45.000004	26
TATCGCG	20	7.0270937E-4	45.000004	32
GCGTAAG	20	7.0270937E-4	45.000004	1
TACCGGA	30	2.161507E-6	45.000004	3
TATCCAG	20	7.0270937E-4	45.000004	12

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content