##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551668_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	370619
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.308572954975325	33.0	31.0	34.0	30.0	34.0
2	32.44557618470721	34.0	31.0	34.0	30.0	34.0
3	32.50076493649813	34.0	31.0	34.0	30.0	34.0
4	35.985281380609194	37.0	35.0	37.0	35.0	37.0
5	35.99616857203759	37.0	35.0	37.0	35.0	37.0
6	36.02805576616417	37.0	35.0	37.0	35.0	37.0
7	36.13493371899444	37.0	35.0	37.0	35.0	37.0
8	35.7841610926585	37.0	35.0	37.0	35.0	37.0
9	37.57225884263894	39.0	37.0	39.0	35.0	39.0
10	37.63928724647145	39.0	37.0	39.0	35.0	39.0
11	37.67061051915849	39.0	37.0	39.0	35.0	39.0
12	37.65499340292861	39.0	37.0	39.0	35.0	39.0
13	37.597055196846355	39.0	37.0	39.0	35.0	39.0
14	38.9263232591961	40.0	38.0	41.0	35.0	41.0
15	38.908221111168075	40.0	38.0	41.0	35.0	41.0
16	38.953717969127325	40.0	38.0	41.0	35.0	41.0
17	38.91822329670092	40.0	38.0	41.0	35.0	41.0
18	38.86610508365734	40.0	38.0	41.0	35.0	41.0
19	38.87854643178034	40.0	38.0	41.0	35.0	41.0
20	38.71291811806734	40.0	38.0	41.0	35.0	41.0
21	38.74214219994118	40.0	38.0	41.0	35.0	41.0
22	38.741616053143524	40.0	38.0	41.0	35.0	41.0
23	38.73730434759146	40.0	38.0	41.0	35.0	41.0
24	38.68467617688246	40.0	38.0	41.0	35.0	41.0
25	38.67231847260934	40.0	38.0	41.0	35.0	41.0
26	38.587662801961045	40.0	38.0	41.0	35.0	41.0
27	38.50159058224214	40.0	38.0	41.0	35.0	41.0
28	38.43123800992394	40.0	38.0	41.0	35.0	41.0
29	38.3574074723638	40.0	38.0	41.0	34.0	41.0
30	38.21989968134391	40.0	38.0	41.0	34.0	41.0
31	38.0993769882278	40.0	37.0	41.0	34.0	41.0
32	37.97652575825848	40.0	37.0	41.0	34.0	41.0
33	37.820079380711725	40.0	37.0	41.0	33.0	41.0
34	37.73342974860976	40.0	37.0	41.0	33.0	41.0
35	37.71768851569941	40.0	37.0	41.0	33.0	41.0
36	37.653566061103184	40.0	37.0	41.0	33.0	41.0
37	37.555144231677275	40.0	37.0	41.0	33.0	41.0
38	37.53883368094998	40.0	37.0	41.0	33.0	41.0
39	37.504901259784305	40.0	37.0	41.0	33.0	41.0
40	37.44459944039566	40.0	37.0	41.0	33.0	41.0
41	37.393970627517746	40.0	36.0	41.0	33.0	41.0
42	37.37144614820071	40.0	36.0	41.0	33.0	41.0
43	37.29367355694122	40.0	36.0	41.0	33.0	41.0
44	37.234183892353066	40.0	36.0	41.0	33.0	41.0
45	37.193362995421175	40.0	36.0	41.0	33.0	41.0
46	37.11274381507694	39.0	35.0	41.0	33.0	41.0
47	37.0188171680351	39.0	35.0	41.0	32.0	41.0
48	37.06524220290919	39.0	35.0	41.0	33.0	41.0
49	37.03589400435488	39.0	35.0	41.0	32.0	41.0
50	36.94779544491783	39.0	35.0	41.0	32.0	41.0
51	36.271583486005845	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	5.0
15	7.0
16	9.0
17	21.0
18	44.0
19	104.0
20	286.0
21	519.0
22	817.0
23	1188.0
24	1766.0
25	2118.0
26	2470.0
27	2675.0
28	2714.0
29	3182.0
30	3677.0
31	4716.0
32	6318.0
33	8693.0
34	15146.0
35	21634.0
36	23726.0
37	36602.0
38	70354.0
39	161508.0
40	319.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.475580043117056	22.918684686969637	23.506080368248796	14.099654901664513
2	31.18944252723147	28.541709950110487	21.47650282365448	18.792344699003557
3	29.691138338833138	28.959659380657765	24.78529163372628	16.563910646782816
4	27.763282508452075	27.069848011030196	26.544510669987236	18.62235881053049
5	29.565672564007784	29.72945261845723	19.99114994104458	20.71372487649041
6	36.763360755924545	28.442686424603163	17.765414077529755	17.028538741942533
7	72.05162174632169	5.355364943513419	14.890224192499574	7.702789117665311
8	62.08289375342333	9.584775740045707	17.29646888044056	11.035861626090405
9	31.89420941721822	24.970927016693693	20.67541059686632	22.45945296922176
10	26.038330468756328	24.50683855927516	30.065107293473893	19.389723678494626
11	23.675526618980676	18.62856464455411	33.792115352963556	23.903793383501657
12	23.163680221467327	18.564077934482583	35.72536756075647	22.546874283293626
13	18.863846699710486	25.27258451401574	34.968525628745425	20.895043157528352
14	16.356150116426843	22.86175290527469	38.45485525566687	22.327241722631598
15	16.016987796092483	24.027640245103463	35.867562105558534	24.087809853245517
16	15.724773959241162	23.171774787585093	29.730262075069007	31.373189178104738
17	18.83740445039245	22.572776894870472	31.3124799322215	27.277338722515577
18	19.396738969130023	25.14684892031979	33.08545973088266	22.37095237966753
19	20.652206173995395	21.88311986163688	29.97552742843729	27.48914653593043
20	18.472609337351837	27.964027748172654	32.66804993807657	20.895312976398944
21	18.498511948928684	26.12494232621641	29.519263718265925	25.857282006588978
22	16.018606709316035	26.71152855088379	29.560815824337123	27.709048915463047
23	16.474600600616807	23.465337718789378	30.62552108769383	29.43454059289999
24	19.845717569795397	24.80040149047944	29.0516676155297	26.302213324195467
25	15.713711385546883	27.54391976666064	28.310205359142408	28.43216348865007
26	17.23926727987502	27.71039800981601	27.71147728529838	27.338857425010595
27	22.21823489891236	24.696251406430864	30.68110377503582	22.404409919620957
28	18.008790698803892	25.29767766898081	28.285112204177338	28.408419428037956
29	19.946090189655685	24.49550616671029	26.612774844247056	28.94562879938697
30	23.83579902811243	26.44818533318583	23.143713625043507	26.572302013658234
31	21.40554046068874	26.97352267422879	23.945075670702256	27.675861194380214
32	21.000272517059297	27.640784741203227	21.056934479883655	30.302008261853818
33	19.277479028328283	21.84426594427161	26.059915978403698	32.81833904899641
34	19.514379996708207	24.77908579970266	25.16924388657894	30.53729031701019
35	20.036479511304062	23.42378561271818	24.632304334100517	31.907430541877236
36	23.063307601607043	25.401827753029394	22.319147156513832	29.215717488849734
37	19.440449626165954	24.436955471791784	28.265145607753517	27.857449294288745
38	18.63315156535418	22.305656212984225	23.179059897091083	35.88213232457051
39	22.483197029833875	21.828886268647857	24.593720235605836	31.094196465912432
40	18.270245184407706	21.94355928864953	25.8289510251768	33.95724450176596
41	20.595544211171042	25.720214020328154	22.367714553220424	31.31652721528038
42	20.971401897905935	18.58377471203581	28.112697945868938	32.332125444189316
43	22.74734970414361	19.75667734249998	28.64586003415907	28.85011291919734
44	19.34547338371751	20.14359760292915	28.415974356414537	32.094954656938796
45	20.786036333809115	20.12578955747007	26.24528154250052	32.84289256622029
46	22.96860117802919	21.304358384216677	28.01853116003227	27.708509277721866
47	18.18471260242999	19.14904524592641	32.0293886713849	30.6368534802587
48	18.60509040281259	19.391072772847586	28.161804980316713	33.842031844023104
49	20.121202636670006	16.623540617183686	34.22895210445228	29.02630464169403
50	20.653285449477764	16.550419703253205	29.282902387627185	33.51339245964184
51	20.01408454504491	16.040462037834004	30.05917127832086	33.88628213880022
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	273.0
1	402.0
2	531.0
3	1253.0
4	1975.0
5	1356.5
6	738.0
7	791.5
8	845.0
9	897.0
10	949.0
11	983.0
12	1017.0
13	1024.5
14	1032.0
15	1065.5
16	1099.0
17	1073.0
18	1047.0
19	1122.0
20	1197.0
21	1145.0
22	1093.0
23	1134.0
24	1175.0
25	1313.5
26	1863.0
27	2274.0
28	2460.5
29	2647.0
30	2780.0
31	2913.0
32	3539.5
33	4166.0
34	4794.0
35	5422.0
36	5606.5
37	5791.0
38	6772.0
39	7753.0
40	8632.5
41	9512.0
42	10601.5
43	11691.0
44	14371.5
45	17052.0
46	21080.5
47	25109.0
48	39140.0
49	53171.0
50	54171.0
51	55171.0
52	52495.5
53	49820.0
54	39245.0
55	28670.0
56	23614.0
57	18558.0
58	16420.0
59	14282.0
60	12876.5
61	11471.0
62	10388.5
63	9306.0
64	8309.0
65	7312.0
66	5966.5
67	4621.0
68	3949.0
69	3277.0
70	2733.5
71	2190.0
72	1948.5
73	1707.0
74	1359.5
75	872.5
76	733.0
77	512.5
78	292.0
79	231.0
80	170.0
81	115.0
82	60.0
83	46.0
84	32.0
85	19.0
86	6.0
87	5.5
88	5.0
89	2.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	370619.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.06737071221944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.55506677292314	26.858458824702613
2	11.955058692586547	9.101953022740673
3	4.703906439417181	5.371960506746701
4	2.4558930344358956	3.7395756228571497
5	1.5881805352667102	3.022892859696449
6	1.2536800864774722	2.863458276387916
7	1.0759344065355851	2.8670595740935356
8	0.9198188062657006	2.8012066788950065
9	0.7600338831892351	2.6039242426691076
>10	4.593490573334275	26.98179181406215
>50	0.07301269013026858	1.936136232332749
>100	0.05245571912271724	3.847716590499327
>500	0.005670888553807269	1.4188438062168076
>1k	0.007797471761484994	6.5850219480998025
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	3642	0.9826803266966885	Illumina PCR Primer Index 7 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	3255	0.8782604237775181	RNA PCR Primer, Index 16 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG	3165	0.8539767254242226	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGC	3045	0.8215984609531622	No Hit
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	2814	0.7592703018463706	RNA PCR Primer, Index 16 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	2489	0.6715791689039148	RNA PCR Primer, Index 16 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1500	0.4047283058882572	Illumina PCR Primer Index 7 (95% over 22bp)
TGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCTT	1164	0.3140691653692876	Illumina PCR Primer Index 7 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCC	1150	0.3102917011809972	No Hit
ACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1116	0.30111785958086335	Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1063	0.2868174594394783	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTCCACCT	813	0.21936274179143542	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT	757	0.20425288503827382	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	723	0.19507904343813998	RNA PCR Primer, Index 16 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	641	0.17295389604958192	Illumina PCR Primer Index 7 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	632	0.17052552621425238	Illumina PCR Primer Index 7 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTA	626	0.16890661299069934	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT	535	0.14435309576681174	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTC	531	0.14327382028444305	No Hit
GGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	461	0.12438649934299105	RNA PCR Primer, Index 16 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	438	0.1181806653193711	RNA PCR Primer, Index 16 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	414	0.111705012425159	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG	403	0.1087370048486451	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7776179850466381	0.0
2	0.0	0.0	0.0	3.201940537317299	0.0
3	0.0	0.0	0.0	4.3767318998756135	0.0
4	0.0	0.0	0.0	5.672941754200405	0.0
5	0.0	0.0	0.0	9.664372306870398	0.0
6	0.0	0.0	0.0	11.126250947738782	0.0
7	0.0	0.0	0.0	14.159554690935975	0.0
8	2.698188705921715E-4	0.0	0.0	16.886074378269868	0.0
9	2.698188705921715E-4	0.0	0.0	19.326046425034875	0.0
10	2.698188705921715E-4	0.0	0.0	22.030980602721392	0.0
11	2.698188705921715E-4	0.0	0.0	25.72237257129289	0.0
12	2.698188705921715E-4	0.0	0.0	28.894902851715642	0.0
13	2.698188705921715E-4	0.0	0.0	29.942609526225045	0.0
14	2.698188705921715E-4	0.0	0.0	30.528656113151243	0.0
15	2.698188705921715E-4	0.0	0.0	31.9052719909125	0.0
16	2.698188705921715E-4	0.0	0.0	33.83771474209363	0.0
17	2.698188705921715E-4	0.0	0.0	37.46057271753472	0.0
18	2.698188705921715E-4	0.0	0.0	38.60271599675138	0.0
19	2.698188705921715E-4	0.0	0.0	40.41023261084834	0.0
20	2.698188705921715E-4	0.0	0.0	41.44660689279287	0.0
21	2.698188705921715E-4	0.0	0.0	42.544769696103	0.0
22	2.698188705921715E-4	0.0	0.0	43.586270536588785	0.0
23	2.698188705921715E-4	0.0	0.0	44.34931830262345	0.0
24	2.698188705921715E-4	0.0	0.0	44.993645765597556	0.0
25	2.698188705921715E-4	0.0	0.0	45.609642247159485	0.0
26	2.698188705921715E-4	0.0	0.0	46.34678740161729	0.0
27	2.698188705921715E-4	0.0	0.0	46.955498773673234	0.0
28	2.698188705921715E-4	0.0	0.0	47.55800431170555	0.0
29	2.698188705921715E-4	0.0	0.0	48.188840831150046	0.0
30	2.698188705921715E-4	0.0	0.0	48.75653973487598	0.0
31	2.698188705921715E-4	0.0	0.0	49.30076439686039	0.0
32	2.698188705921715E-4	0.0	0.0	49.82340354919742	0.0
33	2.698188705921715E-4	0.0	0.0	50.33363103348722	0.0
34	2.698188705921715E-4	0.0	0.0	50.925883454437034	0.0
35	2.698188705921715E-4	0.0	0.0	51.42855601035025	0.0
36	2.698188705921715E-4	0.0	0.0	51.93662494367531	0.0
37	2.698188705921715E-4	0.0	0.0	52.425536737188324	0.0
38	2.698188705921715E-4	0.0	0.0	52.92497146665443	0.0
39	2.698188705921715E-4	0.0	0.0	53.435468769814825	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAC	20	7.0270937E-4	45.000004	4
CATGCCC	20	7.0270937E-4	45.000004	40
AGCCCAT	20	7.0270937E-4	45.000004	11
TTCACGG	40	6.7939254E-9	45.000004	2
CATTACA	20	7.0270937E-4	45.000004	7
ACTAGTA	20	7.0270937E-4	45.000004	21
CTAACGG	20	7.0270937E-4	45.000004	2
AATTGCG	20	7.0270937E-4	45.000004	1
CGAATAT	20	7.0270937E-4	45.000004	14
AGCGAAC	20	7.0270937E-4	45.000004	24
CTCGAAT	20	7.0270937E-4	45.000004	43
AACCTAT	20	7.0270937E-4	45.000004	30
GCAATCG	20	7.0270937E-4	45.000004	45
ATTATCG	20	7.0270937E-4	45.000004	30
TTCGCTT	20	7.0270937E-4	45.000004	3
AAGGCGT	20	7.0270937E-4	45.000004	26
TATCGCG	20	7.0270937E-4	45.000004	32
GCGTAAG	20	7.0270937E-4	45.000004	1
TACCGGA	30	2.161507E-6	45.000004	3
TATCCAG	20	7.0270937E-4	45.000004	12
>>END_MODULE