##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551667_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	650753
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.490056903310474	34.0	31.0	34.0	31.0	34.0
2	32.62677697989867	34.0	31.0	34.0	31.0	34.0
3	32.6446731709266	34.0	31.0	34.0	31.0	34.0
4	36.10915047644805	37.0	37.0	37.0	35.0	37.0
5	36.12273320292031	37.0	35.0	37.0	35.0	37.0
6	36.1534529998325	37.0	36.0	37.0	35.0	37.0
7	36.26343943093616	37.0	37.0	37.0	35.0	37.0
8	35.96337781001394	37.0	37.0	37.0	35.0	37.0
9	37.66212833440645	39.0	37.0	39.0	35.0	39.0
10	37.752314626286775	39.0	37.0	39.0	35.0	39.0
11	37.80128558761927	39.0	38.0	39.0	35.0	39.0
12	37.78880927172061	39.0	38.0	39.0	35.0	39.0
13	37.76176521660292	39.0	38.0	39.0	35.0	39.0
14	39.17418744131798	40.0	39.0	41.0	35.0	41.0
15	39.166613138932895	40.0	39.0	41.0	35.0	41.0
16	39.17040105846611	41.0	39.0	41.0	35.0	41.0
17	39.13054108087093	40.0	39.0	41.0	35.0	41.0
18	39.01710326345019	40.0	39.0	41.0	35.0	41.0
19	38.980253644624	40.0	38.0	41.0	35.0	41.0
20	38.83335766412141	40.0	38.0	41.0	35.0	41.0
21	38.811450734764186	40.0	38.0	41.0	35.0	41.0
22	38.804245235903636	40.0	38.0	41.0	35.0	41.0
23	38.79447040582218	40.0	38.0	41.0	35.0	41.0
24	38.73664047649415	40.0	38.0	41.0	35.0	41.0
25	38.712920263141314	40.0	38.0	41.0	35.0	41.0
26	38.65718790385907	40.0	38.0	41.0	35.0	41.0
27	38.55041313678154	40.0	38.0	41.0	35.0	41.0
28	38.497386873360554	40.0	38.0	41.0	35.0	41.0
29	38.459569145282465	40.0	38.0	41.0	35.0	41.0
30	38.30829054956335	40.0	38.0	41.0	34.0	41.0
31	38.208232616676376	40.0	38.0	41.0	34.0	41.0
32	38.02001066456859	40.0	38.0	41.0	34.0	41.0
33	37.82812219075441	40.0	37.0	41.0	33.0	41.0
34	37.64294747776806	40.0	37.0	41.0	33.0	41.0
35	37.540246452955266	40.0	37.0	41.0	33.0	41.0
36	37.46612923797508	40.0	37.0	41.0	33.0	41.0
37	37.40051601759807	40.0	37.0	41.0	33.0	41.0
38	37.366146602474366	40.0	37.0	41.0	33.0	41.0
39	37.31743380360905	40.0	37.0	41.0	33.0	41.0
40	37.25303456150029	40.0	37.0	41.0	33.0	41.0
41	37.20042473872575	40.0	36.0	41.0	32.0	41.0
42	37.155558253285044	40.0	36.0	41.0	32.0	41.0
43	37.145116503496716	40.0	36.0	41.0	32.0	41.0
44	37.09776520430947	40.0	36.0	41.0	32.0	41.0
45	37.03926989195593	40.0	36.0	41.0	32.0	41.0
46	36.93622157715754	40.0	36.0	41.0	32.0	41.0
47	36.84913169820193	40.0	35.0	41.0	31.0	41.0
48	36.83343910823308	40.0	35.0	41.0	31.0	41.0
49	36.773517755584685	40.0	35.0	41.0	31.0	41.0
50	36.6804870665214	39.0	35.0	41.0	31.0	41.0
51	36.01701567261311	39.0	35.0	41.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	5.0
14	7.0
15	11.0
16	19.0
17	53.0
18	96.0
19	224.0
20	507.0
21	814.0
22	1275.0
23	1900.0
24	3101.0
25	4821.0
26	6332.0
27	6871.0
28	6694.0
29	6319.0
30	6771.0
31	8008.0
32	10077.0
33	13385.0
34	23756.0
35	33534.0
36	38411.0
37	56608.0
38	110224.0
39	310031.0
40	894.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.96908197119337	22.18445400943215	23.18006985753427	14.666394161840207
2	35.720465368580705	26.343174752940058	21.46036975626697	16.475990122212266
3	30.363901511018774	27.088618876901066	26.336874359395964	16.2106052526842
4	27.78089382607533	27.206482336616194	26.700299499195545	18.312324338112926
5	29.182808223703926	30.364516183559658	21.516458625622935	18.93621696711348
6	37.45860564607462	28.331333086439862	19.393533337533594	14.816527929951917
7	75.65696969510705	4.976696227293612	13.553375858428621	5.8129582191707145
8	66.77787117385552	8.837608124741646	16.11594568138756	8.268575020015275
9	31.615835808670877	25.37352881969042	22.646534092044142	20.364101279594564
10	26.239448761665336	26.65020368711324	29.09829074933193	18.012056801889504
11	24.06535198454713	21.366939530052107	33.4779862712888	21.08972221411196
12	23.506768313015844	20.94727185276134	34.94075325046523	20.605206583757585
13	21.300554895636285	25.384900261696835	33.495811774974534	19.81873306769235
14	19.144437290339038	24.902843321506012	36.58730731936694	19.365412068788004
15	20.074590512836668	24.445834287356337	34.3107138960558	21.168861303751193
16	19.980084609675252	24.013412154842158	31.12271476274408	24.883788472738505
17	21.37646695443586	24.166619285658307	31.572194058267883	22.884719701637948
18	21.484341985361574	26.4928475166461	32.1175622701701	19.90524822782223
19	22.98322097631513	23.0858712906433	31.131627514586945	22.79928021845462
20	21.33144219081587	27.249355746343085	31.422367626426617	19.99683443641443
21	19.92491774913062	26.697533472761553	30.427827455271046	22.949721322836776
22	18.757654593985738	26.6435959572987	30.670162104515846	23.92858734419972
23	19.137522224254056	24.334117553050085	31.601852008365693	24.926508214330166
24	20.701249168271218	26.21609120511162	30.170586996909734	22.91207262970743
25	18.934833953896486	26.760076403796834	30.336241246678846	23.968848395627834
26	19.296415076073412	26.05212730482994	30.66970110011018	23.981756518986465
27	21.91814713109275	24.67234111867329	32.543069336599295	20.866442413634665
28	20.42264883911407	25.1106026403259	29.574200964113878	24.89254755644615
29	22.512381809995496	23.342189740193284	30.45763907350408	23.68778937630714
30	22.75425545483463	25.203571862135092	29.50566497580495	22.536507707225322
31	23.68440867733226	24.30492060735794	28.14708499230891	23.863585723000895
32	21.969779624527277	25.981747298898355	29.80209080864783	22.24638226792654
33	22.728900212523033	22.598896970125377	30.929246580499818	23.74295623685177
34	21.218188775157394	22.9365058632077	32.11356689865433	23.73173846298058
35	22.508079102209287	25.12228141860276	30.368972559481094	22.000666919706863
36	23.231548682833576	24.316445717499573	32.15244493686545	20.2995606628014
37	20.96263866628352	24.77560610554235	32.68905406506001	21.572701163114118
38	20.823107999502117	24.039996742235534	31.062169517466685	24.074725740795664
39	23.999274686401755	23.69209208409335	32.19316699269923	20.11546623680567
40	21.28503441397888	22.517145522187374	33.349519710243364	22.84830035359038
41	21.90493167146367	24.910065723861436	30.74115678298832	22.44384582168657
42	19.711165373037083	23.701004835936214	33.593852045246045	22.993977745780658
43	22.789752794070868	22.226405410347706	32.04211121577619	22.94173057980524
44	21.163790255288873	22.208579906661974	32.868692115134316	23.758937722914837
45	23.10185277670637	22.495939319526766	29.450344447125097	24.951863456641767
46	22.352874285635256	22.64807077339636	32.94706286409744	22.05199207687095
47	20.26452432797083	23.55471277120505	33.7224722744267	22.45829062639742
48	19.86775320282811	21.94135101951124	33.39331512878158	24.797580648879066
49	21.16271457834232	20.706934889274425	34.42827001950049	23.702080512882766
50	21.17193466645563	20.617961038980994	31.72693787043625	26.483166424127123
51	20.413582419135988	20.086269291113528	32.59470183003382	26.905446459716664
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	311.0
1	572.5
2	834.0
3	5181.0
4	9528.0
5	6194.0
6	2860.0
7	2834.5
8	2809.0
9	2846.0
10	2883.0
11	2861.0
12	2839.0
13	2737.0
14	2635.0
15	2595.0
16	2555.0
17	2410.5
18	2266.0
19	2349.5
20	2433.0
21	2470.0
22	2507.0
23	2910.5
24	3314.0
25	3788.5
26	5226.5
27	6190.0
28	7423.5
29	8657.0
30	9324.0
31	9991.0
32	11022.5
33	12054.0
34	13607.5
35	15161.0
36	15883.5
37	16606.0
38	18976.5
39	21347.0
40	25468.0
41	29589.0
42	35001.5
43	40414.0
44	55292.0
45	70170.0
46	72390.0
47	74610.0
48	71863.0
49	69116.0
50	58976.0
51	48836.0
52	43296.5
53	37757.0
54	34090.5
55	30424.0
56	27705.5
57	24987.0
58	23803.0
59	22619.0
60	21197.5
61	19776.0
62	18021.0
63	16266.0
64	13941.5
65	11617.0
66	9707.5
67	7798.0
68	6528.0
69	5258.0
70	4370.0
71	3482.0
72	2913.0
73	2344.0
74	2012.5
75	1325.5
76	970.0
77	727.0
78	484.0
79	367.5
80	251.0
81	216.5
82	182.0
83	114.0
84	46.0
85	31.5
86	17.0
87	10.5
88	4.0
89	8.0
90	12.0
91	6.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	650753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.908680534652945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.44341391592462	29.635644767261915
2	10.33975805864845	8.459717184537054
3	3.998803729123971	4.907573528265342
4	2.449345278714342	4.0079793410394196
5	1.7944163601247498	3.670360281124907
6	1.412918207010446	3.4680371731310977
7	1.1988747088162908	3.4331067724828497
8	1.0243831524760665	3.352493050377926
9	0.8688863409969154	3.1990494370289695
>10	4.373862244668994	26.654434688537233
>50	0.056147678579493626	1.5762471805766978
>100	0.029392743686734855	2.2870613860865
>500	0.006029280756253291	1.5737806477862666
>1k	0.003391470425392476	2.8771713204312745
>5k	3.7683004726583066E-4	0.8973432413325358
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5821	0.8945022151261692	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC	2788	0.4284267609984126	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCG	2755	0.4233557125360928	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	2411	0.3704938740197894	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGC	2361	0.3628104672586988	No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	2188	0.33622587986532526	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	1943	0.2985771867359812	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCC	1943	0.2985771867359812	No Hit
TCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	1145	0.17595001482897504	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG	1130	0.17364499280064788	No Hit
TGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTT	790	0.12139782682523169	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT	771	0.11847813225601726	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCG	768	0.11801712785035183	No Hit
ACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	744	0.11432909260502833	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCTGTATT	738	0.11340708379369746	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCT	714	0.10971904854837397	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCT	694	0.10664568584393772	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5366813522181227E-4	0.0	0.0	0.34390928662641584	0.0
2	1.5366813522181227E-4	0.0	0.0	1.326770679505127	0.0
3	1.5366813522181227E-4	0.0	0.0	1.9927683775564615	0.0
4	1.5366813522181227E-4	0.0	0.0	2.788154645464562	0.0
5	1.5366813522181227E-4	0.0	0.0	4.998670770630332	0.0
6	1.5366813522181227E-4	0.0	0.0	6.129053573321983	0.0
7	1.5366813522181227E-4	0.0	0.0	8.09185666451019	0.0
8	1.5366813522181227E-4	0.0	0.0	9.864725940564238	0.0
9	1.5366813522181227E-4	0.0	0.0	11.704133519169332	0.0
10	1.5366813522181227E-4	0.0	0.0	13.390794971363944	0.0
11	1.5366813522181227E-4	0.0	0.0	15.817983167192468	0.0
12	1.5366813522181227E-4	0.0	0.0	17.758965383179177	0.0
13	1.5366813522181227E-4	0.0	0.0	18.428190112070173	0.0
14	1.5366813522181227E-4	0.0	0.0	18.85123848833582	0.0
15	1.5366813522181227E-4	0.0	0.0	19.759724503767174	0.0
16	1.5366813522181227E-4	0.0	0.0	21.095254266979946	0.0
17	1.5366813522181227E-4	0.0	0.0	23.254906239387296	0.0
18	1.5366813522181227E-4	0.0	0.0	24.096700284132382	0.0
19	1.5366813522181227E-4	0.0	0.0	25.275027545013238	0.0
20	1.5366813522181227E-4	0.0	0.0	26.116821589758327	0.0
21	1.5366813522181227E-4	0.0	0.0	27.034220357032545	0.0
22	1.5366813522181227E-4	0.0	0.0	27.956536504633863	0.0
23	1.5366813522181227E-4	0.0	0.0	28.65757053751577	0.0
24	1.5366813522181227E-4	0.0	0.0	29.27408709602568	0.0
25	1.5366813522181227E-4	0.0	0.0	29.85648932851635	0.0
26	1.5366813522181227E-4	0.0	0.0	30.51956733199847	0.0
27	1.5366813522181227E-4	0.0	0.0	31.134086204750496	0.0
28	1.5366813522181227E-4	0.0	0.0	31.746914728015085	0.0
29	1.5366813522181227E-4	0.0	0.0	32.399235962031675	0.0
30	1.5366813522181227E-4	0.0	0.0	33.000385707019404	0.0
31	1.5366813522181227E-4	0.0	0.0	33.61920728755764	0.0
32	1.5366813522181227E-4	0.0	0.0	34.194233449557665	0.0
33	1.5366813522181227E-4	0.0	0.0	34.76403489496015	0.0
34	1.5366813522181227E-4	0.0	0.0	35.36211127724344	0.0
35	1.5366813522181227E-4	0.0	0.0	35.900410754925446	0.0
36	1.5366813522181227E-4	0.0	0.0	36.466370496947384	0.0
37	1.5366813522181227E-4	0.0	0.0	37.01327539020181	0.0
38	1.5366813522181227E-4	0.0	0.0	37.60459037453535	0.0
39	1.5366813522181227E-4	0.0	0.0	38.179462868400144	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAAGCG	25	3.8889328E-5	45.000004	1
ACGGAGT	30	2.1640444E-6	45.000004	5
CTATGCG	20	7.031028E-4	45.0	1
ATATCCG	20	7.031028E-4	45.0	1
TGCGACG	20	7.031028E-4	45.0	1
CGTTTTT	3120	0.0	42.764423	1
TAGGCAC	65	0.0	41.53846	5
ACATACG	110	0.0	40.909092	16
AACGGAT	50	1.0804797E-9	40.500004	4
TCGTTGA	90	0.0	40.0	23
TACGAAT	90	0.0	40.0	12
CGAATAT	90	0.0	40.0	14
GCGATTA	85	0.0	39.705883	8
ACGGACA	40	3.455134E-7	39.375	5
TTGCGAG	40	3.455134E-7	39.375	1
GCGATAT	40	3.455134E-7	39.375	8
TTACGGA	40	3.455134E-7	39.375	3
ATTTGCG	40	3.455134E-7	39.375	1
CATACGA	115	0.0	39.130436	17
TGATCCG	35	6.2441104E-6	38.57143	4
>>END_MODULE