FastQCFastQC Report
Sat 18 Jun 2016
SRR3551666_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551666_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542479
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC50990.9399442190388937TruSeq Adapter, Index 27 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT30800.5677639134418107TruSeq Adapter, Index 27 (100% over 25bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC30610.5642614737160333TruSeq Adapter, Index 27 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC24430.4503400131617998TruSeq Adapter, Index 27 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG20510.3780791514510239No Hit
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC18270.33678723047343767TruSeq Adapter, Index 27 (96% over 25bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC16510.3043435782767628No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15000.2765083994034792No Hit
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC14900.2746650100741227TruSeq Adapter, Index 27 (100% over 24bp)
TGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTT14090.25973355650633484TruSeq Adapter, Index 27 (96% over 27bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC11930.2199163469922338No Hit
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG9480.17475330842299885TruSeq Adapter, Index 27 (100% over 23bp)
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG8730.1609278884528249TruSeq Adapter, Index 27 (100% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG8310.1531856532695275TruSeq Adapter, Index 27 (95% over 24bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6930.1277468805244074No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG6710.12369142399982302TruSeq Adapter, Index 27 (100% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT6320.11650220561533259No Hit
AGCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT6310.11631786668239692TruSeq Adapter, Index 27 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA6310.11631786668239692No Hit
GGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG5650.10415149710864384TruSeq Adapter, Index 27 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT5620.10359848030983689TruSeq Adapter, Index 27 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAT302.1633732E-645.0000044
CGGCACA302.1633732E-645.0000046
ACCTGGT253.8880735E-545.010
CACTGCG207.029989E-445.01
TGCGAAG207.029989E-445.01
ACGGAGT253.8880735E-545.05
GTACTAG207.029989E-445.01
AACGATT207.029989E-445.024
GACGGTA207.029989E-445.04
GGACGTA253.8880735E-545.07
ACGTAAG207.029989E-445.01
CCCGGTC207.029989E-445.09
ATCATCG207.029989E-445.011
CTTACGG207.029989E-445.02
ACGGTAT253.8880735E-545.05
CAACCGG1450.041.8965532
GCAACCG1350.041.6666641
TAGGCGA650.041.538465
CGAATAT1200.041.25000414
AACCGGA1550.040.645163
Produced by FastQC (version 0.11.5)