SRR3551665_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551665_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	341081
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2494	0.7312046112213815	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	2342	0.6866404167924921	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	2267	0.6646515050677112	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1886	0.5529478335058241	No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	1884	0.5523614625264966	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG	1818	0.5330112202086895	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC	1772	0.5195246876841572	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC	1414	0.41456428238453624	No Hit
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	863	0.25301907757981246	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	781	0.2289778674273853	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTTGTGGT	755	0.2213550446961279	No Hit
ACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	750	0.21988911724780918	No Hit
TGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTT	696	0.2040571008059669	Illumina Single End Adapter 2 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT	603	0.1767908502672386	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG	553	0.1621315757840513	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCT	512	0.15011097070783772	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	481	0.14102222052826163	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC	425	0.12460383310709186	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTA	422	0.12372427663810064	No Hit
ACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	400	0.11727419586549821	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTC	398	0.11668782488617074	No Hit
GCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	389	0.11404915547919701	No Hit
AGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	382	0.11199685705155081	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	351	0.1029081068719747	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTC	347	0.10173536491331972	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	20	7.0263015E-4	45.000004	1
TACCCAA	20	7.0263015E-4	45.000004	23
ACGGCAC	20	7.0263015E-4	45.000004	5
CGCTATA	20	7.0263015E-4	45.000004	18
TGCGAAG	20	7.0263015E-4	45.000004	1
GTACTCG	20	7.0263015E-4	45.000004	15
CGATGTT	20	7.0263015E-4	45.000004	9
CGATATA	20	7.0263015E-4	45.000004	26
GGCCTAT	55	1.8189894E-12	45.000004	7
CACGGCA	25	3.8850172E-5	45.0	4
GCGCGAC	25	3.8850172E-5	45.0	8
CGATTAG	25	3.8850172E-5	45.0	9
GCCGATA	25	3.8850172E-5	45.0	8
CGGTGTA	25	3.8850172E-5	45.0	6
CGAATAT	75	0.0	45.0	14
GCGATGT	45	3.8380676E-10	45.0	8
AAGGCTC	35	1.208573E-7	45.0	5
TTAATCG	60	0.0	44.999996	19
CGTTGAT	60	0.0	44.999996	24
GTTCAGG	30	2.1609976E-6	44.999996	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content