##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551665_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	341081
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36693629958866	33.0	31.0	34.0	30.0	34.0
2	32.50450186319379	34.0	31.0	34.0	30.0	34.0
3	32.5370982259346	34.0	31.0	34.0	30.0	34.0
4	36.029292162272306	37.0	35.0	37.0	35.0	37.0
5	36.02993423849467	37.0	35.0	37.0	35.0	37.0
6	36.06311404035991	37.0	35.0	37.0	35.0	37.0
7	36.192244657427416	37.0	36.0	37.0	35.0	37.0
8	35.86830694175284	37.0	35.0	37.0	35.0	37.0
9	37.57916154813666	39.0	37.0	39.0	35.0	39.0
10	37.68128978160613	39.0	37.0	39.0	35.0	39.0
11	37.73980374163322	39.0	37.0	39.0	35.0	39.0
12	37.700382020693034	39.0	37.0	39.0	35.0	39.0
13	37.646907919233264	39.0	37.0	39.0	35.0	39.0
14	39.03044438124668	40.0	38.0	41.0	35.0	41.0
15	39.01481466279271	40.0	38.0	41.0	35.0	41.0
16	39.02037345967673	40.0	38.0	41.0	35.0	41.0
17	38.98089896534841	40.0	38.0	41.0	35.0	41.0
18	38.868400761109534	40.0	38.0	41.0	35.0	41.0
19	38.834616997135576	40.0	38.0	41.0	35.0	41.0
20	38.65598201013835	40.0	38.0	41.0	35.0	41.0
21	38.67228605521856	40.0	38.0	41.0	35.0	41.0
22	38.653853483483395	40.0	38.0	41.0	35.0	41.0
23	38.64968438582038	40.0	38.0	41.0	35.0	41.0
24	38.585764085363884	40.0	38.0	41.0	35.0	41.0
25	38.564593161155265	40.0	38.0	41.0	35.0	41.0
26	38.45832221671685	40.0	38.0	41.0	35.0	41.0
27	38.33653589616543	40.0	38.0	41.0	35.0	41.0
28	38.27197645134147	40.0	37.0	41.0	34.0	41.0
29	38.197439904304254	40.0	37.0	41.0	34.0	41.0
30	38.08458987747778	40.0	37.0	41.0	34.0	41.0
31	37.884484917072484	40.0	37.0	41.0	34.0	41.0
32	37.657113706128456	40.0	37.0	41.0	33.0	41.0
33	37.448954940322096	40.0	37.0	41.0	33.0	41.0
34	37.21994189063595	40.0	37.0	41.0	32.0	41.0
35	37.11451238855287	40.0	37.0	41.0	32.0	41.0
36	37.03759224348469	40.0	36.0	41.0	31.0	41.0
37	36.90258912105922	40.0	36.0	41.0	31.0	41.0
38	36.86444862070886	40.0	36.0	41.0	31.0	41.0
39	36.80296175981658	40.0	36.0	41.0	31.0	41.0
40	36.75049914829615	40.0	36.0	41.0	31.0	41.0
41	36.6942221935552	39.0	35.0	41.0	31.0	41.0
42	36.655460139966756	40.0	35.0	41.0	31.0	41.0
43	36.583471374834716	39.0	35.0	41.0	31.0	41.0
44	36.56257018127659	39.0	35.0	41.0	31.0	41.0
45	36.54460670632489	39.0	35.0	41.0	30.0	41.0
46	36.47696588200457	39.0	35.0	41.0	30.0	41.0
47	36.38741823789657	39.0	35.0	41.0	30.0	41.0
48	36.36337116403435	39.0	35.0	41.0	30.0	41.0
49	36.27752352080591	39.0	35.0	41.0	30.0	41.0
50	36.17696676156104	39.0	35.0	41.0	30.0	41.0
51	35.461424119197495	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	1.0
14	2.0
15	5.0
16	15.0
17	18.0
18	63.0
19	152.0
20	354.0
21	628.0
22	1061.0
23	1423.0
24	2151.0
25	3149.0
26	3776.0
27	3842.0
28	3728.0
29	3579.0
30	3768.0
31	4605.0
32	5793.0
33	7987.0
34	13535.0
35	18531.0
36	21103.0
37	31958.0
38	65282.0
39	144228.0
40	341.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.527854087445505	22.537168590452122	22.487327057209285	15.44765026489309
2	33.55126788065005	27.180640375746524	22.879316056889714	16.388775686713714
3	29.024483920241817	27.78841389581947	27.294396345736054	15.892705838202655
4	27.160410576959727	26.714182261691505	28.18773253274149	17.93767462860728
5	28.890498151465486	29.098659849126747	22.51517967872734	19.495662320680424
6	34.957092303587714	28.346932253628903	20.654331375831546	16.04164406695184
7	73.8924185164228	4.419771256680964	15.191406146926974	6.496404079969274
8	64.85878720890345	8.636658154514617	17.417856755433462	9.086697881148465
9	30.009000794532675	25.29487130622931	23.088357311019962	21.60777058821805
10	25.37285864647987	26.700695729166974	30.22185346002856	17.704592164324602
11	23.91543357736139	20.15269100301688	33.70724256115116	22.22463285847057
12	23.112398521172388	20.954553317247225	35.05765492654238	20.87539323503801
13	19.831652891835077	26.64909508298615	33.4691759435442	20.05007608163457
14	17.654457445592104	25.2189362644064	37.544161064380596	19.582445225620894
15	18.212096246932546	25.455536954565044	34.12444551294268	22.207921285559735
16	17.497896394111663	24.780037586379773	29.90345401825373	27.818612001254834
17	20.537350365455712	25.00666996988985	30.68391379173862	23.772065872915817
18	20.663713311500786	27.11731230997915	31.486362476948294	20.73261190157177
19	20.884481985217587	23.125298682717595	30.55725766020388	25.432961671860937
20	19.790313737792488	28.19740765390039	32.0879791017383	19.924299506568822
21	18.43374447711834	28.325822898373115	29.73956332953169	23.50086929497685
22	17.258657034546047	27.406100017297945	30.24706741213964	25.088175536016372
23	18.06022616328673	24.377787094561114	30.91259847367634	26.649388268475814
24	19.934560998707052	27.01440420310718	29.32265356322985	23.72838123495592
25	17.172753686074568	28.156654870837134	29.142344487086646	25.528246956001656
26	17.60960006567355	27.691955869720097	29.12856476907245	25.569879295533905
27	22.291772335603564	25.598025102541627	31.63295522178016	20.477247340074644
28	19.04826126345355	26.642351816723885	29.571568043954365	24.737818875868196
29	20.266153787516746	25.53968119009854	30.997622265678825	23.196542756705885
30	24.56923721931154	26.693659277415044	27.313746588053867	21.423356915219554
31	23.290948484377612	25.853389664038744	28.739214438798992	22.116447412784645
32	23.9274541824376	27.582011311096196	27.996282407991064	20.494252098475144
33	25.191963199357335	23.071059367129802	29.854492041479887	21.882485392032976
34	22.543911856714388	23.3355126788065	32.21727390268001	21.903301561799104
35	26.170616363854922	24.767430610324233	28.799610649669727	20.262342376151118
36	28.39882608529939	25.093746060319983	28.407035279009975	18.100392575370662
37	25.137723883769546	23.404118083387818	32.75233742131635	18.705820611526295
38	25.117494084982745	22.389403103661593	29.991116479663187	22.50198633169247
39	28.26396076005406	22.608705849930075	30.556964474714217	18.570368915301643
40	25.09667791521662	21.635623209736103	30.487479513663914	22.780219361383367
41	26.43272419161431	24.510600121378793	27.073334486529593	21.983341200477305
42	25.947502206220808	21.620084378783925	30.338248099425062	22.0941653155702
43	25.943397609365515	22.40846016048974	30.63465862947511	21.013483600669637
44	22.187691486772938	21.92880869939985	32.152773094953986	23.73072671887323
45	24.623769720389	21.559688167913194	28.794919681835108	25.0216224298627
46	25.257636749042017	21.271486831573732	32.32604571934526	21.144830700038995
47	24.223278341508323	21.091764126409856	33.18947698640498	21.495480545676834
48	23.072818480067784	20.8495929119476	30.999967749596134	25.077620858388478
49	23.113864448620706	18.861795292027406	34.537837053368555	23.48650320598333
50	23.835980309662514	19.428229658057763	30.2974953163618	26.43829471591792
51	21.919719949220273	18.17896628660054	31.460268968368215	28.441044795810967
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	262.0
1	425.5
2	589.0
3	2374.0
4	4159.0
5	2796.5
6	1434.0
7	1433.0
8	1432.0
9	1492.0
10	1552.0
11	1582.5
12	1613.0
13	1543.0
14	1473.0
15	1503.0
16	1533.0
17	1507.0
18	1481.0
19	1479.5
20	1478.0
21	1521.5
22	1565.0
23	1675.0
24	1785.0
25	1962.0
26	2535.5
27	2932.0
28	3333.5
29	3735.0
30	3916.5
31	4098.0
32	4700.5
33	5303.0
34	6044.5
35	6786.0
36	7324.0
37	7862.0
38	8539.5
39	9217.0
40	10445.0
41	11673.0
42	14099.0
43	16525.0
44	19718.0
45	22911.0
46	32688.0
47	42465.0
48	44145.0
49	45825.0
50	43825.0
51	41825.0
52	33352.0
53	24879.0
54	21067.5
55	17256.0
56	15099.5
57	12943.0
58	11657.5
59	10372.0
60	9627.0
61	8882.0
62	7991.5
63	7101.0
64	6153.0
65	5205.0
66	4444.5
67	3684.0
68	3172.0
69	2660.0
70	2153.5
71	1647.0
72	1370.0
73	1093.0
74	964.5
75	631.5
76	427.0
77	350.0
78	273.0
79	189.5
80	106.0
81	77.5
82	49.0
83	28.0
84	7.0
85	7.5
86	8.0
87	4.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	341081.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.84355329191687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.45200154011866	35.81283408418258
2	9.66213760257985	9.052177154005664
3	3.7315765207507767	5.244009108379643
4	2.160807549485564	4.048796143916132
5	1.568361614402162	3.6733815432622228
6	1.1935965205792844	3.3547381332481354
7	0.9367369865228143	3.0716062264083748
8	0.8040880315745242	3.0133072446763007
9	0.6690802200932328	2.8207885451854335
>10	2.7338985701356235	18.8097035729146
>50	0.04135128348567911	1.3729512181244505
>100	0.03633900669953619	3.447933071938017
>500	0.005012276786142923	1.618016260265091
>1k	0.005012276786142923	4.659757693493352
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2494	0.7312046112213815	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	2342	0.6866404167924921	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	2267	0.6646515050677112	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1886	0.5529478335058241	No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	1884	0.5523614625264966	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG	1818	0.5330112202086895	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC	1772	0.5195246876841572	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC	1414	0.41456428238453624	No Hit
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	863	0.25301907757981246	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	781	0.2289778674273853	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTTGTGGT	755	0.2213550446961279	No Hit
ACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	750	0.21988911724780918	No Hit
TGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTT	696	0.2040571008059669	Illumina Single End Adapter 2 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT	603	0.1767908502672386	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG	553	0.1621315757840513	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCT	512	0.15011097070783772	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	481	0.14102222052826163	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC	425	0.12460383310709186	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTA	422	0.12372427663810064	No Hit
ACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	400	0.11727419586549821	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTC	398	0.11668782488617074	No Hit
GCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	389	0.11404915547919701	No Hit
AGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	382	0.11199685705155081	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	351	0.1029081068719747	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTC	347	0.10173536491331972	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5632093256440552	0.0
2	0.0	0.0	0.0	2.31616536834359	0.0
3	0.0	0.0	0.0	3.324430267297211	0.0
4	0.0	0.0	0.0	4.465215007578845	0.0
5	0.0	0.0	0.0	7.618131763422765	0.0
6	0.0	0.0	0.0	9.003726387573627	0.0
7	0.0	0.0	0.0	11.51046232419865	0.0
8	0.0	0.0	0.0	13.636643495240133	0.0
9	0.0	0.0	0.0	15.805336562282859	0.0
10	0.0	0.0	0.0	17.927119950979385	0.0
11	0.0	0.0	0.0	20.888879767562543	0.0
12	2.9318548966374554E-4	0.0	0.0	23.420536470808987	0.0
13	2.9318548966374554E-4	0.0	0.0	24.15643204986499	0.0
14	2.9318548966374554E-4	0.0	0.0	24.624942462347654	0.0
15	2.9318548966374554E-4	0.0	0.0	25.672494216916217	0.0
16	2.9318548966374554E-4	0.0	0.0	27.24602073994154	0.0
17	2.9318548966374554E-4	0.0	0.0	29.75422260401488	0.0
18	2.9318548966374554E-4	0.0	0.0	30.65019746042729	0.0
19	2.9318548966374554E-4	0.0	0.0	32.15951636121625	0.0
20	2.9318548966374554E-4	0.0	0.0	33.10064178303687	0.0
21	2.9318548966374554E-4	0.0	0.0	34.10656119807319	0.0
22	2.9318548966374554E-4	0.0	0.0	35.111014685661175	0.0
23	5.863709793274911E-4	0.0	0.0	35.82638728044072	0.0
24	5.863709793274911E-4	0.0	0.0	36.487813745122125	0.0
25	5.863709793274911E-4	0.0	0.0	37.085618958546505	0.0
26	5.863709793274911E-4	0.0	0.0	37.91328159586726	0.0
27	5.863709793274911E-4	0.0	0.0	38.55565100372053	0.0
28	5.863709793274911E-4	0.0	0.0	39.17397920142136	0.0
29	5.863709793274911E-4	0.0	0.0	39.82045320612992	0.0
30	8.795564689912367E-4	0.0	0.0	40.40506507251943	0.0
31	8.795564689912367E-4	0.0	0.0	40.98645189852264	0.0
32	8.795564689912367E-4	0.0	0.0	41.55142033710467	0.0
33	8.795564689912367E-4	0.0	0.0	42.07534280713379	0.0
34	8.795564689912367E-4	0.0	0.0	42.63767257630885	0.0
35	8.795564689912367E-4	0.0	0.0	43.124653674640335	0.0
36	8.795564689912367E-4	0.0	0.0	43.655905781911045	0.0
37	8.795564689912367E-4	0.0	0.0	44.176016840574526	0.0
38	8.795564689912367E-4	0.0	0.0	44.72720556114237	0.0
39	8.795564689912367E-4	0.0	0.0	45.27253057191693	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	20	7.0263015E-4	45.000004	1
TACCCAA	20	7.0263015E-4	45.000004	23
ACGGCAC	20	7.0263015E-4	45.000004	5
CGCTATA	20	7.0263015E-4	45.000004	18
TGCGAAG	20	7.0263015E-4	45.000004	1
GTACTCG	20	7.0263015E-4	45.000004	15
CGATGTT	20	7.0263015E-4	45.000004	9
CGATATA	20	7.0263015E-4	45.000004	26
GGCCTAT	55	1.8189894E-12	45.000004	7
CACGGCA	25	3.8850172E-5	45.0	4
GCGCGAC	25	3.8850172E-5	45.0	8
CGATTAG	25	3.8850172E-5	45.0	9
GCCGATA	25	3.8850172E-5	45.0	8
CGGTGTA	25	3.8850172E-5	45.0	6
CGAATAT	75	0.0	45.0	14
GCGATGT	45	3.8380676E-10	45.0	8
AAGGCTC	35	1.208573E-7	45.0	5
TTAATCG	60	0.0	44.999996	19
CGTTGAT	60	0.0	44.999996	24
GTTCAGG	30	2.1609976E-6	44.999996	2
>>END_MODULE