SRR3551664_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551664_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	468558
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3543	0.7561497189248716	No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	2333	0.497910610852872	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTC	2285	0.48766641483018114	No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	1853	0.39546865062596304	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCC	1791	0.38223656409665396	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCG	1735	0.3702850020701813	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGC	1609	0.34339398751061767	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	1346	0.2872643301362905	No Hit
TCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	1141	0.24351307628938146	No Hit
ACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	849	0.18119421715134518	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	755	0.16113266660690884	No Hit
TGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCTT	737	0.15729109309839975	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCG	731	0.15601056859556342	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	590	0.1259182427789089	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTC	567	0.12100956551803618	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	539	0.11503378450479984	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT	537	0.11460694300385438	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT	524	0.11183247324770894	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGC	513	0.10948484499250895	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	491	0.10478958848210893	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	477	0.10180169797549077	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGG	25	3.887256E-5	45.0	2
TTAGGCA	20	7.029004E-4	45.0	4
ACCGGAC	20	7.029004E-4	45.0	4
GCAAGCG	35	1.2098644E-7	45.0	1
CGTATGG	20	7.029004E-4	45.0	2
TACTTAG	20	7.029004E-4	45.0	15
CACGGCA	20	7.029004E-4	45.0	4
CGGCATG	20	7.029004E-4	45.0	6
AAAAGCG	20	7.029004E-4	45.0	1
GCACGAG	20	7.029004E-4	45.0	1
TCGATGG	20	7.029004E-4	45.0	2
CGTTAGG	20	7.029004E-4	45.0	2
AACGGAC	25	3.887256E-5	45.0	4
TACGAAT	40	6.7993824E-9	45.0	12
ACGTGGT	20	7.029004E-4	45.0	23
GCTACGA	40	6.7993824E-9	45.0	10
TAAGACG	25	3.887256E-5	45.0	1
CGGAGAT	20	7.029004E-4	45.0	6
TACCGGA	35	1.2098644E-7	45.0	3
TAATCCG	20	7.029004E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content