##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551664_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	468558
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35947097264373	33.0	31.0	34.0	30.0	34.0
2	32.50714959514084	34.0	31.0	34.0	30.0	34.0
3	32.533938167740175	34.0	31.0	34.0	30.0	34.0
4	36.02968255797575	37.0	35.0	37.0	35.0	37.0
5	36.04148045706188	37.0	35.0	37.0	35.0	37.0
6	36.08783544406455	37.0	35.0	37.0	35.0	37.0
7	36.223500612517554	37.0	37.0	37.0	35.0	37.0
8	35.92904613729784	37.0	36.0	37.0	35.0	37.0
9	37.558255328049036	39.0	37.0	39.0	35.0	39.0
10	37.67835785537756	39.0	37.0	39.0	35.0	39.0
11	37.73461983361718	39.0	37.0	39.0	35.0	39.0
12	37.7283538003833	39.0	37.0	39.0	35.0	39.0
13	37.70068380008451	39.0	37.0	39.0	35.0	39.0
14	39.04136947827163	40.0	38.0	41.0	35.0	41.0
15	39.0453284331929	40.0	38.0	41.0	35.0	41.0
16	39.054977185321775	40.0	38.0	41.0	35.0	41.0
17	39.01448059791957	40.0	38.0	41.0	35.0	41.0
18	38.91867388882486	40.0	38.0	41.0	35.0	41.0
19	38.88326311790643	40.0	38.0	41.0	35.0	41.0
20	38.70047891616406	40.0	38.0	41.0	35.0	41.0
21	38.70679403617055	40.0	38.0	41.0	35.0	41.0
22	38.69468027437372	40.0	38.0	41.0	35.0	41.0
23	38.70940630615633	40.0	38.0	41.0	35.0	41.0
24	38.65463827317002	40.0	38.0	41.0	35.0	41.0
25	38.64214889085236	40.0	38.0	41.0	35.0	41.0
26	38.60231177356912	40.0	38.0	41.0	35.0	41.0
27	38.530371906999775	40.0	38.0	41.0	35.0	41.0
28	38.45942658112763	40.0	38.0	41.0	35.0	41.0
29	38.423501466200555	40.0	38.0	41.0	35.0	41.0
30	38.28344409870283	40.0	38.0	41.0	34.0	41.0
31	38.1680816462423	40.0	38.0	41.0	34.0	41.0
32	37.96013086960419	40.0	38.0	41.0	34.0	41.0
33	37.675427588473575	40.0	37.0	41.0	33.0	41.0
34	37.524882725297616	40.0	37.0	41.0	33.0	41.0
35	37.412132542822874	40.0	37.0	41.0	33.0	41.0
36	37.258567776027725	40.0	37.0	41.0	32.0	41.0
37	37.20782912681034	40.0	37.0	41.0	32.0	41.0
38	37.10626646007538	40.0	37.0	41.0	32.0	41.0
39	37.01944476457557	40.0	36.0	41.0	31.0	41.0
40	36.951506537077584	40.0	36.0	41.0	31.0	41.0
41	36.95787074385668	40.0	36.0	41.0	31.0	41.0
42	36.85584495409319	40.0	36.0	41.0	31.0	41.0
43	36.81368795325232	40.0	36.0	41.0	31.0	41.0
44	36.79739754736874	40.0	36.0	41.0	31.0	41.0
45	36.758751317873134	40.0	36.0	41.0	31.0	41.0
46	36.67489190238989	40.0	35.0	41.0	31.0	41.0
47	36.52619526291302	39.0	35.0	41.0	31.0	41.0
48	36.43292826074894	39.0	35.0	41.0	31.0	41.0
49	36.40146577371425	39.0	35.0	41.0	31.0	41.0
50	36.26041813393432	39.0	35.0	41.0	30.0	41.0
51	35.564376235172595	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	2.0
12	1.0
13	3.0
14	1.0
15	5.0
16	15.0
17	28.0
18	83.0
19	170.0
20	374.0
21	705.0
22	1098.0
23	1558.0
24	2612.0
25	3779.0
26	5022.0
27	5192.0
28	4873.0
29	4734.0
30	5108.0
31	6175.0
32	7677.0
33	10297.0
34	17699.0
35	24346.0
36	28509.0
37	44250.0
38	90341.0
39	203421.0
40	478.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.27231634077318	23.47116045398862	24.46292668143538	13.793596523802817
2	34.526995590727296	27.034219883130795	22.495827624328257	15.942956901813648
3	29.285381959117117	27.208798057017486	27.752594129221997	15.753225854643397
4	27.07797113697771	27.813419043106723	27.69091553233538	17.417694287580193
5	27.70414761886469	31.357057183955884	22.259997695055894	18.678797502123537
6	35.889687082495655	29.24995411453865	20.096124706012915	14.764234096952777
7	76.26462465692615	4.694402827398102	13.355870564583253	5.6851019510925
8	67.56367408090354	8.170813431848352	16.17537209907845	8.090140388169662
9	31.474865438216824	25.564818016126072	22.61875797660055	20.341558569056552
10	24.73759918729378	28.527951715689415	28.956287161888177	17.77816193512863
11	23.10877201968593	21.839558816624624	33.616756089961115	21.43491307372833
12	22.915199399007165	22.41238011089342	35.1623491648846	19.51007132521481
13	20.773735588763824	26.671831448828108	33.344858053858864	19.20957490854921
14	18.23082734688128	25.58125141391247	37.72937395157056	18.45854728763568
15	19.058473017214517	25.364629352182654	33.958229290717476	21.61866833988535
16	18.60858207521801	25.316396262575818	30.931709628263736	25.14331203394244
17	20.614096867410225	24.68979293918789	31.70834773923399	22.987762454167893
18	20.84843285142928	28.018302963560544	31.617217078782133	19.516047106228044
19	21.909560822779678	23.996602341652473	31.16860666128846	22.925230174279385
20	19.73032153970266	29.116992986994138	32.17552576201879	18.977159711284408
21	19.232197508099315	27.127271330336907	30.268184515044027	23.37234664651975
22	17.15475992299779	28.033455836844105	30.672189995688903	24.1395942444692
23	17.515440991296703	25.14992807720709	31.631302848313336	25.703328083182868
24	19.737577845218734	27.481763196872105	30.152297047537335	22.628361910371822
25	17.59590061422492	28.075713145437703	30.28098976007239	24.047396480264982
26	18.87855932456601	27.34047866005916	29.185714468646356	24.595247546728473
27	21.275914614626153	25.336030971619312	32.14970185121159	21.23835256254295
28	19.825934035914443	26.376670550924324	28.77018426747596	25.027211145685275
29	22.678515786732913	24.14108818972251	28.652589433965485	24.52780658957909
30	21.95886101613888	27.527221816722797	26.15471297043269	24.359204196705637
31	25.02443667592913	25.29846891953611	26.556797664323305	23.120296740211458
32	24.643907477836255	26.98129153701356	24.948245468010363	23.42655551713982
33	22.479607647292333	24.93330601547727	28.929822988829557	23.65726334840084
34	24.820833279978146	23.374907695525422	27.46532979908571	24.338929225410727
35	26.450300709837414	24.661194558624548	26.94052817367327	21.947976557864767
36	26.806499942376398	25.761591948061923	26.598628131415964	20.833279978145715
37	23.823731533769564	26.299198818502727	29.696430324527594	20.18063932320012
38	25.537713580816035	24.382680479257637	27.793357492562286	22.28624844736404
39	27.785247504044325	24.717537636749345	28.227882140524756	19.26933271868157
40	23.7592784671268	23.180267971094292	29.451850144485846	23.608603417293057
41	26.505790104960326	23.438507079166293	27.359259686100764	22.69644312977262
42	22.059595610362003	21.94050683159822	31.131898292207154	24.86799926583262
43	24.23947515569044	22.25722322529975	31.92966505747421	21.573636561535604
44	22.92779122328506	23.351217992222946	29.247606486283452	24.473384298208547
45	23.862574110355602	24.818485651722945	26.811195198886793	24.507745039034656
46	26.643873330516175	21.918524494299533	29.74402315188301	21.693579023301275
47	22.953615134092257	22.897058635216986	31.89679826190141	22.252527968789348
48	21.31817192321975	22.227984582484986	31.275957298776248	25.177886195519015
49	23.369572176763604	20.55711352703401	32.85676479752773	23.216549498674656
50	22.758548568160187	20.604066092138005	29.753200244153337	26.884185095548467
51	22.39423934710324	19.276589024197644	30.56611988270396	27.76305174599516
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	304.0
1	429.0
2	554.0
3	3180.0
4	5806.0
5	3909.0
6	2012.0
7	1981.5
8	1951.0
9	2038.0
10	2125.0
11	2101.5
12	2078.0
13	2114.0
14	2150.0
15	2089.0
16	2028.0
17	2089.0
18	2150.0
19	2173.5
20	2197.0
21	2220.5
22	2244.0
23	2354.0
24	2464.0
25	3203.0
26	4586.5
27	5231.0
28	5698.5
29	6166.0
30	6964.5
31	7763.0
32	8446.0
33	9129.0
34	9898.5
35	10668.0
36	11537.0
37	12406.0
38	13138.5
39	13871.0
40	15025.5
41	16180.0
42	18073.5
43	19967.0
44	22621.5
45	25276.0
46	29546.0
47	33816.0
48	45112.5
49	56409.0
50	57910.0
51	59411.0
52	55765.0
53	52119.0
54	41692.0
55	31265.0
56	25576.0
57	19887.0
58	17300.0
59	14713.0
60	13658.0
61	12603.0
62	11261.0
63	9919.0
64	8509.0
65	7099.0
66	5756.5
67	4414.0
68	3653.0
69	2892.0
70	2417.5
71	1943.0
72	1649.0
73	1355.0
74	1145.5
75	752.0
76	568.0
77	441.0
78	314.0
79	232.0
80	150.0
81	111.5
82	73.0
83	40.0
84	7.0
85	4.0
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	468558.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.01695811655341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.79842603400174	34.880129961073294
2	9.982900160057499	9.187653970942005
3	3.8149847064822326	5.266619743604542
4	2.1773249272810147	4.0077547993927265
5	1.541361717602274	3.546438880068134
6	1.137233698883821	3.139922129416194
7	0.919610889627061	2.9622387054046424
8	0.7821199350557744	2.8792624234866433
9	0.6320756213184237	2.617757765343394
>10	3.1289769700829444	21.82484618903788
>50	0.03993846560800993	1.254223520596326
>100	0.03622325950456683	3.3089788708059413
>500	0.004644007628790619	1.355305089037153
>1k	0.0041796068659115575	3.768867951791112
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3543	0.7561497189248716	No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	2333	0.497910610852872	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTC	2285	0.48766641483018114	No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	1853	0.39546865062596304	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCC	1791	0.38223656409665396	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCG	1735	0.3702850020701813	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGC	1609	0.34339398751061767	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	1346	0.2872643301362905	No Hit
TCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	1141	0.24351307628938146	No Hit
ACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	849	0.18119421715134518	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	755	0.16113266660690884	No Hit
TGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCTT	737	0.15729109309839975	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCG	731	0.15601056859556342	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	590	0.1259182427789089	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTC	567	0.12100956551803618	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	539	0.11503378450479984	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT	537	0.11460694300385438	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT	524	0.11183247324770894	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGC	513	0.10948484499250895	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	491	0.10478958848210893	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	477	0.10180169797549077	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4054994258981812	0.0
2	0.0	0.0	0.0	1.6491021389027611	0.0
3	0.0	0.0	0.0	2.4054652785781054	0.0
4	0.0	0.0	0.0	3.318906090601377	0.0
5	0.0	0.0	0.0	5.9373652781512645	0.0
6	0.0	0.0	0.0	7.168589587628426	0.0
7	0.0	0.0	0.0	9.415696669355768	0.0
8	2.1342075047272696E-4	0.0	0.0	11.521305793519693	0.0
9	2.1342075047272696E-4	0.0	0.0	13.84951276042667	0.0
10	2.1342075047272696E-4	0.0	0.0	15.77478135044114	0.0
11	2.1342075047272696E-4	0.0	0.0	18.75093371578332	0.0
12	2.1342075047272696E-4	0.0	0.0	20.995906590005934	0.0
13	2.1342075047272696E-4	0.0	0.0	21.76977023122004	0.0
14	2.1342075047272696E-4	0.0	0.0	22.250393761284624	0.0
15	2.1342075047272696E-4	0.0	0.0	23.275026784304185	0.0
16	2.1342075047272696E-4	0.0	0.0	24.866291899828838	0.0
17	2.1342075047272696E-4	0.0	0.0	27.597650664378797	0.0
18	2.1342075047272696E-4	0.0	0.0	28.549080369986214	0.0
19	2.1342075047272696E-4	0.0	0.0	29.943571553575012	0.0
20	2.1342075047272696E-4	0.0	0.0	30.895641521433845	0.0
21	2.1342075047272696E-4	0.0	0.0	31.95463528527952	0.0
22	2.1342075047272696E-4	0.0	0.0	33.039239539181914	0.0
23	2.1342075047272696E-4	0.0	0.0	33.80670055788184	0.0
24	2.1342075047272696E-4	0.0	0.0	34.51931244371028	0.0
25	2.1342075047272696E-4	0.0	0.0	35.136738674827875	0.0
26	2.1342075047272696E-4	0.0	0.0	35.830142693113764	0.0
27	2.1342075047272696E-4	0.0	0.0	36.515436722881695	0.0
28	2.1342075047272696E-4	0.0	0.0	37.20393206390671	0.0
29	2.1342075047272696E-4	0.0	0.0	37.903525283956306	0.0
30	2.1342075047272696E-4	0.0	0.0	38.61763111503805	0.0
31	2.1342075047272696E-4	0.0	0.0	39.26685703797609	0.0
32	2.1342075047272696E-4	0.0	0.0	39.8904724708574	0.0
33	4.268415009454539E-4	0.0	0.0	40.47695269315645	0.0
34	4.268415009454539E-4	0.0	0.0	41.11081232206045	0.0
35	4.268415009454539E-4	0.0	0.0	41.685127561582554	0.0
36	4.268415009454539E-4	0.0	0.0	42.32026771498939	0.0
37	4.268415009454539E-4	0.0	0.0	42.89671716201623	0.0
38	4.268415009454539E-4	0.0	0.0	43.50560656311492	0.0
39	4.268415009454539E-4	0.0	0.0	44.09528809667106	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGG	25	3.887256E-5	45.0	2
TTAGGCA	20	7.029004E-4	45.0	4
ACCGGAC	20	7.029004E-4	45.0	4
GCAAGCG	35	1.2098644E-7	45.0	1
CGTATGG	20	7.029004E-4	45.0	2
TACTTAG	20	7.029004E-4	45.0	15
CACGGCA	20	7.029004E-4	45.0	4
CGGCATG	20	7.029004E-4	45.0	6
AAAAGCG	20	7.029004E-4	45.0	1
GCACGAG	20	7.029004E-4	45.0	1
TCGATGG	20	7.029004E-4	45.0	2
CGTTAGG	20	7.029004E-4	45.0	2
AACGGAC	25	3.887256E-5	45.0	4
TACGAAT	40	6.7993824E-9	45.0	12
ACGTGGT	20	7.029004E-4	45.0	23
GCTACGA	40	6.7993824E-9	45.0	10
TAAGACG	25	3.887256E-5	45.0	1
CGGAGAT	20	7.029004E-4	45.0	6
TACCGGA	35	1.2098644E-7	45.0	3
TAATCCG	20	7.029004E-4	45.0	1
>>END_MODULE