FastQCFastQC Report
Sat 18 Jun 2016
SRR3551663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470992
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG44710.9492730237456263No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42150.8949196589326358No Hit
GAATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC39880.846723511227367No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC38860.825067092434691No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC26260.5575466249957537TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT25670.5450198729490098TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC20440.43397764717872067TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCC19050.4044654686279172No Hit
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG15550.33015422767265684TruSeq Adapter, Index 22 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC14510.3080731732173795TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAAGGTCCT13090.27792404117267383No Hit
GAACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT11880.25223358358528386No Hit
TGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCTT11130.23630974623772805TruSeq Adapter, Index 20 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCT10750.22824166864829973No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCT10660.22633080816659304No Hit
ACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC9620.20424975371131568TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTA9380.19915412576009783No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTC8380.17792234263002343No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG7840.16645717973978327No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTC7660.16263545877636987No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT7190.15265652070523492No Hit
CGTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT6760.1435268539593029No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC5980.1269660631178449No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGT5850.12420593131093523No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC5670.12038421034752184No Hit
ACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG5610.11911030335971737TruSeq Adapter, Index 22 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG4730.10042633420525189No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA351.2098826E-745.0000046
GTCCGGG351.2098826E-745.0000042
CTCGAAT351.2098826E-745.00000443
GGTACCG207.029042E-445.01
GCAAACG207.029042E-445.01
CCTCGTG406.8012014E-945.015
AACGGTA253.8872877E-545.04
ACGTGCG207.029042E-445.01
TCAGACG253.8872877E-545.021
GAACGGT207.029042E-445.03
TTACGCG207.029042E-445.01
CCGGTAT207.029042E-445.05
CCACGGA207.029042E-445.03
CGATACG207.029042E-445.09
ACGGTAT406.8012014E-945.05
CGGACTA253.8872877E-545.06
AGATACG302.1627638E-644.9999961
TAACGGT302.1627638E-644.9999963
AATAGCG302.1627638E-644.9999961
CGTTTTT33150.043.8461571
Produced by FastQC (version 0.11.5)