##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551663_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	470992
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33146847504841	33.0	31.0	34.0	30.0	34.0
2	32.493452118082686	34.0	31.0	34.0	30.0	34.0
3	32.50304039134423	34.0	31.0	34.0	30.0	34.0
4	36.02424032679961	37.0	35.0	37.0	35.0	37.0
5	36.02592825355845	37.0	35.0	37.0	35.0	37.0
6	36.06120273805075	37.0	35.0	37.0	35.0	37.0
7	36.208965332744505	37.0	36.0	37.0	35.0	37.0
8	35.878736793830896	37.0	35.0	37.0	35.0	37.0
9	37.515074566022356	39.0	37.0	39.0	35.0	39.0
10	37.632762339912354	39.0	37.0	39.0	35.0	39.0
11	37.71847717158678	39.0	37.0	39.0	35.0	39.0
12	37.7381165709821	39.0	37.0	39.0	35.0	39.0
13	37.69273575771987	39.0	37.0	39.0	35.0	39.0
14	39.051888354791586	40.0	38.0	41.0	35.0	41.0
15	39.020123484050686	40.0	38.0	41.0	35.0	41.0
16	39.07873806773788	40.0	39.0	41.0	35.0	41.0
17	39.04503898155383	40.0	38.0	41.0	35.0	41.0
18	38.90250789822333	40.0	38.0	41.0	35.0	41.0
19	38.83774459014166	40.0	38.0	41.0	35.0	41.0
20	38.60404847640724	40.0	38.0	41.0	35.0	41.0
21	38.645724343513265	40.0	38.0	41.0	35.0	41.0
22	38.63946309066821	40.0	38.0	41.0	35.0	41.0
23	38.61335012059653	40.0	38.0	41.0	35.0	41.0
24	38.579710483405236	40.0	38.0	41.0	35.0	41.0
25	38.598417807521145	40.0	38.0	41.0	35.0	41.0
26	38.52027847606753	40.0	38.0	41.0	35.0	41.0
27	38.41396881475694	40.0	38.0	41.0	35.0	41.0
28	38.37784505893943	40.0	38.0	41.0	35.0	41.0
29	38.277656520705236	40.0	37.0	41.0	34.0	41.0
30	38.14055228114278	40.0	37.0	41.0	34.0	41.0
31	37.93299673879811	40.0	37.0	41.0	34.0	41.0
32	37.65960356014539	40.0	37.0	41.0	33.0	41.0
33	37.37498726093012	40.0	37.0	41.0	33.0	41.0
34	37.06862749261134	40.0	37.0	41.0	31.0	41.0
35	36.95556400108707	40.0	37.0	41.0	31.0	41.0
36	36.853738492373544	40.0	37.0	41.0	30.0	41.0
37	36.73832039610014	40.0	37.0	41.0	30.0	41.0
38	36.710491897951556	40.0	37.0	41.0	30.0	41.0
39	36.692557410741585	40.0	37.0	41.0	30.0	41.0
40	36.63998963888983	40.0	36.0	41.0	30.0	41.0
41	36.57268700954581	40.0	36.0	41.0	30.0	41.0
42	36.583330502428915	40.0	36.0	41.0	30.0	41.0
43	36.58784013316574	40.0	36.0	41.0	30.0	41.0
44	36.58042387131841	40.0	36.0	41.0	30.0	41.0
45	36.51484313958623	40.0	36.0	41.0	30.0	41.0
46	36.37309763223155	40.0	36.0	41.0	29.0	41.0
47	36.309958555559334	40.0	35.0	41.0	29.0	41.0
48	36.32773380439583	40.0	35.0	41.0	29.0	41.0
49	36.308669786323335	40.0	35.0	41.0	29.0	41.0
50	36.21358961511024	40.0	35.0	41.0	28.0	41.0
51	35.61242441485206	39.0	35.0	41.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	3.0
13	3.0
14	3.0
15	12.0
16	14.0
17	34.0
18	67.0
19	170.0
20	426.0
21	801.0
22	1378.0
23	2114.0
24	3112.0
25	5017.0
26	6829.0
27	6832.0
28	5793.0
29	5102.0
30	5405.0
31	6199.0
32	7708.0
33	10225.0
34	15811.0
35	22595.0
36	27141.0
37	41766.0
38	86009.0
39	209958.0
40	464.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.86815911947549	22.85113122940517	23.750721880626422	15.529987770492918
2	34.73986819309033	28.248887454563988	21.873195298433945	15.138049053911743
3	26.76563508509699	28.751868396915448	29.396253014913203	15.086243503074362
4	25.115925535890206	26.415735299113365	31.753617895845366	16.714721269151067
5	27.98179162278765	28.89221048340524	24.433748343920918	18.6922495498862
6	32.752573292115365	29.68309440500051	22.527134218840235	15.03719808404389
7	72.84009070217753	3.6026089615110237	17.593080137242247	5.9642201990691985
8	64.4112851173693	9.376167748072154	18.129182661276623	8.083364473281925
9	26.533996331147875	25.550752454394132	25.286841390087307	22.628409824370692
10	23.171518836837993	26.845678907497366	32.35532662975167	17.627475625912968
11	21.236666440194313	20.930928763121244	36.026301932941536	21.80610286374291
12	21.16702619152767	21.52648027991983	37.56815402384754	19.738339504704964
13	20.249388524645852	26.13229099432687	35.94010089343344	17.678219587593844
14	17.058039202364373	26.512552230186497	38.27580935557292	18.15359921187621
15	16.72937119951082	25.102124876855658	36.34732649386826	21.821177429765264
16	15.812582803954205	25.248199544790566	32.13918707748751	26.800030573767707
17	18.557427727010225	24.33459591670347	34.127755885450284	22.98022047083602
18	18.312412949689165	27.661616333186128	34.06045113292795	19.96551958419676
19	18.448933315215545	23.894248734585723	33.67021095899718	23.986606991201548
20	17.50751605122805	28.876498963888984	34.96237728029351	18.65360770458946
21	16.68032578048035	28.04463770085267	32.27443353602609	23.000602982640896
22	14.517019397357068	27.476050548629278	33.23304854434895	24.773881509664708
23	15.672028399633115	25.92995210109726	33.124554132554266	25.27346536671536
24	17.370146414376464	27.86055814111492	31.696504399225468	23.072791045283147
25	15.253337636308048	30.055712198933314	31.505206033223494	23.185744131535145
26	15.708547066616843	29.077351632299486	31.37781533444305	23.83628596664062
27	19.022616095390156	28.22744335360261	32.85512280463362	19.89481774637361
28	16.631492679281177	29.47778306213269	29.676937187892786	24.213787070693346
29	19.139390902605562	28.23105275673472	29.659739443557427	22.969816897102284
30	19.653624690015967	32.191629581818795	27.07285898698916	21.08188674117607
31	19.362112307640047	31.540238475388115	26.249702755036182	22.84794646193566
32	20.27953765669056	31.874851377518088	25.227180079491795	22.618430886299553
33	20.534956007745357	28.042302204708356	26.710432448958795	24.71230933858749
34	19.94577402588579	27.92722594014336	27.913212963277505	24.213787070693346
35	20.890800693005403	29.054421306519007	25.830799673879813	24.22397832659578
36	20.822434351326564	31.609241770560857	25.227180079491795	22.341143798620784
37	19.67761660495295	29.937663484730102	28.57012433332201	21.81459557699494
38	21.691026599177906	26.09980636613785	25.816362061351363	26.39280497333288
39	24.992781193735773	24.345211808268505	27.803444644495023	22.858562353500698
40	19.256802663314875	23.575559669803308	29.847640724258582	27.31999694262323
41	18.346171484865987	28.392201990691984	27.02890919590991	26.232717328532118
42	17.72492951048001	26.807674015694534	28.385195502259066	27.082200971566394
43	22.12288106804362	27.02360125012739	26.867123008458744	23.98639467337025
44	21.60992118762102	24.424830995006285	29.70050446716717	24.264743350205524
45	22.39486020993987	24.216122566837655	27.56012840982437	25.828888813398105
46	22.0602473078099	23.891700920610116	30.67759112681319	23.37046064476679
47	18.631102014471583	24.0021061928865	32.065512790026155	25.301279002615757
48	18.89883479974182	23.15347182117743	29.622159187417196	28.325534191663554
49	20.263826137174306	20.633046845806298	33.53496450045861	25.56816251656079
50	20.657888032068485	20.39992186703808	29.501138023575773	29.441052077317657
51	19.2638091517478	20.209048136698712	30.877382206067196	29.649760505486295
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	187.0
1	313.5
2	440.0
3	3691.5
4	6943.0
5	4824.0
6	2705.0
7	2716.0
8	2727.0
9	2789.0
10	2851.0
11	2777.5
12	2704.0
13	2757.0
14	2810.0
15	2709.5
16	2609.0
17	2512.0
18	2415.0
19	2414.0
20	2413.0
21	2537.0
22	2661.0
23	2801.0
24	2941.0
25	3418.0
26	4492.5
27	5090.0
28	5486.5
29	5883.0
30	6562.5
31	7242.0
32	8361.0
33	9480.0
34	10275.5
35	11071.0
36	12323.0
37	13575.0
38	14646.0
39	15717.0
40	17550.5
41	19384.0
42	22878.0
43	26372.0
44	31247.0
45	36122.0
46	53205.0
47	70288.0
48	68229.5
49	66171.0
50	60439.0
51	54707.0
52	42633.0
53	30559.0
54	24395.0
55	18231.0
56	15121.5
57	12012.0
58	10160.0
59	8308.0
60	7444.0
61	6580.0
62	5699.5
63	4819.0
64	4399.5
65	3980.0
66	3245.5
67	2511.0
68	2134.5
69	1758.0
70	1503.0
71	1248.0
72	993.5
73	739.0
74	581.5
75	327.5
76	231.0
77	177.5
78	124.0
79	88.5
80	53.0
81	28.5
82	4.0
83	4.0
84	4.0
85	2.0
86	0.0
87	1.5
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	470992.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.92854842906458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.60033292736757	38.458001961773036
2	9.778505474100111	9.568961573067607
3	3.500582913100943	5.138353217808462
4	1.8593704369478128	3.639051858870881
5	1.221840338880517	2.9891437096750026
6	0.8738525878618054	2.565380319903585
7	0.6725152092569411	2.3033635089787516
8	0.5590623893173665	2.188328895246666
9	0.45796848133938595	2.016695971637937
>10	2.387774613935607	17.708086290617977
>50	0.04344562950408211	1.4686679536011502
>100	0.033453134717161936	2.9315964318514234
>500	0.004779019245308848	1.6993098307751635
>1k	0.006516844425421157	7.325058476192314
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG	4471	0.9492730237456263	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4215	0.8949196589326358	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC	3988	0.846723511227367	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC	3886	0.825067092434691	No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	2626	0.5575466249957537	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	2567	0.5450198729490098	TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	2044	0.43397764717872067	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCC	1905	0.4044654686279172	No Hit
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG	1555	0.33015422767265684	TruSeq Adapter, Index 22 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	1451	0.3080731732173795	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAAGGTCCT	1309	0.27792404117267383	No Hit
GAACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT	1188	0.25223358358528386	No Hit
TGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCTT	1113	0.23630974623772805	TruSeq Adapter, Index 20 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCT	1075	0.22824166864829973	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCT	1066	0.22633080816659304	No Hit
ACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	962	0.20424975371131568	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTA	938	0.19915412576009783	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTC	838	0.17792234263002343	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG	784	0.16645717973978327	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTC	766	0.16263545877636987	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT	719	0.15265652070523492	No Hit
CGTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT	676	0.1435268539593029	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC	598	0.1269660631178449	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGT	585	0.12420593131093523	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC	567	0.12038421034752184	No Hit
ACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG	561	0.11911030335971737	TruSeq Adapter, Index 22 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG	473	0.10042633420525189	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5566973536705506	0.0
2	0.0	0.0	0.0	2.0991863980704557	0.0
3	0.0	0.0	0.0	3.127653972891259	0.0
4	0.0	0.0	0.0	4.313873696368516	0.0
5	0.0	0.0	0.0	7.55150830587356	0.0
6	0.0	0.0	0.0	9.083593776539729	0.0
7	0.0	0.0	0.0	11.72376600876448	0.0
8	0.0	0.0	0.0	13.893441926826783	0.0
9	0.0	0.0	0.0	16.25335462173455	0.0
10	0.0	0.0	0.0	18.933230288412542	0.0
11	0.0	0.0	0.0	22.22627985188708	0.0
12	0.0	0.0	0.0	25.237371335394233	0.0
13	0.0	0.0	0.0	26.137386622278086	0.0
14	0.0	0.0	0.0	26.711494038115298	0.0
15	0.0	0.0	0.0	27.971175731222612	0.0
16	0.0	0.0	0.0	29.87778985630329	0.0
17	0.0	0.0	0.0	32.83877433162347	0.0
18	0.0	0.0	0.0	33.897391038488976	0.0
19	0.0	0.0	0.0	35.506335564086015	0.0
20	0.0	0.0	0.0	36.596162992152735	0.0
21	0.0	0.0	0.0	37.71274246696335	0.0
22	0.0	0.0	0.0	38.901934640078814	0.0
23	0.0	0.0	0.0	39.75417841492	0.0
24	0.0	0.0	0.0	40.50259877025512	0.0
25	0.0	0.0	0.0	41.16736590005775	0.0
26	0.0	0.0	0.0	41.99689166694976	0.0
27	0.0	0.0	0.0	42.701786866868225	0.0
28	0.0	0.0	0.0	43.39394299690865	0.0
29	0.0	0.0	0.0	44.11837143730679	0.0
30	0.0	0.0	0.0	44.79630227265007	0.0
31	0.0	0.0	0.0	45.42837245643238	0.0
32	0.0	0.0	0.0	46.063202772021604	0.0
33	0.0	0.0	0.0	46.635611645208414	0.0
34	0.0	0.0	0.0	47.2606753405578	0.0
35	0.0	0.0	0.0	47.827776267962086	0.0
36	0.0	0.0	0.0	48.42099228861637	0.0
37	2.1231783130074396E-4	0.0	0.0	48.989367123008456	0.0
38	2.1231783130074396E-4	0.0	0.0	49.56411149233957	0.0
39	2.1231783130074396E-4	0.0	0.0	50.11040527227639	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	35	1.2098826E-7	45.000004	6
GTCCGGG	35	1.2098826E-7	45.000004	2
CTCGAAT	35	1.2098826E-7	45.000004	43
GGTACCG	20	7.029042E-4	45.0	1
GCAAACG	20	7.029042E-4	45.0	1
CCTCGTG	40	6.8012014E-9	45.0	15
AACGGTA	25	3.8872877E-5	45.0	4
ACGTGCG	20	7.029042E-4	45.0	1
TCAGACG	25	3.8872877E-5	45.0	21
GAACGGT	20	7.029042E-4	45.0	3
TTACGCG	20	7.029042E-4	45.0	1
CCGGTAT	20	7.029042E-4	45.0	5
CCACGGA	20	7.029042E-4	45.0	3
CGATACG	20	7.029042E-4	45.0	9
ACGGTAT	40	6.8012014E-9	45.0	5
CGGACTA	25	3.8872877E-5	45.0	6
AGATACG	30	2.1627638E-6	44.999996	1
TAACGGT	30	2.1627638E-6	44.999996	3
AATAGCG	30	2.1627638E-6	44.999996	1
CGTTTTT	3315	0.0	43.846157	1
>>END_MODULE