FastQCFastQC Report
Sat 18 Jun 2016
SRR3551660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441080
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC38520.8733109639974608TruSeq Adapter, Index 16 (100% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC25510.5783531332184638No Hit
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT23930.5425319669901152TruSeq Adapter, Index 16 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC23620.5355037634896165TruSeq Adapter, Index 16 (100% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG21020.47655754058220734No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGC16640.37725582660741813No Hit
TCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC14120.3201233336356217TruSeq Adapter, Index 16 (100% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13090.2967715607146096No Hit
TGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCTT12020.2725129228257912TruSeq Adapter, Index 16 (100% over 25bp)
ACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC11420.25890994830869685TruSeq Adapter, Index 16 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCC10620.24077264895257097No Hit
AGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG7230.16391584293098757TruSeq Adapter, Index 16 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCT6530.14804570599437744No Hit
ACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG6390.1448716786070554TruSeq Adapter, Index 16 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT6110.13852362383241135TruSeq Adapter, Index 16 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG6080.13784347510655662TruSeq Adapter, Index 16 (100% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG5480.12424050058946223TruSeq Adapter, Index 16 (95% over 23bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5380.1219733381699465No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5030.11403826970164144No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTC4940.11199782352407726No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTACCCTCT4510.1022490251201596No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCACG253.8868828E-545.01
GGTACTA207.028554E-445.08
GGTAATC253.8868828E-545.07
CGATTGT207.028554E-445.09
TGCGGAT253.8868828E-545.04
GCCGATG700.045.08
TGACCGA207.028554E-445.026
GTATGCG207.028554E-445.01
CGAATGG253.8868828E-545.02
TTAACGG551.8189894E-1245.02
ATGCGGA502.1827873E-1145.03
AATAGCG253.8868828E-545.01
TATACGG207.028554E-445.02
AAATGCG207.028554E-445.01
CAGGTAC302.1624492E-644.9999965
GTGCAAG302.1624492E-644.9999961
TACCGGA302.1624492E-644.9999963
GGCGATC800.042.18757
GCGATAA650.041.538468
TAACGGA556.002665E-1140.9090923
Produced by FastQC (version 0.11.5)