##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551660_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	441080
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32528792962728	33.0	31.0	34.0	30.0	34.0
2	32.452752335177294	34.0	31.0	34.0	30.0	34.0
3	32.51097986759771	34.0	31.0	34.0	30.0	34.0
4	35.99841298630634	37.0	35.0	37.0	35.0	37.0
5	36.00789199238233	37.0	35.0	37.0	35.0	37.0
6	36.04186088691394	37.0	35.0	37.0	35.0	37.0
7	36.14039403282851	37.0	35.0	37.0	35.0	37.0
8	35.79229391493607	37.0	35.0	37.0	35.0	37.0
9	37.54628412079441	39.0	37.0	39.0	35.0	39.0
10	37.65169130316496	39.0	37.0	39.0	35.0	39.0
11	37.68315044889816	39.0	37.0	39.0	35.0	39.0
12	37.65406502221819	39.0	37.0	39.0	35.0	39.0
13	37.60803028928992	39.0	37.0	39.0	35.0	39.0
14	38.960236238324114	40.0	38.0	41.0	35.0	41.0
15	38.961501314954205	40.0	38.0	41.0	35.0	41.0
16	38.98111226988301	40.0	38.0	41.0	35.0	41.0
17	38.95623469665367	40.0	38.0	41.0	35.0	41.0
18	38.89199238233427	40.0	38.0	41.0	35.0	41.0
19	38.90829101296817	40.0	38.0	41.0	35.0	41.0
20	38.77375759499411	40.0	38.0	41.0	35.0	41.0
21	38.772624013784345	40.0	38.0	41.0	35.0	41.0
22	38.760131948852816	40.0	38.0	41.0	35.0	41.0
23	38.768182642604515	40.0	38.0	41.0	35.0	41.0
24	38.705457059943775	40.0	38.0	41.0	35.0	41.0
25	38.67027750068015	40.0	38.0	41.0	35.0	41.0
26	38.60412850276594	40.0	38.0	41.0	35.0	41.0
27	38.506960188627914	40.0	38.0	41.0	35.0	41.0
28	38.424734741996915	40.0	38.0	41.0	35.0	41.0
29	38.409510746349866	40.0	38.0	41.0	35.0	41.0
30	38.276938423868685	40.0	38.0	41.0	34.0	41.0
31	38.18502312505668	40.0	38.0	41.0	34.0	41.0
32	38.10484492609051	40.0	38.0	41.0	34.0	41.0
33	38.01071460959463	40.0	38.0	41.0	34.0	41.0
34	37.95727985852906	40.0	38.0	41.0	34.0	41.0
35	37.883982497506125	40.0	37.0	41.0	34.0	41.0
36	37.86024984129863	40.0	37.0	41.0	34.0	41.0
37	37.75129681690396	40.0	37.0	41.0	33.0	41.0
38	37.7308084701188	40.0	37.0	41.0	33.0	41.0
39	37.67908542667997	40.0	37.0	41.0	33.0	41.0
40	37.62140201324023	40.0	37.0	41.0	33.0	41.0
41	37.586419697107104	40.0	37.0	41.0	33.0	41.0
42	37.51187766391584	40.0	37.0	41.0	33.0	41.0
43	37.410370000906866	40.0	36.0	41.0	33.0	41.0
44	37.37819216468668	40.0	36.0	41.0	33.0	41.0
45	37.3352112995375	40.0	36.0	41.0	33.0	41.0
46	37.22235422145643	40.0	36.0	41.0	33.0	41.0
47	37.124056860433484	39.0	35.0	41.0	32.0	41.0
48	37.164296726217465	40.0	35.0	41.0	33.0	41.0
49	37.106524893443364	39.0	35.0	41.0	32.0	41.0
50	37.01231976058765	39.0	35.0	41.0	32.0	41.0
51	36.257830778997004	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	2.0
14	5.0
15	3.0
16	16.0
17	27.0
18	56.0
19	132.0
20	294.0
21	570.0
22	889.0
23	1271.0
24	1903.0
25	2381.0
26	2717.0
27	3038.0
28	3177.0
29	3778.0
30	4461.0
31	5637.0
32	7500.0
33	10042.0
34	17626.0
35	24965.0
36	27716.0
37	42553.0
38	81023.0
39	198825.0
40	471.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.06801487258547	22.74371996009794	23.054094495329647	14.13417067198694
2	30.911399292645324	27.317946857712887	22.571415616214747	19.199238233427042
3	30.451618753967537	27.75596263716333	24.634986850457967	17.157431758411175
4	28.18604334814546	28.10215833862338	23.88614310329192	19.825655209939242
5	28.804525256189358	30.107010066201145	19.31259635440283	21.775868323206673
6	37.31840029019679	27.50136029745171	18.11689489435023	17.06334451800127
7	73.2710619388773	5.727078987938696	13.074045524621383	7.927813548562619
8	62.97678425682416	9.447492518364015	16.797633082434025	10.778090142377799
9	31.71714881654122	26.60424412804933	20.378162691575223	21.300444363834224
10	25.536864060941326	25.832275324204225	29.898884556089598	18.73197605876485
11	24.309195610773557	19.503264713884104	32.31409268159971	23.873446993742633
12	23.169039629999094	19.664686678153622	34.762854810918654	22.40341888092863
13	18.58257005531876	26.976058764849913	32.3707717420876	22.07059943774372
14	16.397252199147548	24.24072730570418	37.54035549106738	21.82166500408089
15	17.640337353768025	24.721139022399566	34.27790876938424	23.360614854448173
16	16.99124875306067	24.774644055500136	28.960279314410087	29.273827877029113
17	18.926271878117348	24.612541942504762	29.528656932982678	26.93252924639521
18	20.18296000725492	26.330824340255738	31.559807744626827	21.92640790786252
19	21.6568422961821	22.78248843747166	28.803618391221548	26.757050875124694
20	19.471977872494787	28.71791965176385	30.19633626553006	21.6137662102113
21	18.786161240591277	27.178743085154622	28.299174752879296	25.735920921374806
22	17.092817629454974	27.677065384964177	28.39575587195067	26.83436111363018
23	17.94685771288655	23.475333272875666	30.302892899247304	28.27491611499048
24	20.64546114083613	25.597397297542397	28.163371723950302	25.59376983767117
25	17.07468033009885	27.037725582660745	28.012151990568608	27.875442096671804
26	17.44672168314138	26.40223995647048	27.50861521719416	28.64242314319398
27	20.742722408633355	24.75219914754693	31.62238142740546	22.882697016414255
28	17.72354221456425	26.96585653396209	26.06919379704362	29.241407454430036
29	19.18427496145824	23.26924820894169	26.028838305976237	31.51763852362383
30	20.653622925546387	25.006121338532694	26.213838759408723	28.126416976512196
31	20.921148091049243	25.78829237326562	22.59703455155527	30.693524984129862
32	17.81830960370001	25.490840663825153	25.589915661557995	31.100934070916843
33	17.964768296000724	21.868141833680966	26.661376621021134	33.50571324929718
34	17.518137299356127	21.355763126870407	26.649360660197697	34.476738913575765
35	17.16287294821801	22.086016142196428	28.045705994377435	32.70540491520813
36	19.466083250204044	22.681372993561258	24.992064931531697	32.860478824703
37	17.302076720776277	24.41031105468396	30.415797587739185	27.87181463680058
38	17.637163326380705	23.349505758592546	24.499183821528973	34.514147093497776
39	21.823025301532603	19.353631994196064	27.770019044164325	31.05332366010701
40	17.107554185181826	18.4653577582298	29.68735830234878	34.7397297542396
41	18.912895619842203	21.320395393125963	27.697696562981772	32.06901242405006
42	17.996508569873946	19.985263444273148	32.3136392491158	29.704588736737097
43	21.843883195792145	22.20980321030199	27.650539584655842	28.295774009250025
44	17.884057313865963	20.735467488890905	28.740364559717058	32.64011063752607
45	20.34302167407273	20.21651401106375	26.27346513104199	33.166999183821524
46	21.68767570508751	18.947129772376893	29.849687131586105	29.515507390949487
47	17.897433572141107	19.62727849823161	34.01718509113993	28.45810283848735
48	18.336809649043257	18.867779087693844	29.23369910220368	33.56171216105922
49	19.79096762492065	16.709213748072912	34.91226081436474	28.587557812641695
50	19.851274145279767	17.434252289834042	29.79640881472749	32.9180647501587
51	19.26385236238324	16.487938695928175	30.825020404461778	33.42318853722681
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	324.0
1	484.0
2	644.0
3	1500.5
4	2357.0
5	1624.5
6	892.0
7	893.5
8	895.0
9	923.5
10	952.0
11	969.0
12	986.0
13	1043.5
14	1101.0
15	1072.0
16	1043.0
17	1044.5
18	1046.0
19	1072.0
20	1098.0
21	1192.0
22	1286.0
23	1317.5
24	1349.0
25	1690.5
26	2648.0
27	3264.0
28	3734.5
29	4205.0
30	4649.5
31	5094.0
32	5585.0
33	6076.0
34	6701.5
35	7327.0
36	7886.0
37	8445.0
38	9406.5
39	10368.0
40	11357.5
41	12347.0
42	14157.0
43	15967.0
44	18799.5
45	21632.0
46	26289.0
47	30946.0
48	43552.0
49	56158.0
50	59626.5
51	63095.0
52	59340.0
53	55585.0
54	44359.0
55	33133.0
56	27829.5
57	22526.0
58	19775.0
59	17024.0
60	15488.0
61	13952.0
62	12801.5
63	11651.0
64	10207.5
65	8764.0
66	7051.5
67	5339.0
68	4653.5
69	3968.0
70	3434.0
71	2900.0
72	2475.5
73	2051.0
74	1707.0
75	1139.5
76	916.0
77	686.5
78	457.0
79	384.5
80	312.0
81	222.5
82	133.0
83	96.5
84	60.0
85	36.0
86	12.0
87	7.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	441080.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.80819986090339
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.73896472036056	26.037739513804254
2	11.163620430985153	8.21825543989932
3	4.318121851604274	4.76826876412753
4	2.4013786139585807	3.5356169585714645
5	1.7320679104555148	3.1877150910351952
6	1.2811984592845644	2.8295165370496647
7	1.1394914060085863	2.93598391885021
8	0.9366750575392866	2.7581858178103396
9	0.8664242698140657	2.8702365918888075
>10	5.267823865081796	30.613075109961972
>50	0.08451991136939092	2.151830685050422
>100	0.057991764005275524	4.218051390334697
>500	0.004935469277044726	1.095217327353122
>1k	0.006786270255936498	4.780306854263026
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	3852	0.8733109639974608	TruSeq Adapter, Index 16 (100% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC	2551	0.5783531332184638	No Hit
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	2393	0.5425319669901152	TruSeq Adapter, Index 16 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	2362	0.5355037634896165	TruSeq Adapter, Index 16 (100% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG	2102	0.47655754058220734	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGC	1664	0.37725582660741813	No Hit
TCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	1412	0.3201233336356217	TruSeq Adapter, Index 16 (100% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1309	0.2967715607146096	No Hit
TGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCTT	1202	0.2725129228257912	TruSeq Adapter, Index 16 (100% over 25bp)
ACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	1142	0.25890994830869685	TruSeq Adapter, Index 16 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCC	1062	0.24077264895257097	No Hit
AGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG	723	0.16391584293098757	TruSeq Adapter, Index 16 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCT	653	0.14804570599437744	No Hit
ACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG	639	0.1448716786070554	TruSeq Adapter, Index 16 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT	611	0.13852362383241135	TruSeq Adapter, Index 16 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG	608	0.13784347510655662	TruSeq Adapter, Index 16 (100% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG	548	0.12424050058946223	TruSeq Adapter, Index 16 (95% over 23bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	538	0.1219733381699465	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	503	0.11403826970164144	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTC	494	0.11199782352407726	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTACCCTCT	451	0.1022490251201596	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5550013602974517	0.0
2	0.0	0.0	0.0	2.5949941053777095	0.0
3	0.0	0.0	0.0	3.635168223451528	0.0
4	0.0	0.0	0.0	4.89775097487984	0.0
5	0.0	0.0	0.0	8.505033100571325	0.0
6	0.0	0.0	0.0	10.084565158247937	0.0
7	0.0	0.0	0.0	13.09218282397751	0.0
8	0.0	0.0	0.0	15.692391402920105	0.0
9	0.0	0.0	0.0	18.087195066654576	0.0
10	0.0	0.0	0.0	20.19588283304616	0.0
11	0.0	0.0	0.0	23.453795229890268	0.0
12	0.0	0.0	0.0	25.990523261086423	0.0
13	0.0	0.0	0.0	26.77541489072277	0.0
14	0.0	0.0	0.0	27.313185816631904	0.0
15	0.0	0.0	0.0	28.44087240409903	0.0
16	0.0	0.0	0.0	30.081617847102567	0.0
17	0.0	0.0	0.0	33.03391674979596	0.0
18	0.0	0.0	0.0	33.977509748798404	0.0
19	2.267162419515734E-4	0.0	0.0	35.316042441280494	0.0
20	2.267162419515734E-4	0.0	0.0	36.21701278679605	0.0
21	2.267162419515734E-4	0.0	0.0	37.18373084247756	0.0
22	4.534324839031468E-4	0.0	0.0	38.08583476920287	0.0
23	4.534324839031468E-4	0.0	0.0	38.77686587467126	0.0
24	4.534324839031468E-4	0.0	0.0	39.39920195882833	0.0
25	4.534324839031468E-4	0.0	0.0	39.94400108823796	0.0
26	4.534324839031468E-4	0.0	0.0	40.6048789335268	0.0
27	4.534324839031468E-4	0.0	0.0	41.18028475559989	0.0
28	4.534324839031468E-4	0.0	0.0	41.7434479006076	0.0
29	4.534324839031468E-4	0.0	0.0	42.349913847828056	0.0
30	4.534324839031468E-4	0.0	0.0	42.92781354856262	0.0
31	6.801487258547202E-4	0.0	0.0	43.49347057223179	0.0
32	6.801487258547202E-4	0.0	0.0	44.03577582297996	0.0
33	6.801487258547202E-4	0.0	0.0	44.56243765303346	0.0
34	6.801487258547202E-4	0.0	0.0	45.12061304071824	0.0
35	6.801487258547202E-4	0.0	0.0	45.603065203591186	0.0
36	6.801487258547202E-4	0.0	0.0	46.11929808651492	0.0
37	6.801487258547202E-4	0.0	0.0	46.57953205767661	0.0
38	6.801487258547202E-4	0.0	0.0	47.06629182914664	0.0
39	6.801487258547202E-4	0.0	0.0	47.562573682778634	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCACG	25	3.8868828E-5	45.0	1
GGTACTA	20	7.028554E-4	45.0	8
GGTAATC	25	3.8868828E-5	45.0	7
CGATTGT	20	7.028554E-4	45.0	9
TGCGGAT	25	3.8868828E-5	45.0	4
GCCGATG	70	0.0	45.0	8
TGACCGA	20	7.028554E-4	45.0	26
GTATGCG	20	7.028554E-4	45.0	1
CGAATGG	25	3.8868828E-5	45.0	2
TTAACGG	55	1.8189894E-12	45.0	2
ATGCGGA	50	2.1827873E-11	45.0	3
AATAGCG	25	3.8868828E-5	45.0	1
TATACGG	20	7.028554E-4	45.0	2
AAATGCG	20	7.028554E-4	45.0	1
CAGGTAC	30	2.1624492E-6	44.999996	5
GTGCAAG	30	2.1624492E-6	44.999996	1
TACCGGA	30	2.1624492E-6	44.999996	3
GGCGATC	80	0.0	42.1875	7
GCGATAA	65	0.0	41.53846	8
TAACGGA	55	6.002665E-11	40.909092	3
>>END_MODULE