##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551657_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	416457
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50942114071801	34.0	31.0	34.0	31.0	34.0
2	32.65430764760828	34.0	31.0	34.0	31.0	34.0
3	32.68009662462151	34.0	31.0	34.0	31.0	34.0
4	36.12976369709238	37.0	37.0	37.0	35.0	37.0
5	36.12569845146078	37.0	35.0	37.0	35.0	37.0
6	36.16120751962387	37.0	37.0	37.0	35.0	37.0
7	36.23891782344876	37.0	37.0	37.0	35.0	37.0
8	35.86711233092492	37.0	36.0	37.0	35.0	37.0
9	37.657136270971556	39.0	37.0	39.0	35.0	39.0
10	37.74135865167352	39.0	37.0	39.0	35.0	39.0
11	37.76825218449924	39.0	37.0	39.0	35.0	39.0
12	37.76004245336253	39.0	38.0	39.0	35.0	39.0
13	37.71632365406273	39.0	38.0	39.0	35.0	39.0
14	39.129182604686676	40.0	39.0	41.0	35.0	41.0
15	39.13139411751994	40.0	39.0	41.0	35.0	41.0
16	39.148108928412775	40.0	39.0	41.0	35.0	41.0
17	39.11186028809697	40.0	39.0	41.0	35.0	41.0
18	39.04329138422454	40.0	38.0	41.0	35.0	41.0
19	38.995973173701	40.0	38.0	41.0	35.0	41.0
20	38.83964971173494	40.0	38.0	41.0	35.0	41.0
21	38.83557726247848	40.0	38.0	41.0	35.0	41.0
22	38.804911431432295	40.0	38.0	41.0	35.0	41.0
23	38.77672124613105	40.0	38.0	41.0	35.0	41.0
24	38.74208141536821	40.0	38.0	41.0	35.0	41.0
25	38.71766833070401	40.0	38.0	41.0	35.0	41.0
26	38.644318621130154	40.0	38.0	41.0	35.0	41.0
27	38.512677179156555	40.0	38.0	41.0	35.0	41.0
28	38.464300035778	40.0	38.0	41.0	35.0	41.0
29	38.4193590214596	40.0	38.0	41.0	35.0	41.0
30	38.28110705306911	40.0	38.0	41.0	34.0	41.0
31	38.1743205180847	40.0	38.0	41.0	34.0	41.0
32	37.98300905015404	40.0	38.0	41.0	34.0	41.0
33	37.81300350336289	40.0	37.0	41.0	33.0	41.0
34	37.66532679244196	40.0	37.0	41.0	33.0	41.0
35	37.56145772552748	40.0	37.0	41.0	33.0	41.0
36	37.48738044984236	40.0	37.0	41.0	33.0	41.0
37	37.36485879694662	40.0	37.0	41.0	33.0	41.0
38	37.32259753107764	40.0	37.0	41.0	33.0	41.0
39	37.22015238067796	40.0	37.0	41.0	32.0	41.0
40	37.15836208780258	40.0	36.0	41.0	32.0	41.0
41	37.11727741399472	40.0	36.0	41.0	32.0	41.0
42	37.05149391173639	40.0	36.0	41.0	32.0	41.0
43	36.94064453232867	40.0	36.0	41.0	32.0	41.0
44	36.9028471126671	40.0	35.0	41.0	31.0	41.0
45	36.85436431612387	40.0	35.0	41.0	31.0	41.0
46	36.74878558890834	40.0	35.0	41.0	31.0	41.0
47	36.619557361264185	39.0	35.0	41.0	31.0	41.0
48	36.59543002038626	39.0	35.0	41.0	31.0	41.0
49	36.48797594949779	39.0	35.0	41.0	31.0	41.0
50	36.34915009232646	39.0	35.0	41.0	31.0	41.0
51	35.635715091834214	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	3.0
13	2.0
14	3.0
15	6.0
16	12.0
17	45.0
18	80.0
19	204.0
20	410.0
21	786.0
22	1239.0
23	1775.0
24	2590.0
25	3256.0
26	3703.0
27	3911.0
28	3814.0
29	3770.0
30	4133.0
31	5063.0
32	6541.0
33	8620.0
34	15394.0
35	21966.0
36	24806.0
37	38209.0
38	72794.0
39	192631.0
40	690.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.56014666580223	23.327018155535868	22.879673051479504	14.233162127182396
2	34.079388748418204	27.557947159010414	21.851475662553398	16.511188430017985
3	31.67649961460607	28.265583241487118	24.243559359069483	15.81435778483733
4	28.561892344227612	28.288874961880815	24.793916298681495	18.35531639521007
5	29.9269792559616	31.55067630031432	19.19934110844577	19.32300333527831
6	39.33755465750365	29.50724804721736	16.782765087391976	14.372432207887009
7	76.871081528225	5.817647440191904	11.962819690868445	5.3484513407146475
8	67.82308857817254	10.579723716974382	13.842485538723084	7.754702166129997
9	32.55270051890111	28.821943201819156	19.180131442141686	19.44522483713805
10	26.83614394763445	27.670083586060507	27.760849259347303	17.73292320695774
11	25.29024605181327	22.351887469774788	31.37298688700154	20.984879591410397
12	24.439257834542342	21.830104908790105	32.22973800416369	21.50089925250386
13	21.706922923615164	27.198486278295235	30.6807185375681	20.413872260521497
14	18.952256775609484	26.08552623680236	35.237971747383284	19.724245240204873
15	20.532011708291613	24.952396045690193	32.89631342491542	21.619278821102778
16	19.85895302516226	24.508652754065846	29.18380529082234	26.44858892994955
17	21.30880258946302	25.088784676449187	30.11595434822803	23.486458385859766
18	22.300021370753765	26.158042727100277	30.183428301121122	21.358507601024836
19	23.4715708944741	23.593792396333836	29.343245521146237	23.591391188045822
20	22.26448348809121	27.44989278604994	30.22592968781891	20.05969403803994
21	20.149018986353934	27.987523321735498	28.783523869210985	23.079933822699584
22	19.569127184799388	27.173753832928732	29.278412897369954	23.978706084901923
23	19.857512300189455	24.775426994863817	30.170942018023467	25.196118686923256
24	20.300535229327398	27.717387389334313	28.87933207990261	23.102745301435682
25	19.274979169518105	28.189705059585986	28.367154352069964	24.168161418825953
26	19.52110301903918	27.68665192324778	28.962654007496575	23.82959105021647
27	21.79072509286673	26.807329448178326	31.021209872807997	20.38073558614695
28	19.994621293434854	28.18130083057795	27.682569869158158	24.14150800682904
29	22.440251934773578	25.441041932300333	28.41998093440619	23.698725198519895
30	21.1901829000353	29.950270976355302	26.057912341490237	22.80163378211916
31	23.43243119937953	29.327397546445372	24.247881533987904	22.9922897201872
32	21.926153240310526	29.843177086710032	25.454008457055586	22.776661215923852
33	22.09255697466965	26.942037233135714	26.590980581428575	24.374425210766056
34	21.61999918358918	27.84201009948206	26.431540351104676	24.106450365824085
35	22.80811704449679	27.233063677642587	25.676120223696564	24.282699054164055
36	24.394835481214148	29.633311482337916	24.606622052216675	21.365230984231264
37	20.906840322050055	29.311069330086898	27.12741051297013	22.654679834892917
38	21.407252129271452	27.543539909282348	25.35531879641836	25.693889165027844
39	24.307671620359365	25.332267196853458	27.34808155463829	23.011979628148886
40	20.68160698463467	25.132966908948585	27.516406255627835	26.669019850788917
41	23.56065572195929	27.206410265645676	24.802320527689535	24.430613484705503
42	22.205173643377346	26.03149905032212	27.912365502320768	23.85096180397976
43	24.268772046093595	25.441282053129132	27.580758637746516	22.70918726303076
44	20.473422226064155	25.50827576436463	29.012118898229588	25.006183111341628
45	21.326331409965494	25.755840338858516	26.27330072492478	26.64452752625121
46	22.8787125681643	26.056231495688632	28.40581380550693	22.659242130640138
47	21.13183353863664	24.29974763300893	30.03287254146286	24.535546286891563
48	21.508823239854294	25.282562185291642	28.07468718259028	25.13392739226379
49	21.435106145412373	22.5367805079516	31.35401734152626	24.67409600510977
50	21.513385535601515	23.142125117359054	28.068444041041452	27.276045305997975
51	21.70836364858797	21.856037958300618	29.18116396170553	27.254434431405883
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	363.0
1	575.5
2	788.0
3	2614.0
4	4440.0
5	2939.5
6	1439.0
7	1520.5
8	1602.0
9	1613.0
10	1624.0
11	1635.0
12	1646.0
13	1604.0
14	1562.0
15	1516.0
16	1470.0
17	1440.5
18	1411.0
19	1430.0
20	1449.0
21	1612.0
22	1775.0
23	1871.0
24	1967.0
25	2807.0
26	4209.0
27	4771.0
28	5496.0
29	6221.0
30	6738.0
31	7255.0
32	8037.5
33	8820.0
34	9494.0
35	10168.0
36	10989.5
37	11811.0
38	12438.5
39	13066.0
40	14998.0
41	16930.0
42	19366.0
43	21802.0
44	24236.5
45	26671.0
46	35167.5
47	43664.0
48	43365.0
49	43066.0
50	41593.0
51	40120.0
52	34238.0
53	28356.0
54	25120.5
55	21885.0
56	19291.5
57	16698.0
58	15947.0
59	15196.0
60	14516.5
61	13837.0
62	12498.0
63	11159.0
64	9668.5
65	8178.0
66	6963.0
67	5748.0
68	5247.0
69	4746.0
70	4308.5
71	3871.0
72	3255.0
73	2639.0
74	2237.5
75	1564.5
76	1293.0
77	995.5
78	698.0
79	559.0
80	420.0
81	315.0
82	210.0
83	137.5
84	65.0
85	60.0
86	55.0
87	32.5
88	10.0
89	6.5
90	3.0
91	3.5
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	416457.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.1854212670104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.79764802310137	27.41623435742662
2	9.605965859410968	7.336157060362549
3	3.8084379601882627	4.362804236371877
4	2.31302237620477	3.532949353416022
5	1.8989281211582103	3.6255685131099407
6	1.5104303096843357	3.4605850601854393
7	1.3276435755083265	3.5487640456257763
8	1.1211809166269695	3.425021249434695
9	0.9986289304506543	3.4319759758814032
>10	5.516806512935813	31.649420754203305
>50	0.05411451966962018	1.4361380825212249
>100	0.03964203185100083	3.063056428974498
>500	0.0031461930040476843	0.7809015841047315
>1k	0.004404670205666759	2.9304232983819403
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2633	0.6322381422331718	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTC	1864	0.4475852248851622	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG	1859	0.4463846207411569	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGC	1690	0.40580420067377904	No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	1491	0.35802015574236956	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCC	1425	0.34217218104150005	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	1234	0.29630910274049904	No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	869	0.20866500022811477	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	699	0.16784445933193584	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	595	0.14287189313662635	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	547	0.13134609335417582	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAATCACGT	540	0.12966524755256845	No Hit
TGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTT	496	0.11909993108532213	Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	490	0.11765920611251583	No Hit
GAACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT	474	0.11381727285169899	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG	456	0.10949509793328004	No Hit
TCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	433	0.10397231887085581	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.401208288010527E-4	0.0	0.0	0.3035127276045306	0.0
2	2.401208288010527E-4	0.0	0.0	1.1091181082320625	0.0
3	2.401208288010527E-4	0.0	0.0	1.5739920327909003	0.0
4	2.401208288010527E-4	0.0	0.0	2.168050963244705	0.0
5	2.401208288010527E-4	0.0	0.0	4.013379532580795	0.0
6	2.401208288010527E-4	0.0	0.0	4.784647634689776	0.0
7	2.401208288010527E-4	0.0	0.0	6.336068309573378	0.0
8	2.401208288010527E-4	0.0	0.0	7.687228213236901	0.0
9	2.401208288010527E-4	0.0	0.0	9.142840677428882	0.0
10	2.401208288010527E-4	0.0	0.0	10.67072951108998	0.0
11	2.401208288010527E-4	0.0	0.0	12.925223972703064	0.0
12	2.401208288010527E-4	0.0	0.0	14.817135982826558	0.0
13	4.802416576021054E-4	0.0	0.0	15.407352980019546	0.0
14	4.802416576021054E-4	0.0	0.0	15.763212048302705	0.0
15	4.802416576021054E-4	0.0	0.0	16.608917607340015	0.0
16	4.802416576021054E-4	0.0	0.0	17.760296981441062	0.0
17	7.203624864031581E-4	0.0	0.0	19.830378646534935	0.0
18	7.203624864031581E-4	0.0	0.0	20.53153146663401	0.0
19	7.203624864031581E-4	0.0	0.0	21.634166312488443	0.0
20	7.203624864031581E-4	0.0	0.0	22.33748022004673	0.0
21	7.203624864031581E-4	0.0	0.0	23.108268080498107	0.0
22	7.203624864031581E-4	0.0	0.0	23.80725981313797	0.0
23	7.203624864031581E-4	0.0	0.0	24.35713651109238	0.0
24	7.203624864031581E-4	0.0	0.0	24.85394650588176	0.0
25	7.203624864031581E-4	0.0	0.0	25.320501276242204	0.0
26	7.203624864031581E-4	0.0	0.0	25.90543561520157	0.0
27	7.203624864031581E-4	0.0	0.0	26.385677272803676	0.0
28	7.203624864031581E-4	0.0	0.0	26.853912888965727	0.0
29	7.203624864031581E-4	0.0	0.0	27.38169847067044	0.0
30	7.203624864031581E-4	0.0	0.0	27.86746290733497	0.0
31	7.203624864031581E-4	0.0	0.0	28.348905169081082	0.0
32	7.203624864031581E-4	0.0	0.0	28.796970635623847	0.0
33	7.203624864031581E-4	0.0	0.0	29.23543126901457	0.0
34	7.203624864031581E-4	0.0	0.0	29.71447232247267	0.0
35	7.203624864031581E-4	0.0	0.0	30.178385763716303	0.0
36	7.203624864031581E-4	0.0	0.0	30.641338721644733	0.0
37	7.203624864031581E-4	0.0	0.0	31.087963463214688	0.0
38	7.203624864031581E-4	0.0	0.0	31.58765490794968	0.0
39	7.203624864031581E-4	0.0	0.0	32.03403952869084	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCCA	20	7.0281007E-4	45.0	14
CGGCATA	25	3.8865066E-5	45.0	6
CGATTTA	20	7.0281007E-4	45.0	9
CCGTCGA	20	7.0281007E-4	45.0	41
CGGATTA	25	3.8865066E-5	45.0	6
TCTCGTA	25	3.8865066E-5	45.0	27
ACAGACG	20	7.0281007E-4	45.0	1
TGTAGCG	45	3.8380676E-10	45.0	1
TGGCTAG	35	1.2094279E-7	45.0	1
CGTTTTT	1385	0.0	41.588448	1
GACCTAT	55	6.002665E-11	40.909092	33
TGATTCA	50	1.0786607E-9	40.5	44
ACGGATT	50	1.0786607E-9	40.5	5
TCGTTGA	40	3.4511504E-7	39.375	23
TTAATCG	40	3.4511504E-7	39.375	19
CGGATTC	40	3.4511504E-7	39.375	6
ACGGAAT	155	0.0	39.19355	5
TACGGAT	75	0.0	39.0	4
TAAGCGG	105	0.0	38.57143	2
ATTACGG	35	6.238699E-6	38.571426	2
>>END_MODULE