FastQCFastQC Report
Sat 18 Jun 2016
SRR3551656_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551656_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472782
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68521.4492937548383822No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCC25270.5344958141384402No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT21800.46110046490771645No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC20100.4251430892039037No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC19260.4073759153267256No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCG11590.24514469671011166No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCT11550.24429864081119843No Hit
TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC10940.23139628835277146No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTC10560.22335875731309568No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG9710.20538006946118928No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC8810.18634381173564138No Hit
ACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC8740.1848632139125432No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGC8600.18190201826634686No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGT8480.17936385056960713No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT7520.15905850899568935No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC6220.131561692281009No Hit
TGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCTT5650.11950539572149531Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCG4910.10385336159160037No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCT750.045.0000041
CAACGGT207.029069E-445.03
CGACCAA207.029069E-445.019
TACGGTA253.88731E-545.04
ATATGCG253.88731E-545.01
CGCGAGG253.88731E-545.02
TAACGGA253.88731E-545.03
TCTAGCG253.88731E-545.01
CGTAAGG406.8012014E-945.02
ATGCGAG253.88731E-545.01
TCGAGCG207.029069E-445.01
TACCGGA207.029069E-445.03
CACGGAT302.1627802E-644.9999964
GTTAGCG302.1627802E-644.9999961
CGTTTTT52750.043.4644551
TGACGGC650.041.5384643
CGGAATC603.6379788E-1241.2499966
GCGATTA556.002665E-1140.9090928
GCTAGCG556.002665E-1140.9090921
CAAGCGG556.002665E-1140.9090922
Produced by FastQC (version 0.11.5)