##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551656_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	472782
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.413114289461106	33.0	31.0	34.0	30.0	34.0
2	32.59730065865452	34.0	31.0	34.0	31.0	34.0
3	32.56573008278657	34.0	31.0	34.0	31.0	34.0
4	36.0754787618818	37.0	35.0	37.0	35.0	37.0
5	36.1020237657102	37.0	35.0	37.0	35.0	37.0
6	36.14575851026477	37.0	36.0	37.0	35.0	37.0
7	36.29072807340381	37.0	37.0	37.0	35.0	37.0
8	36.076261363588294	37.0	37.0	37.0	35.0	37.0
9	37.631720327762054	39.0	37.0	39.0	35.0	39.0
10	37.761304364379356	39.0	37.0	39.0	35.0	39.0
11	37.823017796785834	39.0	38.0	39.0	35.0	39.0
12	37.833066825725176	39.0	38.0	39.0	35.0	39.0
13	37.82720577348545	39.0	38.0	39.0	35.0	39.0
14	39.211803748873685	40.0	39.0	41.0	36.0	41.0
15	39.21778113379951	40.0	39.0	41.0	36.0	41.0
16	39.21839452432622	41.0	39.0	41.0	36.0	41.0
17	39.208944079935364	40.0	39.0	41.0	36.0	41.0
18	38.994162214297496	40.0	39.0	41.0	36.0	41.0
19	38.8527418556544	40.0	37.0	41.0	35.0	41.0
20	38.611510167476766	40.0	37.0	41.0	35.0	41.0
21	38.57362166918368	40.0	37.0	41.0	35.0	41.0
22	38.55980980663392	40.0	37.0	41.0	35.0	41.0
23	38.58472403771717	40.0	37.0	41.0	35.0	41.0
24	38.53707205435063	40.0	37.0	41.0	35.0	41.0
25	38.51618293420646	40.0	37.0	41.0	35.0	41.0
26	38.47248203188785	40.0	37.0	41.0	35.0	41.0
27	38.419392024231044	40.0	37.0	41.0	35.0	41.0
28	38.27561963018896	40.0	37.0	41.0	35.0	41.0
29	38.2017526047946	40.0	37.0	41.0	35.0	41.0
30	38.008507092063574	40.0	37.0	41.0	34.0	41.0
31	37.68217064101425	40.0	36.0	41.0	33.0	41.0
32	37.333500429373366	40.0	36.0	41.0	33.0	41.0
33	36.85681561480767	40.0	36.0	41.0	31.0	41.0
34	36.49130254535917	40.0	36.0	41.0	30.0	41.0
35	36.27151202879974	40.0	36.0	41.0	27.0	41.0
36	36.17722755942485	40.0	36.0	41.0	25.0	41.0
37	36.0266613365145	40.0	35.0	41.0	24.0	41.0
38	35.99674903020843	40.0	35.0	41.0	24.0	41.0
39	36.06525417634343	40.0	36.0	41.0	24.0	41.0
40	36.09873895368267	40.0	36.0	41.0	24.0	41.0
41	36.05414757753045	40.0	36.0	41.0	24.0	41.0
42	35.93304313616001	40.0	35.0	41.0	23.0	41.0
43	35.82967625671028	40.0	35.0	41.0	23.0	41.0
44	35.81439437203616	40.0	35.0	41.0	23.0	41.0
45	35.80402807213473	40.0	35.0	41.0	23.0	41.0
46	35.74221311302038	40.0	35.0	41.0	23.0	41.0
47	35.64684780723462	40.0	35.0	41.0	23.0	41.0
48	35.585608166131536	39.0	35.0	41.0	23.0	41.0
49	35.54714223468745	39.0	35.0	41.0	23.0	41.0
50	35.46457140923301	39.0	35.0	41.0	22.0	41.0
51	34.81018101365957	39.0	35.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	2.0
13	4.0
14	5.0
15	10.0
16	18.0
17	38.0
18	97.0
19	182.0
20	371.0
21	609.0
22	1036.0
23	1729.0
24	3400.0
25	6352.0
26	9623.0
27	9683.0
28	8124.0
29	6609.0
30	6158.0
31	6512.0
32	7596.0
33	9860.0
34	16237.0
35	21761.0
36	26356.0
37	39514.0
38	83882.0
39	206528.0
40	485.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.4750476964013	22.988607857321135	23.571540371672356	19.96480407460521
2	40.26697293890207	22.535544923453095	21.557504304309386	15.639977833335447
3	27.934227614418482	23.102190861750234	33.649123697602704	15.314457826228578
4	25.905174054849805	26.271093231129782	31.721385331928882	16.102347382091533
5	25.565694125410865	29.12039798469485	28.258267023702256	17.05564086619203
6	33.408632308336614	26.50820039680022	25.770016624998416	14.313150669864758
7	72.0649263296826	4.259256909103985	18.270365623056716	5.405451138156698
8	64.87937358021244	6.040627604265813	21.377505911815593	7.7024929037061485
9	27.97187710192012	22.49683786607781	30.16125825433286	19.3700267776692
10	23.020546467505106	26.862486304470135	33.17300574048927	16.943961487535482
11	19.712890930703793	21.20406445253838	37.91007271850451	21.17297189825332
12	20.687335812277116	20.64820572695238	40.529250267565175	18.135208193205322
13	19.813571582674466	24.504317000224205	37.94687615010724	17.73523526699409
14	17.651687246976408	23.92540325139282	40.91039845002559	17.512511051605177
15	17.76527025140551	23.8490467065159	38.12983573824722	20.255847303831366
16	17.124171394004	24.0903841516809	36.75689852828577	22.028545926029334
17	18.71539102588508	23.45563071352124	36.41445740320063	21.41452085739305
18	18.571773037044558	26.80114725179893	36.906650422393405	17.72042928876311
19	19.249252298099336	23.296360690550824	36.6458536915534	20.80853331979644
20	17.34097321810052	28.577018583617818	35.9047087241054	18.17729947417626
21	16.99683998121756	25.54094699036765	35.571997241857765	21.890215786557018
22	15.490014425253076	27.561116962997744	34.575766420887426	22.37310219086175
23	15.766886218172434	24.147492924857545	35.69340626335182	24.3922145936182
24	17.764847223456055	26.774496490983157	34.79849063627634	20.662165649284447
25	16.565562986746535	25.67377776649703	35.48654559606753	22.2741136506889
26	18.061601329999874	26.017276461455808	33.84921591769568	22.071906290848638
27	20.503318654263484	23.867025394367804	36.833043559187956	18.79661239218075
28	18.69804687995736	24.901751758738698	33.320219466900184	23.079981894403765
29	20.503530168238214	24.022699679767843	33.895325964186455	21.578444187807488
30	22.120766019010876	24.676912403602504	32.58668900254239	20.61563257484422
31	21.345355787656892	24.44128583575517	33.30985528213849	20.903503094449448
32	20.13993764568025	26.904577585441068	30.972414347415935	21.98307042146275
33	20.834338024713293	23.984627164316745	34.19567580830065	20.985359002669306
34	19.856297405569588	24.550427046714976	33.59243795237551	22.000837595339924
35	21.487281664699587	24.50622062599676	32.78424305493864	21.222254654365013
36	22.421115863125078	27.41390323658684	31.311259734930687	18.853721165357396
37	18.61640248571223	26.50100892165945	35.613877008853976	19.26871158377434
38	17.735658294943548	27.457263601406147	32.47606719375949	22.331010909890818
39	20.42167426001836	25.556810538472273	33.201771641052325	20.81974356045704
40	19.127631762630557	23.81118570503953	34.29931765591753	22.761864876412385
41	21.473110228392787	24.605632194119064	30.815047950218073	23.106209627270076
42	19.994204517092445	25.64035855848996	31.844697979195484	22.52073894522211
43	21.786996966889603	24.76511373106421	33.554365436924414	19.893523865121768
44	19.14730256228029	24.824760671937597	33.59539914802171	22.432537617760406
45	21.11628615302613	22.737540769318628	32.786358194685924	23.359814882969317
46	21.276825259844916	22.516508665727546	34.60178263977901	21.604883434648528
47	19.315244658214567	23.625899463177532	34.08949579298704	22.969360085620856
48	18.220659838995562	23.91165484303548	33.199233473355584	24.668451844613372
49	20.201488212326186	20.812763599291006	35.469835992064	23.515912196318812
50	19.95401686189407	20.647571185028195	33.10214855895529	26.296263394122448
51	18.89179368080849	19.872795495598396	34.077227982452804	27.158182841140317
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	263.0
1	415.5
2	568.0
3	6294.5
4	12021.0
5	8133.0
6	4245.0
7	4258.5
8	4272.0
9	4387.0
10	4502.0
11	4423.0
12	4344.0
13	4228.0
14	4112.0
15	3825.0
16	3538.0
17	3477.5
18	3417.0
19	3169.0
20	2921.0
21	3010.0
22	3099.0
23	3157.5
24	3216.0
25	3627.5
26	4560.0
27	5081.0
28	5443.0
29	5805.0
30	6511.0
31	7217.0
32	8029.5
33	8842.0
34	9749.5
35	10657.0
36	11886.0
37	13115.0
38	14966.5
39	16818.0
40	20024.5
41	23231.0
42	27812.0
43	32393.0
44	42158.0
45	51923.0
46	57238.5
47	62554.0
48	59441.5
49	56329.0
50	46328.0
51	36327.0
52	30279.0
53	24231.0
54	20766.0
55	17301.0
56	14921.0
57	12541.0
58	11247.0
59	9953.0
60	8856.0
61	7759.0
62	6622.5
63	5486.0
64	4624.0
65	3762.0
66	3123.5
67	2485.0
68	1960.5
69	1436.0
70	1261.0
71	1086.0
72	903.5
73	721.0
74	622.0
75	419.5
76	316.0
77	244.0
78	172.0
79	125.0
80	78.0
81	62.5
82	47.0
83	34.0
84	21.0
85	14.5
86	8.0
87	6.5
88	5.0
89	3.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	472782.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.085373593945526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.29024353076024	40.49896079403493
2	10.653983531487201	11.311413920654823
3	4.515626243846195	7.191411184956007
4	2.6588864889151527	5.6459193043182
5	1.6686764569997958	4.429115656362777
6	1.165102824284126	3.7109951221490336
7	0.7921260362593361	2.9435214597822665
8	0.5610290300883848	2.382594852743265
9	0.37179035196924093	1.7762966759620489
>10	1.2507561237834632	10.261701026622907
>50	0.03628846515418694	1.3442368937681475
>100	0.0287117526358993	2.929589663950264
>500	0.0031901947373221442	1.3491057828134283
>1k	0.0031901947373221442	2.774631962236875
>5k	3.9877434216526803E-4	1.4505056996450039
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6852	1.4492937548383822	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCC	2527	0.5344958141384402	No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	2180	0.46110046490771645	No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	2010	0.4251430892039037	No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1926	0.4073759153267256	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCG	1159	0.24514469671011166	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCT	1155	0.24429864081119843	No Hit
TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1094	0.23139628835277146	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTC	1056	0.22335875731309568	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG	971	0.20538006946118928	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC	881	0.18634381173564138	No Hit
ACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	874	0.1848632139125432	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGC	860	0.18190201826634686	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGT	848	0.17936385056960713	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT	752	0.15905850899568935	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC	622	0.131561692281009	No Hit
TGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCTT	565	0.11950539572149531	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCG	491	0.10385336159160037	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.47146464966940366	0.0
2	0.0	0.0	0.0	1.748374515104213	0.0
3	0.0	0.0	0.0	2.5149011595196096	0.0
4	0.0	0.0	0.0	3.3656103658768735	0.0
5	0.0	0.0	0.0	5.811346455660326	0.0
6	0.0	0.0	0.0	7.269523797437297	0.0
7	0.0	0.0	0.0	9.624520392062303	0.0
8	0.0	0.0	0.0	11.905487095532402	0.0
9	0.0	0.0	0.0	14.432021523662069	0.0
10	0.0	0.0	0.0	16.33289761454539	0.0
11	0.0	0.0	0.0	19.687720767711124	0.0
12	0.0	0.0	0.0	21.84495179596516	0.0
13	2.115139747283103E-4	0.0	0.0	22.74240559073738	0.0
14	2.115139747283103E-4	0.0	0.0	23.278805030648375	0.0
15	2.115139747283103E-4	0.0	0.0	24.292591511521167	0.0
16	2.115139747283103E-4	0.0	0.0	25.877677238135124	0.0
17	2.115139747283103E-4	0.0	0.0	28.3938474815031	0.0
18	2.115139747283103E-4	0.0	0.0	29.40826850430008	0.0
19	2.115139747283103E-4	0.0	0.0	30.662969402388416	0.0
20	2.115139747283103E-4	0.0	0.0	31.62853069702315	0.0
21	4.230279494566206E-4	0.0	0.0	32.73792149447314	0.0
22	4.230279494566206E-4	0.0	0.0	33.89447990828754	0.0
23	4.230279494566206E-4	0.0	0.0	34.72932556654018	0.0
24	4.230279494566206E-4	0.0	0.0	35.5034667140458	0.0
25	4.230279494566206E-4	0.0	0.0	36.20146283064922	0.0
26	4.230279494566206E-4	0.0	0.0	36.97898820175049	0.0
27	4.230279494566206E-4	0.0	0.0	37.70617324686642	0.0
28	4.230279494566206E-4	0.0	0.0	38.42637833081632	0.0
29	4.230279494566206E-4	0.0	0.0	39.143833733094745	0.0
30	4.230279494566206E-4	0.0	0.0	39.8375995702036	0.0
31	4.230279494566206E-4	0.0	0.0	40.531788435261916	0.0
32	4.230279494566206E-4	0.0	0.0	41.179021197930545	0.0
33	4.230279494566206E-4	0.0	0.0	41.82160065315515	0.0
34	4.230279494566206E-4	0.0	0.0	42.44598990655312	0.0
35	4.230279494566206E-4	0.0	0.0	43.02511516935924	0.0
36	4.230279494566206E-4	0.0	0.0	43.646331713136284	0.0
37	4.230279494566206E-4	0.0	0.0	44.26395251934295	0.0
38	4.230279494566206E-4	0.0	0.0	44.87184368271212	0.0
39	4.230279494566206E-4	0.0	0.0	45.47106277311742	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTCT	75	0.0	45.000004	1
CAACGGT	20	7.029069E-4	45.0	3
CGACCAA	20	7.029069E-4	45.0	19
TACGGTA	25	3.88731E-5	45.0	4
ATATGCG	25	3.88731E-5	45.0	1
CGCGAGG	25	3.88731E-5	45.0	2
TAACGGA	25	3.88731E-5	45.0	3
TCTAGCG	25	3.88731E-5	45.0	1
CGTAAGG	40	6.8012014E-9	45.0	2
ATGCGAG	25	3.88731E-5	45.0	1
TCGAGCG	20	7.029069E-4	45.0	1
TACCGGA	20	7.029069E-4	45.0	3
CACGGAT	30	2.1627802E-6	44.999996	4
GTTAGCG	30	2.1627802E-6	44.999996	1
CGTTTTT	5275	0.0	43.464455	1
TGACGGC	65	0.0	41.538464	3
CGGAATC	60	3.6379788E-12	41.249996	6
GCGATTA	55	6.002665E-11	40.909092	8
GCTAGCG	55	6.002665E-11	40.909092	1
CAAGCGG	55	6.002665E-11	40.909092	2
>>END_MODULE