FastQCFastQC Report
Sat 18 Jun 2016
SRR3551655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705483
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162792.3074971331697576No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTC66520.94290011240526No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCG44310.6280803364503468No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGC37200.5272983190239878No Hit
GCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGC21110.2992276213601178No Hit
GAACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCT20530.2910063034828621No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCC11390.16144967348610811No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCT9480.13437602323514528No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTT9110.12913138941689595No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC8440.11963434980006606No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGACAGAGT8270.11722465318087041No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTC8050.11410622226191135No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA7250.10276647346569655No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAC1300.045.00000418
ATTACGA207.0314325E-445.030
ATAACGC207.0314325E-445.011
CGGTCTA600.044.99999631
CGTTTTT72950.043.488691
GTCGAAT501.0804797E-940.50000443
CGATGAA5800.040.34482619
CCGATGA5650.040.2212418
CGGTTCA451.9264917E-840.041
GATGAAT6200.039.55645420
CATATTA800.039.37528
ACGGTCT750.039.030
TCACGAC700.038.5714325
CTCACGA700.038.5714324
TATTAGT700.038.5714330
TAGTACG356.244858E-638.571431
GTTTTTT84000.038.5178572
TATCACG301.1394751E-437.4999961
TACGAAT301.1394751E-437.49999612
ATAACGG301.1394751E-437.4999962