##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551655_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	705483
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01641428638252	33.0	31.0	34.0	30.0	34.0
2	32.14613676020542	33.0	31.0	34.0	30.0	34.0
3	32.072845128798285	33.0	31.0	34.0	30.0	34.0
4	35.747595618888056	37.0	35.0	37.0	35.0	37.0
5	35.769410460634774	37.0	35.0	37.0	35.0	37.0
6	35.649309763665464	37.0	35.0	37.0	33.0	37.0
7	35.919896014503536	37.0	35.0	37.0	35.0	37.0
8	35.706941201985025	37.0	35.0	37.0	35.0	37.0
9	37.547385266547884	39.0	37.0	39.0	35.0	39.0
10	37.2953706893008	39.0	37.0	39.0	34.0	39.0
11	37.32884421027863	39.0	37.0	39.0	34.0	39.0
12	37.24381452139882	39.0	37.0	39.0	34.0	39.0
13	37.29888601142763	39.0	37.0	39.0	34.0	39.0
14	38.44269387072403	40.0	38.0	41.0	34.0	41.0
15	38.46647474141829	40.0	38.0	41.0	34.0	41.0
16	38.26943810127246	40.0	38.0	41.0	34.0	41.0
17	38.23765845527107	40.0	37.0	41.0	34.0	41.0
18	37.955358243926504	39.0	37.0	41.0	33.0	41.0
19	37.66811673704398	39.0	37.0	41.0	33.0	41.0
20	37.72477153949847	39.0	35.0	41.0	34.0	41.0
21	37.62741696114577	39.0	35.0	41.0	33.0	41.0
22	37.76565842125182	39.0	35.0	41.0	33.0	41.0
23	37.80178827838516	40.0	35.0	41.0	34.0	41.0
24	37.65857717336917	40.0	35.0	41.0	33.0	41.0
25	37.31562348065084	39.0	35.0	41.0	33.0	41.0
26	37.447819437179916	39.0	35.0	41.0	33.0	41.0
27	37.52891848563325	39.0	35.0	41.0	33.0	41.0
28	37.3726879315306	39.0	35.0	41.0	33.0	41.0
29	37.35284762354302	39.0	35.0	41.0	33.0	41.0
30	37.0530204129653	39.0	35.0	41.0	33.0	41.0
31	36.86713783322915	39.0	35.0	41.0	32.0	41.0
32	36.67980093070988	39.0	35.0	41.0	31.0	41.0
33	36.40014146336623	39.0	35.0	41.0	31.0	41.0
34	36.055595953410645	39.0	35.0	41.0	29.0	41.0
35	35.89885794554936	39.0	35.0	41.0	28.0	41.0
36	35.665949427555304	39.0	35.0	41.0	26.0	41.0
37	35.59868345516476	39.0	35.0	41.0	26.0	41.0
38	35.369363967664704	38.0	35.0	41.0	25.0	41.0
39	35.271157490683684	38.0	35.0	41.0	25.0	41.0
40	35.158349669658946	38.0	35.0	40.0	24.0	41.0
41	34.972448662831	38.0	35.0	40.0	23.0	41.0
42	34.96941244509081	38.0	35.0	40.0	23.0	41.0
43	34.978893892553046	38.0	35.0	40.0	23.0	41.0
44	35.01789128866323	38.0	35.0	40.0	23.0	41.0
45	35.00602282407939	38.0	35.0	40.0	23.0	41.0
46	34.94596751445464	38.0	35.0	40.0	23.0	41.0
47	34.8804677079391	37.0	35.0	40.0	23.0	41.0
48	34.83952696237897	37.0	35.0	40.0	23.0	41.0
49	34.80121562107095	37.0	34.0	40.0	24.0	41.0
50	34.67809855092185	37.0	34.0	40.0	24.0	41.0
51	33.82576334227756	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	19.0
10	19.0
11	22.0
12	28.0
13	27.0
14	44.0
15	51.0
16	97.0
17	158.0
18	312.0
19	552.0
20	1011.0
21	1631.0
22	2163.0
23	3186.0
24	4573.0
25	7236.0
26	11253.0
27	14225.0
28	13938.0
29	12813.0
30	12592.0
31	14071.0
32	17058.0
33	23252.0
34	42192.0
35	54427.0
36	59552.0
37	84233.0
38	139968.0
39	184688.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.04448016465315	17.532102120107783	20.86740573479446	21.5560119804446
2	36.74461326495465	25.35213463683746	22.61599499917078	15.287257099037113
3	29.04166365454589	24.334675676097085	31.657176714392833	14.966483954964188
4	26.176109133742415	22.038518291723545	34.60437742652906	17.180995148004985
5	25.544768619513157	26.242588411060225	29.690155538829426	18.52248743059719
6	24.791809299444495	32.40092248856457	28.954914576254847	13.852353635736083
7	70.0575350504548	5.971795209806615	19.28409330912297	4.686576430615621
8	70.6438000632191	7.551280470259382	16.608763074375997	5.1961563921455225
9	64.31480276633171	7.141915538716029	18.930009652961164	9.61327204199109
10	39.21937169286858	20.994269174452114	24.203134590060994	15.583224542618321
11	29.8205626499859	20.90284244978263	29.93594459398738	19.340650306244093
12	24.61746066170269	18.626529625802466	34.79190852224646	21.964101190248382
13	23.470586817825517	18.80626464422247	37.65675430875017	20.066394229201837
14	20.34847048050768	22.89197613549866	34.87695663821807	21.88259674577559
15	19.7636229363429	20.654785444865436	38.023169941727865	21.558421677063798
16	21.594992366931592	21.028713661420614	34.21811723315799	23.1581767384898
17	21.340131512736665	22.70529552094097	33.86573453931562	22.088838427006745
18	21.734754770844937	21.795705920624595	33.67083260688067	22.79870670164979
19	23.158885472789564	22.975181542290883	31.06198164945151	22.803951335468042
20	24.135521337863565	23.013594941338063	32.362934330097254	20.48794939070112
21	23.47172079270514	24.92094068886139	31.395370264060222	20.211968254373243
22	22.42633770055409	21.089948304920174	32.35315379676052	24.13056019776522
23	23.47172079270514	22.564540889007954	31.796230384006417	22.167507934280486
24	23.42905498785938	22.29380438649833	31.543637479570734	22.733503146071556
25	23.02720263989352	24.649211958332092	30.134531944781095	22.189053456993292
26	21.671960911885897	22.598418388536647	32.484411389076705	23.24520931050075
27	24.782312259827663	21.813849518698536	30.683801027097747	22.720037194376054
28	22.473114164338476	23.7336689898977	31.603171160750858	22.19004568501296
29	23.844940274960557	23.3903580950923	29.814184041288026	22.950517588659118
30	24.042110157154745	24.894859266630096	29.568536732990026	21.494493843225136
31	25.173675340157025	24.255580928243486	27.413700967989307	23.15704276361018
32	25.089477705345132	25.673616515210146	27.162383785293198	22.074521994151524
33	25.09798251694229	25.24199732665422	25.98517611338615	23.674844043017337
34	24.14586885864011	26.856352314655354	26.59638857350213	22.401390253202415
35	25.667237906512273	25.613657593450164	26.03918166702812	22.679922833009442
36	25.02228969372756	29.482921629578602	24.737520252082614	20.757268424611226
37	25.745482173206156	26.724669481759307	26.077595066075297	21.452253278959237
38	23.41473855500416	28.297634386654252	25.726346347112543	22.561280711229045
39	26.04187485736722	24.776925879149463	26.64628346820547	22.534915795277847
40	23.744441751254104	25.65150400505753	28.933085559822135	21.67096868386623
41	23.89469342280395	25.339802660021576	27.39229719213645	23.373206725038024
42	22.682757770208497	25.98928677232478	28.753917528841942	22.574037928624787
43	24.387547254859438	23.56669118887344	28.583679550038767	23.462082006228357
44	24.331698992038078	23.289717824525894	28.390478579923258	23.98810460351277
45	22.837261847556924	23.12288177036158	29.01373952313521	25.026116858946285
46	24.614483977643687	24.43786739014264	28.373327209868982	22.574321422344692
47	22.58070003104256	23.92686996001321	31.07431362626739	22.418116382676835
48	22.484312166274737	23.951817407364885	29.557055237333856	24.006815189026526
49	22.678363617549962	23.347834037106495	31.23562155289355	22.738180792449995
50	22.034407632784912	22.39047574498606	31.20231104080467	24.37280558142436
51	22.08756270526717	23.006082357760572	29.645788771664233	25.260566165308024
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	250.0
1	664.0
2	1078.0
3	12915.0
4	24752.0
5	15951.0
6	7150.0
7	6947.5
8	6745.0
9	6577.5
10	6410.0
11	6068.0
12	5726.0
13	5469.5
14	5213.0
15	4831.5
16	4450.0
17	4027.5
18	3605.0
19	3339.0
20	3073.0
21	2984.0
22	2895.0
23	2943.0
24	2991.0
25	3304.0
26	4077.5
27	4538.0
28	5454.0
29	6370.0
30	7061.0
31	7752.0
32	8384.5
33	9017.0
34	9971.5
35	10926.0
36	11810.5
37	12695.0
38	13645.5
39	14596.0
40	16575.5
41	18555.0
42	21025.5
43	23496.0
44	26752.0
45	30008.0
46	46414.0
47	62820.0
48	59655.0
49	56490.0
50	55843.0
51	55196.0
52	49921.0
53	44646.0
54	41586.5
55	38527.0
56	37956.5
57	37386.0
58	36449.5
59	35513.0
60	35392.5
61	35272.0
62	33141.5
63	31011.0
64	28629.0
65	26247.0
66	22900.5
67	19554.0
68	17626.5
69	15699.0
70	13611.5
71	11524.0
72	9796.5
73	8069.0
74	6596.5
75	4188.0
76	3252.0
77	2473.0
78	1694.0
79	1286.0
80	878.0
81	624.5
82	371.0
83	283.0
84	195.0
85	123.5
86	52.0
87	47.0
88	42.0
89	26.0
90	10.0
91	6.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	705483.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.720978990787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.1979702325907	25.77738190106855
2	8.31821255473434	6.1090581692660075
3	4.264327529396031	4.697708448503589
4	3.1376210754880187	4.608660703761843
5	2.4599238644327293	4.516540627238492
6	2.0148902886677895	4.439324637534639
7	1.702418141359413	4.376012256167558
8	1.4398246672172366	4.229741708424084
9	1.1669081267188213	3.8565007924878416
>10	5.2067214931170245	27.176349023973295
>50	0.052381164896800995	1.3645363781378932
>100	0.02987666326344668	2.0739470540670553
>500	0.006208137820975874	1.590083067009358
>1k	0.0019400430690549603	1.916933475227948
>5k	3.880086138109921E-4	0.9477806954969757
>10k+	3.880086138109921E-4	2.319441061634887
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16279	2.3074971331697576	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTC	6652	0.94290011240526	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCG	4431	0.6280803364503468	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGC	3720	0.5272983190239878	No Hit
GCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGC	2111	0.2992276213601178	No Hit
GAACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCT	2053	0.2910063034828621	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCC	1139	0.16144967348610811	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCT	948	0.13437602323514528	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTT	911	0.12913138941689595	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	844	0.11963434980006606	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGACAGAGT	827	0.11722465318087041	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTC	805	0.11410622226191135	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	725	0.10276647346569655	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09879756138702138	0.0
2	0.0	0.0	0.0	0.5373623460806285	0.0
3	0.0	0.0	0.0	0.7966173529340891	0.0
4	0.0	0.0	0.0	1.3769289975803811	0.0
5	0.0	0.0	0.0	3.0326740686876934	0.0
6	0.0	0.0	0.0	4.232135997607313	0.0
7	0.0	0.0	0.0	5.20026705108415	0.0
8	0.0	0.0	0.0	6.5936386844190435	0.0
9	0.0	0.0	0.0	7.429803411279932	0.0
10	0.0	0.0	0.0	9.142247226368317	0.0
11	0.0	0.0	0.0	10.139011145555598	0.0
12	0.0	0.0	0.0	11.427490102525503	0.0
13	0.0	0.0	0.0	11.83217738769042	0.0
14	0.0	0.0	0.0	12.068469403231544	0.0
15	0.0	0.0	0.0	12.376343583048776	0.0
16	0.0	0.0	0.0	12.715827312635456	0.0
17	0.0	0.0	0.0	13.07657307121504	0.0
18	0.0	0.0	0.0	13.499262207593945	0.0
19	0.0	0.0	0.0	13.87007199323017	0.0
20	0.0	0.0	0.0	14.142084217479372	0.0
21	0.0	0.0	0.0	14.416364391487818	0.0
22	0.0	0.0	0.0	14.725372546184671	0.0
23	0.0	0.0	0.0	15.032679738562091	0.0
24	0.0	0.0	0.0	15.293352214015078	0.0
25	0.0	0.0	0.0	15.532195673035353	0.0
26	0.0	0.0	0.0	15.749210115622914	0.0
27	0.0	0.0	0.0	15.990038030682525	0.0
28	1.417468599526849E-4	0.0	0.0	16.216974753466772	0.0
29	1.417468599526849E-4	0.0	0.0	16.446746413450075	0.0
30	1.417468599526849E-4	0.0	0.0	16.734067298574168	0.0
31	1.417468599526849E-4	0.0	0.0	16.96483118657714	0.0
32	1.417468599526849E-4	0.0	0.0	17.2213930030915	0.0
33	1.417468599526849E-4	0.0	0.0	17.456551043753002	0.0
34	1.417468599526849E-4	0.0	0.0	17.69185083127446	0.0
35	1.417468599526849E-4	0.0	0.0	17.920488516378143	0.0
36	1.417468599526849E-4	0.0	0.0	18.165852330956238	0.0
37	1.417468599526849E-4	0.0	0.0	18.41943746341159	0.0
38	1.417468599526849E-4	0.0	0.0	18.696836068338996	0.0
39	2.834937199053698E-4	0.0	0.0	18.983448219163325	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGTAC	130	0.0	45.000004	18
ATTACGA	20	7.0314325E-4	45.0	30
ATAACGC	20	7.0314325E-4	45.0	11
CGGTCTA	60	0.0	44.999996	31
CGTTTTT	7295	0.0	43.48869	1
GTCGAAT	50	1.0804797E-9	40.500004	43
CGATGAA	580	0.0	40.344826	19
CCGATGA	565	0.0	40.22124	18
CGGTTCA	45	1.9264917E-8	40.0	41
GATGAAT	620	0.0	39.556454	20
CATATTA	80	0.0	39.375	28
ACGGTCT	75	0.0	39.0	30
TCACGAC	70	0.0	38.57143	25
CTCACGA	70	0.0	38.57143	24
TATTAGT	70	0.0	38.57143	30
TAGTACG	35	6.244858E-6	38.57143	1
GTTTTTT	8400	0.0	38.517857	2
TATCACG	30	1.1394751E-4	37.499996	1
TACGAAT	30	1.1394751E-4	37.499996	12
ATAACGG	30	1.1394751E-4	37.499996	2
>>END_MODULE