FastQCFastQC Report
Sat 18 Jun 2016
SRR3551654_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551654_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553438
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG42470.7673849645308056No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC41160.7437147431148565No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC37310.6741495885718003No Hit
GCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC26390.47683751386785866No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24440.44160321481358344No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT8890.16063226594487548No Hit
CTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGCT8800.15900606752698587No Hit
CCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC7900.14274408334808958No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCC7540.13623928967653107No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA7520.13587791225033338No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT7320.1322641379883564No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTA6740.12178419262862325No Hit
TCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC6640.11997730549763479No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCCTAAT6540.11817041836664631No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTC6390.1154600876701636No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5620.10154705676155232No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATACG253.888174E-545.0000041
GTCGTAA302.1634532E-645.0000049
CGGTCTA253.888174E-545.00000431
CTTAACG207.0301135E-445.01
CGTATGT207.0301135E-445.024
TTCGTAA207.0301135E-445.026
TCTAGCG207.0301135E-445.01
TACGAAT800.045.012
ACGTAGG351.2104101E-745.02
ATGTTCG207.0301135E-445.027
GCTACGA900.042.510
CGAATAT800.042.187514
CCATTAC451.9252184E-840.06
CGTTTTT11850.039.6835441
CGTATGG403.453897E-739.3752
CCAATCG403.453897E-739.37540
ATGCGAG403.453897E-739.3751
GCGTAAG403.453897E-739.3751
GTGTAAG750.039.0000041
AGCTACG1050.038.571439