##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551654_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	553438
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.994546814638678	33.0	31.0	34.0	30.0	34.0
2	32.078702582764464	33.0	31.0	34.0	30.0	34.0
3	32.06653500482439	33.0	31.0	34.0	30.0	34.0
4	35.69613579118167	37.0	35.0	37.0	33.0	37.0
5	35.74378521171297	37.0	35.0	37.0	35.0	37.0
6	35.63208886993665	37.0	35.0	37.0	33.0	37.0
7	35.933072900668186	37.0	35.0	37.0	35.0	37.0
8	35.69283641528048	37.0	35.0	37.0	35.0	37.0
9	37.51166887709192	39.0	37.0	39.0	35.0	39.0
10	37.18590881002027	39.0	37.0	39.0	34.0	39.0
11	37.26522031374788	39.0	37.0	39.0	34.0	39.0
12	37.1789830116472	39.0	37.0	39.0	34.0	39.0
13	37.20477271166779	39.0	37.0	39.0	34.0	39.0
14	38.261357189061826	40.0	38.0	41.0	33.0	41.0
15	38.33849681445799	40.0	38.0	41.0	34.0	41.0
16	38.15036372637947	40.0	37.0	41.0	34.0	41.0
17	38.13620315193391	40.0	37.0	41.0	34.0	41.0
18	37.980321192256405	40.0	37.0	41.0	33.0	41.0
19	37.82402364853877	39.0	37.0	41.0	33.0	41.0
20	38.0394804838121	40.0	37.0	41.0	34.0	41.0
21	37.943045110744116	40.0	36.0	41.0	34.0	41.0
22	38.11051825136691	40.0	37.0	41.0	34.0	41.0
23	38.118437837662036	40.0	37.0	41.0	34.0	41.0
24	38.00498700848153	40.0	36.0	41.0	34.0	41.0
25	37.64416610352018	39.0	36.0	41.0	33.0	41.0
26	37.83152765079377	40.0	36.0	41.0	33.0	41.0
27	37.894495860421586	40.0	36.0	41.0	34.0	41.0
28	37.79344569762105	40.0	36.0	41.0	33.0	41.0
29	37.845789410918655	40.0	36.0	41.0	33.0	41.0
30	37.59208980951796	40.0	36.0	41.0	33.0	41.0
31	37.535561345624984	40.0	36.0	41.0	33.0	41.0
32	37.53201984684825	40.0	36.0	41.0	33.0	41.0
33	37.51262472038422	40.0	36.0	41.0	33.0	41.0
34	37.415222662701154	40.0	36.0	41.0	33.0	41.0
35	37.36077392589595	40.0	35.0	41.0	33.0	41.0
36	37.215742323440026	40.0	35.0	41.0	33.0	41.0
37	37.20313747881425	40.0	35.0	41.0	33.0	41.0
38	37.031143506589714	39.0	35.0	41.0	32.0	41.0
39	36.94618186680351	39.0	35.0	41.0	32.0	41.0
40	36.878152566321795	39.0	35.0	41.0	32.0	41.0
41	36.673481040333336	39.0	35.0	41.0	31.0	41.0
42	36.68255703439229	39.0	35.0	41.0	32.0	41.0
43	36.69657667164163	39.0	35.0	41.0	32.0	41.0
44	36.77015672938974	39.0	35.0	41.0	32.0	41.0
45	36.73947217213129	39.0	35.0	41.0	32.0	41.0
46	36.64334035610131	39.0	35.0	41.0	32.0	41.0
47	36.56868881428453	39.0	35.0	41.0	32.0	41.0
48	36.529423350041014	39.0	35.0	41.0	32.0	41.0
49	36.50303376349293	39.0	35.0	41.0	32.0	41.0
50	36.3936574647928	38.0	35.0	41.0	31.0	41.0
51	35.53086524597155	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	24.0
10	17.0
11	14.0
12	14.0
13	20.0
14	15.0
15	32.0
16	51.0
17	86.0
18	128.0
19	237.0
20	453.0
21	815.0
22	1267.0
23	1765.0
24	2327.0
25	3046.0
26	3919.0
27	4605.0
28	5071.0
29	5805.0
30	7213.0
31	9217.0
32	12281.0
33	17592.0
34	33479.0
35	43533.0
36	44405.0
37	68127.0
38	117840.0
39	169947.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.847007252844946	19.71115102324018	22.993722874106943	13.448118849807928
2	29.881576617435012	27.775830354981046	25.17066048952186	17.171932538062077
3	30.70208406361688	25.99080655827753	26.683205706872315	16.623903671233272
4	27.740776744639867	25.145364069688025	28.586399921942473	18.527459263729632
5	27.94712325499875	28.470036390706817	24.19042422096061	19.392416133333814
6	26.38163624471034	35.16563734329771	23.472909341245092	14.97981707074686
7	75.66050759073283	6.728666987087985	12.571417213852321	5.039408208326859
8	75.83216186817674	8.396604497703446	9.74454229742085	6.026691336698962
9	69.43487798091205	8.136051373414908	12.156555928577365	10.272514717095682
10	41.27454204445665	23.073767974009733	18.735974038645704	16.915715942887914
11	31.094901325893776	22.978725710919743	24.796815542120346	21.129557421066135
12	27.04783552990579	20.488835244417622	29.78075231552586	22.682576910150733
13	24.52831211445546	22.473700757808462	32.4244088768751	20.573578250860983
14	20.872256693613377	26.963815278314822	28.7548018025506	23.409126225521195
15	18.948102587823747	23.207116244276683	35.35174671778953	22.493034450110038
16	21.672888381354372	22.743107629038846	30.40557388542167	25.17843010418511
17	21.23544100694206	24.568244320050304	29.064140879375827	25.132173793631807
18	22.366913728367045	23.623965105395726	29.98565331617995	24.02346785005728
19	24.18518424828075	24.6004069109819	26.69621529421543	24.518193546521925
20	24.850118712484505	24.90559014740585	28.599590197998694	21.64470094211095
21	24.446098749995482	27.036090763554366	27.172149364517793	21.34566112193236
22	22.672458342217194	22.83074165489179	27.356813229304816	27.1399867735862
23	23.031306126431506	25.534025491563643	27.2818274133688	24.152840968636053
24	23.390334599358916	23.894094731478503	27.59134717890712	25.12422349025546
25	22.29662581897159	27.286886697335568	26.236723896805064	24.17976358688778
26	20.98482576187396	25.27943509480737	27.836180385156062	25.899558758162613
27	24.303354666647394	24.741344107199	27.33476920630676	23.62053201984685
28	22.061369114516893	24.454229742084934	28.909652029676312	24.57474911372186
29	22.65926806616098	22.890730307640602	27.121195147423922	27.3288064787745
30	22.884044825255945	24.49651090095006	27.821363910681956	24.79808036311204
31	25.179514236463707	24.301909156942603	24.129351435933202	26.38922517066049
32	24.49687227837626	25.595098276591056	23.662271112572682	26.245758332460007
33	23.596681109717803	23.678713785464677	24.511869441563462	28.21273566325406
34	22.451476046097305	23.44652878913266	26.649416917522828	27.452578247247207
35	21.956208283493364	24.372016377624956	26.384888641546116	27.286886697335568
36	23.473632096097486	26.53196925400858	24.92456246228123	25.069836187612704
37	22.63758542058912	25.377729754733142	28.498765896089534	23.485918928588205
38	22.463762878588025	26.805712654353332	24.545477542199848	26.18504692485879
39	22.26103014249112	24.747487523444363	27.043137623365222	25.9483447106993
40	21.422634513712467	24.628232972799122	28.776303759409366	25.172828754079045
41	20.394696424893123	26.173121469794268	27.247135180453817	26.18504692485879
42	21.549658679020954	26.101026673267828	27.937727441917616	24.4115872057936
43	22.92921700353066	24.396047976467102	27.20882917327686	25.465905846725377
44	23.119843595849943	22.876275210592695	27.67898120476006	26.324899988797302
45	22.556456188407733	22.26988388943296	27.44282105673987	27.730838865419432
46	24.102428817681478	25.027012962608282	26.927496846981956	23.94306137272829
47	20.958987275900824	23.98570390901962	30.53747664598383	24.517832169095723
48	21.521109862351338	24.32088147181798	27.718371344215615	26.439637321615066
49	22.149183829082933	22.68420310856862	30.201395639619978	24.96521742272847
50	21.62283760782599	22.328788409903186	29.488036600305723	26.5603373819651
51	21.475756995363525	22.72774908842544	26.987485499730774	28.809008416480257
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	126.0
1	531.0
2	936.0
3	2468.0
4	4000.0
5	2712.5
6	1425.0
7	1364.0
8	1303.0
9	1312.0
10	1321.0
11	1338.0
12	1355.0
13	1345.5
14	1336.0
15	1258.0
16	1180.0
17	1171.5
18	1163.0
19	1191.5
20	1220.0
21	1384.5
22	1549.0
23	1621.5
24	1694.0
25	1929.5
26	2891.5
27	3618.0
28	4715.0
29	5812.0
30	6543.0
31	7274.0
32	8073.5
33	8873.0
34	9746.5
35	10620.0
36	11827.5
37	13035.0
38	13863.5
39	14692.0
40	16681.5
41	18671.0
42	21337.0
43	24003.0
44	27545.0
45	31087.0
46	44210.0
47	57333.0
48	55354.5
49	53376.0
50	52442.0
51	51508.0
52	44751.0
53	37994.0
54	34694.0
55	31394.0
56	29968.5
57	28543.0
58	28206.5
59	27870.0
60	26262.5
61	24655.0
62	22699.0
63	20743.0
64	19128.0
65	17513.0
66	15393.5
67	13274.0
68	11352.5
69	9431.0
70	8627.5
71	7824.0
72	6963.5
73	6103.0
74	4689.0
75	2525.5
76	1776.0
77	1510.5
78	1245.0
79	930.0
80	615.0
81	462.5
82	310.0
83	223.0
84	136.0
85	91.5
86	47.0
87	29.0
88	11.0
89	6.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	553438.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.7621986474165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.13125389786303	22.49678159740715
2	9.256436489764573	5.69496676130665
3	3.2624546099115226	3.0108083036483393
4	1.7462533044461808	2.1487436416032355
5	1.1937046629440184	1.8360489983915627
6	0.9414070679276878	1.7375850739004068
7	0.7674373301543862	1.652564171975653
8	0.7073704017052048	1.7408215051646654
9	0.6215567598699275	1.720840726598694
>10	8.232793749358414	48.92723275057373
>50	0.08442557977062456	1.7903758057024568
>100	0.044279150229348536	2.4805186401180923
>500	0.007675052706420414	1.6430847955153303
>1k	0.002951943348623236	3.1196272280940454
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	4247	0.7673849645308056	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	4116	0.7437147431148565	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	3731	0.6741495885718003	No Hit
GCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	2639	0.47683751386785866	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2444	0.44160321481358344	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT	889	0.16063226594487548	No Hit
CTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGCT	880	0.15900606752698587	No Hit
CCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	790	0.14274408334808958	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCC	754	0.13623928967653107	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	752	0.13587791225033338	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT	732	0.1322641379883564	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTA	674	0.12178419262862325	No Hit
TCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	664	0.11997730549763479	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCCTAAT	654	0.11817041836664631	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTC	639	0.1154600876701636	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	562	0.10154705676155232	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16587223862474207	0.0
2	0.0	0.0	0.0	0.9963175640270455	0.0
3	0.0	0.0	0.0	1.3282427299896284	0.0
4	0.0	0.0	0.0	1.9228892847979357	0.0
5	0.0	0.0	0.0	3.820482149762033	0.0
6	0.0	0.0	0.0	4.790057784250449	0.0
7	0.0	0.0	0.0	5.7800512433190345	0.0
8	0.0	0.0	0.0	7.365775389474521	0.0
9	0.0	0.0	0.0	8.046610460430978	0.0
10	0.0	0.0	0.0	10.041955919181552	0.0
11	0.0	0.0	0.0	11.397121267422909	0.0
12	0.0	0.0	0.0	13.205815285542373	0.0
13	0.0	0.0	0.0	13.664583928100347	0.0
14	0.0	0.0	0.0	13.87888074183558	0.0
15	0.0	0.0	0.0	14.331867345574391	0.0
16	0.0	0.0	0.0	14.994994922647162	0.0
17	0.0	0.0	0.0	15.729313852680878	0.0
18	0.0	0.0	0.0	16.5727687654263	0.0
19	0.0	0.0	0.0	17.18277386084801	0.0
20	0.0	0.0	0.0	17.674247160476874	0.0
21	0.0	0.0	0.0	18.23908007762387	0.0
22	0.0	0.0	0.0	18.826499083908224	0.0
23	0.0	0.0	0.0	19.412833957913985	0.0
24	0.0	0.0	0.0	19.89220111376523	0.0
25	0.0	0.0	0.0	20.32603471391556	0.0
26	1.8068871309884758E-4	0.0	0.0	20.68976109338354	0.0
27	1.8068871309884758E-4	0.0	0.0	21.095045876864255	0.0
28	1.8068871309884758E-4	0.0	0.0	21.493645177960314	0.0
29	1.8068871309884758E-4	0.0	0.0	21.87381423032029	0.0
30	3.6137742619769516E-4	0.0	0.0	22.31541744513387	0.0
31	3.6137742619769516E-4	0.0	0.0	22.714920189795425	0.0
32	3.6137742619769516E-4	0.0	0.0	23.102316790679353	0.0
33	5.420661392965427E-4	0.0	0.0	23.49296578839906	0.0
34	7.227548523953903E-4	0.0	0.0	23.884518229684264	0.0
35	9.034435654942378E-4	0.0	0.0	24.311485658736842	0.0
36	9.034435654942378E-4	0.0	0.0	24.679187189892996	0.0
37	9.034435654942378E-4	0.0	0.0	25.05827210997438	0.0
38	9.034435654942378E-4	0.0	0.0	25.436995652629562	0.0
39	9.034435654942378E-4	0.0	0.0	25.805058561211915	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATACG	25	3.888174E-5	45.000004	1
GTCGTAA	30	2.1634532E-6	45.000004	9
CGGTCTA	25	3.888174E-5	45.000004	31
CTTAACG	20	7.0301135E-4	45.0	1
CGTATGT	20	7.0301135E-4	45.0	24
TTCGTAA	20	7.0301135E-4	45.0	26
TCTAGCG	20	7.0301135E-4	45.0	1
TACGAAT	80	0.0	45.0	12
ACGTAGG	35	1.2104101E-7	45.0	2
ATGTTCG	20	7.0301135E-4	45.0	27
GCTACGA	90	0.0	42.5	10
CGAATAT	80	0.0	42.1875	14
CCATTAC	45	1.9252184E-8	40.0	6
CGTTTTT	1185	0.0	39.683544	1
CGTATGG	40	3.453897E-7	39.375	2
CCAATCG	40	3.453897E-7	39.375	40
ATGCGAG	40	3.453897E-7	39.375	1
GCGTAAG	40	3.453897E-7	39.375	1
GTGTAAG	75	0.0	39.000004	1
AGCTACG	105	0.0	38.57143	9
>>END_MODULE